Objective: To retrospectively analyze the clinical data of 275 cases of cervical cancer (CC) in our hospital, and investigate the clinicopathological parameters of cervical cancer based on the expression of p16INK4A p...Objective: To retrospectively analyze the clinical data of 275 cases of cervical cancer (CC) in our hospital, and investigate the clinicopathological parameters of cervical cancer based on the expression of p16INK4A proteins. Methods: The clinical information of 275 patients with cervical cancer were retrospectively analyzed between 2006 and 2011, including the patients' age, clinical FIGO stage, differentiation, histologic grade, infiltration depth, treatment, pathological diagnosis after surgery, and results of following-up.Immunohistochemistry was also done on sections of confirmed cancer specimens without prior chemotherapy/radiotherapy. Results: Among various clinicopathological parameters, the median age was 48 years old. The grade was significantly associated with histological type, HPV infection and with lymph node invasion. FIGO stage was strongly correlated to the infiltration depth and lymph node metastasis. P16INK4A expression was significantly correlated with histologic grade. However, there were no differences between p16INK4A staining and patient's age, histopathology and lymph node metastases. Conclusion: The incidence of cervical cancer becomes increasingly younger. Additionally, p16INK4A can function as a diagnostic marker of cervical carcinomas.展开更多
Among the human users of the Internet of Things,the hearing-impaired is a special group of people for whom normal information expression forms,such as voice and video are unaccessible,and most of them have some diffic...Among the human users of the Internet of Things,the hearing-impaired is a special group of people for whom normal information expression forms,such as voice and video are unaccessible,and most of them have some difficulty in understanding information in text form.The hearing-impaired are accustomed to receiving information expressed in sign language.For this situation,a new information expression form for the Internet of Things oriented toward the hearing-impaired is proposed in this paper,and the new expression is based on sign language video synthesis.Under the sign synthesis frame,three modules are necessary:constructing database,searching for appropriate sign language video units and transition units,and generating interpolated frames.With this method,text information could be transformed into sign language expression for the hearing-impaired.展开更多
Safe and efficient gene transfer systems are the basis of gene therapy applications. Non-integrating lentiviral(NIL) vectors are among the most promising candidates for gene transfer tools, because they exhibit high t...Safe and efficient gene transfer systems are the basis of gene therapy applications. Non-integrating lentiviral(NIL) vectors are among the most promising candidates for gene transfer tools, because they exhibit high transfer efficiency in both dividing and non-dividing cells and do not present a risk of insertional mutagenesis. However, non-integrating lentiviral vectors cannot introduce stable exogenous gene expression to dividing cells, thereby limiting their application. Here, we report the design of a non-integrating lentiviral vector that contains the minimal scaffold/matrix attachment region(S/MAR) sequence(SNIL), and this SNIL vector is able to retain episomal transgene expression in dividing cells. Using SNIL vectors, we detected the expression of the eGFP gene for 61 days in SNIL-transduced stable CHO cells, either with selection or not. In the NIL group without the S/MAR sequence, however, the transduced cells died under selection for the transient expression of NIL vectors. Furthermore,Southern blot assays demonstrated that the SNIL vectors were retained extrachromosomally in the CHO cells. In conclusion,the minimal S/MAR sequence retained the non-integrating lentiviral vectors in dividing cells, which indicates that SNIL vectors have the potential for use as a gene transfer tool.展开更多
The periodic or quasi-periodic orbits around collinear Lagrange points present many properties that are advantageous for space missions. These Lagrange point orbits are exponentially unstable. On the basis of an analy...The periodic or quasi-periodic orbits around collinear Lagrange points present many properties that are advantageous for space missions. These Lagrange point orbits are exponentially unstable. On the basis of an analytical method, an orbit control strategy that is designed to eliminate the dominant unstable components of Lagrange point orbits is developed. The proposed strategy enables the derivation of the analytical expression of nonlinear control force. The control parameter of this strategy can be arbitrarily selected provided that the parameter is considerably lower than the negative eigenvalue of motion equations, and that the energy required keeps the same order of magnitude. The periodic or quasi-periodic orbit of controlled equations remains near the periodic or quasi-periodic orbit of uncontrolled equations.展开更多
基金Supported by Research Project for Traditional Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine (jc87)Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT: 1171)
文摘Objective: To retrospectively analyze the clinical data of 275 cases of cervical cancer (CC) in our hospital, and investigate the clinicopathological parameters of cervical cancer based on the expression of p16INK4A proteins. Methods: The clinical information of 275 patients with cervical cancer were retrospectively analyzed between 2006 and 2011, including the patients' age, clinical FIGO stage, differentiation, histologic grade, infiltration depth, treatment, pathological diagnosis after surgery, and results of following-up.Immunohistochemistry was also done on sections of confirmed cancer specimens without prior chemotherapy/radiotherapy. Results: Among various clinicopathological parameters, the median age was 48 years old. The grade was significantly associated with histological type, HPV infection and with lymph node invasion. FIGO stage was strongly correlated to the infiltration depth and lymph node metastasis. P16INK4A expression was significantly correlated with histologic grade. However, there were no differences between p16INK4A staining and patient's age, histopathology and lymph node metastases. Conclusion: The incidence of cervical cancer becomes increasingly younger. Additionally, p16INK4A can function as a diagnostic marker of cervical carcinomas.
基金supported by the National Natural Science Foundation of China(Nos.60825203,60973056,60973057,U0935004)National Technology Support Project(2007BAH13B01)+2 种基金Beijing Municipal Natural Science Foundation(4102009)Scientific Research Common Program of Beijing Municipal Commission of Education(KM200710005023)PHR(IHLB)
文摘Among the human users of the Internet of Things,the hearing-impaired is a special group of people for whom normal information expression forms,such as voice and video are unaccessible,and most of them have some difficulty in understanding information in text form.The hearing-impaired are accustomed to receiving information expressed in sign language.For this situation,a new information expression form for the Internet of Things oriented toward the hearing-impaired is proposed in this paper,and the new expression is based on sign language video synthesis.Under the sign synthesis frame,three modules are necessary:constructing database,searching for appropriate sign language video units and transition units,and generating interpolated frames.With this method,text information could be transformed into sign language expression for the hearing-impaired.
基金supported by the Major State Basic Research Development Program of China(2011CB965203)
文摘Safe and efficient gene transfer systems are the basis of gene therapy applications. Non-integrating lentiviral(NIL) vectors are among the most promising candidates for gene transfer tools, because they exhibit high transfer efficiency in both dividing and non-dividing cells and do not present a risk of insertional mutagenesis. However, non-integrating lentiviral vectors cannot introduce stable exogenous gene expression to dividing cells, thereby limiting their application. Here, we report the design of a non-integrating lentiviral vector that contains the minimal scaffold/matrix attachment region(S/MAR) sequence(SNIL), and this SNIL vector is able to retain episomal transgene expression in dividing cells. Using SNIL vectors, we detected the expression of the eGFP gene for 61 days in SNIL-transduced stable CHO cells, either with selection or not. In the NIL group without the S/MAR sequence, however, the transduced cells died under selection for the transient expression of NIL vectors. Furthermore,Southern blot assays demonstrated that the SNIL vectors were retained extrachromosomally in the CHO cells. In conclusion,the minimal S/MAR sequence retained the non-integrating lentiviral vectors in dividing cells, which indicates that SNIL vectors have the potential for use as a gene transfer tool.
基金supported by the National Natural Science Foundation of China (Grant Nos. 10832004 and 11102006)the Fan-Zhou Foundation (Grant No. 20110502)
文摘The periodic or quasi-periodic orbits around collinear Lagrange points present many properties that are advantageous for space missions. These Lagrange point orbits are exponentially unstable. On the basis of an analytical method, an orbit control strategy that is designed to eliminate the dominant unstable components of Lagrange point orbits is developed. The proposed strategy enables the derivation of the analytical expression of nonlinear control force. The control parameter of this strategy can be arbitrarily selected provided that the parameter is considerably lower than the negative eigenvalue of motion equations, and that the energy required keeps the same order of magnitude. The periodic or quasi-periodic orbit of controlled equations remains near the periodic or quasi-periodic orbit of uncontrolled equations.
