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基因组设计与合成:从复写到理性设计 被引量:2
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作者 汪君仪 武晓乐 +1 位作者 曹月阳 李炳志 《合成生物学》 CSCD 2021年第2期247-255,共9页
基因组合成相关技术的进步推动人工基因组合成能力不断取得突破,合成基因组学成为了近年来的研究热点,逐步完成了病毒、原核生物基因组的全合成,真核生物基因组设计合成也取得了阶段性突破。在基因组合成的研究过程中,基因组设计的原则... 基因组合成相关技术的进步推动人工基因组合成能力不断取得突破,合成基因组学成为了近年来的研究热点,逐步完成了病毒、原核生物基因组的全合成,真核生物基因组设计合成也取得了阶段性突破。在基因组合成的研究过程中,基因组设计的原则不断拓展,基因组设计的尺度由病毒及噬菌体基因组成功复写发展到蕈状支原体JCVI-syn3.0基因组大幅度简化,在真核生物酿酒酵母基因组合成中探索多种基因组设计原则。本文主要综述了人工基因组设计的相关进展,主要内容包括:(1)人工基因组密码子的改变:外源基因密码子的优化,密码子丰度转变原则的探究,大肠杆菌中密码子的删除;(2)人工标签的添加及应用:利用同义密码子替换在合成型基因组中添加标签以区分合成型与野生型基因组;(3)人工位点的添加:酿酒酵母合成型染色体重组系统的开发,优化及应用;(4)基因组简化方面的研究;(5)对基因组理性设计、基因组简化规律挖掘等进行了展望。 展开更多
关键词 合成基因组 基因组设计 密码子删除 合成型标签 SCRAMBLE 基因组简化
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基因组的设计与工程化构建 被引量:1
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作者 薛小莉 覃重军 《中国科学院院刊》 CSCD 北大核心 2018年第11期1205-1210,共6页
合成生物学是利用工程化的思想来设计和构建新的生物基因组,是近年来的研究热点。近年来,对原核细胞支原体天然小基因组进行从头合成并进一步设计与构建最小基因组,对原核模式生物大肠杆菌基因组的不断删减及全基因组密码子简约化设计... 合成生物学是利用工程化的思想来设计和构建新的生物基因组,是近年来的研究热点。近年来,对原核细胞支原体天然小基因组进行从头合成并进一步设计与构建最小基因组,对原核模式生物大肠杆菌基因组的不断删减及全基因组密码子简约化设计与人工合成测试,以及对真核模式生物酿酒酵母基因组人工设计与合成测试都取得了很大成功,极大地促进了我们对生命的理解。我国的基因组的设计与工程化构建方面的研究虽然起步较晚,但近年来取得了国际瞩目的成果。基因组的设计与构建为深入了解生命起源与进化,并为进一步构建具有强大应用功能的新型生命体奠定了基础。 展开更多
关键词 基因组设计 基因组删减 基因组合成 工程化构建
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合成生物学重要研究方向进展 被引量:34
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作者 丁明珠 李炳志 +3 位作者 王颖 谢泽雄 刘夺 元英进 《合成生物学》 2020年第1期7-28,共22页
合成生物学作为一个新兴的交叉学科领域,随着DNA合成技术的进步和合成生物学理念的深入,多个研究方向取得了长足发展。本文主要对基因回路、基因组设计合成、细胞工厂和人工多细胞体系的进展进行了综述。可设计构建的人工基因线路的复... 合成生物学作为一个新兴的交叉学科领域,随着DNA合成技术的进步和合成生物学理念的深入,多个研究方向取得了长足发展。本文主要对基因回路、基因组设计合成、细胞工厂和人工多细胞体系的进展进行了综述。可设计构建的人工基因线路的复杂度逐步提升,人工控制更加精细;组装技术取得快速进展的同时,人工基因组的设计深度也在不断拓展,设计合成的人工基因组由支原体拓展向大肠杆菌,甚至真核生物酿酒酵母,推动了生物进化演化的研究;细胞工厂的设计构建在逐步挑战代谢途径更长、复杂程度更高的化合物的合成,模块化和正交化策略对复杂细胞工厂构建的支撑作用日益明显,鲁棒性和适配性成为细胞工厂构建需要考虑的重要问题;人工多细胞体系的设计构建已经从设计构建两菌体系向多菌体系扩展,通过多种原则进行设计,实现更加复杂的预期功能。本文也对合成生物学与其他学科交叉融合产生的一些新研究方向进行了简介。 展开更多
关键词 合成生物学 基因回路 基因组设计合成 细胞工厂 人工多细胞体系
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Inheritance of Main Morphological Traits and Chemical Parameters' Analysis of Oriental Tobacco
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作者 Vesselina Masheva Maria Kasheva 《Journal of Agricultural Science and Technology(A)》 2016年第6期417-422,共6页
This study was conducted to evaluate four different parental oriental varieties with their F1 crosses to estimate inheritance of some morphological traits, such as plant hight, number of leaves, total dry weigh and ch... This study was conducted to evaluate four different parental oriental varieties with their F1 crosses to estimate inheritance of some morphological traits, such as plant hight, number of leaves, total dry weigh and chemical compounds, especially nicotine and sugars during sun-curing period. The oriental varieties Kozarsko 541, Plovdiv 50 and Krumovgrad 17 from Bulgaria and Basma Xanthi 101 from Greece and their respective crosses with Basma Xanthi 101 were studied. The study was set up in 2014 and 2015 in experimental field of the Tobacco and Tobacco Products Institute, Plovdiv, using randomized block design with four replications. Common agricultural practices were applied during the growing season. The result indicated that in hybrid combination with parental varieties Kozarsko 541 and Plovdiv 50, except total dry weight, additive gene effects are important for traits height of plant and number of leaves. Height of plant and number of leaves are inherited partially dominant to parent with higher value and total dry weight inheritance was over dominant. In hybrid combination Krumovgrad 17 x Basma Xanthi 101, inheritance of the plant height and leaves number were over dominant type. Total dry weight is inherited partially dominant. In a word, prevailing mode for inheritance of plant hight and total dry weight was the over dominance and for number of leaves it was partial dominance. In sun-curing with yellowing stage, nicotine decreased and sugars increased in all tested varieties with a highest percentage in variety Kozarsko 541 (nicotine) and Krumovgrad 17 (sugars). 展开更多
关键词 Oriental tobacco inhedtanee character morphological traits chemical parameters.
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Stability Assessment of Some West African Okra (Abelmoschus caillel~ Genotypes in Nigerian Genebank
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作者 Aladele Sunday Ezekiel Omolayo Johnson Ariyo Olusola Babatunde Kehinde2 《Journal of Life Sciences》 2011年第11期906-912,共7页
Stability among 50 accessions of West African okra (Abelmoschus caillei) was assessed under three diverse ecological environments at Abeokuta, Ibadan and Mokwa in Nigeria during 2005 and 2006 cropping season. The ac... Stability among 50 accessions of West African okra (Abelmoschus caillei) was assessed under three diverse ecological environments at Abeokuta, Ibadan and Mokwa in Nigeria during 2005 and 2006 cropping season. The accessions were grown in a Randomized Complete Block Design with three replications; data were collected on 5-10 randomly selected plants from each plot. Only 20 accessions were subjected to stability analysis on the basis of yield across the three environments. The joint regression analysis, deviation means square were computed using Eberhart and Russell method and complemented with Francis and Kannenberg method. The regression coefficients of accessions mean yields on the environmental index resulted in regression coefficients ranging in values from 0.5549 to 1.6667. OAA/96/175-5328, NGAE-96-011 and NGAE-96-0060 were among the superior genotypes with high yield performance. The large variation in regression values indicated large differences in genotype response to different environments. It suggests that stability concept of Ebelhart and Russell could be modified to use any yield components that has strong correlation with yield for stability analysis. The two promising accessions ofA. caillei (NGAE-96-011 and NGAE-96-0060) needed to be further tested on farmers' field to obtain on-farm data, alter which it should be recommended for official registration and released by the National Committee on Naming, Registration and Release of Crop varieties in Nigeria. 展开更多
关键词 Stability assessment environmental index GENOTYPE West African okra.
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Genotype and Environmental Effects on Cadmium Concentration in Maize
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作者 Vlado Kovacevic Antun Vragolovic 《Journal of Life Sciences》 2011年第11期926-932,共7页
Seven parents and their 21 Fl diallel crosses of maize were grown under field conditions in the 2001 and 2002 growing seasons on two soils mutually air-distanced 800 m (BI = fluvisol and B2 = stagnic albeluvisol; pH ... Seven parents and their 21 Fl diallel crosses of maize were grown under field conditions in the 2001 and 2002 growing seasons on two soils mutually air-distanced 800 m (BI = fluvisol and B2 = stagnic albeluvisol; pH in KCI = 7.02 and 3.85, humus = 2.75 and 2.02, CaCO3 = 11.4% and 0, for B 1 and B2, respectively). Also, the B 1 and B2 were differently in NH4Acetate-EDTA soluble fraction (mg.kgl: calcium 25,500 and 2,300; zinc 1.61 and 0.53; cadmium 0.195 and 0.064, respectively). The experiments were conducted in four replicates (the basic plot 16.8 m2) in a randomized complete block design. The ear-leaf was taken at the beginning of silking and grain samples at maturity. Mean leaf-Cd (mg Cd.kg-t in dry matter) were 0.102 and 0.072, for 2001 and 2002; 0.064 and 0.195, for BI and B2, while differences in level of genotype were from 0.040 to 0.581 (the parents) and from 0.046 to 0.171 (the hybrids). Six hybrids of Bc707-1 had considerably higher leaf-Cd in comparison with six hybrids of Bc265-1 (means 0.100 and 0.050 respectively). Grain-Cd was under detectable range (〈 0.040) with exception of Bc707-1 parent on the B2 soil (0.17 mg Cd-kg^-1). 展开更多
关键词 CADMIUM GENOTYPE grain leaf MAIZE soil effects year effects
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人工合成生命的研究发展概述 被引量:1
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作者 李会珍 翟心慧 《生物学通报》 CAS 2020年第10期5-7,共3页
近年来,人工合成生命领域取得了里程碑式的进展,其中世界首例单条染色体酿酒酵母的诞生引起了全球的关注,成为热点话题。《普通高中生物学课程标准(2017年版)》中建议学校针对“社会热点中的生物学问题”开设校本课程。梳理与介绍了人... 近年来,人工合成生命领域取得了里程碑式的进展,其中世界首例单条染色体酿酒酵母的诞生引起了全球的关注,成为热点话题。《普通高中生物学课程标准(2017年版)》中建议学校针对“社会热点中的生物学问题”开设校本课程。梳理与介绍了人工合成生命领域的研究发展、应用及安全伦理问题,旨在为中学生物学教师提供一定的教学参考。 展开更多
关键词 高中生物学 人工合成生命 合成生物学 细胞工程 基因组设计
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而今迈步从头越:马铃薯育种跨入“有种”时代 被引量:3
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作者 许操 《植物学报》 CAS CSCD 北大核心 2021年第5期516-519,共4页
现代栽培马铃薯(Solanumtuberosum)是同源四倍体,基因组高度杂合,遗传组成复杂,存在严重的自交衰退,优异性状难以聚合,使得育种周期漫长,造成基于种子传代的马铃薯杂交育种难以突破。与此同时,块茎无性繁殖导致马铃薯繁殖系数低、储运... 现代栽培马铃薯(Solanumtuberosum)是同源四倍体,基因组高度杂合,遗传组成复杂,存在严重的自交衰退,优异性状难以聚合,使得育种周期漫长,造成基于种子传代的马铃薯杂交育种难以突破。与此同时,块茎无性繁殖导致马铃薯繁殖系数低、储运成本高和易携带病虫害等痼疾,且种薯脱毒成本高,限制其产业化发展。近期,中国农业科学院深圳农业基因组研究所黄三文团队运用基因组设计理论和方法体系培育杂交马铃薯,用二倍体育种替代四倍体育种,实现了用杂交种子繁殖替代薯块繁殖,创制了第1代高纯度的二倍体自交系和杂种优势明显的杂交马铃薯品系。该研究是马铃薯育种领域里程碑式的重大成果,开启了基于基因组设计和种子迭代的马铃薯生物育种新纪元。 展开更多
关键词 马铃薯 基因组设计 自交系 杂交育种
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