AIM:To evaluate the association between Chlamydia pneumoniae (Cpn) infection and primary biliary cirrhosis (PBC). METHODS: CpnIq/G and IgM were determined by enzyme-linked immunosorbent assay (ELBA) in 41 well-establi...AIM:To evaluate the association between Chlamydia pneumoniae (Cpn) infection and primary biliary cirrhosis (PBC). METHODS: CpnIq/G and IgM were determined by enzyme-linked immunosorbent assay (ELBA) in 41 well-established PBC patients and two race-matched control groups (post-hepatitis cirrhosis, n = 70; healthy controls, n = 57). RESULTS: The mean level and seroprevalence of Cpn IgG in PBC group and post-hepatitis cirrhosis (PHC) group were significantly higher than those in healthy controls (46.8±43.4 RU/mL, 49.5±45.2 RU/mL vs28.3±32.7 RU/mL; 68.3%, 71.4%, 42.1%, respectively; P<0.05). There was a remarkably elevated seroprevalence of Cpn IgM in patients with PBC (22.0%) compared to the PHC and healthy control (HC) groups. For the PBC patients versus the HCs, the odds ratios (ORs) of the presence of Cpn IgG and IgM were 2.7 (95% CI 0.9-6.1) and 5.1 (95% CI 1.4-18.5), respectively. Though there was no correlation in the level of Cpn IgG with total IgG in sera of patients with PBC (r = -0.857, P = 0.344>0.05), Cpn IgM was related with the abnormally high concentrations of total IgM in PBC group. CONCLUSION: The results of this study do not support the hypothesis that infection with Chlamydia pneumoniae may be a triggering agent or even a causative agent in PBC, but suggest that Chlamydia pneumoniae infection probably contributes to the high level of IgM present in most patients with PBC.展开更多
Research data showed that syndromic approach could successfully manage gonococcal and chlamydial infections in males and syphilis and chancroid in males and females. However, low sensitivity, specificity and positive ...Research data showed that syndromic approach could successfully manage gonococcal and chlamydial infections in males and syphilis and chancroid in males and females. However, low sensitivity, specificity and positive predictive value were found in the syndromic management of vaginal discharge. It is recommended that the syndromic algorithm for management of vaginal discharge used when serving high-risk and symptomatic women.展开更多
Chlamydia, a human pathogen that mediates chronic, persistent and repeated infection progress, is capable of inflicting permanent scar formation. A typical reticular body is found in persistent infections. Chlamydial ...Chlamydia, a human pathogen that mediates chronic, persistent and repeated infection progress, is capable of inflicting permanent scar formation. A typical reticular body is found in persistent infections. Chlamydial Hsp 60, interferon(IFN) -gamma and other cytokines function in the course of repeated infection. Immunopathological factors mediate chronic infection.展开更多
To investigate the DNA sequence polymorphism of Chlamydia traehomatis ompl gene, urogenital samples were collected from 4 different cities in South China, DNA was extracted, and an approx- imately 980-bp-long fragment...To investigate the DNA sequence polymorphism of Chlamydia traehomatis ompl gene, urogenital samples were collected from 4 different cities in South China, DNA was extracted, and an approx- imately 980-bp-long fragment of the ompl gene was amplified by nested polymerase chain reaction （nPCR）. DNA sequence was determined, genotyping was performed by BLAST similarity search, and multiple alignment was performed with CLUSTAL X. Then a phylogenetie tree was constructed by Mega 3 software to illustrate the evolutionary relationships between clinical isolates and reference strains. Ninetysix specimens were sequenced, and 28 genetic variants were detected, among which E was the most prevalent genotype. The ompl gene was highly conserved for genotypes E and F, but appeared slightly less conserved for other genotypes, where the sequences displayed one to several nueleotide substitutions relative to the reference sequence. Phylogenetie tree showed that C. traehomatis serotypes were mainly divided into three clusters, according to previous grouping in the B, F-G, and C complexes, and the clinical isolates were highly related to the corresponding reference strains. It concluded that the ompl gene of the isolated C. traehomatis strains exhibited remarkable DNA sequence polymorphism, which can encourage for vaccine design and infection control.展开更多
基金Supported by the National Natural Science Foundation of China, No. 30300157
文摘AIM:To evaluate the association between Chlamydia pneumoniae (Cpn) infection and primary biliary cirrhosis (PBC). METHODS: CpnIq/G and IgM were determined by enzyme-linked immunosorbent assay (ELBA) in 41 well-established PBC patients and two race-matched control groups (post-hepatitis cirrhosis, n = 70; healthy controls, n = 57). RESULTS: The mean level and seroprevalence of Cpn IgG in PBC group and post-hepatitis cirrhosis (PHC) group were significantly higher than those in healthy controls (46.8±43.4 RU/mL, 49.5±45.2 RU/mL vs28.3±32.7 RU/mL; 68.3%, 71.4%, 42.1%, respectively; P<0.05). There was a remarkably elevated seroprevalence of Cpn IgM in patients with PBC (22.0%) compared to the PHC and healthy control (HC) groups. For the PBC patients versus the HCs, the odds ratios (ORs) of the presence of Cpn IgG and IgM were 2.7 (95% CI 0.9-6.1) and 5.1 (95% CI 1.4-18.5), respectively. Though there was no correlation in the level of Cpn IgG with total IgG in sera of patients with PBC (r = -0.857, P = 0.344>0.05), Cpn IgM was related with the abnormally high concentrations of total IgM in PBC group. CONCLUSION: The results of this study do not support the hypothesis that infection with Chlamydia pneumoniae may be a triggering agent or even a causative agent in PBC, but suggest that Chlamydia pneumoniae infection probably contributes to the high level of IgM present in most patients with PBC.
文摘Research data showed that syndromic approach could successfully manage gonococcal and chlamydial infections in males and syphilis and chancroid in males and females. However, low sensitivity, specificity and positive predictive value were found in the syndromic management of vaginal discharge. It is recommended that the syndromic algorithm for management of vaginal discharge used when serving high-risk and symptomatic women.
文摘Chlamydia, a human pathogen that mediates chronic, persistent and repeated infection progress, is capable of inflicting permanent scar formation. A typical reticular body is found in persistent infections. Chlamydial Hsp 60, interferon(IFN) -gamma and other cytokines function in the course of repeated infection. Immunopathological factors mediate chronic infection.
文摘To investigate the DNA sequence polymorphism of Chlamydia traehomatis ompl gene, urogenital samples were collected from 4 different cities in South China, DNA was extracted, and an approx- imately 980-bp-long fragment of the ompl gene was amplified by nested polymerase chain reaction （nPCR）. DNA sequence was determined, genotyping was performed by BLAST similarity search, and multiple alignment was performed with CLUSTAL X. Then a phylogenetie tree was constructed by Mega 3 software to illustrate the evolutionary relationships between clinical isolates and reference strains. Ninetysix specimens were sequenced, and 28 genetic variants were detected, among which E was the most prevalent genotype. The ompl gene was highly conserved for genotypes E and F, but appeared slightly less conserved for other genotypes, where the sequences displayed one to several nueleotide substitutions relative to the reference sequence. Phylogenetie tree showed that C. traehomatis serotypes were mainly divided into three clusters, according to previous grouping in the B, F-G, and C complexes, and the clinical isolates were highly related to the corresponding reference strains. It concluded that the ompl gene of the isolated C. traehomatis strains exhibited remarkable DNA sequence polymorphism, which can encourage for vaccine design and infection control.
