To investigate the role of macrophageinflammatory protein (MIP)-3αin human ovulation. Study of the levels of MIP-3αin serum and follicular fluid. The effects of interleukin (IL)-1α, IL-1 receptor antagonist (RA), a...To investigate the role of macrophageinflammatory protein (MIP)-3αin human ovulation. Study of the levels of MIP-3αin serum and follicular fluid. The effects of interleukin (IL)-1α, IL-1 receptor antagonist (RA), and tumor necrosis factor (TNF)-αon the secretion of MIP-3αby primary cultured granulosalutein cells and an immortalized granulosa cell line (GC1a) were investigated. Research laboratory at a university medical school. Fortysix patients with sterility undergoing in vitro fertilization and embryo transfer (IVFET).Follicular fluid was obtained from study participants, and granulosalutein cells and GC1a were incubated with IL-1α, IL-1RA, or TNF-αfor 4 to 32 hours. The concentration of MIP-3αin human follicular fluid was measured and correlated with oocyte maturation. We also cultured granulosa cells and examined the regulation of MIP-3αproduction. The concentrations of MIP-3αin the serum, follicular fluid, and culture medium were measured using enzymelinked immunoabsorbent assay. Concentrations of MIP-3αwere significantly higher in the follicular fluid, but it was not detected in the serum. Concentrations of MIP-3αwere statistically significantly higher in the follicular fluid containing mature oocytes than in follicular fluid containing immature oocytes. The production of MIP-3αwas markedly increased over the basal level after treatment with IL-1αand TNF-αin a dosedependent manner. The stimulatory effect of IL-1αwas inhibited by IL-1RA. Our data suggest that MIP-3αwas present in follicular fluid and correlated with oocyte maturation, and was regulated by IL-1αand TNF-α. Thus, MIP-3αmay play an important role in the human preovulatory process.展开更多
目的探讨促甲状腺激素(TSH)对多囊卵巢综合征(PCOS)患者代谢及生殖系统的影响。方法选取2015年4月-2017年4月在厦门市妇幼保健院生殖医学科行体外受精-胚胎移植(IVF-ET)治疗的PCOS患者146例,分为TSH≥2.5m U/L组52例、TSH<2.5 m U/L...目的探讨促甲状腺激素(TSH)对多囊卵巢综合征(PCOS)患者代谢及生殖系统的影响。方法选取2015年4月-2017年4月在厦门市妇幼保健院生殖医学科行体外受精-胚胎移植(IVF-ET)治疗的PCOS患者146例,分为TSH≥2.5m U/L组52例、TSH<2.5 m U/L组94例,检测TSH、性激素及代谢指标。记录两组获卵数、MⅡ卵数、可利用胚胎数及优质胚胎数。结果 TSH≥2.5 m U/L组甘油三酯(TG)、空腹胰岛素(FINS)、稳态模型(HOMA-IR)水平较TSH<2.5 m U/L组升高明显(P<0.05),TSH<2.5 m U/L组优质胚胎数、MⅡ卵子率较TSH≥2.5 m U/L组高,差异有统计学意义(P<0.05),TSH与TG、FINS、HOMA-IR呈正相关(P<0.05)。结论 TSH≥2.5 m U/L影响PCOS患者代谢及生殖系统。展开更多
文摘To investigate the role of macrophageinflammatory protein (MIP)-3αin human ovulation. Study of the levels of MIP-3αin serum and follicular fluid. The effects of interleukin (IL)-1α, IL-1 receptor antagonist (RA), and tumor necrosis factor (TNF)-αon the secretion of MIP-3αby primary cultured granulosalutein cells and an immortalized granulosa cell line (GC1a) were investigated. Research laboratory at a university medical school. Fortysix patients with sterility undergoing in vitro fertilization and embryo transfer (IVFET).Follicular fluid was obtained from study participants, and granulosalutein cells and GC1a were incubated with IL-1α, IL-1RA, or TNF-αfor 4 to 32 hours. The concentration of MIP-3αin human follicular fluid was measured and correlated with oocyte maturation. We also cultured granulosa cells and examined the regulation of MIP-3αproduction. The concentrations of MIP-3αin the serum, follicular fluid, and culture medium were measured using enzymelinked immunoabsorbent assay. Concentrations of MIP-3αwere significantly higher in the follicular fluid, but it was not detected in the serum. Concentrations of MIP-3αwere statistically significantly higher in the follicular fluid containing mature oocytes than in follicular fluid containing immature oocytes. The production of MIP-3αwas markedly increased over the basal level after treatment with IL-1αand TNF-αin a dosedependent manner. The stimulatory effect of IL-1αwas inhibited by IL-1RA. Our data suggest that MIP-3αwas present in follicular fluid and correlated with oocyte maturation, and was regulated by IL-1αand TNF-α. Thus, MIP-3αmay play an important role in the human preovulatory process.
文摘目的探讨促甲状腺激素(TSH)对多囊卵巢综合征(PCOS)患者代谢及生殖系统的影响。方法选取2015年4月-2017年4月在厦门市妇幼保健院生殖医学科行体外受精-胚胎移植(IVF-ET)治疗的PCOS患者146例,分为TSH≥2.5m U/L组52例、TSH<2.5 m U/L组94例,检测TSH、性激素及代谢指标。记录两组获卵数、MⅡ卵数、可利用胚胎数及优质胚胎数。结果 TSH≥2.5 m U/L组甘油三酯(TG)、空腹胰岛素(FINS)、稳态模型(HOMA-IR)水平较TSH<2.5 m U/L组升高明显(P<0.05),TSH<2.5 m U/L组优质胚胎数、MⅡ卵子率较TSH≥2.5 m U/L组高,差异有统计学意义(P<0.05),TSH与TG、FINS、HOMA-IR呈正相关(P<0.05)。结论 TSH≥2.5 m U/L影响PCOS患者代谢及生殖系统。
