针对轨道扣件车载实时定量检测中检测的速度与精度难以平衡以及设备空间有限的问题,提出一种基于边缘AI计算的改进YOLOv8(You Only Look Once version 8)轨道扣件定量检测方法,并进行了部署和测试。首先,为实现轻量化部署,优化YOLOv8网...针对轨道扣件车载实时定量检测中检测的速度与精度难以平衡以及设备空间有限的问题,提出一种基于边缘AI计算的改进YOLOv8(You Only Look Once version 8)轨道扣件定量检测方法,并进行了部署和测试。首先,为实现轻量化部署,优化YOLOv8网络结构,嵌入移动神经网络V3(MobileNetV3)轻量化网络,引入压缩激励(SE)注意力机制,并重构颈部网络,加入可变形卷积;其次,结合推理引擎加速方法优化重构的网络模型,并将其部署在Jetson AGX Xavier边缘AI计算设备上;最后,对分割结果进行像素级提取,引入最小外接矩阵,定量分析扣件的断裂程度和偏转角度,并优化检测结果。结果表明:改进后的轻量化网络参数数量减少了22%;在边缘AI计算设备上的帧率相对原YOLOv8模型提升了80%,达到58帧·s^(-1);通过定量分析结果对模型进行修正,平均精度达到了97.0%,满足检测车辆所需的最低45帧·s^(-1)检测要求,实现了轨道扣件的定量化实时检测。展开更多
[Objective] This study aimed to establish a quantitative real-time PCR （qRT-PCR） system for detecting the expression of rice beta-glucosidase gene Os1bglu4.[Method] The PCR was conducted with SYBR Green Ⅰ method,us...[Objective] This study aimed to establish a quantitative real-time PCR （qRT-PCR） system for detecting the expression of rice beta-glucosidase gene Os1bglu4.[Method] The PCR was conducted with SYBR Green Ⅰ method,using the primers of reference gene actin or ubiquitin.[Result] Actin was more suitable to be the reference gene than ubiquitin.More accurate results were obtained when the 100 ng cDNA template was added at a large volume and a lower concentration.The primer concentration in the range from 0.2 to 0.8 μmol/L we set had no significant influence on the results,so,0.4 μmol/L was selected as the optimal primer concentration in this study.The amplification efficiency was greatly reduced when the annealing temperature was set at 64 ℃,therefore,annealing temperature was set at 60 ℃.Compared with the reaction system of 25 μl,the fluorescence intensity was significantly lower but the CT value did not change greatly in 10 μl system.So,the 10 μl reaction system was selected,which significantly reduces the research costs for the detection of a large amount of samples in future study.展开更多
文摘针对轨道扣件车载实时定量检测中检测的速度与精度难以平衡以及设备空间有限的问题,提出一种基于边缘AI计算的改进YOLOv8(You Only Look Once version 8)轨道扣件定量检测方法,并进行了部署和测试。首先,为实现轻量化部署,优化YOLOv8网络结构,嵌入移动神经网络V3(MobileNetV3)轻量化网络,引入压缩激励(SE)注意力机制,并重构颈部网络,加入可变形卷积;其次,结合推理引擎加速方法优化重构的网络模型,并将其部署在Jetson AGX Xavier边缘AI计算设备上;最后,对分割结果进行像素级提取,引入最小外接矩阵,定量分析扣件的断裂程度和偏转角度,并优化检测结果。结果表明:改进后的轻量化网络参数数量减少了22%;在边缘AI计算设备上的帧率相对原YOLOv8模型提升了80%,达到58帧·s^(-1);通过定量分析结果对模型进行修正,平均精度达到了97.0%,满足检测车辆所需的最低45帧·s^(-1)检测要求,实现了轨道扣件的定量化实时检测。
基金Supported by Guizhou International Cooperation Project on Science and Technology[(2013)7040]the 20th Project of the Joint Committee on Scientific and Technical Cooperation between the Government of the Kingdom of Thailand and the Government of the People’s Republic of China (20-606J)the Fund from Suranaree University of Technology,Thailand (SUT3-304-54-12-29)
文摘[Objective] This study aimed to establish a quantitative real-time PCR （qRT-PCR） system for detecting the expression of rice beta-glucosidase gene Os1bglu4.[Method] The PCR was conducted with SYBR Green Ⅰ method,using the primers of reference gene actin or ubiquitin.[Result] Actin was more suitable to be the reference gene than ubiquitin.More accurate results were obtained when the 100 ng cDNA template was added at a large volume and a lower concentration.The primer concentration in the range from 0.2 to 0.8 μmol/L we set had no significant influence on the results,so,0.4 μmol/L was selected as the optimal primer concentration in this study.The amplification efficiency was greatly reduced when the annealing temperature was set at 64 ℃,therefore,annealing temperature was set at 60 ℃.Compared with the reaction system of 25 μl,the fluorescence intensity was significantly lower but the CT value did not change greatly in 10 μl system.So,the 10 μl reaction system was selected,which significantly reduces the research costs for the detection of a large amount of samples in future study.
