The murine microglial cell line BV2 has neuroprotective effects, but is toxic to neurons by secret-ing inlfammatory cytokines, and is an important target in the treatment of nerve inlfammation and neurodegenerative di...The murine microglial cell line BV2 has neuroprotective effects, but is toxic to neurons by secret-ing inlfammatory cytokines, and is an important target in the treatment of nerve inlfammation and neurodegenerative diseases. In the present study, we observed the effects of transfecting three amyloid precursor-like protein 2 （APLP2） C-terminal fragments （CTFs; C57, C50 and C31） in the pEGFP-N1 vector on S100A9 expression in BV2 cells. Reverse transcription-PCR, western blot assay and immunocytochemistry revealed that S100A9 protein and mRNA expression was greater in BV2 cells after CTF transfection than after mock transfection with an empty vector. Furthermore, transfection of full-length APLP2-751 resulted in low levels of S100A9 protein ex-pression. Our results show that APLP2-CTFs upregulate S100A9 protein and mRNA expression in BV2 cells, and identify a novel pathway involved in neuronal injury and apoptosis, and repair and protection in Alzheimer’s disease.展开更多
Dear Editor,Yellow fever（YF）,a mosquito-borne flavivirus disease,is endemic in tropical areas of Africa and Central and South America.YF is transmitted via the bite of infected Aedes aegypti or Haemogogus mosquitoes...Dear Editor,Yellow fever（YF）,a mosquito-borne flavivirus disease,is endemic in tropical areas of Africa and Central and South America.YF is transmitted via the bite of infected Aedes aegypti or Haemogogus mosquitoes and mainly affects humans and nonhuman primates.展开更多
Biological mass spectrometry has received a great progress in recent years.The osteopontin(OPN) isolated and purified from the human milk was studied by HPLC using SCX and C4 columns.After hydrolyzing the purified OPN...Biological mass spectrometry has received a great progress in recent years.The osteopontin(OPN) isolated and purified from the human milk was studied by HPLC using SCX and C4 columns.After hydrolyzing the purified OPN sample by trypsin,the correlative polypeptide fragments GDSVVYGLR and QNLLAPQTLPSK were obtained by FT-ICR-MS.It showed that the method of nano-spray HPLC combined with FT-ICR-MS and Mascot search was very efficient for the analysis of the purified OPN and its polypeptide fragments.Further study provides more academic theories of their different modifications and the relative bioactivities.展开更多
目的:探讨组织多肽特异性抗原(tissue polypeptide specific antigen,TPS)、细胞角蛋白19(cytokeratin19 fragment,CYFRA21-1)和鳞状细胞癌抗原(SCC-Ag)在肺癌患者血清中的表达水平及其对肺癌诊断的临床应用价值。方法:采用酶联免疫吸附...目的:探讨组织多肽特异性抗原(tissue polypeptide specific antigen,TPS)、细胞角蛋白19(cytokeratin19 fragment,CYFRA21-1)和鳞状细胞癌抗原(SCC-Ag)在肺癌患者血清中的表达水平及其对肺癌诊断的临床应用价值。方法:采用酶联免疫吸附法(ELISA)和免疫放射分析法(IRMA)对130例肺癌患者、67例肺部良性疾病患者及49例正常人的血清TPS,CYFRA21-1和SCC-Ag浓度进行检测。结果:肺癌组血清TPS和CYFRA21-1水平均明显高于肺部良性疾病组(P<0.01)和正常对照组(P<0.01)。TPS,CYFRA21-1和SCC-Ag阳性率与浓度均随着临床分期的进展而升高。TPS和CYFRA21-1在化疗前后和手术前后有明显变化。结论:肺癌患者血清TPS和CYFRA21-1阳性检出率较高,是诊断肺癌的一种敏感性强的肿瘤标志物。TPS和CYFRA21-1联合检测肺癌有重要意义。展开更多
DNA of Syngrapha falcifera nuclear polyhedrosis virus D-clone (Sfa-D clone) was extracted and digested by three kinds of restriction endonuclease. We calculated its molecular weight and measure its melting temperature...DNA of Syngrapha falcifera nuclear polyhedrosis virus D-clone (Sfa-D clone) was extracted and digested by three kinds of restriction endonuclease. We calculated its molecular weight and measure its melting temperature, G+C%. virus particle and polyhedrin were purified. The structural polypeptides and polyhedrin are analysed by SDS-PAGE.展开更多
基金supported by the Natural Science Foundation of Technology Gallery in Jilin Province of China,No.2011-15237the National Natural Science Foundation of China,No.81160159
文摘The murine microglial cell line BV2 has neuroprotective effects, but is toxic to neurons by secret-ing inlfammatory cytokines, and is an important target in the treatment of nerve inlfammation and neurodegenerative diseases. In the present study, we observed the effects of transfecting three amyloid precursor-like protein 2 （APLP2） C-terminal fragments （CTFs; C57, C50 and C31） in the pEGFP-N1 vector on S100A9 expression in BV2 cells. Reverse transcription-PCR, western blot assay and immunocytochemistry revealed that S100A9 protein and mRNA expression was greater in BV2 cells after CTF transfection than after mock transfection with an empty vector. Furthermore, transfection of full-length APLP2-751 resulted in low levels of S100A9 protein ex-pression. Our results show that APLP2-CTFs upregulate S100A9 protein and mRNA expression in BV2 cells, and identify a novel pathway involved in neuronal injury and apoptosis, and repair and protection in Alzheimer’s disease.
基金supported by the National Natural Science Foundation of China(81702015)the National Key Plan for Scientific Research and Development of China(2016YFD0500300)+6 种基金the National Science and Technology Major Project(2017ZX10303403)supported by the Excellent Young Scientist Program of the NSFC(81622031)the Excellent Young Scientist Program of the Chinese Academy of Sciences(CAS)the Youth Innovation Promotion Association CAS(2015078)supported by the Young Elite Scientist Sponsorship Program by China Association for Science and Technology(CAST)(2016QNRC001)the Youth Innovation Promotion Association CAS(2017117)a leading principal investigator of the NSFC Innovative Research Group(81621091)
文摘Dear Editor,Yellow fever（YF）,a mosquito-borne flavivirus disease,is endemic in tropical areas of Africa and Central and South America.YF is transmitted via the bite of infected Aedes aegypti or Haemogogus mosquitoes and mainly affects humans and nonhuman primates.
文摘Biological mass spectrometry has received a great progress in recent years.The osteopontin(OPN) isolated and purified from the human milk was studied by HPLC using SCX and C4 columns.After hydrolyzing the purified OPN sample by trypsin,the correlative polypeptide fragments GDSVVYGLR and QNLLAPQTLPSK were obtained by FT-ICR-MS.It showed that the method of nano-spray HPLC combined with FT-ICR-MS and Mascot search was very efficient for the analysis of the purified OPN and its polypeptide fragments.Further study provides more academic theories of their different modifications and the relative bioactivities.
文摘目的:探讨组织多肽特异性抗原(tissue polypeptide specific antigen,TPS)、细胞角蛋白19(cytokeratin19 fragment,CYFRA21-1)和鳞状细胞癌抗原(SCC-Ag)在肺癌患者血清中的表达水平及其对肺癌诊断的临床应用价值。方法:采用酶联免疫吸附法(ELISA)和免疫放射分析法(IRMA)对130例肺癌患者、67例肺部良性疾病患者及49例正常人的血清TPS,CYFRA21-1和SCC-Ag浓度进行检测。结果:肺癌组血清TPS和CYFRA21-1水平均明显高于肺部良性疾病组(P<0.01)和正常对照组(P<0.01)。TPS,CYFRA21-1和SCC-Ag阳性率与浓度均随着临床分期的进展而升高。TPS和CYFRA21-1在化疗前后和手术前后有明显变化。结论:肺癌患者血清TPS和CYFRA21-1阳性检出率较高,是诊断肺癌的一种敏感性强的肿瘤标志物。TPS和CYFRA21-1联合检测肺癌有重要意义。
文摘DNA of Syngrapha falcifera nuclear polyhedrosis virus D-clone (Sfa-D clone) was extracted and digested by three kinds of restriction endonuclease. We calculated its molecular weight and measure its melting temperature, G+C%. virus particle and polyhedrin were purified. The structural polypeptides and polyhedrin are analysed by SDS-PAGE.
