目的：探讨胃癌及癌前病变中微卫星不稳定（MSI）的变化．方法：饱和氯化钠法提取组织DNA，PCR-SSCP变性聚丙烯酰胺凝胶电泳检测MSI，根据规定标准来判定microsatellite stable（MSS），low frequency MSI（MSI-L）和high-frequency MSI...目的：探讨胃癌及癌前病变中微卫星不稳定（MSI）的变化．方法：饱和氯化钠法提取组织DNA，PCR-SSCP变性聚丙烯酰胺凝胶电泳检测MSI，根据规定标准来判定microsatellite stable（MSS），low frequency MSI（MSI-L）和high-frequency MSI（MSI-H），计算总MSI率．结果：MSI在萎缩性胃炎，肠上皮化生，不典型增生，早期胃癌和进展期胃癌中检出率分别为13．3％、16．7％、23．3％、30％和40％，三种癌前期病变和早期胃癌之间有显著差异（χ^2值分别为4．364、2．522、3．089，P〈0．05），早期胃癌与进展期胃癌没有显著差异（χ^2值为0．071 P〉0．05），高分化腺癌中MSI的阳性率明显高于低分化腺癌（χ^2值为4．022，P〈0．05）．结论：MSI是胃癌发生中的早期分子标志，其阳性的胃癌恶性程度相对较低．展开更多
Objective: To investigate the early effects of hypertonic and isotonic saline solutions on apoptosis of intestinal mucosa in rats with hemorrhagic shock. Methods: A model of rat with severe hemorrhagic shock was estab...Objective: To investigate the early effects of hypertonic and isotonic saline solutions on apoptosis of intestinal mucosa in rats with hemorrhagic shock. Methods: A model of rat with severe hemorrhagic shock was established in 21 Sprague-Dawley (SD) rats. The rats were randomly divided into the sham group, normal saline resuscitation (NS) group, and hypertonic saline resuscitation (HTS) group, with 7 in each group. We detected and compared the apoptosis in small intestinal mucosa of rats after hemorrhagic shock and resuscitation by terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL), FITC (fluo- rescein-iso-thiocyanate)-Annexin V/PI (propidium iodide) double staining method, and flow cytometry. Results: In the early stage of hemorrhagic shock and resuscitation, marked apoptosis of small intestinal mucosa in the rats of both NS and HTS groups was observed. The numbers of apoptotic cells in these two groups were significantly greater than that in the sham group (P<0.01). In the HTS group, the apoptic cells significantly decreased, compared with the NS group (P<0.01). Conclusion: In this rat model of severe hemorrhagic shock, the HTS resuscitation of small volume is more effective than the NS resuscitation in reducing apoptosis of intestinal mucosa in rats, which may improve the prognosis of trauma.展开更多
Sodium hypochlorite was used to treat the standard molybdenum concentrates; the oxidization conditions for sodium hypochlorite were investigated, and the electro-oxidation process was performed. The results indicate t...Sodium hypochlorite was used to treat the standard molybdenum concentrates; the oxidization conditions for sodium hypochlorite were investigated, and the electro-oxidation process was performed. The results indicate that in the suitable conditions, such as temperature around 25℃, NaCl concentration 4.0mol/L, mass ratio of ore slurry liquid to solid （mL/mS） 20, electric charge per gram Mo 0.522C, pH value of original slurry 8, anodic current density 700A·m-2 （cell potential 2.72.9V）, the Mo leaching rate and the current efficiency reach 98% and 36%, respectively. In order to overcome some shortages of the electro-oxidation process, such as low current efficiency, low Mo concentration in the leaching solution, ultrasonic was adopted to intensify the leaching process. The results show that the Mo leaching rate exceeds 98%, current efficiency increases from 36% to 50% and the Mo concentration in the leaching solution reaches about 60g/L at low mL/mS of 8 and low electric charge of 0.373C.展开更多
基金Project (No. 20061420) supported by the Education and Research Foundation of Zhejiang Province, China
文摘Objective: To investigate the early effects of hypertonic and isotonic saline solutions on apoptosis of intestinal mucosa in rats with hemorrhagic shock. Methods: A model of rat with severe hemorrhagic shock was established in 21 Sprague-Dawley (SD) rats. The rats were randomly divided into the sham group, normal saline resuscitation (NS) group, and hypertonic saline resuscitation (HTS) group, with 7 in each group. We detected and compared the apoptosis in small intestinal mucosa of rats after hemorrhagic shock and resuscitation by terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL), FITC (fluo- rescein-iso-thiocyanate)-Annexin V/PI (propidium iodide) double staining method, and flow cytometry. Results: In the early stage of hemorrhagic shock and resuscitation, marked apoptosis of small intestinal mucosa in the rats of both NS and HTS groups was observed. The numbers of apoptotic cells in these two groups were significantly greater than that in the sham group (P<0.01). In the HTS group, the apoptic cells significantly decreased, compared with the NS group (P<0.01). Conclusion: In this rat model of severe hemorrhagic shock, the HTS resuscitation of small volume is more effective than the NS resuscitation in reducing apoptosis of intestinal mucosa in rats, which may improve the prognosis of trauma.
文摘Sodium hypochlorite was used to treat the standard molybdenum concentrates; the oxidization conditions for sodium hypochlorite were investigated, and the electro-oxidation process was performed. The results indicate that in the suitable conditions, such as temperature around 25℃, NaCl concentration 4.0mol/L, mass ratio of ore slurry liquid to solid （mL/mS） 20, electric charge per gram Mo 0.522C, pH value of original slurry 8, anodic current density 700A·m-2 （cell potential 2.72.9V）, the Mo leaching rate and the current efficiency reach 98% and 36%, respectively. In order to overcome some shortages of the electro-oxidation process, such as low current efficiency, low Mo concentration in the leaching solution, ultrasonic was adopted to intensify the leaching process. The results show that the Mo leaching rate exceeds 98%, current efficiency increases from 36% to 50% and the Mo concentration in the leaching solution reaches about 60g/L at low mL/mS of 8 and low electric charge of 0.373C.
