[Objective] This study aimed to improve density and practicality of the ge- netic map of sunflower （Helianthus annuus L.） by adding some SSR markers. [Method] A total of 123 F8 RILs populations derived from a cross ...[Objective] This study aimed to improve density and practicality of the ge- netic map of sunflower （Helianthus annuus L.） by adding some SSR markers. [Method] A total of 123 F8 RILs populations derived from a cross between PAC-2 and RHA-266 were selected as the materials, and a total of 300 pairs of SSR primers were used to screen polymorphic markers between the parents and some of their RILs, and finally 51 pairs of the primers producing polymorphic patterns were selected to construct the genetic map of the RILs populations. [Result] Nineteen pairs of SSR primers did not generate polymorphic patterns or any bands, and 32 SSR pairs showed polymorphism. Thirty-five alleles which distributed in the 15 link- age groups of the maps were detected. The new map covered a total length of 2 914.5 cM, 7.5 cM longer than that of the original map. The average marker interval is 8.1 cM replacing original 9.0 cM. [Conclusion] This study provided reference for genetic map integration and molecular marker assisted selection of sunflower.展开更多
基金Supported by Major Transgenic Project of Ministry of Agriculture of China(No. 2011ZX08011-006)Research Fund for the Youth of Sichuan Academy of Agricultural Sciences(2012QQJJ-027)~~
文摘[Objective] This study aimed to improve density and practicality of the ge- netic map of sunflower （Helianthus annuus L.） by adding some SSR markers. [Method] A total of 123 F8 RILs populations derived from a cross between PAC-2 and RHA-266 were selected as the materials, and a total of 300 pairs of SSR primers were used to screen polymorphic markers between the parents and some of their RILs, and finally 51 pairs of the primers producing polymorphic patterns were selected to construct the genetic map of the RILs populations. [Result] Nineteen pairs of SSR primers did not generate polymorphic patterns or any bands, and 32 SSR pairs showed polymorphism. Thirty-five alleles which distributed in the 15 link- age groups of the maps were detected. The new map covered a total length of 2 914.5 cM, 7.5 cM longer than that of the original map. The average marker interval is 8.1 cM replacing original 9.0 cM. [Conclusion] This study provided reference for genetic map integration and molecular marker assisted selection of sunflower.
