Full length complementary DNAs (cDNA) are crucial for the functional annotation of genes. Several novel experimental approaches for selective enrichment of full length cDNAs are outlined in parallels. Some kinds of re...Full length complementary DNAs (cDNA) are crucial for the functional annotation of genes. Several novel experimental approaches for selective enrichment of full length cDNAs are outlined in parallels. Some kinds of reverse transcriptases and high fidelity PCR enzymes are introduced for promising high efficiency of accurate full length cDNA synthesis. The temperature profiles of the reverse transcription and PCR are also discussed.展开更多
病毒的末端结合蛋白VPg(Viral Protein Genome-linked)是一种多功能蛋白,存在于多种病毒中,与病毒基因组RNA5′-末端共价连接,在病毒的生命周期中以不同的剪切形式存在并行使不同的功能,参与病毒的复制、翻译等基本的生命活动。主要对...病毒的末端结合蛋白VPg(Viral Protein Genome-linked)是一种多功能蛋白,存在于多种病毒中,与病毒基因组RNA5′-末端共价连接,在病毒的生命周期中以不同的剪切形式存在并行使不同的功能,参与病毒的复制、翻译等基本的生命活动。主要对目前关于VPg如何参与病毒的复制和翻译过程,以及在病毒蛋白质成熟和病毒移动过程中如何发挥作用的研究结果进行了综述。展开更多
文摘Full length complementary DNAs (cDNA) are crucial for the functional annotation of genes. Several novel experimental approaches for selective enrichment of full length cDNAs are outlined in parallels. Some kinds of reverse transcriptases and high fidelity PCR enzymes are introduced for promising high efficiency of accurate full length cDNA synthesis. The temperature profiles of the reverse transcription and PCR are also discussed.
文摘病毒的末端结合蛋白VPg(Viral Protein Genome-linked)是一种多功能蛋白,存在于多种病毒中,与病毒基因组RNA5′-末端共价连接,在病毒的生命周期中以不同的剪切形式存在并行使不同的功能,参与病毒的复制、翻译等基本的生命活动。主要对目前关于VPg如何参与病毒的复制和翻译过程,以及在病毒蛋白质成熟和病毒移动过程中如何发挥作用的研究结果进行了综述。
