[目的]对地衣芽孢杆菌的豆粕固态发酵实验室小试工艺中豆粕的粒度和容器内堆料条件进行优化。[方法]在建立豆粕内活菌的有效计数方法的基础上,分析不同混匀方式对发酵豆粕中菌量计数的影响,并研究不同发酵容器、豆粕粒径和堆料厚度对发...[目的]对地衣芽孢杆菌的豆粕固态发酵实验室小试工艺中豆粕的粒度和容器内堆料条件进行优化。[方法]在建立豆粕内活菌的有效计数方法的基础上,分析不同混匀方式对发酵豆粕中菌量计数的影响,并研究不同发酵容器、豆粕粒径和堆料厚度对发酵豆粕菌浓度的影响。[结果]发酵后豆粕在PBS振荡1 min能最大程度地释放其中的细菌,以便后续计数;以4层报纸封口的50 m L离心管为发酵容器,采用经80目筛孔的豆粕颗粒和1.5 cm堆料厚度能获得最优的实验室小试发酵效果,菌浓度能达到1.65×1010~1.76×1010CFU/g。[结论]研究为发酵豆粕实验室小试方法提供了技术支持。展开更多
The present researches mentioned questions of economic use of domestic triticale and their influences on a phytosanitory condition and fertility of an irrigated arable land in the Chuy Valley of Kyrgyzstan. The result...The present researches mentioned questions of economic use of domestic triticale and their influences on a phytosanitory condition and fertility of an irrigated arable land in the Chuy Valley of Kyrgyzstan. The result of the work consists of four tables, purpose of the work was to discover roots systems, nutrion value and the main laboratory testing of samples were conducted at the Humboldt University in Berlin, which was completed under the high and compliant standards of the world. The present research conducted on triticale varieties was firstly studied in Kyrgyzstan.展开更多
A small strain unified hardening(SSUH) model is proposed in the present study to tackle the small strain behavior of clay. The model is an extension of the unified hardening(UH) model for overconsolidated(OC) clays ac...A small strain unified hardening(SSUH) model is proposed in the present study to tackle the small strain behavior of clay. The model is an extension of the unified hardening(UH) model for overconsolidated(OC) clays accounting for the small strain stiffness. The new features of the SSUH model over the UH model include:(a) a new elastic hysteretic stress-strain relationship to evaluate the stiffness degradation at small strains and to generate the hysteresis loop under cyclic loading condition;(b) a revised unified hardening parameter to enhance the plastic stiffness at small strains; and(c) a new overconsolidation parameter, which is crucial to make the UH model working with the elastic hysteretic stress-strain relationship effectively. With these enhancements, the SSUH model can describe a high initial stiffness and the highly nonlinear stress-strain relationship at small strains, in addition to the shear dilatancy and strain hardening/softening behaviors of OC clays at large strains. In comparison with the Modified Cam-clay(MCC) model, the proposed model needs two more small strain related parameters, which can be easily obtained from laboratory tests. Finally, some drained triaxial compression tests at large strains, drained triaxial compression/extension tests at small strains, an undrained compression test at small strains and a drained cyclic constant radial stress test are employed to validate the new model.展开更多
文摘[目的]对地衣芽孢杆菌的豆粕固态发酵实验室小试工艺中豆粕的粒度和容器内堆料条件进行优化。[方法]在建立豆粕内活菌的有效计数方法的基础上,分析不同混匀方式对发酵豆粕中菌量计数的影响,并研究不同发酵容器、豆粕粒径和堆料厚度对发酵豆粕菌浓度的影响。[结果]发酵后豆粕在PBS振荡1 min能最大程度地释放其中的细菌,以便后续计数;以4层报纸封口的50 m L离心管为发酵容器,采用经80目筛孔的豆粕颗粒和1.5 cm堆料厚度能获得最优的实验室小试发酵效果,菌浓度能达到1.65×1010~1.76×1010CFU/g。[结论]研究为发酵豆粕实验室小试方法提供了技术支持。
文摘The present researches mentioned questions of economic use of domestic triticale and their influences on a phytosanitory condition and fertility of an irrigated arable land in the Chuy Valley of Kyrgyzstan. The result of the work consists of four tables, purpose of the work was to discover roots systems, nutrion value and the main laboratory testing of samples were conducted at the Humboldt University in Berlin, which was completed under the high and compliant standards of the world. The present research conducted on triticale varieties was firstly studied in Kyrgyzstan.
基金supported by the National Program on Key Basic Research Project of China(973 ProgramGrant No.2014CB047001)+2 种基金the National Natural Science Foundation of China(Grant Nos.1127203151179003 and 41372285)Beijing Natural Science Foundation(Grant No.8132042)
文摘A small strain unified hardening(SSUH) model is proposed in the present study to tackle the small strain behavior of clay. The model is an extension of the unified hardening(UH) model for overconsolidated(OC) clays accounting for the small strain stiffness. The new features of the SSUH model over the UH model include:(a) a new elastic hysteretic stress-strain relationship to evaluate the stiffness degradation at small strains and to generate the hysteresis loop under cyclic loading condition;(b) a revised unified hardening parameter to enhance the plastic stiffness at small strains; and(c) a new overconsolidation parameter, which is crucial to make the UH model working with the elastic hysteretic stress-strain relationship effectively. With these enhancements, the SSUH model can describe a high initial stiffness and the highly nonlinear stress-strain relationship at small strains, in addition to the shear dilatancy and strain hardening/softening behaviors of OC clays at large strains. In comparison with the Modified Cam-clay(MCC) model, the proposed model needs two more small strain related parameters, which can be easily obtained from laboratory tests. Finally, some drained triaxial compression tests at large strains, drained triaxial compression/extension tests at small strains, an undrained compression test at small strains and a drained cyclic constant radial stress test are employed to validate the new model.
