传统的表达性写作是指邀请参与者进行自由写作,并不给予反馈。在后续研究与应用中,研究者逐渐将表达性写作与不同的心理治疗理论或不同变量结合起来,进行结构化表达性写作或主题写作,这使得表达性写作更具针对性。本文欲在介绍表达性写...传统的表达性写作是指邀请参与者进行自由写作,并不给予反馈。在后续研究与应用中,研究者逐渐将表达性写作与不同的心理治疗理论或不同变量结合起来,进行结构化表达性写作或主题写作,这使得表达性写作更具针对性。本文欲在介绍表达性写作可能的作用机制的基础上,结合心理动力学、认知行为治疗、存在主义心理治疗、积极心理治疗等理论,提出更灵活的表达性写作干预方案,以帮助人们将表达性写作应用于心理咨询的自助与助人过程中,提高人的心理健康水平。Traditional expressive writing means inviting participants to engage in free writing without providing any feedback. In subsequent research and applications, researchers gradually combined expressive writing with different psychotherapeutic theories or various variables to conduct structured expressive writing or thematic writing. This has made expressive writing more targeted. Based on introducing the possible mechanisms of expressive writing, this paper aims to integrate theories such as psychodynamic theory, cognitive behavioral therapy, existential psychotherapy, and positive psychotherapy, and propose a more flexible expressive writing intervention plan. This is to assist people in applying expressive writing to the self-help and helping-others processes in psychological counseling, thereby enhancing people’s mental health.展开更多
[Objective] The research aimed to induce the expression of FMDV structural protein VP1 in E.coli and purify the protein,then detect the activity.[Method] The fragment coding VP1 was amplified by PCR and doubly digeste...[Objective] The research aimed to induce the expression of FMDV structural protein VP1 in E.coli and purify the protein,then detect the activity.[Method] The fragment coding VP1 was amplified by PCR and doubly digested with BamH Ⅰ and XhoⅠ,then cloned into expression vector pGEX-4T-1 and pPROExHTb respectively to get recombinant plasmid pGEX-4T-1-VP1 and pPROExHTb-VP1.The recombinant plasmid pGEX-4T-1-VP1 and pPROExHTb-VP1 was transformed into E.coli BL21(DE3)and induced by IPTG,fusion protein was identifie...展开更多
结论先行是逆向思维的主要思维方向。以人教版(2019年版)高中《英语》必修三Unit 1Festivals and celebrations为例,说明基于逆向思维的高中英语议论文写作实践。认为在高中英语议论文写作中,教师可以从逆向调整教材内容、逆向确立写作...结论先行是逆向思维的主要思维方向。以人教版(2019年版)高中《英语》必修三Unit 1Festivals and celebrations为例,说明基于逆向思维的高中英语议论文写作实践。认为在高中英语议论文写作中,教师可以从逆向调整教材内容、逆向确立写作立意、逆向设定习作结构、逆向遣词造句等方面,帮助学生完成论点链有序、论据丰实、说服力强的写作,促进思维与表达的共生共进。展开更多
Metalloproteinases have a critical role in a broad spectrum of cellular processesranging from the break-down of extracellular matrix to the processing of signaltransduction-related proteins.These hydrolytic functions ...Metalloproteinases have a critical role in a broad spectrum of cellular processesranging from the break-down of extracellular matrix to the processing of signaltransduction-related proteins.These hydrolytic functions underlie a variety of mechanisms related to developmental processes as well as disease states.Structural analysis of metalloproteinases from both invertebrate and vertebrate species indicates that these enzymes are highly conserved and arose early during metazoan evolution.In this regard,studies from vari-ous laboratories have reported that a number of classes of metalloproteinases are found in hydra,a member of Cnidaria,the second oldest of existing animal phyla.These studies demonstrate that the hydra genome contains at least three classes of metalloproteinases to include members of the 1)astacin class,2)matrix met-alloproteinase class,and 3)neprilysin class.Functional studies indicate that these metalloproteinases play diverse and important roles in hydra morphogenesis and cell differentiation as well as specialized functions in adult polyps.This article will review the structure,expression,and function of these metalloproteinases in hydra.展开更多
文摘传统的表达性写作是指邀请参与者进行自由写作,并不给予反馈。在后续研究与应用中,研究者逐渐将表达性写作与不同的心理治疗理论或不同变量结合起来,进行结构化表达性写作或主题写作,这使得表达性写作更具针对性。本文欲在介绍表达性写作可能的作用机制的基础上,结合心理动力学、认知行为治疗、存在主义心理治疗、积极心理治疗等理论,提出更灵活的表达性写作干预方案,以帮助人们将表达性写作应用于心理咨询的自助与助人过程中,提高人的心理健康水平。Traditional expressive writing means inviting participants to engage in free writing without providing any feedback. In subsequent research and applications, researchers gradually combined expressive writing with different psychotherapeutic theories or various variables to conduct structured expressive writing or thematic writing. This has made expressive writing more targeted. Based on introducing the possible mechanisms of expressive writing, this paper aims to integrate theories such as psychodynamic theory, cognitive behavioral therapy, existential psychotherapy, and positive psychotherapy, and propose a more flexible expressive writing intervention plan. This is to assist people in applying expressive writing to the self-help and helping-others processes in psychological counseling, thereby enhancing people’s mental health.
基金Supported by National Supporting Plan(2006BAD06A14)Transgenic Major Projects(2008ZX08011-004)~~
文摘[Objective] The research aimed to induce the expression of FMDV structural protein VP1 in E.coli and purify the protein,then detect the activity.[Method] The fragment coding VP1 was amplified by PCR and doubly digested with BamH Ⅰ and XhoⅠ,then cloned into expression vector pGEX-4T-1 and pPROExHTb respectively to get recombinant plasmid pGEX-4T-1-VP1 and pPROExHTb-VP1.The recombinant plasmid pGEX-4T-1-VP1 and pPROExHTb-VP1 was transformed into E.coli BL21(DE3)and induced by IPTG,fusion protein was identifie...
文摘结论先行是逆向思维的主要思维方向。以人教版(2019年版)高中《英语》必修三Unit 1Festivals and celebrations为例,说明基于逆向思维的高中英语议论文写作实践。认为在高中英语议论文写作中,教师可以从逆向调整教材内容、逆向确立写作立意、逆向设定习作结构、逆向遣词造句等方面,帮助学生完成论点链有序、论据丰实、说服力强的写作,促进思维与表达的共生共进。
文摘Metalloproteinases have a critical role in a broad spectrum of cellular processesranging from the break-down of extracellular matrix to the processing of signaltransduction-related proteins.These hydrolytic functions underlie a variety of mechanisms related to developmental processes as well as disease states.Structural analysis of metalloproteinases from both invertebrate and vertebrate species indicates that these enzymes are highly conserved and arose early during metazoan evolution.In this regard,studies from vari-ous laboratories have reported that a number of classes of metalloproteinases are found in hydra,a member of Cnidaria,the second oldest of existing animal phyla.These studies demonstrate that the hydra genome contains at least three classes of metalloproteinases to include members of the 1)astacin class,2)matrix met-alloproteinase class,and 3)neprilysin class.Functional studies indicate that these metalloproteinases play diverse and important roles in hydra morphogenesis and cell differentiation as well as specialized functions in adult polyps.This article will review the structure,expression,and function of these metalloproteinases in hydra.
