[ Objective] The study aimed to reveal the biological nitrogen fixation capacity by sugarcane from Brazil under the ecological conditions of Guangxi, and to provide reference for study on the biological nitrogen fixat...[ Objective] The study aimed to reveal the biological nitrogen fixation capacity by sugarcane from Brazil under the ecological conditions of Guangxi, and to provide reference for study on the biological nitrogen fixation capacity by sugarcane and related generalization and application. [ Method] The ^15N isotopic fertilizer was solely applied on plants of three sugarcane cultivars planted in greenhouse with no other fertilizer forms applied, meanwhile virus-free stem seedling was regarded as control, to measure their biological nitrogen fixation capacity using ^15N isotope. [ Result ] The nitrogen fixation rate of B8 from Brazil reached 26.91%, while Guitang 11 and RIC16 presented no or poor nitrogen fixation capacity. [ Conclusion] The sugarcane eultivar B8 from Brazil showed some nitrogen fixation capacity under the ecological conditions of Guangxi.展开更多
The objective of this study was to determine the efficiency of biological nitrogen fixation （BNF） of local Bradyrhizobium isolates in soil of various fertility levels using 15N dilution technique. Local isolates wer...The objective of this study was to determine the efficiency of biological nitrogen fixation （BNF） of local Bradyrhizobium isolates in soil of various fertility levels using 15N dilution technique. Local isolates were obtained from cowpea rhizosphere in fields of different Iraqi provinces. Six isolates were selected in this study, which was conducted as a pot experiment under greenhouse conditions. Effects of the following fertility levels were evaluated： at F1, 0 mg N, P and K was added; at F2, 25 mg N/kg soil, 10 mg P/kg soil and 25 mg K/kg soil were added, respectively; the other two levels were F3 at which 50, 20 and 50 mg/kg soil and at F4 75, 30 and 75 mg/kg soil for N, P and K, respectively, were added. Urea, labeled with 15N 10% access atom （aa）, was used as a source of N. The highest BNF was observed under the lowest fertility level, i.e., F1. BNF across all isolates was markedly decreased with the increase of nutrient application to soil, being totally eliminated at the highest fertility level F4. Numbers of nodules per plant root of all isolates were the least under the zero nutrients application and the highest nodules number were found under the highest levels of N, P and K application. Number of nodules does not necessarily reflect the best BNF efficiency of all isolates. However, fertility levels were of significant effect on average nodule number of all isolates. The lowest plant dry weight was under the first fertility level F1 irrelevant of Rhizobium isolates. In general, the highest plant dry weight was under the second soil fertility level F2.展开更多
基金National Natural Science Foundation of China (3026005430660085)+1 种基金Key Project of Guangxi Academy of Agricultural Sciences (2004002)Natural Science Foundation in Guangxi Zhuang Autonomous Region (0639011)~~
文摘[ Objective] The study aimed to reveal the biological nitrogen fixation capacity by sugarcane from Brazil under the ecological conditions of Guangxi, and to provide reference for study on the biological nitrogen fixation capacity by sugarcane and related generalization and application. [ Method] The ^15N isotopic fertilizer was solely applied on plants of three sugarcane cultivars planted in greenhouse with no other fertilizer forms applied, meanwhile virus-free stem seedling was regarded as control, to measure their biological nitrogen fixation capacity using ^15N isotope. [ Result ] The nitrogen fixation rate of B8 from Brazil reached 26.91%, while Guitang 11 and RIC16 presented no or poor nitrogen fixation capacity. [ Conclusion] The sugarcane eultivar B8 from Brazil showed some nitrogen fixation capacity under the ecological conditions of Guangxi.
文摘The objective of this study was to determine the efficiency of biological nitrogen fixation （BNF） of local Bradyrhizobium isolates in soil of various fertility levels using 15N dilution technique. Local isolates were obtained from cowpea rhizosphere in fields of different Iraqi provinces. Six isolates were selected in this study, which was conducted as a pot experiment under greenhouse conditions. Effects of the following fertility levels were evaluated： at F1, 0 mg N, P and K was added; at F2, 25 mg N/kg soil, 10 mg P/kg soil and 25 mg K/kg soil were added, respectively; the other two levels were F3 at which 50, 20 and 50 mg/kg soil and at F4 75, 30 and 75 mg/kg soil for N, P and K, respectively, were added. Urea, labeled with 15N 10% access atom （aa）, was used as a source of N. The highest BNF was observed under the lowest fertility level, i.e., F1. BNF across all isolates was markedly decreased with the increase of nutrient application to soil, being totally eliminated at the highest fertility level F4. Numbers of nodules per plant root of all isolates were the least under the zero nutrients application and the highest nodules number were found under the highest levels of N, P and K application. Number of nodules does not necessarily reflect the best BNF efficiency of all isolates. However, fertility levels were of significant effect on average nodule number of all isolates. The lowest plant dry weight was under the first fertility level F1 irrelevant of Rhizobium isolates. In general, the highest plant dry weight was under the second soil fertility level F2.
