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建鲤生长激素促泌素受体1基因的特性及其与增重相关SNP位点的筛选 被引量:9
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作者 俞菊华 李红霞 +2 位作者 李建林 唐永凯 董在杰 《中国水产科学》 CAS CSCD 北大核心 2012年第3期390-398,共9页
GHS-R基因在哺乳类为候选的肥胖和生长数量性状位点。本实验使用RT-PCR及PCR的方法,从建鲤(Cyprinus carpio var.jian)分离到2个jlGHS-R1s,称为jlGHS-R1a和jlGHS-R1b(分别简称1a和1b)。jlGHS-R1s的阅读框编码360个氨基酸,两者间相似性高... GHS-R基因在哺乳类为候选的肥胖和生长数量性状位点。本实验使用RT-PCR及PCR的方法,从建鲤(Cyprinus carpio var.jian)分离到2个jlGHS-R1s,称为jlGHS-R1a和jlGHS-R1b(分别简称1a和1b)。jlGHS-R1s的阅读框编码360个氨基酸,两者间相似性高达96%;另外还存在2个jlGHS-R1s的转录变体(分别简称1a'和1b'),选择性切割了翻译起始碱基后第490-680 nt的191 bp片段,该片段两端碱基分别为gt-ag,这种选择性切割导致翻译提前终止,仅翻译184个氨基酸,包括前面3个半跨膜区,这与已报道的罗非鱼等的保留内含子的转录变体不同。jlGHS-R1s的阅读框被1个内含子分隔,该内含子位于第260个氨基酸密码子的第1和第2个碱基之间,1a和1b的内含子长度分别为676 bp和885 bp。使用分段PCR在建鲤群体的1a和1b基因上共找到32个SNP位点,构建PCR-RFLP法对其中9个SNPs进行基因型检测,结果发现各种基因型在322尾建鲤中均有出现,但存在着明显的偏分布。与增重的相关分析表明,位点1a-I_C386T和1b-E1_G159T与雌雄鱼鱼种和成鱼阶段增重分别呈极显著(P<0.01)和显著(P<0.05)相关,CC型和GG型个体增重最快,另外有5个位点则与其中的某阶段或者某性别的增重相关,为了确定上述所得标记的适用性,选取其中4个标记在另外7个家系共610尾鱼中检测,结果 C386T和G159T还是与增重呈极显著(P<0.01)和显著(P<0.05)相关,可用于建鲤分子育种。 展开更多
关键词 建鲤 生长激素促泌素受体 单核苷酸多态位点 生长相关分子标记
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Molecular Related Indexes Assessment and Traits Correlation Analysis of Turbot(Scophthalmus maximus L.) by Using SSR Marker Correlation Coefficient in Turbot
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作者 Yu MA Yulong HU +1 位作者 Liyong XU Weiji WANG 《Agricultural Science & Technology》 CAS 2014年第6期895-900,共6页
Five molecular related indexes: MOL, SIM, d2, H0and PIC of 15 turbot parent pairs were estimated by using 10 SSR loci, which were used in correlation analysis with growth traits, DIL and DIW, of family filial from tho... Five molecular related indexes: MOL, SIM, d2, H0and PIC of 15 turbot parent pairs were estimated by using 10 SSR loci, which were used in correlation analysis with growth traits, DIL and DIW, of family filial from those 15 parent pairs.DIL and DIW were regressed on the MOL, SIM, d2, H0and PIC. Results showed MOL of five SSR loci(12, 17, 24, 81 and 85) and SIM of five loci(17, 21, 24, 81and 85) all shared significant positive correlation with DIL(r=0.482, P=0.035 and r=0.479, P=0.035, respectively); H0of six SSR loci(11, 17, 21, 24, 26 and 85) had significant positive correlation with DIW(r=0.551, P=0.017); PIC of two SSR loci(9and 26) had significant positive(r=0.519, P=0.024) correlation with DIL, while that and of four loci(17, 24, 27 and 85) had significant negative correlation(r=-0.519,P=0.024), with DIL. This present study suggested that filial growth expression could be predicted by using molecular related indexes in turbot breeding practice, and the accuracy of prediction depends on more SSR loci, especially associated with QTL. 展开更多
关键词 TURBOT MICROSATELLITE Molecular related indexes Heterosis prediction
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