A novel fuzzy linear discriminant analysis method by the canonical correlation analysis （fuzzy-LDA/CCA）is presented and applied to the facial expression recognition. The fuzzy method is used to evaluate the degree o...A novel fuzzy linear discriminant analysis method by the canonical correlation analysis （fuzzy-LDA/CCA）is presented and applied to the facial expression recognition. The fuzzy method is used to evaluate the degree of the class membership to which each training sample belongs. CCA is then used to establish the relationship between each facial image and the corresponding class membership vector, and the class membership vector of a test image is estimated using this relationship. Moreover, the fuzzy-LDA/CCA method is also generalized to deal with nonlinear discriminant analysis problems via kernel method. The performance of the proposed method is demonstrated using real data.展开更多
An analytical model of the surface field distribution and breakdown voltage of the reduced surface field lateral double diffusion MOS transistor is proposed.Based on the 2-D Poisson's equation solution,the derived...An analytical model of the surface field distribution and breakdown voltage of the reduced surface field lateral double diffusion MOS transistor is proposed.Based on the 2-D Poisson's equation solution,the derived model gives the closed form solutions of the surface potential and electrical field distributions as a function of the structure parameters and drain bias.A criterion for obtaining the optimal trade-off between the breakdown voltage and on-resistance is also presented to serve to quantify the maximum breakdown voltage and optimal relations of all design parameters.Analytical results are shown in good agreement with the numerical analysis obtained by the semiconductor device simulator MEDICI and previous reported experimental data.展开更多
To analyze the stress state of steel orthotropic deck pavement and provide reference for the design of the overlay, the inner stress state and strain distribution of surfacing under the load of the deformation of the ...To analyze the stress state of steel orthotropic deck pavement and provide reference for the design of the overlay, the inner stress state and strain distribution of surfacing under the load of the deformation of the whole bridge structure and tyre load are analyzed by the finite element method of submodeling. Influence of surfacing modulus on the strain state of the overlay is analyzed for the purpose of the optimal design of the overlay structure. Analysis results show that the deformation of the whole bridge structure has no evident influence on the stress state of the overlay. The key factor of the overlay design is the transverse tensile strain in the overlay above the upper edge of web plate of rib. The stress state of the overlay is influenced evidently by the modulus of rigidity transform overlay. And the stress state of the overlay can be optimized and lowered by increasing the modulus and thickness of rigidity transform overlay, The fatigue test has been done to evaluate the fatigue performance and modulus of different deck pavement materials such as epoxy asphalt, SBS modified asphalt, rosphalt asphalt which can provide reference for deck pavement structure design.展开更多
The dynamic ground subsidence due to underground mining is a complicated time-dependent and rate- dependent process. Based. on the theory of rock rheology and probability integral method, this study developed the supe...The dynamic ground subsidence due to underground mining is a complicated time-dependent and rate- dependent process. Based. on the theory of rock rheology and probability integral method, this study developed the superposltlOn model for the prediction and analysis of the ground dynamic subsidence in mining area of thick !oose layer. The model consists of two parts （the prediction of overlying bedrock and the prediction of thick loose layer）. The overlying bedrock is regarded as visco-elastic beam, of which the dynamic subsidence is predicted by the Kelvin visco-elastic rheological model. The thick loose layer is regarded as random medium, and the ground dynamic subsidence, is predicted by the probability integral model. At last, the two prediction models are vertically stacked in the same coordinate system, and the bedrock dynamic subsidence is regarded as a variable mining thickness input into the prediction model of ground dynamic subsidence. The prediction results obtained were compared w^th actual movement and deformation data from Zhao I and Zhao II mine, central China. The agreement of the prediction results with the. field measurements.show that the superposition model （SM） is more satisfactory and the formulae obtained are more effective than the classical single probability Integral model（SPIM）, and thus can be effectively used for predicting the ground dynamic subsidence in mining area of thick loose layer.展开更多
The veracity of land evaluation is tightly related to the reasonable weights of land evaluation fac- tors. By mapping qualitative linguistic words into a fine-changeable cloud drops and translating the uncertain facto...The veracity of land evaluation is tightly related to the reasonable weights of land evaluation fac- tors. By mapping qualitative linguistic words into a fine-changeable cloud drops and translating the uncertain factor conditions into quantitative values with the uncertain illation based on cloud model, and then, inte- grating correlation analysis, a new way of figuring out the weight of land evaluation factors is proposed. It may solve the limitations of the conventional ways.展开更多
Adsorption process is acknowledged as an effective option for phenolic wastewater treatment. In this work, the activated carbon （AC） samples after thermal modification were prepared by using muffle furnace. The phen...Adsorption process is acknowledged as an effective option for phenolic wastewater treatment. In this work, the activated carbon （AC） samples after thermal modification were prepared by using muffle furnace. The phenol ad- sorption kinetics and equilibrium measurements were carried out under static conditions at temperature ranging from 25 to 55 ~C. The test results show that the thermal modification can enhance phenol adsorption on AC samples. The porous structure and surface chemistry analyses indicate that the decay in pore morphology and decrease of total oxygen-containing functional groups are found for the thermal modified AC samples. Thus, it can be further inferred that the decrease of total oxygen-containing functional groups on the modified AC sam- ples is the main reason for the enhanced phenol adsorption capacity. For both the raw sample and the optimum modified AC sample at 900 ~C, the pseudo-second order kinetics and Langmuir models are found to fit the exper- imental data very well. The maximum phenol adsorption capacity of the optimum modified AC sample can reach 144,93 mg.g ~ which is higher than that of the raw sample, i.e. 119.53 mg.g 1. Adsorption thermodynamics analysis confirms that the phenol adsorption on the optimum modified AC sample is an exothermic process and mainly via physical adsorption.展开更多
Integrated with GIS and remote sensing(RS) technology,a systematic analysis and its methodology for human-settlements social environment has been introduced.This methodology has been called spatial trend field model(S...Integrated with GIS and remote sensing(RS) technology,a systematic analysis and its methodology for human-settlements social environment has been introduced.This methodology has been called spatial trend field model(STFM).STFM's application history in the field of human-settlements social environment has been discussed at first.Then,some index data models have been created through STFM,which include population density trend field,human activity strength trend field,city-town spatial density trend field,urbanization ratio trend field,road density trend field,GDP spatial density trend field and PER-GDP spatial density trend field.With all above-mentioned indexes as input data,through Iterative Self-Organizing Data Analysis Techniques Algorithm(ISODATA),this paper makes a verification study of Chongqing municipality.The result of the case study confirms that STFM methodology is credible and has high efficiency for regional human-settlements study.展开更多
In analyzing seismic stability of a slope with upper bound limit analysis method, the slip surface is often assumed as a log-spiral or plane slip surface. However, due to the presence of a weak layer and unfavorable g...In analyzing seismic stability of a slope with upper bound limit analysis method, the slip surface is often assumed as a log-spiral or plane slip surface. However, due to the presence of a weak layer and unfavorable geological structural surface or a bedrock interface with overlying soft strata, the preexisting slip surface of the slope may be irregular and composed of a series of planes rather than strictly logspiral or plane shape. A computational model is developed for analyzing the seismic stability of slopes with pre-existing slip surfaces. This model is based on the upper bound limit analysis method and can consider the effect of anchor bolts. The soil or rock is deemed to follow the Mohr-Coulomb yield criterion. The slope is divided into multiple block elements along the slip surface. According to the displacement compatibility and the associated flow rule, a kinematic velocity field of the slope can be obtained computationally. The proposed model allows not only calculation of the rate of external work owing to the combined effect of self-weight and seismic loading, but also that of the energy dissipation rate caused by the slip surface, interfaces of block elements and anchorage effect of the anchors. Considering a direct relationship between the rate of external work and the energy dissipation rate, the expressions of yield acceleration and permanent displacement of anchored slopes can be derived. Finally, the validity of this proposed model is illustrated by analysis on three typical slopes. The results showed that the proposed model is more easily formulated and does not need to solve complex equations or time consuming iterations compared with previous methods based on the conditions of force equilibrium.展开更多
Based on the image theory,the analytical solutions of tunneling-induced ground displacement were derived in conjunction with the nonuniform convergence model.The reasonable value of Poisson ratio in the analytical sol...Based on the image theory,the analytical solutions of tunneling-induced ground displacement were derived in conjunction with the nonuniform convergence model.The reasonable value of Poisson ratio in the analytical solution was discussed.The ground settlement width parameter which could reflect the ground condition was introduced to modify the analytical solutions proposed above,and new analytical solutions were presented.To evaluate the validity of the present solutions using the nonuniform convergence model,the results were compared with the observed values for four engineering projects,including 38 measured data of ground settlement.The agreement shows that the present solutions using the nonuniform convergence model are effective for evaluating the tunneling-induced ground displacements.展开更多
AIM:To investigate the variability of the main immunodominant motifs of hepatitis B virus(HBV) core gene by ultra-deep-pyrosequencing(UDPS).METHODS:Four samples(2 genotype A and 2 genotype D) from 4 treatment-na ve pa...AIM:To investigate the variability of the main immunodominant motifs of hepatitis B virus(HBV) core gene by ultra-deep-pyrosequencing(UDPS).METHODS:Four samples(2 genotype A and 2 genotype D) from 4 treatment-na ve patients were assessed for baseline variability.Two additional samples from one patient(patient 4,genotype D) were selected for analysis:one sample corresponded to a 36-mo treatment-free period from baseline and the other to the time of viral breakthrough after 18 mo of lamivudine treatment.The HBV region analyzed covered amino acids 40 to 95 of the core gene,and included the two main epitopic regions,Th50-69 and B74-84.UDPS was carried out in the Genome Sequencer FLX system(454 Life Sciences,Roche).After computer filtering of UDPS data based on a Poisson statistical model,122 813 sequences were analyzed.The most conserved position detected by UDPS was analyzed by site-directed mutagenesis and evaluated in cell culture.RESULTS:Positions with highest variability rates were mainly located in the main core epitopes,confirming their role as immune-stimulating regions.In addition,the distribution of variability showed a relationship with HBV genotype.Patient 1(genotype A) presented the lowest variability rates and patient 2(genotype A) had 3 codons with variability higher than 1%.Patient 3 and 4(both genotype D) presented 5 and 8 codons with variability higher than 1%,respectively.The median baseline frequencies showed that genotype A samples had higher variability in epitopic positions than in the other positions analyzed,approaching significance(P = 0.07,sample 1 and P = 0.05,sample 2).In contrast,there were no significant differences in variability between the epitopic and other positions in genotype D cases.Interestingly,patient 1 presented a completely mutated motif from amino acid 64 to 67(E 64 LMT 67),which is commonly recognized by T helper cells.Additionally,the variability observed in all 4 patients was particularly associated with the E 64 LMT 67 motif.Codons 78 and 79 were highly conserved in all samples,in keeping with their involvement in the interaction between the HBV virion capsid and the surface antigens(HBsAg).Of note,codon 76 was even more conserved than codons 78 and 79,suggesting a possible role in HBsAg interactions or even in hepatitis B e antigen conformation.Sequential analysis of samples from patient 4(genotype D) illustrated the dynamism of the HBV quasispecies,with strong selection of one minor baseline variant coinciding with a decrease in core variability during the treatment-free and lamivudinetreated period.The drop in variability seemed to result from a "steady state" situation of the HBV quasispecies after selection of the variant with greatest fitness.CONCLUSION:Host immune pressure seems to be the main cause of HBV core evolution.UDPS analysis is a useful technique for studying viral quasispecies.展开更多
Electromechanical dynamics analysis and simulation on a rollforming equipment with both sides variable cross-section are discussed in this study.The system includes mechanical parts and electromagnetism parts,and it i...Electromechanical dynamics analysis and simulation on a rollforming equipment with both sides variable cross-section are discussed in this study.The system includes mechanical parts and electromagnetism parts,and it is a strongly coupled electromechanical system.Based on a virtual work principle and given power,generalized forces of this system are obtained.By using Lagrange-Maxwell equations,a model of electromechanical dynamics is established.Differential equations of two-phase winding on d-q axis are obtained by Park transformation,which comes from three-phase winding equations on the A-B-C axis.This system is solved with the 4th order Runge-Kutta's method,and discrete solutions of all variables are obtained.Finally,by using Matlab language,the system is simulated.The results show that the proposed method works very well.展开更多
The transmission shaft of the underground screw drill fractured when milling-shoe and grinding bridge plug was applied to the coiled tubes in a horizontal well of Sichuan province, but the position of this transmissio...The transmission shaft of the underground screw drill fractured when milling-shoe and grinding bridge plug was applied to the coiled tubes in a horizontal well of Sichuan province, but the position of this transmission shaft fracture did not occur at the minor-diameter retracting position and reducing position that were easy to fracture. An analysis of the transverse planes of the fracture found that the cause of the transmission shaft fracture surface was that the defects of the initial surface were propagating to cracks and gave rise to the fracture under torque load. To specifically know well the strength of the transmission shaft under damaging fracture, a statics analysis was conducted on the transmission shaft through ANSYS finite element simulation software, and the finite element models under no cracks, different-depth circumferential cracks, and similar situation of the transverse planes of fracture were established respectively. An analysis of the crack-free finite element model found that the fracture of the transmission shaft was really not caused by the self-structure of the transmission shaft; an analysis of circumferential crack finite element model found that strong stress concentration would appear in the tip of cracks, and the value of the stress would increase along with the increase of the circumferential crack depth, the stress of the entire crack top tended to fluctuate like waves, and also the strength of the transmission shaft was greatly impacted by the presence of cracks; an analysis of the similar crack finite element model found that stress concentration would appear in the tip of cracks, and the initial cracks always started to propagate from the tip of the external surface of the transmission shaft and would propagate inward until the propagating areas of two cracks overlapped, and finally reached the position of transient interruption, and then the transmission shaft fractured completely and the fracture strength was onlv 1/5 of that under no cracks.展开更多
A model for liquid-gas flow （MLGF）, considering the flee movement of liquid surface, was built to simulate the wastewater velocity field and gas distribution in a full-scale Caroussel oxidation ditch with surface ae...A model for liquid-gas flow （MLGF）, considering the flee movement of liquid surface, was built to simulate the wastewater velocity field and gas distribution in a full-scale Caroussel oxidation ditch with surface aeration. It was calibrated and validated by field measurement data, and the calibrated parameters and sections were selected based on both model analysis and numerical computation. The simulated velocities of MLGF were compared to that of a model for wastewater-sludge flow （MWSF）. The results show that the free liquid surface considered in MLGF improves the simulated velocity results of upper layer and surface. Moreover, distribution of gas volume fraction （GVF） simulated by MLGF was compared to dissolved oxygen （DO） measured in the oxidation ditch. It is shown that DO distribution is affected by many factors besides GVF distribution.展开更多
The cutter runout effect has significant influence on the shape of cutter swept surface and the machining surface quality. Hence,it is necessary to integrate the cutter runout effect in cutter swept surface modeling,g...The cutter runout effect has significant influence on the shape of cutter swept surface and the machining surface quality. Hence,it is necessary to integrate the cutter runout effect in cutter swept surface modeling,geometric error prediction and tool path optimization for five-axis flank machining. In this paper,an envelope surface model considering cutter runout effect is first established,and geometric errors induced by runout effect are derived based on the relative motion analysis between the cutter and part in machining. In the model,the cutter runout is defined by four parameters,including inclination angle,location angle,offset value and the length of cutter axis. Then the runout parameters are integrated into the rotation surface of each cutting edge that is used to form the final cutter envelope surface for the five-axis machining process. Thus,the final resulting geometric errors of the machined surface induced by cutter runout can be obtained through computing the deviations from the nominal cutter swept surface. To reduce these errors,an iterative least square method is used to optimize the tool paths for five-axis flank machining. Finally,a validation example is given for a specific ruled surface. Results show the effectiveness and feasibility of the analytical model of geometric errors induced by cutter runout,and also show that the geometric errors can be reduced significantly using the proposed tool path planning method.展开更多
基金The National Natural Science Foundation of China (No.60503023,60872160)the Natural Science Foundation for Universities ofJiangsu Province (No.08KJD520009)the Intramural Research Foundationof Nanjing University of Information Science and Technology(No.Y603)
文摘A novel fuzzy linear discriminant analysis method by the canonical correlation analysis （fuzzy-LDA/CCA）is presented and applied to the facial expression recognition. The fuzzy method is used to evaluate the degree of the class membership to which each training sample belongs. CCA is then used to establish the relationship between each facial image and the corresponding class membership vector, and the class membership vector of a test image is estimated using this relationship. Moreover, the fuzzy-LDA/CCA method is also generalized to deal with nonlinear discriminant analysis problems via kernel method. The performance of the proposed method is demonstrated using real data.
基金Supported by National Natural Science Foundation of China(NSFC)(51605465)Research Project of Scientific Research Equipment of The Chinese Academy of Sciences~~
文摘An analytical model of the surface field distribution and breakdown voltage of the reduced surface field lateral double diffusion MOS transistor is proposed.Based on the 2-D Poisson's equation solution,the derived model gives the closed form solutions of the surface potential and electrical field distributions as a function of the structure parameters and drain bias.A criterion for obtaining the optimal trade-off between the breakdown voltage and on-resistance is also presented to serve to quantify the maximum breakdown voltage and optimal relations of all design parameters.Analytical results are shown in good agreement with the numerical analysis obtained by the semiconductor device simulator MEDICI and previous reported experimental data.
文摘To analyze the stress state of steel orthotropic deck pavement and provide reference for the design of the overlay, the inner stress state and strain distribution of surfacing under the load of the deformation of the whole bridge structure and tyre load are analyzed by the finite element method of submodeling. Influence of surfacing modulus on the strain state of the overlay is analyzed for the purpose of the optimal design of the overlay structure. Analysis results show that the deformation of the whole bridge structure has no evident influence on the stress state of the overlay. The key factor of the overlay design is the transverse tensile strain in the overlay above the upper edge of web plate of rib. The stress state of the overlay is influenced evidently by the modulus of rigidity transform overlay. And the stress state of the overlay can be optimized and lowered by increasing the modulus and thickness of rigidity transform overlay, The fatigue test has been done to evaluate the fatigue performance and modulus of different deck pavement materials such as epoxy asphalt, SBS modified asphalt, rosphalt asphalt which can provide reference for deck pavement structure design.
基金provided by the National Natural Science Foundation of China Youth Found of China (No.41102169)the doctoral foundation of Henan Polytechnic University of China (No. B2014-056)
文摘The dynamic ground subsidence due to underground mining is a complicated time-dependent and rate- dependent process. Based. on the theory of rock rheology and probability integral method, this study developed the superposltlOn model for the prediction and analysis of the ground dynamic subsidence in mining area of thick !oose layer. The model consists of two parts （the prediction of overlying bedrock and the prediction of thick loose layer）. The overlying bedrock is regarded as visco-elastic beam, of which the dynamic subsidence is predicted by the Kelvin visco-elastic rheological model. The thick loose layer is regarded as random medium, and the ground dynamic subsidence, is predicted by the probability integral model. At last, the two prediction models are vertically stacked in the same coordinate system, and the bedrock dynamic subsidence is regarded as a variable mining thickness input into the prediction model of ground dynamic subsidence. The prediction results obtained were compared w^th actual movement and deformation data from Zhao I and Zhao II mine, central China. The agreement of the prediction results with the. field measurements.show that the superposition model （SM） is more satisfactory and the formulae obtained are more effective than the classical single probability Integral model（SPIM）, and thus can be effectively used for predicting the ground dynamic subsidence in mining area of thick loose layer.
文摘The veracity of land evaluation is tightly related to the reasonable weights of land evaluation fac- tors. By mapping qualitative linguistic words into a fine-changeable cloud drops and translating the uncertain factor conditions into quantitative values with the uncertain illation based on cloud model, and then, inte- grating correlation analysis, a new way of figuring out the weight of land evaluation factors is proposed. It may solve the limitations of the conventional ways.
基金Supported by the National Natural Science Foundation of China(41302132)Training Programmes of Innovation and Entrepreneurship for Undergraduates of Yunnan Province(Grant No.201510674042)the Introduced Talents Foundation of Kunming University of Science and Technology(KKSY201205160)
文摘Adsorption process is acknowledged as an effective option for phenolic wastewater treatment. In this work, the activated carbon （AC） samples after thermal modification were prepared by using muffle furnace. The phenol ad- sorption kinetics and equilibrium measurements were carried out under static conditions at temperature ranging from 25 to 55 ~C. The test results show that the thermal modification can enhance phenol adsorption on AC samples. The porous structure and surface chemistry analyses indicate that the decay in pore morphology and decrease of total oxygen-containing functional groups are found for the thermal modified AC samples. Thus, it can be further inferred that the decrease of total oxygen-containing functional groups on the modified AC sam- ples is the main reason for the enhanced phenol adsorption capacity. For both the raw sample and the optimum modified AC sample at 900 ~C, the pseudo-second order kinetics and Langmuir models are found to fit the exper- imental data very well. The maximum phenol adsorption capacity of the optimum modified AC sample can reach 144,93 mg.g ~ which is higher than that of the raw sample, i.e. 119.53 mg.g 1. Adsorption thermodynamics analysis confirms that the phenol adsorption on the optimum modified AC sample is an exothermic process and mainly via physical adsorption.
基金supported by National 11th Five-Year Technology Support Program (Grant No 2008BAH31B06)National Natural Science Foundation of China (Grant No50738007)
文摘Integrated with GIS and remote sensing(RS) technology,a systematic analysis and its methodology for human-settlements social environment has been introduced.This methodology has been called spatial trend field model(STFM).STFM's application history in the field of human-settlements social environment has been discussed at first.Then,some index data models have been created through STFM,which include population density trend field,human activity strength trend field,city-town spatial density trend field,urbanization ratio trend field,road density trend field,GDP spatial density trend field and PER-GDP spatial density trend field.With all above-mentioned indexes as input data,through Iterative Self-Organizing Data Analysis Techniques Algorithm(ISODATA),this paper makes a verification study of Chongqing municipality.The result of the case study confirms that STFM methodology is credible and has high efficiency for regional human-settlements study.
基金financially supported by the NSFC-ICIMOD joint project(41761144077)the Light of West“Belt&Road”international cooperation team project of Chinese Academy of Sciences(Su Lijun)+1 种基金the Hundred Talents Program of Chinese Academy of Sciences(Su Lijun)the NSFC(National Natural Science Foundation of China)project(51278397)
文摘In analyzing seismic stability of a slope with upper bound limit analysis method, the slip surface is often assumed as a log-spiral or plane slip surface. However, due to the presence of a weak layer and unfavorable geological structural surface or a bedrock interface with overlying soft strata, the preexisting slip surface of the slope may be irregular and composed of a series of planes rather than strictly logspiral or plane shape. A computational model is developed for analyzing the seismic stability of slopes with pre-existing slip surfaces. This model is based on the upper bound limit analysis method and can consider the effect of anchor bolts. The soil or rock is deemed to follow the Mohr-Coulomb yield criterion. The slope is divided into multiple block elements along the slip surface. According to the displacement compatibility and the associated flow rule, a kinematic velocity field of the slope can be obtained computationally. The proposed model allows not only calculation of the rate of external work owing to the combined effect of self-weight and seismic loading, but also that of the energy dissipation rate caused by the slip surface, interfaces of block elements and anchorage effect of the anchors. Considering a direct relationship between the rate of external work and the energy dissipation rate, the expressions of yield acceleration and permanent displacement of anchored slopes can be derived. Finally, the validity of this proposed model is illustrated by analysis on three typical slopes. The results showed that the proposed model is more easily formulated and does not need to solve complex equations or time consuming iterations compared with previous methods based on the conditions of force equilibrium.
基金Project(09JJ1008) supported by Hunan Provincial Science Foundation of China
文摘Based on the image theory,the analytical solutions of tunneling-induced ground displacement were derived in conjunction with the nonuniform convergence model.The reasonable value of Poisson ratio in the analytical solution was discussed.The ground settlement width parameter which could reflect the ground condition was introduced to modify the analytical solutions proposed above,and new analytical solutions were presented.To evaluate the validity of the present solutions using the nonuniform convergence model,the results were compared with the observed values for four engineering projects,including 38 measured data of ground settlement.The agreement shows that the present solutions using the nonuniform convergence model are effective for evaluating the tunneling-induced ground displacements.
基金Supported by A Grant from the Spanish Ministry of Health and Consumer Affairs,No. FIS PS09/00899 and SAF 2009-10403CIBERehd Funded by Instituto CarlosⅢ,Ministry of Health and Consumer Affairs
文摘AIM:To investigate the variability of the main immunodominant motifs of hepatitis B virus(HBV) core gene by ultra-deep-pyrosequencing(UDPS).METHODS:Four samples(2 genotype A and 2 genotype D) from 4 treatment-na ve patients were assessed for baseline variability.Two additional samples from one patient(patient 4,genotype D) were selected for analysis:one sample corresponded to a 36-mo treatment-free period from baseline and the other to the time of viral breakthrough after 18 mo of lamivudine treatment.The HBV region analyzed covered amino acids 40 to 95 of the core gene,and included the two main epitopic regions,Th50-69 and B74-84.UDPS was carried out in the Genome Sequencer FLX system(454 Life Sciences,Roche).After computer filtering of UDPS data based on a Poisson statistical model,122 813 sequences were analyzed.The most conserved position detected by UDPS was analyzed by site-directed mutagenesis and evaluated in cell culture.RESULTS:Positions with highest variability rates were mainly located in the main core epitopes,confirming their role as immune-stimulating regions.In addition,the distribution of variability showed a relationship with HBV genotype.Patient 1(genotype A) presented the lowest variability rates and patient 2(genotype A) had 3 codons with variability higher than 1%.Patient 3 and 4(both genotype D) presented 5 and 8 codons with variability higher than 1%,respectively.The median baseline frequencies showed that genotype A samples had higher variability in epitopic positions than in the other positions analyzed,approaching significance(P = 0.07,sample 1 and P = 0.05,sample 2).In contrast,there were no significant differences in variability between the epitopic and other positions in genotype D cases.Interestingly,patient 1 presented a completely mutated motif from amino acid 64 to 67(E 64 LMT 67),which is commonly recognized by T helper cells.Additionally,the variability observed in all 4 patients was particularly associated with the E 64 LMT 67 motif.Codons 78 and 79 were highly conserved in all samples,in keeping with their involvement in the interaction between the HBV virion capsid and the surface antigens(HBsAg).Of note,codon 76 was even more conserved than codons 78 and 79,suggesting a possible role in HBsAg interactions or even in hepatitis B e antigen conformation.Sequential analysis of samples from patient 4(genotype D) illustrated the dynamism of the HBV quasispecies,with strong selection of one minor baseline variant coinciding with a decrease in core variability during the treatment-free and lamivudinetreated period.The drop in variability seemed to result from a "steady state" situation of the HBV quasispecies after selection of the variant with greatest fitness.CONCLUSION:Host immune pressure seems to be the main cause of HBV core evolution.UDPS analysis is a useful technique for studying viral quasispecies.
基金Supported by the National Science and Technology Support Program(No.2011BAG03B03)Inner Mongolia University of Technology Science and Research Projects of China(No.X201338)
文摘Electromechanical dynamics analysis and simulation on a rollforming equipment with both sides variable cross-section are discussed in this study.The system includes mechanical parts and electromagnetism parts,and it is a strongly coupled electromechanical system.Based on a virtual work principle and given power,generalized forces of this system are obtained.By using Lagrange-Maxwell equations,a model of electromechanical dynamics is established.Differential equations of two-phase winding on d-q axis are obtained by Park transformation,which comes from three-phase winding equations on the A-B-C axis.This system is solved with the 4th order Runge-Kutta's method,and discrete solutions of all variables are obtained.Finally,by using Matlab language,the system is simulated.The results show that the proposed method works very well.
文摘The transmission shaft of the underground screw drill fractured when milling-shoe and grinding bridge plug was applied to the coiled tubes in a horizontal well of Sichuan province, but the position of this transmission shaft fracture did not occur at the minor-diameter retracting position and reducing position that were easy to fracture. An analysis of the transverse planes of the fracture found that the cause of the transmission shaft fracture surface was that the defects of the initial surface were propagating to cracks and gave rise to the fracture under torque load. To specifically know well the strength of the transmission shaft under damaging fracture, a statics analysis was conducted on the transmission shaft through ANSYS finite element simulation software, and the finite element models under no cracks, different-depth circumferential cracks, and similar situation of the transverse planes of fracture were established respectively. An analysis of the crack-free finite element model found that the fracture of the transmission shaft was really not caused by the self-structure of the transmission shaft; an analysis of circumferential crack finite element model found that strong stress concentration would appear in the tip of cracks, and the value of the stress would increase along with the increase of the circumferential crack depth, the stress of the entire crack top tended to fluctuate like waves, and also the strength of the transmission shaft was greatly impacted by the presence of cracks; an analysis of the similar crack finite element model found that stress concentration would appear in the tip of cracks, and the initial cracks always started to propagate from the tip of the external surface of the transmission shaft and would propagate inward until the propagating areas of two cracks overlapped, and finally reached the position of transient interruption, and then the transmission shaft fractured completely and the fracture strength was onlv 1/5 of that under no cracks.
基金Project supported by Visiting Scholar Foundation of Key Laboratory of the Resources Exploitation and Environmental Disaster Control Engineering in Southwest China (Chongqing University),Ministry of Education,China
文摘A model for liquid-gas flow （MLGF）, considering the flee movement of liquid surface, was built to simulate the wastewater velocity field and gas distribution in a full-scale Caroussel oxidation ditch with surface aeration. It was calibrated and validated by field measurement data, and the calibrated parameters and sections were selected based on both model analysis and numerical computation. The simulated velocities of MLGF were compared to that of a model for wastewater-sludge flow （MWSF）. The results show that the free liquid surface considered in MLGF improves the simulated velocity results of upper layer and surface. Moreover, distribution of gas volume fraction （GVF） simulated by MLGF was compared to dissolved oxygen （DO） measured in the oxidation ditch. It is shown that DO distribution is affected by many factors besides GVF distribution.
基金supported by the National Natural Science Foundation of China (Grant No. 51075054)the National Basic Research Program of China ("973" Program) (Grant Nos. 2005CB726100 and 2011CB706800)the Fundamental Research Funds for the Central Universities (Grant No. DUT10ZD205)
文摘The cutter runout effect has significant influence on the shape of cutter swept surface and the machining surface quality. Hence,it is necessary to integrate the cutter runout effect in cutter swept surface modeling,geometric error prediction and tool path optimization for five-axis flank machining. In this paper,an envelope surface model considering cutter runout effect is first established,and geometric errors induced by runout effect are derived based on the relative motion analysis between the cutter and part in machining. In the model,the cutter runout is defined by four parameters,including inclination angle,location angle,offset value and the length of cutter axis. Then the runout parameters are integrated into the rotation surface of each cutting edge that is used to form the final cutter envelope surface for the five-axis machining process. Thus,the final resulting geometric errors of the machined surface induced by cutter runout can be obtained through computing the deviations from the nominal cutter swept surface. To reduce these errors,an iterative least square method is used to optimize the tool paths for five-axis flank machining. Finally,a validation example is given for a specific ruled surface. Results show the effectiveness and feasibility of the analytical model of geometric errors induced by cutter runout,and also show that the geometric errors can be reduced significantly using the proposed tool path planning method.
