目的观察经孕鼠尾静脉注射合体滋养细胞微粒(syncytiotrophoblast microparticles STBM)后,小鼠肾功能及肾脏病理改变。方法收集孕18 d C57小鼠的胎盘,制备小鼠合体滋养细胞微粒,利用电镜观察C57小鼠STBM的形态结构,BCA法测定C57小鼠STB...目的观察经孕鼠尾静脉注射合体滋养细胞微粒(syncytiotrophoblast microparticles STBM)后,小鼠肾功能及肾脏病理改变。方法收集孕18 d C57小鼠的胎盘,制备小鼠合体滋养细胞微粒,利用电镜观察C57小鼠STBM的形态结构,BCA法测定C57小鼠STBM的蛋白水平;将C57孕鼠分为STBM组、对照组和PBS组,STBM组:自C57小鼠孕8 d起,每天以蛋白浓度为0.15 mg/m L的STBM(0.2 m L)经鼠尾静脉回输至小鼠体内,至孕18 d时收集小鼠24 h尿液测定尿微量蛋白(M-TP)、尿肌酐(U-Crea)、尿蛋白肌酐比(M-TP/Cr)、尿酸(U-Uric)、24 h尿蛋白(24 h U-TP),采集全血测定血清尿素氮(BUN)、肌酐(Scr)、胱抑素C(Cys-C),取肾脏标本切片后行HE染色观察肾组织结构变化;对照组:正常孕18 d小鼠收集尿液、采集全血、取肾脏进行检测;PBS组:自孕8 d起每天经鼠尾静脉注射PBS 0.2 m L,余处理同STBM组。结果 STBM组孕鼠尿液M-TP、M-TP/Cr、24 h U-TP明显高于对照组和PBS组(P<0.01),与对照组比较,PBS组U-Uric未见明显变化(P>0.05),而STBM组U-Uric显著降低(P<0.01);STBM组血清BUN、Scr、Cys-C含量显著升高(P<0.01);STBM组肾脏HE染色可见肾小球内皮细胞增生、肿胀、变形,肾小管云雾状变性。结论类子痫前期模型鼠有肾损伤,STBM可能在子痫前期肾损害中起重要作用。展开更多
Objective: The purpose of this study was to determine syncytiotrophoblast intercellular adhesion molecule- 1 expression in villitis and in normal chorionic villi from term (37- 42 weeks of gestation) placentas with or...Objective: The purpose of this study was to determine syncytiotrophoblast intercellular adhesion molecule- 1 expression in villitis and in normal chorionic villi from term (37- 42 weeks of gestation) placentas with or without villitis. Study design: A cross-sectional study was conducted to determine syncytiotrophoblast intercellular adhesion molecule- 1 expression in villitis (n = 16) and in normal villi from placentas with or without villitis (n = 16). Villitis was diagnosed with antibodies to human leukocyte antigen- DR and CD3 and hematoxylin and eosin staining of serial sections; intercellular adhesion molecule- 1 reactivity in syncytiotrophoblast was confirmed with antibodies to intercellular adhesion molecule- 1 and cytokeratin. Results: Villitis lesions had higher syncytiotrophoblast intercellular adhesion molecule- 1 expression than normal chorionic villi from placentas with (19.9% vs 3.5% villi; P < .001) or without (19.9% vs 0.31% villi; P < .001) villitis. Normal villi from placentas with villitis had higher syncytiotrophoblast intercellular adhesion molecule- 1 than villi from placentas without villitis (3.5% vs 0.31% villi; P <.001). Conclusion: Placentas with villitis have significantly more syncytiotrophoblast intercellular adhesion molecule- 1 expression than placentas without villitis. The finding that normal villi from placentas with villitis have more syncytiotrophoblast intercellular adhesion molecule1 than normal villi from placentas without villitis suggests that syncytiotrophoblast intercellular adhesion molecule- 1 could be the first step in villitis development.展开更多
文摘目的观察经孕鼠尾静脉注射合体滋养细胞微粒(syncytiotrophoblast microparticles STBM)后,小鼠肾功能及肾脏病理改变。方法收集孕18 d C57小鼠的胎盘,制备小鼠合体滋养细胞微粒,利用电镜观察C57小鼠STBM的形态结构,BCA法测定C57小鼠STBM的蛋白水平;将C57孕鼠分为STBM组、对照组和PBS组,STBM组:自C57小鼠孕8 d起,每天以蛋白浓度为0.15 mg/m L的STBM(0.2 m L)经鼠尾静脉回输至小鼠体内,至孕18 d时收集小鼠24 h尿液测定尿微量蛋白(M-TP)、尿肌酐(U-Crea)、尿蛋白肌酐比(M-TP/Cr)、尿酸(U-Uric)、24 h尿蛋白(24 h U-TP),采集全血测定血清尿素氮(BUN)、肌酐(Scr)、胱抑素C(Cys-C),取肾脏标本切片后行HE染色观察肾组织结构变化;对照组:正常孕18 d小鼠收集尿液、采集全血、取肾脏进行检测;PBS组:自孕8 d起每天经鼠尾静脉注射PBS 0.2 m L,余处理同STBM组。结果 STBM组孕鼠尿液M-TP、M-TP/Cr、24 h U-TP明显高于对照组和PBS组(P<0.01),与对照组比较,PBS组U-Uric未见明显变化(P>0.05),而STBM组U-Uric显著降低(P<0.01);STBM组血清BUN、Scr、Cys-C含量显著升高(P<0.01);STBM组肾脏HE染色可见肾小球内皮细胞增生、肿胀、变形,肾小管云雾状变性。结论类子痫前期模型鼠有肾损伤,STBM可能在子痫前期肾损害中起重要作用。
文摘Objective: The purpose of this study was to determine syncytiotrophoblast intercellular adhesion molecule- 1 expression in villitis and in normal chorionic villi from term (37- 42 weeks of gestation) placentas with or without villitis. Study design: A cross-sectional study was conducted to determine syncytiotrophoblast intercellular adhesion molecule- 1 expression in villitis (n = 16) and in normal villi from placentas with or without villitis (n = 16). Villitis was diagnosed with antibodies to human leukocyte antigen- DR and CD3 and hematoxylin and eosin staining of serial sections; intercellular adhesion molecule- 1 reactivity in syncytiotrophoblast was confirmed with antibodies to intercellular adhesion molecule- 1 and cytokeratin. Results: Villitis lesions had higher syncytiotrophoblast intercellular adhesion molecule- 1 expression than normal chorionic villi from placentas with (19.9% vs 3.5% villi; P < .001) or without (19.9% vs 0.31% villi; P < .001) villitis. Normal villi from placentas with villitis had higher syncytiotrophoblast intercellular adhesion molecule- 1 than villi from placentas without villitis (3.5% vs 0.31% villi; P <.001). Conclusion: Placentas with villitis have significantly more syncytiotrophoblast intercellular adhesion molecule- 1 expression than placentas without villitis. The finding that normal villi from placentas with villitis have more syncytiotrophoblast intercellular adhesion molecule1 than normal villi from placentas without villitis suggests that syncytiotrophoblast intercellular adhesion molecule- 1 could be the first step in villitis development.
