In this experiment, lignite was refined and processed through binderless briquette preparation process from low-rank coal and became briquette. Then, lignite and its briquette were pyrolysed as materials to compare th...In this experiment, lignite was refined and processed through binderless briquette preparation process from low-rank coal and became briquette. Then, lignite and its briquette were pyrolysed as materials to compare the nature of their pyrolysis. In this study, the experiment was carried out through a lab tube furnace, at a heating rate of 10 ~C/min, and the gas was analyzed and compared, which was collected at different temperatures. The results show that： in the pyrolysis temperature of 550-850 ℃, the semi-coke yield of briquette is 2%-6% higher than lignite, the tar yield of briquette is 2%-3% higher than lignite and the gas yield of briquette is 4%-9% less than lignite. The time required for complete release of the briquette is about 20 min less than lignite. The components in the pyrolysis gas of lignite and its briquette are the same, and their variation with the pyrolysis temperature is similar.展开更多
AIM To investigate the role of the miR-133a-UCP2 pathway in the pathogenesis of inflammatory bowel disease (IBD) and to explore the potential downstream mechanisms with respect to inflammation, oxidative stress and en...AIM To investigate the role of the miR-133a-UCP2 pathway in the pathogenesis of inflammatory bowel disease (IBD) and to explore the potential downstream mechanisms with respect to inflammation, oxidative stress and energy metabolism. METHODS C57BL/6 mice were fed dextran sulfate sodium (DSS) liquid for 7 consecutive days, followed by the administration of saline to the DSS group, UCP2 siRNA to the UCP2 group and a miR-133a mimic to the miR-133a group on days 8 and 11. Body weight, stool consistency and rectal bleeding were recorded daily, and these composed the disease activity index (DAI) score for the assessment of disease severity. After cervical dislocation was performed on day 14, the length of the colon in each mouse was measured, and colonic tissue was collected for further study, which included the following: haematoxylin and eosin staining, UCP2 and miR-133a detection by immunohistochemical staining, western blot and quantitative real-time PCR, measurement of apoptosis by TUNEL assay, and the assessment of inflammation (TNF-alpha, IL-1 beta, IL-6 and MCP1), oxidative stress (H2O2 and MDA) and metabolic parameters (ATP) by ELISA and colorimetric methods. RESULTS An animal model of IBD was successfully established, as shown by an increased DAI score, shortened colon length and specific pathologic changes, along with significantly increased UCP2 and decreased miR-133a levels. Compared with the DSS group, the severity of IBD was alleviated in the UCP2 and the miR-133a groups after successful UCP2 knockdown and miR-133a overexpression. The extent of apoptosis, as well as the levels of TNF-alpha, IL-1 beta, MDA and ATP, were significantly increased in both the UCP2 and miR-133a groups compared with the DSS group. CONCLUSION The miR-133a-UCP2 pathway participates in IBD by altering downstream inflammation, oxidative stress and markers of energy metabolism, which provides novel clues and potential therapeutic targets for IBD.展开更多
文摘In this experiment, lignite was refined and processed through binderless briquette preparation process from low-rank coal and became briquette. Then, lignite and its briquette were pyrolysed as materials to compare the nature of their pyrolysis. In this study, the experiment was carried out through a lab tube furnace, at a heating rate of 10 ~C/min, and the gas was analyzed and compared, which was collected at different temperatures. The results show that： in the pyrolysis temperature of 550-850 ℃, the semi-coke yield of briquette is 2%-6% higher than lignite, the tar yield of briquette is 2%-3% higher than lignite and the gas yield of briquette is 4%-9% less than lignite. The time required for complete release of the briquette is about 20 min less than lignite. The components in the pyrolysis gas of lignite and its briquette are the same, and their variation with the pyrolysis temperature is similar.
基金National Natural Science Foundation of China,No.81370008 and No.81000169Natural Science Foundation of Zhejiang Province,No.R2110159,No.LY15H030006 and No.LY16H030003
文摘AIM To investigate the role of the miR-133a-UCP2 pathway in the pathogenesis of inflammatory bowel disease (IBD) and to explore the potential downstream mechanisms with respect to inflammation, oxidative stress and energy metabolism. METHODS C57BL/6 mice were fed dextran sulfate sodium (DSS) liquid for 7 consecutive days, followed by the administration of saline to the DSS group, UCP2 siRNA to the UCP2 group and a miR-133a mimic to the miR-133a group on days 8 and 11. Body weight, stool consistency and rectal bleeding were recorded daily, and these composed the disease activity index (DAI) score for the assessment of disease severity. After cervical dislocation was performed on day 14, the length of the colon in each mouse was measured, and colonic tissue was collected for further study, which included the following: haematoxylin and eosin staining, UCP2 and miR-133a detection by immunohistochemical staining, western blot and quantitative real-time PCR, measurement of apoptosis by TUNEL assay, and the assessment of inflammation (TNF-alpha, IL-1 beta, IL-6 and MCP1), oxidative stress (H2O2 and MDA) and metabolic parameters (ATP) by ELISA and colorimetric methods. RESULTS An animal model of IBD was successfully established, as shown by an increased DAI score, shortened colon length and specific pathologic changes, along with significantly increased UCP2 and decreased miR-133a levels. Compared with the DSS group, the severity of IBD was alleviated in the UCP2 and the miR-133a groups after successful UCP2 knockdown and miR-133a overexpression. The extent of apoptosis, as well as the levels of TNF-alpha, IL-1 beta, MDA and ATP, were significantly increased in both the UCP2 and miR-133a groups compared with the DSS group. CONCLUSION The miR-133a-UCP2 pathway participates in IBD by altering downstream inflammation, oxidative stress and markers of energy metabolism, which provides novel clues and potential therapeutic targets for IBD.
