期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
基于双特异纳米抗体FMDV血凝检测方法的建立
1
作者 杨利 张浩明 +3 位作者 乔绪稳 陈瑾 郑其升 程海卫 《江苏农业学报》 CSCD 北大核心 2024年第3期514-521,共8页
本研究利用基因工程方法将抗口蹄疫病毒(FMDV)纳米抗体Nb205和抗鸡醛化红细胞(cRBC)纳米抗体NbRBC48的基因片段连接,构建双特异纳米抗体Nb205-48,其相对分子质量为3.5×10^(4),能同时与FMDV和cRBC反应。利用Nb205-48成功建立了可用... 本研究利用基因工程方法将抗口蹄疫病毒(FMDV)纳米抗体Nb205和抗鸡醛化红细胞(cRBC)纳米抗体NbRBC48的基因片段连接,构建双特异纳米抗体Nb205-48,其相对分子质量为3.5×10^(4),能同时与FMDV和cRBC反应。利用Nb205-48成功建立了可用于FMDV抗原检测的血凝方法,该方法检测灵敏度为1μg/ml,与其他病毒无交叉反应,且批内和批间重复性好。分别采用血凝法和夹心ELISA方法对3批次FMDV抗原进行了检测,二者检测结果基本吻合。本研究建立了基于双特异纳米抗体Nb205-48的血凝检测方法,该方法具有较好的灵敏度、特异性和重复性,且简便、快捷、成本低,其检测结果与传统的FMDV定量检测方法的检测结果相关性好,为检测口蹄疫疫苗生产过程中FMDV抗原的含量提供了新方法。 展开更多
关键词 口蹄疫病毒 双特异性纳米抗体 血凝检测法
在线阅读 下载PDF
Comparative Studies on Detection of Antibodies against Infectious Bursal Disease Virus with Test Strips and Agar Gel Immunodiffusion Method
2
作者 张进良 张文通 +3 位作者 胡思顺 毕丁仁 王喜亮 肖运才 《Agricultural Science & Technology》 CAS 2012年第9期1826-1828,共3页
[Objective] This study aimed to compare the detection results of antibodies against infectious bursal disease virus with test strips and agar gel immunodiffusion method. [Method] Antibodies against infectious bursal d... [Objective] This study aimed to compare the detection results of antibodies against infectious bursal disease virus with test strips and agar gel immunodiffusion method. [Method] Antibodies against infectious bursal disease virus in chicken serum were detected using test strips developed in our laboratory, and the results were comparad^with that using traditional agar diffusion method. [Result] The comparative study of the two methods showed that the sensitivity of test strips was eight times over agar gel immunodiffusion; test strips showed higher detection rate in the deter- mination test of 216 clinical samples, with high specificity, easy preservation, and simple and rapid operation, thereby being more suitable for the monitoring of clinical antibodies. [Conclusion] Test strips could replace the existing serological methods, having great promotion and application value in antibody monitoring. 展开更多
关键词 Infectious bursal disease Agar gel immunodiffusion Test strips
在线阅读 下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部