目的:通过氯化锂–毛果芸香碱建立大鼠癫痫持续状态模型,探究癫痫持续状态大鼠模型中不同时间点VEGF的表达变化,并探讨蒙药朝伦雄胡-5对VEGF表达的影响。方法:第一部分:选取90只6~8周龄健康雄性SD大鼠,随机分为造模成功组、给药对照组...目的:通过氯化锂–毛果芸香碱建立大鼠癫痫持续状态模型,探究癫痫持续状态大鼠模型中不同时间点VEGF的表达变化,并探讨蒙药朝伦雄胡-5对VEGF表达的影响。方法:第一部分:选取90只6~8周龄健康雄性SD大鼠,随机分为造模成功组、给药对照组和生理盐水对照组,每组30只。造模成功组与给药对照组通过腹腔注射氯化锂–毛果芸香碱制作大鼠癫痫持续状态模型,生理盐水对照组给予同等剂量的生理盐水处理,造模完成后应用qRT-PCR和Western blot方法检测VEGF转录水平及蛋白水平的表达情况。第二部分:选取40只6~8周龄健康雄性SD大鼠,随机分为蒙药组和对照组。制作癫痫大鼠模型前3 d,蒙药组给予蒙药朝伦雄胡-5灌胃处理,对照组给予同等剂量的生理盐水灌胃,3 d后对蒙药组和对照组通过腹腔注射氯化锂–毛果芸香碱制作大鼠癫痫持续状态模型。造模完成后应用qRT-PCR和Western blot方法检测VEGF转录水平及蛋白水平的表达情况。结果:第一部分:造模成功组VEGF mRNA表达较给药对照组和生理盐水对照组相比显著升高(P Objective: To explore the changes in VEGF expression at different time points in the rat model of status epilepticus by lithium chloride-rutin and explore the effect of Mongolian medicine Zhaolun Xionghu-5 on VEGF expression. Methods: Part I: 90 healthy male rats aged 6~8 weeks were randomly divided into successful modeling group, drug administration control group and normal saline control group, with 30 rats in each group. The rats in the successful modeling group and the control group were intraperitoneally injected with lithium chloride-rutin to establish the rat model of status epilepticus. The rats in the normal saline control group were treated with the same dose of normal saline. After the modeling, qRT-PCR and Western blot were used to detect the expression of VEGF transcription and protein levels. Part II: 40 healthy male rats aged 6~8 weeks were randomly divided into Mongolian medicine group and control group. Three days before the establishment of the rat model of status epilepticus, the Mongolian medicine group was given Mongolian medicine Zholun Xionghu-5 by gavage, and the control group was given the same dose of normal saline by gavage. Three days later, the Mongolian medicine group and the control group were intraperitoneally injected with lithium chloride-rutin to establish the rat model of status epilepticus. After the modeling, qRT-PCR and Western blot were used to detect the expression of VEGF transcription and protein levels. Results: Part I: The mRNA expression of VEGF in the successful modeling group was significantly higher than that in the administration control group and the normal saline control group (P < 0.05). There was no significant difference in VEGF mRNA expression between the administration control group and the normal saline control group. Moreover, VEGF mRNA in the successful modeling group increased gradually with the increase of time, while VEGF mRNA in the administration control group and normal saline control group did not change with time. Compared with the other two groups, the protein expression of VEGF in the successful modeling group was significantly increased (P < 0.05). There was no significant difference in VEGF protein expression between the administration control group and the normal saline control group. Moreover, the changes in VEGF protein in the three groups were no time dependence. Part II: Compared with the control group, the expression of VEGF mRNA in the Mongolian medicine group was significantly decreased (P < 0.05), and with the growth of time, VEGF continued to decrease;the expression of VEGF protein in Mongolian medicine group was significantly lower than that in successful modeling group (P < 0.05), but there was no time dependence. Conclusion: 1) VEGF mRNA expression was increased in hippocampus of rats with status epilepticus induced by lithium chloride-rutin. 2) The expression of VEGF protein in hippocampus of rats with status epilepticus induced by lithium chlorine-rutin was increased. 3) Mongolian medicine Zhaolun Xionghu-5 can induce the decrease of VEGF mRNA expression in hippocampus of rats with status epilepticus, and it decreases with time. 4) Mongolian medicine Zhaolun Xionghu-5 can partially inhibit elevated VEGF expression at the protein level in the model of status epilepticus.展开更多
目的探讨全蝎醇提物(Ethanol Extracts of Scorpion,EES)对大鼠癫痫持续状态(SE)后海马神经元凋亡的影响。方法建立氯化锂-匹罗卡品癫痫持续状态(Lithium-Pilocarpine induced status epilepticus,Li-Pilo SE)模型。使用TUNEL技术观察...目的探讨全蝎醇提物(Ethanol Extracts of Scorpion,EES)对大鼠癫痫持续状态(SE)后海马神经元凋亡的影响。方法建立氯化锂-匹罗卡品癫痫持续状态(Lithium-Pilocarpine induced status epilepticus,Li-Pilo SE)模型。使用TUNEL技术观察正常对照组、Li-Pilo SE模型组、丙戊酸钠组(VPA)和EES低(L)、中(M)、高(H)剂量组大鼠SE后6h、24h、48h、72h和7d海马CA1区和CA3区TUNEL阳性细胞的动态变化,并进行组间比较。结果正常对照组未见TUNEL阳性细胞。造模各组大鼠SE后6h海马可见部分TUNEL阳性细胞,主要分布在CA1、CA3区,其中模型组和EES(L)组72h达高峰,而VPA、EES(M)和EES(H)组高峰提前到SE后48h,以后各组逐渐下降。VPA、EES(M)和EES(H)组SE后各观察时间点TUNEL阳性细胞数较模型组极显著减少(P<0.01),而EES(L)组除个别时间点外,与模型组比较无统计学差异(P>0.05),其中VPA和EES(H)组各时间点TUNEL阳性细胞数减少较EES(M)组(P<0.01,和P<0.05)及EES(L)组(P<0.01,和P<0.05)更显著,EES(M)组各时间点减少又较EES(L)组更显著(P<0.01),而VPA与EES(H)组TUNEL阳性细胞数在各时间点无统计学差异(P>0.05)。结论EES能防止Li-Pilo SE大鼠海马神经元凋亡,并呈明显的量-效关系,高剂量EES抗凋亡作用与VPA相近。展开更多
文摘目的:通过氯化锂–毛果芸香碱建立大鼠癫痫持续状态模型,探究癫痫持续状态大鼠模型中不同时间点VEGF的表达变化,并探讨蒙药朝伦雄胡-5对VEGF表达的影响。方法:第一部分:选取90只6~8周龄健康雄性SD大鼠,随机分为造模成功组、给药对照组和生理盐水对照组,每组30只。造模成功组与给药对照组通过腹腔注射氯化锂–毛果芸香碱制作大鼠癫痫持续状态模型,生理盐水对照组给予同等剂量的生理盐水处理,造模完成后应用qRT-PCR和Western blot方法检测VEGF转录水平及蛋白水平的表达情况。第二部分:选取40只6~8周龄健康雄性SD大鼠,随机分为蒙药组和对照组。制作癫痫大鼠模型前3 d,蒙药组给予蒙药朝伦雄胡-5灌胃处理,对照组给予同等剂量的生理盐水灌胃,3 d后对蒙药组和对照组通过腹腔注射氯化锂–毛果芸香碱制作大鼠癫痫持续状态模型。造模完成后应用qRT-PCR和Western blot方法检测VEGF转录水平及蛋白水平的表达情况。结果:第一部分:造模成功组VEGF mRNA表达较给药对照组和生理盐水对照组相比显著升高(P Objective: To explore the changes in VEGF expression at different time points in the rat model of status epilepticus by lithium chloride-rutin and explore the effect of Mongolian medicine Zhaolun Xionghu-5 on VEGF expression. Methods: Part I: 90 healthy male rats aged 6~8 weeks were randomly divided into successful modeling group, drug administration control group and normal saline control group, with 30 rats in each group. The rats in the successful modeling group and the control group were intraperitoneally injected with lithium chloride-rutin to establish the rat model of status epilepticus. The rats in the normal saline control group were treated with the same dose of normal saline. After the modeling, qRT-PCR and Western blot were used to detect the expression of VEGF transcription and protein levels. Part II: 40 healthy male rats aged 6~8 weeks were randomly divided into Mongolian medicine group and control group. Three days before the establishment of the rat model of status epilepticus, the Mongolian medicine group was given Mongolian medicine Zholun Xionghu-5 by gavage, and the control group was given the same dose of normal saline by gavage. Three days later, the Mongolian medicine group and the control group were intraperitoneally injected with lithium chloride-rutin to establish the rat model of status epilepticus. After the modeling, qRT-PCR and Western blot were used to detect the expression of VEGF transcription and protein levels. Results: Part I: The mRNA expression of VEGF in the successful modeling group was significantly higher than that in the administration control group and the normal saline control group (P < 0.05). There was no significant difference in VEGF mRNA expression between the administration control group and the normal saline control group. Moreover, VEGF mRNA in the successful modeling group increased gradually with the increase of time, while VEGF mRNA in the administration control group and normal saline control group did not change with time. Compared with the other two groups, the protein expression of VEGF in the successful modeling group was significantly increased (P < 0.05). There was no significant difference in VEGF protein expression between the administration control group and the normal saline control group. Moreover, the changes in VEGF protein in the three groups were no time dependence. Part II: Compared with the control group, the expression of VEGF mRNA in the Mongolian medicine group was significantly decreased (P < 0.05), and with the growth of time, VEGF continued to decrease;the expression of VEGF protein in Mongolian medicine group was significantly lower than that in successful modeling group (P < 0.05), but there was no time dependence. Conclusion: 1) VEGF mRNA expression was increased in hippocampus of rats with status epilepticus induced by lithium chloride-rutin. 2) The expression of VEGF protein in hippocampus of rats with status epilepticus induced by lithium chlorine-rutin was increased. 3) Mongolian medicine Zhaolun Xionghu-5 can induce the decrease of VEGF mRNA expression in hippocampus of rats with status epilepticus, and it decreases with time. 4) Mongolian medicine Zhaolun Xionghu-5 can partially inhibit elevated VEGF expression at the protein level in the model of status epilepticus.
文摘目的探讨全蝎醇提物(Ethanol Extracts of Scorpion,EES)对大鼠癫痫持续状态(SE)后海马神经元凋亡的影响。方法建立氯化锂-匹罗卡品癫痫持续状态(Lithium-Pilocarpine induced status epilepticus,Li-Pilo SE)模型。使用TUNEL技术观察正常对照组、Li-Pilo SE模型组、丙戊酸钠组(VPA)和EES低(L)、中(M)、高(H)剂量组大鼠SE后6h、24h、48h、72h和7d海马CA1区和CA3区TUNEL阳性细胞的动态变化,并进行组间比较。结果正常对照组未见TUNEL阳性细胞。造模各组大鼠SE后6h海马可见部分TUNEL阳性细胞,主要分布在CA1、CA3区,其中模型组和EES(L)组72h达高峰,而VPA、EES(M)和EES(H)组高峰提前到SE后48h,以后各组逐渐下降。VPA、EES(M)和EES(H)组SE后各观察时间点TUNEL阳性细胞数较模型组极显著减少(P<0.01),而EES(L)组除个别时间点外,与模型组比较无统计学差异(P>0.05),其中VPA和EES(H)组各时间点TUNEL阳性细胞数减少较EES(M)组(P<0.01,和P<0.05)及EES(L)组(P<0.01,和P<0.05)更显著,EES(M)组各时间点减少又较EES(L)组更显著(P<0.01),而VPA与EES(H)组TUNEL阳性细胞数在各时间点无统计学差异(P>0.05)。结论EES能防止Li-Pilo SE大鼠海马神经元凋亡,并呈明显的量-效关系,高剂量EES抗凋亡作用与VPA相近。
