花青素在黑果腺肋花楸中含量丰富,其具有抗氧化、抗炎抑菌、防治糖尿病、心血管保护以及抗癌、保肝等多种生物活性。本文通过查阅近年来国内外黑果腺肋花楸花青素的研究进展,对花青素提取工艺及生物活性等进行了综述。期望可以为我国在...花青素在黑果腺肋花楸中含量丰富,其具有抗氧化、抗炎抑菌、防治糖尿病、心血管保护以及抗癌、保肝等多种生物活性。本文通过查阅近年来国内外黑果腺肋花楸花青素的研究进展,对花青素提取工艺及生物活性等进行了综述。期望可以为我国在黑果腺肋花楸相关领域的研究进程和开发利用提供参考依据,从而深入挖掘黑果腺肋花楸的潜在价值与应用前景。Anthocyanins are abundant in Aronia melanocarpa, and they have multiple biological activities, including antioxidant, anti-inflammatory and antibacterial capabilities, as well as being effective in the prevention and treatment of diabetes, providing cardiovascular protection, possessing anti-cancer properties, and having a liver-protective function. Based on the research results of anthocyanins in Aronia melanocarpa at home and abroad in recent years, this paper reviews the extraction process and biological activities of anthocyanins. It is expected that it can provide a reference for the research process and development and utilization in the related fields of Aronia melanocarpa in China, so as to deeply explore the potential value and application prospects of Aronia melanocarpa.展开更多
建立一种基于美国官方分析化学师协会(Association of Official Analytical Chemists,AOAC)方法检测黑果枸杞及其制品中花青素含量的改进pH示差法。考察了黑果枸杞及其制品中花青素的最佳提取和检测条件,通过液相色谱-三重四级杆串联质...建立一种基于美国官方分析化学师协会(Association of Official Analytical Chemists,AOAC)方法检测黑果枸杞及其制品中花青素含量的改进pH示差法。考察了黑果枸杞及其制品中花青素的最佳提取和检测条件,通过液相色谱-三重四级杆串联质谱法鉴别出黑果枸杞中花青素的具体化学结构,并计算出混合花青素的平均摩尔质量。通过分光光度法测得混合花青素的平均摩尔消光系数,对改进后的pH示差法进行方法学验证和花青素的含量测定。结果显示,最佳提取和检测条件如下:黑果枸杞花青素提取溶剂为盐酸-80%(体积分数)乙醇(3∶97,体积比),料液比为1∶100(g∶mL),提取温度为50℃,提取时间为30 min,缓冲溶液稀释5倍后静置平衡20 min。液相色谱-三重四级杆串联质谱法鉴别黑果枸杞中主要以矮牵牛素类花青素为主(占97.96%),黑果枸杞特有的混合花青素平均摩尔质量为912.7 g/mol,平均摩尔消光系数为29591 L/(mol·cm)。pH示差法改进后能够满足方法学验证要求,固体样品和液体样品最低检出限分别为28.2 mg/100 g、0.282 mg/100 mL。方法改进后花青素提取增长率均大于20%,静置平衡20 min后单次检测结果精密度小于0.3%。以矮牵牛素类花青素代替矢车菊素-3-O-葡萄糖苷计算花青素含量平均提高了2.41倍,能真实地反映黑果枸杞及其制品中花青素的含量。展开更多
文摘花青素在黑果腺肋花楸中含量丰富,其具有抗氧化、抗炎抑菌、防治糖尿病、心血管保护以及抗癌、保肝等多种生物活性。本文通过查阅近年来国内外黑果腺肋花楸花青素的研究进展,对花青素提取工艺及生物活性等进行了综述。期望可以为我国在黑果腺肋花楸相关领域的研究进程和开发利用提供参考依据,从而深入挖掘黑果腺肋花楸的潜在价值与应用前景。Anthocyanins are abundant in Aronia melanocarpa, and they have multiple biological activities, including antioxidant, anti-inflammatory and antibacterial capabilities, as well as being effective in the prevention and treatment of diabetes, providing cardiovascular protection, possessing anti-cancer properties, and having a liver-protective function. Based on the research results of anthocyanins in Aronia melanocarpa at home and abroad in recent years, this paper reviews the extraction process and biological activities of anthocyanins. It is expected that it can provide a reference for the research process and development and utilization in the related fields of Aronia melanocarpa in China, so as to deeply explore the potential value and application prospects of Aronia melanocarpa.
