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南极土壤嗜冷真菌Chrysosporium sp.C_(3438)活性代谢产物C_(3438)A的分离及结构鉴别 被引量:12
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作者 鲁敏 王文翔 +1 位作者 王丽萍 胡继兰 《中国抗生素杂志》 CAS CSCD 北大核心 2002年第1期11-12,38,共3页
以对精原细胞法是否有活性作为筛选模型 ,对南极土壤嗜冷真菌 Chrysosporium sp. C3 4 3 8经低温发酵的活性产物进行了深入的化学研究 ,首次从南极土壤微生物代谢产物中分离得到
关键词 南极土壤嗜冷真菌Chrysosporiumsp C3438 FERRICHROME 结构鉴别 精原细胞法 活性代谢产物
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南极土壤微生物宏基因组文库构建及其抗肿瘤活性初探 被引量:14
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作者 赵晶 杨祥胜 曾润颖 《自然科学进展》 北大核心 2007年第2期267-271,共5页
微生物代谢产物是药物的重要来源之一,然而采用传统的分离培养筛选的手段仅能对不到1%的微生物资源进行研究开发.通过构建环境样品微生物宏基因组文库能突破人工培养的瓶颈,直接克隆到完整的次级代谢产物合成基因簇,并从中寻找新的药... 微生物代谢产物是药物的重要来源之一,然而采用传统的分离培养筛选的手段仅能对不到1%的微生物资源进行研究开发.通过构建环境样品微生物宏基因组文库能突破人工培养的瓶颈,直接克隆到完整的次级代谢产物合成基因簇,并从中寻找新的药物和活性次生代谢产物.实验直接提取南极中山站排污口土壤样品中微生物总基因组DNA,以黏粒(SuperCos 1)为载体,构建了宏基因组文库,获得了约27 000个克隆子,平均插入片段在35 kb以上,覆盖了至少946 Mb的微生物基因组信息.通过差异性DNA修复试验(DDRT)法对该文库进行筛选,获得13个具有抗肿瘤活性的克隆子,并采用MTT法对其中活性较高的克隆子进行细胞毒活性测定,表明克隆子AE-3对卵巢癌细胞具有明显的生长抑制作用. 展开更多
关键词 南极土壤微生物 宏基因组文库 抗肿瘤活性 次生代谢产物
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人类活动对南极土壤生态系统的影响研究 被引量:2
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作者 郑先昌 《广州大学学报(自然科学版)》 CAS 2007年第2期37-40,共4页
南极土壤生态系统具有其独有的特性,蕴藏着许多具有全球性意义的重要信息,是一种宝贵的科研资源,但由于其结构简单、极端脆弱,易遭受永久性破坏.文章研究了南极土壤生态系统的特点,从人类输入物、人类工程活动和科考队员的野外考察等方... 南极土壤生态系统具有其独有的特性,蕴藏着许多具有全球性意义的重要信息,是一种宝贵的科研资源,但由于其结构简单、极端脆弱,易遭受永久性破坏.文章研究了南极土壤生态系统的特点,从人类输入物、人类工程活动和科考队员的野外考察等方面分析了人类在南极地区的活动对当地土壤生态系统的影响. 展开更多
关键词 南极土壤生态系统 人类活动 影响
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南极土壤来源的恶臭假单胞菌1A00316抗南方根结线虫的机制 被引量:14
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作者 唐佳频 邵宗泽 +4 位作者 张智涛 李光玉 景雪萍 喻子牛 张吉斌 《应用与环境生物学报》 CAS CSCD 北大核心 2014年第6期1046-1051,共6页
对1 432株海洋和极地来源的细菌进行抗南方根结线虫的离体筛选,并对其中活性较好的一株菌进行菌种鉴定并通过盆栽实验验证其在实际生防中的效果.设计诱导系统抗性实验,测定植物防御酶活的变化,初步研究细菌的抗虫机制.结果显示,从1 432... 对1 432株海洋和极地来源的细菌进行抗南方根结线虫的离体筛选,并对其中活性较好的一株菌进行菌种鉴定并通过盆栽实验验证其在实际生防中的效果.设计诱导系统抗性实验,测定植物防御酶活的变化,初步研究细菌的抗虫机制.结果显示,从1 432株细菌离体筛选得到了9株具有抗南方根结线虫的细菌,其中南极土壤来源的菌株1A00316在离体和活体中均表现出较好的抗性,其生防效果达到71.67%,经鉴定该菌株为恶臭假单胞菌(Pseudomonas putida).诱导番茄系统抗性实验结果显示,经菌株1A00316发酵液处理后的番茄3种防御酶苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)以及过氧化物酶(POD)的酶活均显著提高,分析数据认为该菌株的菌体可诱导番茄系统抗性.本研究表明恶臭假单胞菌1A00316抗南方根结线虫效果较好,可作为微生物制剂应用于实际生防中;该菌不仅有直接抗虫作用,还存在间接抗虫机制,其多重抗虫机制也具有进一步的研究价值. 展开更多
关键词 南方根结线虫 恶臭假单胞菌 南极土壤 生物防治 诱导系统抗性
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南极海洋动物粪土源N_2O稳定同位素实验研究 被引量:1
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作者 白波 朱仁斌 +1 位作者 徐华 刘雅淑 《极地研究》 CAS CSCD 北大核心 2012年第3期215-225,共11页
N2O是一种重要的温室气体,土壤是全球N2O的重要排放源。通过测定土壤源N2O中N、O同位素值,可以有效识别N2O的来源途径。本文采集了南极法尔兹半岛两个地点的海豹粪土(HS和GS)、阿德雷岛两个地点的企鹅粪土(AB和AF)以及东南极的帝企鹅粪... N2O是一种重要的温室气体,土壤是全球N2O的重要排放源。通过测定土壤源N2O中N、O同位素值,可以有效识别N2O的来源途径。本文采集了南极法尔兹半岛两个地点的海豹粪土(HS和GS)、阿德雷岛两个地点的企鹅粪土(AB和AF)以及东南极的帝企鹅粪土(DQ和DQT),在室内对所采集的样品分别在有氧和厌氧条件下进行冻融培养实验。结果表明:土壤在厌氧条件下比有氧条件下排放了更多的N2O。土壤排放的N2O与当地大气N2O相比普遍贫15N和18O。除DQT和HS外,δ15N和δ18O在有氧和厌氧培养下均呈现很好的正相关性。N2O排放量下降的同时伴随着培养瓶内剩余N2O中δ15N和δ18O值的增加,证实N2O还原为N2的过程会引起重同位素富集。高的水分含量有利于土壤反硝化作用的进行,使释放的N2O气体富集重同位素;pH值也会影响N2O的同位素组成,低pH会引起δ15N值增加。 展开更多
关键词 N2O 南极土壤 硝化作用 反硝化作用 δ15N和δ18O
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基于宏基因组技术解析南极不同纬度地区土壤抗生素抗性基因的分布与迁移 被引量:4
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作者 李毅 张利环 +8 位作者 冯焱 李宏 张锋 白芸 丁云云 康美琳 王雨竹 雷嘉轩 申少斐 《微生物学报》 CAS CSCD 北大核心 2021年第11期3744-3756,共13页
【目的】南极洲不同地区环境极端多样,且受人类活动影响不一。本研究旨在探究南极不同纬度地区土壤抗生素抗性基因(ARGs)的分布特征与迁移机制。【方法】下载南极不同纬度地区及加拿大阿尔伯特地区养殖场附近土壤宏基因组数据集,利用Met... 【目的】南极洲不同地区环境极端多样,且受人类活动影响不一。本研究旨在探究南极不同纬度地区土壤抗生素抗性基因(ARGs)的分布特征与迁移机制。【方法】下载南极不同纬度地区及加拿大阿尔伯特地区养殖场附近土壤宏基因组数据集,利用MetaWRAP进行组装,使用CARD、PlasFlow和ICEberg数据库对ARGs与可移动遗传元件(MGEs)进行注释。【结果】在南极不同纬度地区土壤中,优势菌门为变形菌门、放线菌门、拟杆菌门和厚壁菌门。共注释出25类406种ARGs,以多重耐药类、四环素类及氨基糖苷类抗生素抗性基因为主。NMDS分析结果表明,南极不同纬度地区与养殖场附近土壤中ARGs的分布特征显著不同(ANISOM,P=0.001)。南极高纬度地区ARGs占总基因数的比例为0.28%,显著低于低纬度地区(1.93%,P<0.01)。不同抗生素类型的ARGs呈现不同的区域分布模式,其中硝基咪唑类、氨基糖苷类、糖肽类与大环内酯类ARGs主要分布在南极高纬度地区,四环素类与磺胺类ARGs主要分布在南极低纬度地区(P<0.05)。南极土壤中ARGs的迁移研究表明,质粒携带的ARGs占检测到的ARGs的17%。同时,共发现163个整合与接合元件(ICEs)可携带多抗耐药类、肽类和四环素类等14类ARGs。这些携带ARGs的ICEs主要分布于α-、β-与γ-变形菌纲中。【结论】南极高纬度与南极低纬度地区土壤中ARGs的分布存在差异性,质粒与ICEs共同介导ARGs的迁移。本研究为进一步了解抗生素时代之前的原始抗性组提供数据基础。 展开更多
关键词 抗生素抗性基因 宏基因组学 微生物多样性 可移动遗传元件 南极土壤
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东南极格罗夫山地区的土壤特征 被引量:3
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作者 李潇丽 刘小汉 +1 位作者 琚宜太 黄费新 《中国科学(D辑)》 CSCD 北大核心 2002年第9期767-776,共10页
在东南极格罗夫山地区哈丁山南岭发现的三个土壤地点,是人类首次在东南极内陆地区发现的土壤,属南极寒漠土壤(cold desert soil),其主要特征是地表发育漠境砾幂(desert pavement),土壤中含有丰富的盐分,具有较强的染色作用,土壤呈... 在东南极格罗夫山地区哈丁山南岭发现的三个土壤地点,是人类首次在东南极内陆地区发现的土壤,属南极寒漠土壤(cold desert soil),其主要特征是地表发育漠境砾幂(desert pavement),土壤中含有丰富的盐分,具有较强的染色作用,土壤呈弱酸性,不含有机质.据 1:5的土水提取液分析,主要阳离子为 Mg2+和 Na+,其次是 K+和 Ca2+,主要阴离子为 SO42-,其次为Cl-和 NO3-.本区土壤中盐分层的分布与土壤中最大水分层和土壤中粘粒级含量最大层相一致.土壤中有粘粒迁移,这是本地区土壤与其他寒漠土壤的不同.部分土壤剖面上部具有较强的染色作用,由土壤中暗色矿物的风化所致,染色程度与土壤中游离铁含量有很大关系.土壤特征指示其年龄在0.5~3.5 Ma之间,且在哈丁山上也没有发现冰川运动的遗迹,说明至少在0.5 Ma以来,即使在末次冰期极盛期,该区的冰盖表面也没有到达过哈丁山土壤采样点的高度. 展开更多
关键词 南极 格罗夫山地区 土壤特征 南极寒漠土壤 漠境砾幂 可溶盐 土壤类型
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Evaporation from Bare Soil in Extremely Arid Environment in Southern Israel 被引量:6
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作者 WANGXUEFENG XUFUAN 《Pedosphere》 SCIE CAS CSCD 1996年第2期139-146,共8页
Microlysimeters of different sizes(5 cm 10 cm and 15 cm in length)were used extensively in the present study for the measurements of soil evapondion in site in an extremely arid area in southern Israel.All of the data... Microlysimeters of different sizes(5 cm 10 cm and 15 cm in length)were used extensively in the present study for the measurements of soil evapondion in site in an extremely arid area in southern Israel.All of the data obtained from the microlysimeters were used to evaluate two conventional eVaporation models developed by Black et al.and Ritchie,respectively.Our results indicated that the models could overestimate total cumulative evaporation by about 30% in the extremely arid environment.Reducing the power factor of the conventional model by a faCtor of 0.1 produced good agreement between the measured and simulated cumulative evaporation.Microlysimeter method proved to be a simple and accurate approach for the evaluation of soil evaporstion. 展开更多
关键词 bare soil EVAPORATION microlysimeter soil water
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Role of Lichens in Weathering and Soil-Forming Pro-cesses in Fildes Peninsula,Antarctic 被引量:7
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作者 CHENJIE GONGZi-TONG 《Pedosphere》 SCIE CAS CSCD 1995年第4期305-314,共10页
Lichens play an unparalleledly vital role in weathering and soil-forming processes in Antarctic region. Inthis study some related chemical components and micromorphological analyses have been carried out on thesamples... Lichens play an unparalleledly vital role in weathering and soil-forming processes in Antarctic region. Inthis study some related chemical components and micromorphological analyses have been carried out on thesamples of the weathered rocks and the lichens grown on them from Fildes Peninsula, Antarctic. The resultsindicated that the major chemical components in the bioweathering surface layer of the sampled rocks havebeen obviously altered and the weathering potential in this layer has greatly decreased by an average rangearound 4.66 percent in 4 samples. In the weathering surface layer ferruginmation of some minerals in varyingdegrees was seen by means of microscopic examination through the thin section of the weathered rocks, andits products proved to be dominated by hematite, limonite, goethite and free iron oxides Meanwhile, thestudy suggested that the dissolntion and absorption of lichens by their secretion accelerated the process ofcalcitization of minerals in the bicaweathering surface layer. Eventually, the results aIso show that differentspeciesof lichens play different roles in weathering and soilforming processes. 展开更多
关键词 ANTARCTIC LICHENS weathering and soil-forming processesf weathering potential
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Mrakia psychrophila sp.nov.,a new species isolated from Antarctic soil
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作者 XIN Ming-xiu ZHOU Pei-jin 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2007年第4期260-265,共6页
The yeast strain (Y18) was isolated from a soil sample collected from Fildes Peninsula, Antarctica. The strain is a psychrophilic yeast with optimum and maximum growth temperatures of 10 ℃ and 18 ℃, respectively. ... The yeast strain (Y18) was isolated from a soil sample collected from Fildes Peninsula, Antarctica. The strain is a psychrophilic yeast with optimum and maximum growth temperatures of 10 ℃ and 18 ℃, respectively. Teliospores were formed after 7 d on malt agar, when the germination of teliospores was observed. Both inositol and D-glucuronate were assimilated. Positive results of the DBB (diazonium blue B) color reaction, urease test, and starch formation were observed. The major CoQ is Qs. All results indicated that Y18 belongs to the genes of Mrakia. The 18S rDNA sequence analyses showed that Y18 is closely related to Mrakiafrigida. DNA-DNA relatedness study, and some biochemistry characteristics indicated that Y18 represents a new species for which Mrakia psychrophila sp. nov. is proposed. 展开更多
关键词 Mrakiapsychrophila Psychrophilic yeast 18S rDNA sequence Teliospores
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Behaviours of Main Elements in Soil-Forming Processesof Fildes Peninsula, the Maritime Antarctic 被引量:1
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作者 CHENJIE H.P.BLUME 《Pedosphere》 SCIE CAS CSCD 1999年第2期113-122,共10页
Nine horizon samples of three soil profiles representative of Dystri-gelic Cambisol, Fibri-gelic Histosoland Relic Ornithosol were taken from Fildes Peninsula of King George Island, Antarctica, to quantitativelydemons... Nine horizon samples of three soil profiles representative of Dystri-gelic Cambisol, Fibri-gelic Histosoland Relic Ornithosol were taken from Fildes Peninsula of King George Island, Antarctica, to quantitativelydemonstrate the enrichment, migration and loss of the elements (Si, Al, Fe, Ca, Mg, Mn, K, Na and P) insoil horizons and their behaviours within profiles by application of the concept of the enrichment coefficientcalculated in terms of the abundance of Ti. Besides, six horizon samples of 4 other profiles randomly takenfrom the studied area were examined for calculation of the redistributing enrichment coefficient, which wasapplied for a better understanding of the element redistribution between soil and clay fraction along with soildevelopment. The results showed that the enrichment, migration and redistribution of the elements variedstrongly among the investigated soils and horizons, due to the differences of weathering degrees and moisturestatus, influences of sea bird activities, etc. 展开更多
关键词 ELEMENTS ENRICHMENT migration soil-forming process the maritime Antarctic
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Rock Weathering Tendency at Different Stages of Soil-Forming Processes in Fildes Peninsula, Antarctic 被引量:1
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作者 CHENJIE GONGZITONG 《Pedosphere》 SCIE CAS CSCD 1996年第1期29-34,共6页
From the view of energy state of material, this paper introduces a concept of weathering potential in carrying out quantitative calculation of the relevant products at different stages of rock-weathering and primary s... From the view of energy state of material, this paper introduces a concept of weathering potential in carrying out quantitative calculation of the relevant products at different stages of rock-weathering and primary soil-forming processes, elaborates respectively on weathering degree in the bio-weathering layer of rocks and during the formation of soil material and clay, and evaluats the further tendency of weathering in the above-mentioned stages. The authors have discovered that the scales of weathering potential of the materials increase successively in the three stages, which indicates that the products in the above-mentioned three stages must have undergone stronger and stronger weathering in the primitive forming process of soil in Fildes Peninsula, Antarctic. But, because of relatively weak chemical weathering, it is reasonable that there are much more skeleton grains and little clay in primary soils in this region. Meanwhile, the authors have also verified that the weathering potential of crude rock determines to some extent decrease in the products' weathering potential in the different stages in primary soil-forming, thereby plays an important role in the genesis and development of the primary soil in the studied area. 展开更多
关键词 ANTARCTIC primary soil weathering potential
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