We investigated the distribution of four enzymes involved in the immune response of Apostichopus japonicus. We collected samples of the tentacles, papillate podium, integument, respiratory tree, and digestive tract an...We investigated the distribution of four enzymes involved in the immune response of Apostichopus japonicus. We collected samples of the tentacles, papillate podium, integument, respiratory tree, and digestive tract and stained them for acid phosphatase （ACP）, alkaline phosphatase （AKP）, non-specific esterase （NSE） and peroxidase （POD） activity. The distribution and content of ACE AKP, NSE, and POD differed among the tissues. The coelomic epithelium of the tentacle, papillate podium, and integument and the mucous layer of respiratory tree were positive for ACP activity. The coelomic epithelium and cuticular layer of the tentacle, papillate podium, and integument and the mucous layer and tunica externa of the respiratory tree and digestive tract stained positive or weakly positive for AKP activity. Almost all the epithelial tissues stained positive, strongly positive, or very strongly positive for NSE activity. The cuticular layer of the tentacle and integument and the mucous layer, tunica submucosa, and tunica externa of the respiratory tree and digestive tract stained positive for POD activity. We hypothesize that these enzymes play a role in the immune response in A. japonicus.展开更多
In the traditional Intemet Protocol （IP） architecture, there is an overload of IP sermntic problems. Existing solutions focused mainly on the infrastructure for the fixed network, and there is a lack of support for ...In the traditional Intemet Protocol （IP） architecture, there is an overload of IP sermntic problems. Existing solutions focused mainly on the infrastructure for the fixed network, and there is a lack of support for Mobile Ad Hoc Networks （MANETs）. To improve scalability, a routing protocol for MANETs is presented based on a locator named Tree-structure Locator Distance Vector （TLDV）. The hard core of this routing method is the identifier/locator split by the Distributed Hash Table （DHT） method, which provides a scalable routing service. The node locator indicates its relative location in the network and should be updated whenever topology changes, kocator space ks organized as a tree-structure, and the basic routing operation of the TLDV protocol is presented. TLDV protocol is compared to some classical routing protocols for MANETs on the NS2 platform Results show that TLDV has better scalability. Key words：展开更多
基金Supported by the Special Financed Project from the Basic Public Scientific Research Expenses of Central Scientific Institutes (No GY02-2007B03)
文摘We investigated the distribution of four enzymes involved in the immune response of Apostichopus japonicus. We collected samples of the tentacles, papillate podium, integument, respiratory tree, and digestive tract and stained them for acid phosphatase （ACP）, alkaline phosphatase （AKP）, non-specific esterase （NSE） and peroxidase （POD） activity. The distribution and content of ACE AKP, NSE, and POD differed among the tissues. The coelomic epithelium of the tentacle, papillate podium, and integument and the mucous layer of respiratory tree were positive for ACP activity. The coelomic epithelium and cuticular layer of the tentacle, papillate podium, and integument and the mucous layer and tunica externa of the respiratory tree and digestive tract stained positive or weakly positive for AKP activity. Almost all the epithelial tissues stained positive, strongly positive, or very strongly positive for NSE activity. The cuticular layer of the tentacle and integument and the mucous layer, tunica submucosa, and tunica externa of the respiratory tree and digestive tract stained positive for POD activity. We hypothesize that these enzymes play a role in the immune response in A. japonicus.
基金Acknowledgements This work was supported by the Hi-Tech Research and Development Program of China under Grant No.2007AA01Z407 the Co-Funding Project of Beijing Municipal education Commission under Grant No.JD100060630+3 种基金 National Foundation Research Project the National Natural Science Foundation Project under Grant No. 61170295 the Project of Aeronautical Science Foundation of China under Caant No.2011ZC51024 and the Fundamental Research Funds for the Central Universities.
文摘In the traditional Intemet Protocol （IP） architecture, there is an overload of IP sermntic problems. Existing solutions focused mainly on the infrastructure for the fixed network, and there is a lack of support for Mobile Ad Hoc Networks （MANETs）. To improve scalability, a routing protocol for MANETs is presented based on a locator named Tree-structure Locator Distance Vector （TLDV）. The hard core of this routing method is the identifier/locator split by the Distributed Hash Table （DHT） method, which provides a scalable routing service. The node locator indicates its relative location in the network and should be updated whenever topology changes, kocator space ks organized as a tree-structure, and the basic routing operation of the TLDV protocol is presented. TLDV protocol is compared to some classical routing protocols for MANETs on the NS2 platform Results show that TLDV has better scalability. Key words：
