期刊文献+
共找到3篇文章
< 1 >
每页显示 20 50 100
乔松根特异启动子PmPgPR10驱动Na^+/H^+逆转运蛋白提高转基因水稻的耐盐性 被引量:1
1
作者 武丽敏 陈维 +4 位作者 赵艳 冯尚国 应奇才 刘俊君 王慧中 《中国水稻科学》 CAS CSCD 北大核心 2012年第6期643-650,共8页
为获得抗盐水稻,将小麦液泡膜Na+/H+逆向转运蛋白基因TaNHX2与乔松(Pinus griffithii)根诱导型特异表达启动子PmPgPR10融合(PmPgPR10∷TaNHX2)并转化水稻,以研究PmPgPR10启动子对TaNHX2基因表达的影响以及转基因植物的耐盐性。PCR、Sout... 为获得抗盐水稻,将小麦液泡膜Na+/H+逆向转运蛋白基因TaNHX2与乔松(Pinus griffithii)根诱导型特异表达启动子PmPgPR10融合(PmPgPR10∷TaNHX2)并转化水稻,以研究PmPgPR10启动子对TaNHX2基因表达的影响以及转基因植物的耐盐性。PCR、Southern和实时PCR试验结果表明,PmPgPR10∷TaNHX2基因已通过农杆菌介导法整合进水稻基因组,而且外源基因已在受体细胞中正确表达。在盐胁迫处理时,转PmPgPR10基因植株的耐盐性以及外源基因的表达量显著高于对照植株,说明PmPgPR10启动子可以调控TaNHX2基因在根中特异表达。为了进一步分析转基因植株耐盐机理,比较了日本晴和转基因T3代植株中液泡腺苷三磷酸酶(V-ATPase)和液泡焦磷酸酶(V-PPase)活性,发现转PmPgPR10∷TaNHX2基因水稻的V-ATPase和V-PPase酶活性显著高于非转基因对照,说明V-ATPase和V-PPase活性提高在转TaNHX2基因水稻耐盐性过程中发挥重要作用。在盐胁迫处理时,V-ATPase和V-PPase活性只能在转基因植株的根中但不能在叶片中被检测到,进一步说明PmPgPR10启动子在根中特异性表达。因此,PmPgPR10具有在根中增强下游TaNHX2基因表达,并显著提高转基因植株耐盐性的能力。 展开更多
关键词 启动子 乔松 水稻耐盐性 Na+ H+逆向转运蛋白
在线阅读 下载PDF
基于UbD理论的溶液项目化学习设计与实践——以“测定水稻的耐盐性”为例
2
作者 仇永红 刘晓娟 +2 位作者 宗厉 李春晖 王环峰 《教育与装备研究》 2023年第9期11-18,共8页
教学中有一个常见误区是重活动轻结果的“活动导向”教学设计,这样的教学可能导致学生“只动手不动脑”,UbD理论的宗旨是以学习目标为起点逆向设计教学,促进学生达成有意义学习、深度理解、迁移运用,契合新课标倡导的课程理念。文章基于... 教学中有一个常见误区是重活动轻结果的“活动导向”教学设计,这样的教学可能导致学生“只动手不动脑”,UbD理论的宗旨是以学习目标为起点逆向设计教学,促进学生达成有意义学习、深度理解、迁移运用,契合新课标倡导的课程理念。文章基于UbD理论,就九年级化学“溶液”的内容制定“测定水稻的耐盐性”项目化学习,取得了良好的教学效果。 展开更多
关键词 逆向设计理论 项目化学习 溶液 测定水稻
在线阅读 下载PDF
Salt Tolerance of 15 Rice (Oryza sativa L.) Varieties in the Office du Niger Zone of Mali
3
作者 Minamba Bagayoko Brehima Kamissoko Mamadou M. Coulibaly 《Journal of Agricultural Science and Technology(B)》 2014年第3期224-236,共13页
From 2003 to 2005, in pot and field experiments, rice response to salinity stress of 15 rice varieties was studied at germination and young seedling stages using salt affected soils collected from rice production area... From 2003 to 2005, in pot and field experiments, rice response to salinity stress of 15 rice varieties was studied at germination and young seedling stages using salt affected soils collected from rice production areas in the Office du Niger zone of Mali. The rice varieties were composed of 10 rice genotypes from the breeding program of Mali and five from West African Rice Development Association (WARDA) program (Saint Louis, Senegal). Soil samples were collected from the visually affected soils which were characterized by the appearance of white or black efflorescence on the soil surface. In pot experiments, the genotypes were allowed to germinate in both affected soil types (white efflorescence and black efflorescence) and salt effects on plant seedling growth were observed. Results showed that all varieties were significantly sensitive to salinity stress based on germination, young seedling shoot and root dry weights. Among the rice varieties, the most salt tolerant variety was BG90-2 (a high yielding genotype from the Institut d'Economie Rurale (IER) breeding program) while the most sensitive variety was Telimani (also from the breeding program of IER). All other varieties were intermediary between these two genotypes. A three year field experiment conducted in a highly affected area near Niono confirmed the results of the pot experiment. The relatively salt tolerant genotypes were found in both Malian (BG90-2, Kogoni91-1, SK51-5-2) and WARDA (Was30-11-1-1-4-6-1B) rice breeding programs. 展开更多
关键词 Salinity stress VARIETIES salt tolerance salinisation/alkalinisation irrigation regimes.
在线阅读 下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部