The aim of this study is to assess the water microbiologic pollution in Durres＇s Harbour basin and to compare it with European standards. The comparison of heterotrophs and total coliforms level in sampling are the e...The aim of this study is to assess the water microbiologic pollution in Durres＇s Harbour basin and to compare it with European standards. The comparison of heterotrophs and total coliforms level in sampling are the essence of this research. The object of this study is done in four sampling areas of Durres＇s Harbour basin. In order to compare the level of water microbiologic pollution in four areas of Durres＇s Harbour basin, control area is also studied which is a beach area near the Harbour named Apollonia Beach. The sampling areas were： Ferry Terminal （FT）, Fishery Harbor （FH）, East Zone （EZ）, Fuel Quay （FQ） and Apollonia Beach （AB）, respectively. The period of sample-taking was July-October 2008. The strategy used for this purpose consisted in water insemination with coverage method by means of Petri＇s plates according to respective dilutions in culture media Plate Count Agar （PCA） for heterotrophs and MacConkey for total coliforms. The number of colonies that are formed determines the number of cells at the moment of water insemination, the number of heterotrophs in culture media PCA, respectively. The number of pink and red colonies that were formed determines the number of cells at the moment of water insemination, the number of total coliforms in culture media MacConkey, respectively. The measure ofheterotrophic bacteria and total coliforms used is Colony-Forming Units （CFU）/100 mL seawater. （AB） is within European standards. The richest area with heterotrophs is （FH）, which confirms the fact that it is the most polluted microbiologic area in the Harbour basin of Durres. The richest area with total coliforms is （FT）. The poorest area with heterotrophs and total coliforms is （FQ）.展开更多
Three hundred, one-day-old Japanese quail （Coturnixjaponica） divided into four groups, with three subgroups each, were fed a basal diet that served as control, or a basal diet containing 6 g calcium formate （CF）/k...Three hundred, one-day-old Japanese quail （Coturnixjaponica） divided into four groups, with three subgroups each, were fed a basal diet that served as control, or a basal diet containing 6 g calcium formate （CF）/kg or 2 g mannan oligosaccharides （MOS）/kg or both 6 g CF/kg plus 2 g MOS/kg. On 21st and 42nd days the pH of the quail crop, proventriculus, gizzard, duodenum, jejunum, ileum, large intestine and ceca were measured in situ and the total aerobic bacteria, lactic acid bacteria, Enterobacteriaceae and coliforms counts of the cecal content were recorded. Results showed that MOS decreased the cecal pH on the 42nd day and the total aerobic bacteria counts on 21st day. Moreover, CF decreased the cecal pH on the 42nd day and the lactic acid bacteria counts on 21st day. Interaction between MOS and CF was noticed on the cecal pH on the 42nd day, as the combined dietary addition of MOS and CF resulted in lower pH, compared to each separate addition. Also, interaction was noticed on the cecal bacterial counts on the 21st day, as the combined dietary addition of MOS and CF resulted in lower total aerobic bacteria, Enterobacteriacea and coliform counts, compared to each separate addition.展开更多
文摘The aim of this study is to assess the water microbiologic pollution in Durres＇s Harbour basin and to compare it with European standards. The comparison of heterotrophs and total coliforms level in sampling are the essence of this research. The object of this study is done in four sampling areas of Durres＇s Harbour basin. In order to compare the level of water microbiologic pollution in four areas of Durres＇s Harbour basin, control area is also studied which is a beach area near the Harbour named Apollonia Beach. The sampling areas were： Ferry Terminal （FT）, Fishery Harbor （FH）, East Zone （EZ）, Fuel Quay （FQ） and Apollonia Beach （AB）, respectively. The period of sample-taking was July-October 2008. The strategy used for this purpose consisted in water insemination with coverage method by means of Petri＇s plates according to respective dilutions in culture media Plate Count Agar （PCA） for heterotrophs and MacConkey for total coliforms. The number of colonies that are formed determines the number of cells at the moment of water insemination, the number of heterotrophs in culture media PCA, respectively. The number of pink and red colonies that were formed determines the number of cells at the moment of water insemination, the number of total coliforms in culture media MacConkey, respectively. The measure ofheterotrophic bacteria and total coliforms used is Colony-Forming Units （CFU）/100 mL seawater. （AB） is within European standards. The richest area with heterotrophs is （FH）, which confirms the fact that it is the most polluted microbiologic area in the Harbour basin of Durres. The richest area with total coliforms is （FT）. The poorest area with heterotrophs and total coliforms is （FQ）.
文摘Three hundred, one-day-old Japanese quail （Coturnixjaponica） divided into four groups, with three subgroups each, were fed a basal diet that served as control, or a basal diet containing 6 g calcium formate （CF）/kg or 2 g mannan oligosaccharides （MOS）/kg or both 6 g CF/kg plus 2 g MOS/kg. On 21st and 42nd days the pH of the quail crop, proventriculus, gizzard, duodenum, jejunum, ileum, large intestine and ceca were measured in situ and the total aerobic bacteria, lactic acid bacteria, Enterobacteriaceae and coliforms counts of the cecal content were recorded. Results showed that MOS decreased the cecal pH on the 42nd day and the total aerobic bacteria counts on 21st day. Moreover, CF decreased the cecal pH on the 42nd day and the lactic acid bacteria counts on 21st day. Interaction between MOS and CF was noticed on the cecal pH on the 42nd day, as the combined dietary addition of MOS and CF resulted in lower pH, compared to each separate addition. Also, interaction was noticed on the cecal bacterial counts on the 21st day, as the combined dietary addition of MOS and CF resulted in lower total aerobic bacteria, Enterobacteriacea and coliform counts, compared to each separate addition.
