th instar silkworms were infected with recombinant baculovirus containing phytase gene or wild type BmNPV at 48hr after ecdysis,then treated with 100ppm Juvenile hormone.It showed that the expression level of phytase ...th instar silkworms were infected with recombinant baculovirus containing phytase gene or wild type BmNPV at 48hr after ecdysis,then treated with 100ppm Juvenile hormone.It showed that the expression level of phytase gene and polyhedrin gene per silkworm was increased by 30% and 40%,respectively.The LT 50 was lengthened for more than 4h,and the average weight of sick silkworm was increased by 10%.The results indicated that the improvement of expression efficiency of phytase gene and polyhedrin gene was mainly caused by longer time of virus replication in silkworm after the treatment of Juvenile hormone.展开更多
口蹄疫（Foot and mouth disease, FMD）是由FMD病毒（FMDV）引起的偶蹄动物的一种急性、热性、高度接触性人兽共患传染病。世界动物卫生组织（OIE）将其列为必须通报的动物传染病，我国将其列为一类动物传染病。该病传播途径多，传播...口蹄疫（Foot and mouth disease, FMD）是由FMD病毒（FMDV）引起的偶蹄动物的一种急性、热性、高度接触性人兽共患传染病。世界动物卫生组织（OIE）将其列为必须通报的动物传染病，我国将其列为一类动物传染病。该病传播途径多，传播速度快。目前除了北美、澳洲等地区外，世界范围内多次暴发流行。最近几年，一些已经消灭FMD的国家又再次爆发，FMD防制形势依然严峻。在FMD防制中，加强流行病学监测和采取疫苗免疫是防制其发生的主要措施和关键环节。因此，特异性诊断抗原的制备和新型疫苗的开发就显得尤为重要。展开更多
文摘th instar silkworms were infected with recombinant baculovirus containing phytase gene or wild type BmNPV at 48hr after ecdysis,then treated with 100ppm Juvenile hormone.It showed that the expression level of phytase gene and polyhedrin gene per silkworm was increased by 30% and 40%,respectively.The LT 50 was lengthened for more than 4h,and the average weight of sick silkworm was increased by 10%.The results indicated that the improvement of expression efficiency of phytase gene and polyhedrin gene was mainly caused by longer time of virus replication in silkworm after the treatment of Juvenile hormone.
文摘口蹄疫（Foot and mouth disease, FMD）是由FMD病毒（FMDV）引起的偶蹄动物的一种急性、热性、高度接触性人兽共患传染病。世界动物卫生组织（OIE）将其列为必须通报的动物传染病，我国将其列为一类动物传染病。该病传播途径多，传播速度快。目前除了北美、澳洲等地区外，世界范围内多次暴发流行。最近几年，一些已经消灭FMD的国家又再次爆发，FMD防制形势依然严峻。在FMD防制中，加强流行病学监测和采取疫苗免疫是防制其发生的主要措施和关键环节。因此，特异性诊断抗原的制备和新型疫苗的开发就显得尤为重要。
