The aim of the research was to discuss the genetic relationships between Piper methysticum, Pepper and other wild species in Pepper genus. DNA was extracted from leaves which belonged to 28 germplasms including 6 mate...The aim of the research was to discuss the genetic relationships between Piper methysticum, Pepper and other wild species in Pepper genus. DNA was extracted from leaves which belonged to 28 germplasms including 6 materials of P. methysticum, 21 maerials of cultivated and wild Pepper, 1 material of Peperomia pellucida belonged to different genus. Premiers with good band-type and high polymorphism and resolution were selected from 64 pairs of primers for AFLP amplification and the clustering analysis was conducted with MVSP3.13f software. 191 bands were amplified by 4 pairs of premiers, 189 of which had polymorphism, being 98.6%. 28 germplasms were classified into 6 different groups at the genetic similarity coefficient of 0.52 by silver staining AFLP, in which 6 materials of Piper methysticum were clustered into a single group, indicating that P. methysticum belonged to Pepper family of Pepper genus but were distantly related to the others. The research provided the basis for selecting rootstocks for P. methysticum graft, molecular identification of P. methysticum and the fingerprint construction of P. methysticum.展开更多
Unmanned aerial vehicle(UAV)was introduced to take road segment traffic surveillance.Considering the limited UAV maximum flight distance,UAV route planning problem was studied.First,a multi-objective optimization mode...Unmanned aerial vehicle(UAV)was introduced to take road segment traffic surveillance.Considering the limited UAV maximum flight distance,UAV route planning problem was studied.First,a multi-objective optimization model of planning UAV route for road segment surveillance was proposed,which aimed to minimize UAV cruise distance and minimize the number of UAVs used.Then,an evolutionary algorithm based on Pareto optimality technique was proposed to solve multi-objective UAV route planning problem.At last,a UAV flight experiment was conducted to test UAV route planning effect,and a case with three scenarios was studied to analyze the impact of different road segment lengths on UAV route planning.The case results show that the optimized cruise distance and the number of UAVs used decrease by an average of 38.43% and 33.33%,respectively.Additionally,shortening or extending the length of road segments has different impacts on UAV route planning.展开更多
The genetic diversity of 27 different Cervus nippon hortulorum was studied to provide theoretical evidence for their identification and utilization. The genomie DNA of 27 different Cereus nippon hortulorum were analyz...The genetic diversity of 27 different Cervus nippon hortulorum was studied to provide theoretical evidence for their identification and utilization. The genomie DNA of 27 different Cereus nippon hortulorum were analyzed by amplified fragment length polymorphism （AFLP）. 11,443 bands associated with genetic polymorphism among total 15,169 bands were obtained with 9 kinds of primer pairs and restriction endonuclease EcoRI/Msel, percentage of polymorphie band was 78.43%, 1,271 polymorphic locus were shown per primer pair. The AFLP data showed that average genetic similarity was 0.7841 （0.6809-0.8648）. 27 samples were classified into Group I and Group II with cluster analysis, and Group II was divided into five subgroups. The result of AFLP and cluster analysis concluded that there was high genetic variation, which associated with orientated artificial breed selection and breeding in the population. Genetic similarity of Group II-4 was the highest, more than 0.82, while genetic distance in this group was the shortest, from 0.1354 to 0.1563, which was coordinated with breeding record. All these showed that there was great genetic polymorphism among the deer population. The results laid the foundation for main quantitative trait locus （QTLs） of Cervus nippon hortulorum.展开更多
Mutagenesis of Lactobacillus casei subsp, rhamnosus Xl-12 after low power microwave irradiation was investigated. Under a microwave power of 400 W and irradiation length of 3 min, a mutated strain W4-3-9 with high-yie...Mutagenesis of Lactobacillus casei subsp, rhamnosus Xl-12 after low power microwave irradiation was investigated. Under a microwave power of 400 W and irradiation length of 3 min, a mutated strain W4-3-9 with high-yield L-lactic acid was obtained by screening. Compared with the starting strain X1-12, the L-lactic acid production of W4-3-9 was increased by 58.0% at a concentration of 115.8 g/L. The strain maintained the capability of producing a high L-lactic acid level after 10 generations. Cell surface morphology and DNA structures of parental and mutated strains were observed by atomic force microscopy （ AFM ）. Amplified fragment length polymorphism （AFLP） analysis suggested the difference in AFLP band pattern between the mutated and non-mutated strains. Sequencing and BLAST analysis revealed that the catalytic site of lactate dehydrogenase （DHL） was changed due to the microwave induced mutation.展开更多
Haliotis diversicolor Reeve is one of the most important mollusks cultured in South China. Preliminary genetic linkage maps were constructed with amplified fragment length polymorphism (AFLP) markers. A total of 2 596...Haliotis diversicolor Reeve is one of the most important mollusks cultured in South China. Preliminary genetic linkage maps were constructed with amplified fragment length polymorphism (AFLP) markers. A total of 2 596 AFLP markers were obtained from 28 primer combinations in two parents and 78 offsprings. Among them, 412 markers (15.9%) were polymorphic and segregated in the mapping family. Chi-square tests showed that 151 (84.4%) markers segregated according to the expected 1:1 Mendelian ratio (P<0.05) in the female parent, and 200 (85.8%) in the male parent. For the female map, 179 markers were used for linkage analysis and 90 markers were assigned to 17 linkage groups with an average interval length of 25.7 cm. For the male map, 233 markers were used and 94 were mapped into 18 linkage groups, with an average interval of 25.0 cm. The estimated genome length was 2 773.0 cm for the female and 2 817.1 cm for the male map. The observed length of the linkage map was 1 875.2 cm and 1 896.5 cm for the female and male maps, respectively. When doublets were considered, the map length increased to 2 152.8 cm for the female and 2 032.7 cm for the male map, corresponding to genome coverage of 77.6% and 72.2%, respectively.展开更多
Periodic composites with band gaps that prevent the propagation of elastic waves in certain frequency ranges can be used to control waves for a variety of engineering applications. Although studies on the characterist...Periodic composites with band gaps that prevent the propagation of elastic waves in certain frequency ranges can be used to control waves for a variety of engineering applications. Although studies on the characteristics of these materials, which are called phononic crystals (PCs), have yielded a large number of positive results in recent years, there is still a lack of effective design methods. In this work, a multi-objective optimization approach based on the band gap mechanism and an intelligent algorithm is used to design a one-dimensional (1D) slab construction of PCs. The design aims to fit pre-determined bands by arranging the available materials properly. Obtained by analyzing the wave transmission in periodic layers, the objective functions are linked to the optimization program to obtain a proper solution set. The results of the numerical simulations demonstrate that without constructing complicated structures, the design method is able to produce PCs that overcome the limitations of two-component PCs and hence can feasibly and effectively achieve the design targets. The design approach presented in this paper can be extended to two-or three-dimensional systems and has great potential for the development of sound/ultrasound isolation structures, multiple band frequency filters, and other applications.展开更多
基金Supported by the National Key Project of Tenth-five Year Plan(2001BA707B)School Foundation Program of Henan Science and Technology University~~
文摘The aim of the research was to discuss the genetic relationships between Piper methysticum, Pepper and other wild species in Pepper genus. DNA was extracted from leaves which belonged to 28 germplasms including 6 materials of P. methysticum, 21 maerials of cultivated and wild Pepper, 1 material of Peperomia pellucida belonged to different genus. Premiers with good band-type and high polymorphism and resolution were selected from 64 pairs of primers for AFLP amplification and the clustering analysis was conducted with MVSP3.13f software. 191 bands were amplified by 4 pairs of premiers, 189 of which had polymorphism, being 98.6%. 28 germplasms were classified into 6 different groups at the genetic similarity coefficient of 0.52 by silver staining AFLP, in which 6 materials of Piper methysticum were clustered into a single group, indicating that P. methysticum belonged to Pepper family of Pepper genus but were distantly related to the others. The research provided the basis for selecting rootstocks for P. methysticum graft, molecular identification of P. methysticum and the fingerprint construction of P. methysticum.
基金Project(2009AA11Z220)supported by National High Technology Research and Development Program of ChinaProjects(61070112,61070116)supported by the National Natural Science Foundation of China+1 种基金Project(2012LLYJTJSJ077)supported by the Ministry of Public Security of ChinaProject(KYQD14003)supported by Tianjin University of Technology and Education,China
文摘Unmanned aerial vehicle(UAV)was introduced to take road segment traffic surveillance.Considering the limited UAV maximum flight distance,UAV route planning problem was studied.First,a multi-objective optimization model of planning UAV route for road segment surveillance was proposed,which aimed to minimize UAV cruise distance and minimize the number of UAVs used.Then,an evolutionary algorithm based on Pareto optimality technique was proposed to solve multi-objective UAV route planning problem.At last,a UAV flight experiment was conducted to test UAV route planning effect,and a case with three scenarios was studied to analyze the impact of different road segment lengths on UAV route planning.The case results show that the optimized cruise distance and the number of UAVs used decrease by an average of 38.43% and 33.33%,respectively.Additionally,shortening or extending the length of road segments has different impacts on UAV route planning.
文摘The genetic diversity of 27 different Cervus nippon hortulorum was studied to provide theoretical evidence for their identification and utilization. The genomie DNA of 27 different Cereus nippon hortulorum were analyzed by amplified fragment length polymorphism （AFLP）. 11,443 bands associated with genetic polymorphism among total 15,169 bands were obtained with 9 kinds of primer pairs and restriction endonuclease EcoRI/Msel, percentage of polymorphie band was 78.43%, 1,271 polymorphic locus were shown per primer pair. The AFLP data showed that average genetic similarity was 0.7841 （0.6809-0.8648）. 27 samples were classified into Group I and Group II with cluster analysis, and Group II was divided into five subgroups. The result of AFLP and cluster analysis concluded that there was high genetic variation, which associated with orientated artificial breed selection and breeding in the population. Genetic similarity of Group II-4 was the highest, more than 0.82, while genetic distance in this group was the shortest, from 0.1354 to 0.1563, which was coordinated with breeding record. All these showed that there was great genetic polymorphism among the deer population. The results laid the foundation for main quantitative trait locus （QTLs） of Cervus nippon hortulorum.
基金Sponsored by the National Natural Science Foundation of China(Grant No.50778053)
文摘Mutagenesis of Lactobacillus casei subsp, rhamnosus Xl-12 after low power microwave irradiation was investigated. Under a microwave power of 400 W and irradiation length of 3 min, a mutated strain W4-3-9 with high-yield L-lactic acid was obtained by screening. Compared with the starting strain X1-12, the L-lactic acid production of W4-3-9 was increased by 58.0% at a concentration of 115.8 g/L. The strain maintained the capability of producing a high L-lactic acid level after 10 generations. Cell surface morphology and DNA structures of parental and mutated strains were observed by atomic force microscopy （ AFM ）. Amplified fragment length polymorphism （AFLP） analysis suggested the difference in AFLP band pattern between the mutated and non-mutated strains. Sequencing and BLAST analysis revealed that the catalytic site of lactate dehydrogenase （DHL） was changed due to the microwave induced mutation.
基金Supported by the National Basic Research Program of China (973 Program) (Nos. 2010CB126405 and 2009CB126005)the Key Project for International Science and Technology Cooperation, Ministry of Science and Technology of China (No. 2004DFA07200)+2 种基金the Great Science and Technique Program of Hainan Province (No. 06120)the Hainan Key Laboratory of Tropical Hydrobiological Technology (No. shkyjj0810)supported by the Rutgers University
文摘Haliotis diversicolor Reeve is one of the most important mollusks cultured in South China. Preliminary genetic linkage maps were constructed with amplified fragment length polymorphism (AFLP) markers. A total of 2 596 AFLP markers were obtained from 28 primer combinations in two parents and 78 offsprings. Among them, 412 markers (15.9%) were polymorphic and segregated in the mapping family. Chi-square tests showed that 151 (84.4%) markers segregated according to the expected 1:1 Mendelian ratio (P<0.05) in the female parent, and 200 (85.8%) in the male parent. For the female map, 179 markers were used for linkage analysis and 90 markers were assigned to 17 linkage groups with an average interval length of 25.7 cm. For the male map, 233 markers were used and 94 were mapped into 18 linkage groups, with an average interval of 25.0 cm. The estimated genome length was 2 773.0 cm for the female and 2 817.1 cm for the male map. The observed length of the linkage map was 1 875.2 cm and 1 896.5 cm for the female and male maps, respectively. When doublets were considered, the map length increased to 2 152.8 cm for the female and 2 032.7 cm for the male map, corresponding to genome coverage of 77.6% and 72.2%, respectively.
基金supported by the National Natural Science Foundation of China(Grant Nos. 51179171 and 51079127)
文摘Periodic composites with band gaps that prevent the propagation of elastic waves in certain frequency ranges can be used to control waves for a variety of engineering applications. Although studies on the characteristics of these materials, which are called phononic crystals (PCs), have yielded a large number of positive results in recent years, there is still a lack of effective design methods. In this work, a multi-objective optimization approach based on the band gap mechanism and an intelligent algorithm is used to design a one-dimensional (1D) slab construction of PCs. The design aims to fit pre-determined bands by arranging the available materials properly. Obtained by analyzing the wave transmission in periodic layers, the objective functions are linked to the optimization program to obtain a proper solution set. The results of the numerical simulations demonstrate that without constructing complicated structures, the design method is able to produce PCs that overcome the limitations of two-component PCs and hence can feasibly and effectively achieve the design targets. The design approach presented in this paper can be extended to two-or three-dimensional systems and has great potential for the development of sound/ultrasound isolation structures, multiple band frequency filters, and other applications.
