《土壤检测 第14部分:土壤有效硫的测定》(NY/T 1121.14-2023)的比浊法是土壤有效硫的测定分析方法之一,相对其他分光光度法,它的相关系数r要求也不高,只要求r ≥ 0.995,未要求r ≥ 0.999,所以本方法还有很多优化空间。笔者经过长时间...《土壤检测 第14部分:土壤有效硫的测定》(NY/T 1121.14-2023)的比浊法是土壤有效硫的测定分析方法之一,相对其他分光光度法,它的相关系数r要求也不高,只要求r ≥ 0.995,未要求r ≥ 0.999,所以本方法还有很多优化空间。笔者经过长时间的摸索和总结,整理出本优化方法供分析人员参考,以帮助大家在今后的分析操作中能更便捷、快速又准确地进行。优化后的方法减少了中间转移操作,用超声代替了磁力搅拌,提高了工作效率,关键是可以提升准确性,重现性更好。The turbidity method in “Soil Testing Part 14: Determination of Soil Available Sulfur” (NY/T 1121.14-2023) is one of the methods for determining and analyzing soil available sulfur. Compared with other spectrophotometric methods, its correlation coefficient r requirement is not high. As long as r ≥ 0.995 is required, r ≥ 0.999 is not required. Therefore, there is still a lot of room for optimization in this method. After a long period of exploration and summarization, the authors have compiled this optimization method for analysts to refer to, in order to help everyone perform analysis operations more conveniently, quickly, and accurately in the future. The optimized method reduces intermediate transfer operations and replaces magnetic stirring with ultrasound, improving work efficiency. The key is that it can improve accuracy and reproducibility.展开更多
[目的]分析DNA、引物,dNTPs、Taq DNA聚合酶4种因素对雷公笋SCoT-PCR扩增结果的影响,通过最优的SCoT-PCR反应体系筛选多态性引物,为雷公笋种质资源分子标记研究提供条件。[方法]以海南雷公笋为材料,采用单因素试验和正交试验方法。[结果...[目的]分析DNA、引物,dNTPs、Taq DNA聚合酶4种因素对雷公笋SCoT-PCR扩增结果的影响,通过最优的SCoT-PCR反应体系筛选多态性引物,为雷公笋种质资源分子标记研究提供条件。[方法]以海南雷公笋为材料,采用单因素试验和正交试验方法。[结果]Taq酶对雷公笋SCoT-PCR扩增的影响最大,其次是模板DNA和dNTPs,最后是引物;最优反应体系为20μL体系中40 ng DNA,0.30μmol/L引物,0.25 mmol/L dNTPs,3.00 U Taq酶。经验证,该体系获得的扩增产物清晰稳定;应用该体系从40条SCoT引物筛选出8条多态性好,且适合雷公笋扩增的引物,多态性条带占62.64%。[结论]该研究为使用SCoT分子标记技术对雷公笋开展深入研究提供了重要的理论基础和技术支持。展开更多
文摘《土壤检测 第14部分:土壤有效硫的测定》(NY/T 1121.14-2023)的比浊法是土壤有效硫的测定分析方法之一,相对其他分光光度法,它的相关系数r要求也不高,只要求r ≥ 0.995,未要求r ≥ 0.999,所以本方法还有很多优化空间。笔者经过长时间的摸索和总结,整理出本优化方法供分析人员参考,以帮助大家在今后的分析操作中能更便捷、快速又准确地进行。优化后的方法减少了中间转移操作,用超声代替了磁力搅拌,提高了工作效率,关键是可以提升准确性,重现性更好。The turbidity method in “Soil Testing Part 14: Determination of Soil Available Sulfur” (NY/T 1121.14-2023) is one of the methods for determining and analyzing soil available sulfur. Compared with other spectrophotometric methods, its correlation coefficient r requirement is not high. As long as r ≥ 0.995 is required, r ≥ 0.999 is not required. Therefore, there is still a lot of room for optimization in this method. After a long period of exploration and summarization, the authors have compiled this optimization method for analysts to refer to, in order to help everyone perform analysis operations more conveniently, quickly, and accurately in the future. The optimized method reduces intermediate transfer operations and replaces magnetic stirring with ultrasound, improving work efficiency. The key is that it can improve accuracy and reproducibility.
文摘[目的]分析DNA、引物,dNTPs、Taq DNA聚合酶4种因素对雷公笋SCoT-PCR扩增结果的影响,通过最优的SCoT-PCR反应体系筛选多态性引物,为雷公笋种质资源分子标记研究提供条件。[方法]以海南雷公笋为材料,采用单因素试验和正交试验方法。[结果]Taq酶对雷公笋SCoT-PCR扩增的影响最大,其次是模板DNA和dNTPs,最后是引物;最优反应体系为20μL体系中40 ng DNA,0.30μmol/L引物,0.25 mmol/L dNTPs,3.00 U Taq酶。经验证,该体系获得的扩增产物清晰稳定;应用该体系从40条SCoT引物筛选出8条多态性好,且适合雷公笋扩增的引物,多态性条带占62.64%。[结论]该研究为使用SCoT分子标记技术对雷公笋开展深入研究提供了重要的理论基础和技术支持。
