Two variants of diet composition were prepared to evaluate the susceptibility of ECBs to CrylAb toxin as follows： 1） 38-0600 Stonefly Heliothis Diet mixed with purified CrylAb protein and 2） 38-0600 Stonefly Heliot...Two variants of diet composition were prepared to evaluate the susceptibility of ECBs to CrylAb toxin as follows： 1） 38-0600 Stonefly Heliothis Diet mixed with purified CrylAb protein and 2） 38-0600 Stonefly Heliothis Diet mixed with lyophylized leaves of Bt maize MON 810-YieldGard. A method of sample preparation and extraction of Bt toxin for reproducible ELISA quantification were optimized. The qualitative DAS-ELISA kit from Agdia was optimized for use in quantitative analysis of Cry lAb toxin. The mortality of ECB larvae from the laboratory strain on the diet with CrylAb toxin did not differ significantly from the mortality on the diet with Bt maize leaves with the same rate of Cry lAb toxin. Similarly, the mortality of the ECB larvae from the field population on the diet with Bt maize leaves did not differ significantly from the mortality of ECB larvae from the laboratory strain on the same type of diet. Therefore, the incorporation of Bt maize leaves into the diet did not influence the efficacy of CrylAb toxin against ECBs. Using this method, a susceptibility of one field population of ECBs from the Czech Republic to CrylAb toxin was determined （LC50 of 2.16 μg of Cry 1Ab g^-1 of diet）.展开更多
Along with the rich material life, the change of the diet structure, people＇s shape also began to change.With performance m females is the body fat＇s increasing, the shape is not beautiful, therefore, body sculpting...Along with the rich material life, the change of the diet structure, people＇s shape also began to change.With performance m females is the body fat＇s increasing, the shape is not beautiful, therefore, body sculpting underwear has become the first choice for women to pursue beauty.To the requirement of sculpting body underwear is also becoming higher and higher for women.Comfortable and fashion is the sign of measuring a sculpting body underwear quality. This paper introduces the development history of sculpting body underwear, sculpting body underwear comfort evaluation index.And from the three aspects of the human body, clothing, environment do research to sculpting body underwear about the influence factors of wearing comfort.lt provides the certain reference value for consumers to purchase the sculpting body underwear and for enterprise to product sculpting body underwear.展开更多
文摘Two variants of diet composition were prepared to evaluate the susceptibility of ECBs to CrylAb toxin as follows： 1） 38-0600 Stonefly Heliothis Diet mixed with purified CrylAb protein and 2） 38-0600 Stonefly Heliothis Diet mixed with lyophylized leaves of Bt maize MON 810-YieldGard. A method of sample preparation and extraction of Bt toxin for reproducible ELISA quantification were optimized. The qualitative DAS-ELISA kit from Agdia was optimized for use in quantitative analysis of Cry lAb toxin. The mortality of ECB larvae from the laboratory strain on the diet with CrylAb toxin did not differ significantly from the mortality on the diet with Bt maize leaves with the same rate of Cry lAb toxin. Similarly, the mortality of the ECB larvae from the field population on the diet with Bt maize leaves did not differ significantly from the mortality of ECB larvae from the laboratory strain on the same type of diet. Therefore, the incorporation of Bt maize leaves into the diet did not influence the efficacy of CrylAb toxin against ECBs. Using this method, a susceptibility of one field population of ECBs from the Czech Republic to CrylAb toxin was determined （LC50 of 2.16 μg of Cry 1Ab g^-1 of diet）.
文摘Along with the rich material life, the change of the diet structure, people＇s shape also began to change.With performance m females is the body fat＇s increasing, the shape is not beautiful, therefore, body sculpting underwear has become the first choice for women to pursue beauty.To the requirement of sculpting body underwear is also becoming higher and higher for women.Comfortable and fashion is the sign of measuring a sculpting body underwear quality. This paper introduces the development history of sculpting body underwear, sculpting body underwear comfort evaluation index.And from the three aspects of the human body, clothing, environment do research to sculpting body underwear about the influence factors of wearing comfort.lt provides the certain reference value for consumers to purchase the sculpting body underwear and for enterprise to product sculpting body underwear.
