AIM: To introduce a computer-aided morphometric method for quantifying the necro-inflammatory phase in liver biopsy specimens using fractal geometry and Delaunay's triangulation.METHODS: Two-micrometer thick biops...AIM: To introduce a computer-aided morphometric method for quantifying the necro-inflammatory phase in liver biopsy specimens using fractal geometry and Delaunay's triangulation.METHODS: Two-micrometer thick biopsy sections taken from 78 chronic hepatitis C virus-infected patients were immunohistochemically treated to identify the inflammatory cells. An automatic computer-aided image analysis system was used to define the inflammatory cell network defined on the basis of Delaunay's triangulation,and the inflammatory cells were geometrically classified as forming a cluster (an aggregation of a minimum of three cells) or as being irregularly distributed within the tissue. The phase of inflammatory activity was estimated using Hurst's exponent.RESULTS: The proposed automatic method was rapid and objective. It could not only provide rigorous results expressed by scalar numbers, but also allow the state of the whole organ to be represented by Hurst's exponent with an error of no more than 12%.CONCLUSION: The availability of rigorous metrical measures and the reasonable representativeness of the status of the organ as a whole raise the question as to whether the indication for hepatic biopsy should be revised by establishing clear rules concerning the contraindications suggested by its invasiveness and subjective interpretation.展开更多
Doxorubicin is associated with adverse effects on organs like liver. This study was done to find the effects of doxorubicin on liver of wistar albino rats. Sixty healthy wistar albino rats were taken for the study. Th...Doxorubicin is associated with adverse effects on organs like liver. This study was done to find the effects of doxorubicin on liver of wistar albino rats. Sixty healthy wistar albino rats were taken for the study. The rats were randomly divided in to 4 groups （2 experimental groups; group AI and A2, and 2 control groups;group Bland B2 each containing 16 rats）. The experimental groups were given a single dose of doxorubicin i.e. 10 mg/kg body weight intraperitoneally and sacrificed after 7 days and 14 days for each group. Rats under control groups were given a single intraperitoneal dose of 2.5 ml/kg body weight normal saline and sacrificed with their respective experimental groups. A significant difference （p 〈 0.001） and （p = 0.005） in final body weight was observed among group A1 experimental and BI control rats, and among group A2 experimental and B2 control rats. As compared to group B2 control, group A2 experimental rats had significantly （p = 0.043） lower liver weight. Diameter ofhepatocyte （p 〈 0.001 for both groups） and nucleus （p=0.004 for group A1 and control BI, and p 〈 0.001 for group A2 and control B2） was significantly higher in experimental rats as compared to their respective controls. Cross-sections of the liver of both control groups had normal architecture. However, there was progressive destruction of liver architecture across the experimental groups. Doxorubicin causes the disruption of normal architecture of liver in rats. Thus care needs to be taken during doxorubicin chemotherapy to minimize effects on river.展开更多
基金Supported by the "Michele Rodriguez" Foundation, Institute for Quantitative Measures in Medicine, Milan, Italy
文摘AIM: To introduce a computer-aided morphometric method for quantifying the necro-inflammatory phase in liver biopsy specimens using fractal geometry and Delaunay's triangulation.METHODS: Two-micrometer thick biopsy sections taken from 78 chronic hepatitis C virus-infected patients were immunohistochemically treated to identify the inflammatory cells. An automatic computer-aided image analysis system was used to define the inflammatory cell network defined on the basis of Delaunay's triangulation,and the inflammatory cells were geometrically classified as forming a cluster (an aggregation of a minimum of three cells) or as being irregularly distributed within the tissue. The phase of inflammatory activity was estimated using Hurst's exponent.RESULTS: The proposed automatic method was rapid and objective. It could not only provide rigorous results expressed by scalar numbers, but also allow the state of the whole organ to be represented by Hurst's exponent with an error of no more than 12%.CONCLUSION: The availability of rigorous metrical measures and the reasonable representativeness of the status of the organ as a whole raise the question as to whether the indication for hepatic biopsy should be revised by establishing clear rules concerning the contraindications suggested by its invasiveness and subjective interpretation.
文摘Doxorubicin is associated with adverse effects on organs like liver. This study was done to find the effects of doxorubicin on liver of wistar albino rats. Sixty healthy wistar albino rats were taken for the study. The rats were randomly divided in to 4 groups （2 experimental groups; group AI and A2, and 2 control groups;group Bland B2 each containing 16 rats）. The experimental groups were given a single dose of doxorubicin i.e. 10 mg/kg body weight intraperitoneally and sacrificed after 7 days and 14 days for each group. Rats under control groups were given a single intraperitoneal dose of 2.5 ml/kg body weight normal saline and sacrificed with their respective experimental groups. A significant difference （p 〈 0.001） and （p = 0.005） in final body weight was observed among group A1 experimental and BI control rats, and among group A2 experimental and B2 control rats. As compared to group B2 control, group A2 experimental rats had significantly （p = 0.043） lower liver weight. Diameter ofhepatocyte （p 〈 0.001 for both groups） and nucleus （p=0.004 for group A1 and control BI, and p 〈 0.001 for group A2 and control B2） was significantly higher in experimental rats as compared to their respective controls. Cross-sections of the liver of both control groups had normal architecture. However, there was progressive destruction of liver architecture across the experimental groups. Doxorubicin causes the disruption of normal architecture of liver in rats. Thus care needs to be taken during doxorubicin chemotherapy to minimize effects on river.
