目的:探究窄谱中波紫外线联合0.1%他克莫司软膏治疗白癜风患者疗效及对皮损面积和色素积分的影响。方法:将笔者医院皮肤科于2020年1月-2023年1月收治的96例门诊患者纳为研究对象,根据随机数字法将所有患者分为对照组与观察组,每组各48例...目的:探究窄谱中波紫外线联合0.1%他克莫司软膏治疗白癜风患者疗效及对皮损面积和色素积分的影响。方法:将笔者医院皮肤科于2020年1月-2023年1月收治的96例门诊患者纳为研究对象,根据随机数字法将所有患者分为对照组与观察组,每组各48例,给予对照组患者外涂0.1%他克莫司软膏治疗;观察组在对照组治疗基础上联合窄谱中波紫外线治疗。对比两组患者整体疗效,治疗前后皮损面积(Wood灯下皮损评估)与色素积分、不同部位严重性指数(Psoriasis area and severity index,PASI)与白癜风面积指数(Vitiligo area and severity index,VASI),不良反应发生情况及复发率。结果:观察组总有效率(97.92%)显著高于对照组(85.42%)(P<0.05);治疗后,两组患者皮损面积显著降低(P<0.05),且观察组显著低于对照组(P<0.05);治疗后,两组色素积分显著上升(P<0.05),且观察组显著高于对照组(P<0.05);治疗后,观察组各部位(躯干、四肢、面部)PASI与VASI均显著低于对照组(P<0.05);两组患者治疗期间不良反应发生率[观察组(16.67%) vs.对照组(10.42%)]与复发率[观察组(6.25%)vs.对照组(8.33%)]差异无统计学意义(P>0.05)。结论:窄谱中波紫外线联合0.1%他克莫司软膏可显著改善白癜风患者病情,缓解皮肤损伤,增加皮肤色素沉着,安全有效,值得临床推广。展开更多
Sedimentary pigments are useful proxy indicators of phytoplankton biomass, community structure, primary productivity and human influence in lacustrine and oceanic ecosystems. Pigments are relatively labile due to thei...Sedimentary pigments are useful proxy indicators of phytoplankton biomass, community structure, primary productivity and human influence in lacustrine and oceanic ecosystems. Pigments are relatively labile due to their complex chemical structures, which makes the extraction and analysis of sedimentary pigments challenging. In addition, it is important to select appropriate methods to study sedimentary pigments in regions which lack previous investigations. In this study, we adopted the L9（34） orthogonal design to develop methods of extraction and HPLC （high performance liquid chromatography） analysis of sedimentary pigments at two lakes on the Tibetan Plateau： meromictic lake-Dagze Co and dimictic lake-Jiang Co. The orthogonal design comprises 9 combinations of various parameters for extraction and HPLC analysis. The results show that the type and volume of solvent are the most important factors for pigment extraction, and the mobile phase and column selection are the most important for HPLC analysis. For the study sites, we found that the best methods to extract sedimentary pigments are as follows： the use of a mixture of acetone：methanol：water （80： 15：5, v：v：v） as the extraction solvent; sol- vent/sample ratio of 10 mL/g; sonication for 30 s and standing extraction for 6 h. The best methods for HPLC analysis are as follows： Zorbax Eclipse plus C8 column with mobile phase A, methanol：acetonitrile：0.25 M aqueous pyridine （50： 25：25, v ： v ： v） and mobile phase B, methanol： acetonitrile： acetone （20： 60： 20, v： v： v）; pH of mobile phase A adjusted to 6 with acetic acid; and HPLC column temperature maintained at 40~C. The study provides an experimental basis for future investigations of past changes in primary productivity and the response of lake ecosystems to climate change and human activities on the Tibetan Plateau.展开更多
文摘目的:探究窄谱中波紫外线联合0.1%他克莫司软膏治疗白癜风患者疗效及对皮损面积和色素积分的影响。方法:将笔者医院皮肤科于2020年1月-2023年1月收治的96例门诊患者纳为研究对象,根据随机数字法将所有患者分为对照组与观察组,每组各48例,给予对照组患者外涂0.1%他克莫司软膏治疗;观察组在对照组治疗基础上联合窄谱中波紫外线治疗。对比两组患者整体疗效,治疗前后皮损面积(Wood灯下皮损评估)与色素积分、不同部位严重性指数(Psoriasis area and severity index,PASI)与白癜风面积指数(Vitiligo area and severity index,VASI),不良反应发生情况及复发率。结果:观察组总有效率(97.92%)显著高于对照组(85.42%)(P<0.05);治疗后,两组患者皮损面积显著降低(P<0.05),且观察组显著低于对照组(P<0.05);治疗后,两组色素积分显著上升(P<0.05),且观察组显著高于对照组(P<0.05);治疗后,观察组各部位(躯干、四肢、面部)PASI与VASI均显著低于对照组(P<0.05);两组患者治疗期间不良反应发生率[观察组(16.67%) vs.对照组(10.42%)]与复发率[观察组(6.25%)vs.对照组(8.33%)]差异无统计学意义(P>0.05)。结论:窄谱中波紫外线联合0.1%他克莫司软膏可显著改善白癜风患者病情,缓解皮肤损伤,增加皮肤色素沉着,安全有效,值得临床推广。
基金supported by National Key Basic Research Programs of China (Grant No. 2012CB956100)the National Natural Science Foundation of China (Grant Nos. 41072120 & 41321061)
文摘Sedimentary pigments are useful proxy indicators of phytoplankton biomass, community structure, primary productivity and human influence in lacustrine and oceanic ecosystems. Pigments are relatively labile due to their complex chemical structures, which makes the extraction and analysis of sedimentary pigments challenging. In addition, it is important to select appropriate methods to study sedimentary pigments in regions which lack previous investigations. In this study, we adopted the L9（34） orthogonal design to develop methods of extraction and HPLC （high performance liquid chromatography） analysis of sedimentary pigments at two lakes on the Tibetan Plateau： meromictic lake-Dagze Co and dimictic lake-Jiang Co. The orthogonal design comprises 9 combinations of various parameters for extraction and HPLC analysis. The results show that the type and volume of solvent are the most important factors for pigment extraction, and the mobile phase and column selection are the most important for HPLC analysis. For the study sites, we found that the best methods to extract sedimentary pigments are as follows： the use of a mixture of acetone：methanol：water （80： 15：5, v：v：v） as the extraction solvent; sol- vent/sample ratio of 10 mL/g; sonication for 30 s and standing extraction for 6 h. The best methods for HPLC analysis are as follows： Zorbax Eclipse plus C8 column with mobile phase A, methanol：acetonitrile：0.25 M aqueous pyridine （50： 25：25, v ： v ： v） and mobile phase B, methanol： acetonitrile： acetone （20： 60： 20, v： v： v）; pH of mobile phase A adjusted to 6 with acetic acid; and HPLC column temperature maintained at 40~C. The study provides an experimental basis for future investigations of past changes in primary productivity and the response of lake ecosystems to climate change and human activities on the Tibetan Plateau.
