Objective To observe curative effect of electrical stimulation cerebellar fastigial nucleus on nerve function recovery of cerebral infarction patients. Method The patients were treated with electrical stimulation cere...Objective To observe curative effect of electrical stimulation cerebellar fastigial nucleus on nerve function recovery of cerebral infarction patients. Method The patients were treated with electrical stimulation cerebellar fastigial nucleus except for routine drugs, the electrode were placed on the bilateral mastoid processes,giving pulse electric current for forty minutes two times per day,for a 10 day couse treatment. The parameter was set at model 1,frequency was 131%～136%and intensity was 70%～90%.Result Total effective rate of therapeutic group and control group were 91.7%and 71.4%separately. χ2=4.860(P< 0.05).The nerver function defect score was manifestly decreased.There was a significant difference between the therapeutic group and control group,and between before and after treatment in therapeutic group(P< 0.01). Conclusion The experiment suggested that the treatment of cerebral infarction by drugs except adding electrical stimulation of cerebellar fastigial nucleus is better than by drugs along. The electrical stimulation of cerebellar fastigial nucleus has obvious protective effect.It can promote recovery of cerebral infarction.展开更多
BACKGROUND： Previous studies have confirmed that fastigial nucleus electrical stimulation can induce endogenous neuroprotective mechanisms and produce wide and long-lasting neuroprotective effects. Nevertheless, the ...BACKGROUND： Previous studies have confirmed that fastigial nucleus electrical stimulation can induce endogenous neuroprotective mechanisms and produce wide and long-lasting neuroprotective effects. Nevertheless, the precise mechanisms remain poorly understood. OBJECTIVE： This study was designed to observe the effects of fastigial nucleus electrical stimulation on nestin-positive cell expression in adult rat lateral ventricle after focal cerebral ischemia/reperfusion, as well as neurological functional changes as a function of time. DESIGN： A randomized controlled animal experiment. SETTING： Department of Neurology, First Affiliated Hospital of Chongqing Medical University; Chongqing Key Laboratory of Neurology. MATERIALS： This study was performed in the Department of Neurology, First Affiliated Hospital of Chongqing Medical University and Chongqing Key Laboratory of Neurology from September 2004 to February 2006. A total of 180 healthy, adult, male Wistar rats, aged 8 weeks old, were provided by the Laboratory Animal Center of Chongqing Medical University. The main reagents and equipments were as follows： rabbit anti-rat nestin monoclonal antibody （Wuhan Boster Company, China）. METHODS： The included male Wistar rats were randomly divided into 5 groups： normal control, sham-operated, model, fastigial nucleus sham-stimulation （sham-stimulation for short）, and fastigial nucleus electrical stimulation （stimulation for short） groups. Six time points （1 hour of ischemia and 1, 3, 7, 14, 21, and 28 days of reperfusion, 6 rats per time point） were allotted to each group. Cerebral ischemia/reperfusion was performed by occlusion to the right middle cerebral artery with suture, followed by suture removal. In the stimulation group, subsequent to reperfusion, the rat left cerebellar fastigial nucleus was immediately subjected to 1 hour of stimulation. After anesthesia, the rat left cerebellar fastigial nucleus was stimulated for 1 hour using a square-wave electronic stimulator with a current intensity of 50 μ A, frequency of 50-100 Hz, and duration of 0.5 ms. In the sham-stimulation group, the procedure was identical to the stimulation group, except the needle was retained for 1 hour and current stimulation was withdrawn. In the model group, rats were subjected to cerebral ischemia/reperfusion, but electrical stimulation was omitted. In the sham-operated group, the internal carotid artery, rather than the middle cerebral artery, was inserted with suture, and simultaneously, electrical stimulation was omitted. In the normal control group, the rats received no treatments. MAIN OUTCOME MEASURES： Nestin-positive cells were detected by immunohistochemical staining in the rat ischemic lateral cerebral ventricle at 1, 3, 7, 14, 21, and 28 days post-reperfusion. RESULTS： Morphological changes of nestin-positive cells in the ischemic lateral ventricle： in the normal control group, very few nestin-positive cells were detected in the choroid plexus, ependyma, and subependymal region of the lateral ventricle. In the model group, the number of nestin-positive cells exhibited a tendency towards a single peak, i.e., cells increased at day 1, reached peak levels by day 7, and then decreased sharply. Fastigial nucleus electrical stimulation was administered following focal cerebral ischemia/reperfusion, and results revealed that nestin-positive cell morphology was similar to horizontal cell morphology on day 7. The number of nestin-positive cells decreased after 14 days; however, the proportion of horizontal cell-like cells increased. In the sham-stimulation group, there was no change in nestin-positive cells, nestin-positive cell expression in the ischemic lateral ventricle： nestin-positive cell expression increased in the ischemic lateral cerebral ventricle, exhibiting a tendency towards unimodality; the number of cells peaked on day 7 （P 〈 0.01） and gradually decreased after 14 days （P 〈 0.01）. Following fastigial nucleus electrical stimulation, the number of nestin-positive cells increased significantly （P 〈 0.05-0.01）, reached peak levels by day 7 （P 〈 0.01）, and remained at very high levels after 14 days （P 〈 0.01）. Neurofunctional changes： neurofunctional deficits were gradually alleviated with prolonged focal cerebral ischemia/reperfusion time. At 1 hour of ischemia, and 6 hours to 7 days of reperfusion, rat neurological scores were significantly lower in the stimulation group than in the model and sham-operation groups （P 〈 0.05-0.01）. CONCLUSION： Fastigial nucleus electrical stimulation increased nestin-positive cells in the rat lateral ventricle after focal cerebral ischemia/reperfusion in a time-dependent manner and simultaneously improved neurological deficits, as well as promoted differentiation of nestin-positive cells towards a cell type with neuronal and neuroglial cellular morphology.展开更多
Objective:To explore the effect of neuromuscular electrical stimulation in treatment of acute cerebral infarction with dysphagia and psychological disorder.Methods:78 cases of patients with acute cerebral infarction w...Objective:To explore the effect of neuromuscular electrical stimulation in treatment of acute cerebral infarction with dysphagia and psychological disorder.Methods:78 cases of patients with acute cerebral infarction with dysphagia and psychological disorder were selected and randomly divided into two groups.Control group were treated with conventional drug treatment and swallowing training while neuromuscular electrical stimulation was used to additionally treat the experimental group.Profile of Mood States(Poms)and Robson self-esteem questionnaire(RSEQ-30)scores were used to evaluate the effect of neuromuscular electrical stimulation in the two groups before and after treatment.Results:In control group,both Poms and RSEQ-30 scores were not significant different before treatment.In experimental group,Poms and RSEQ-30 scores were significantly lower and higher than before treatment(P<0.05),respectively.Similarly,after treatment,Poms and RSEQ-30 scores in the experimental group were significantly lower and higher than control group(P<0.05),respectively.Conclusion:Neuromuscular electrical stimulation in treatment of acute cerebral infarction with dysphagia and psychological disorder could eliminate the patient's psychological barriers and improve their quality of life.展开更多
Following acute cerebral ischemia in rats, plasma calcitonin gene-related peptide decreased and the level of serum neuron specific enolase and the volume of the infarction increased. Square-wave and triangular-wave el...Following acute cerebral ischemia in rats, plasma calcitonin gene-related peptide decreased and the level of serum neuron specific enolase and the volume of the infarction increased. Square-wave and triangular-wave electrical stimulation with low or high intensities could increase the plasma calcitonin gene-related peptide, decrease the serum neuron specific enolase and reduce the infarction volume in the brain in rats with cerebral ischemia. There was no significant difference between different wave forms and intensities. The experimental findings indicate that low-frequency electrical stimulation with varying waveforms and intensities can treat acute cerebral ischemia in rats.展开更多
目的:分析脑电仿生电刺激仪联合丁苯酞治疗急性脑梗死的临床研究。方法:选取2020年3月至2021年5月期间本院收治的90例急性脑梗死患者作为研究对象,按随机数字表法分为对照组和观察组,各45例。对照组患者静脉滴注阿替普酶和丁苯酞治疗;...目的:分析脑电仿生电刺激仪联合丁苯酞治疗急性脑梗死的临床研究。方法:选取2020年3月至2021年5月期间本院收治的90例急性脑梗死患者作为研究对象,按随机数字表法分为对照组和观察组,各45例。对照组患者静脉滴注阿替普酶和丁苯酞治疗;观察组在对照组的基础上联合脑电仿生电刺激仪治疗。两组均治疗14 d。对比两组临床疗效、神经功能缺损评分、日常生活活动能力指数(Brathel index,BI)、肌张力评分、运动能力评分。结果:治疗后,观察组治疗总有效率明显高于对照组(P<0.05);治疗后,观察组美国国立卫生研究院卒中量表(National institutes of health stroke,NIHSS)、改良Ashworth量表(Modified Ashworth scale,MAS)评分均明显低于对照组,Brathel指数和Fugl-Meyer运动功能评分法(Fugl-Meyer Assessment,FMA)评分均明显高于对照组(P<0.05)。结论:急性脑梗死治疗中应用脑电仿生电刺激仪联合丁苯酞治疗获得较好的临床效果,改善神经功能,利于提高患者生活质量并可促进肌张力及运动能力的恢复。展开更多
文摘Objective To observe curative effect of electrical stimulation cerebellar fastigial nucleus on nerve function recovery of cerebral infarction patients. Method The patients were treated with electrical stimulation cerebellar fastigial nucleus except for routine drugs, the electrode were placed on the bilateral mastoid processes,giving pulse electric current for forty minutes two times per day,for a 10 day couse treatment. The parameter was set at model 1,frequency was 131%～136%and intensity was 70%～90%.Result Total effective rate of therapeutic group and control group were 91.7%and 71.4%separately. χ2=4.860(P< 0.05).The nerver function defect score was manifestly decreased.There was a significant difference between the therapeutic group and control group,and between before and after treatment in therapeutic group(P< 0.01). Conclusion The experiment suggested that the treatment of cerebral infarction by drugs except adding electrical stimulation of cerebellar fastigial nucleus is better than by drugs along. The electrical stimulation of cerebellar fastigial nucleus has obvious protective effect.It can promote recovery of cerebral infarction.
基金Key Technologies Research and Development Program of Chongqing Municipal Science Committee, No.2003-7Scientific Research Project of Health Bureau of Chongqing City,No.2005-2-179
文摘BACKGROUND： Previous studies have confirmed that fastigial nucleus electrical stimulation can induce endogenous neuroprotective mechanisms and produce wide and long-lasting neuroprotective effects. Nevertheless, the precise mechanisms remain poorly understood. OBJECTIVE： This study was designed to observe the effects of fastigial nucleus electrical stimulation on nestin-positive cell expression in adult rat lateral ventricle after focal cerebral ischemia/reperfusion, as well as neurological functional changes as a function of time. DESIGN： A randomized controlled animal experiment. SETTING： Department of Neurology, First Affiliated Hospital of Chongqing Medical University; Chongqing Key Laboratory of Neurology. MATERIALS： This study was performed in the Department of Neurology, First Affiliated Hospital of Chongqing Medical University and Chongqing Key Laboratory of Neurology from September 2004 to February 2006. A total of 180 healthy, adult, male Wistar rats, aged 8 weeks old, were provided by the Laboratory Animal Center of Chongqing Medical University. The main reagents and equipments were as follows： rabbit anti-rat nestin monoclonal antibody （Wuhan Boster Company, China）. METHODS： The included male Wistar rats were randomly divided into 5 groups： normal control, sham-operated, model, fastigial nucleus sham-stimulation （sham-stimulation for short）, and fastigial nucleus electrical stimulation （stimulation for short） groups. Six time points （1 hour of ischemia and 1, 3, 7, 14, 21, and 28 days of reperfusion, 6 rats per time point） were allotted to each group. Cerebral ischemia/reperfusion was performed by occlusion to the right middle cerebral artery with suture, followed by suture removal. In the stimulation group, subsequent to reperfusion, the rat left cerebellar fastigial nucleus was immediately subjected to 1 hour of stimulation. After anesthesia, the rat left cerebellar fastigial nucleus was stimulated for 1 hour using a square-wave electronic stimulator with a current intensity of 50 μ A, frequency of 50-100 Hz, and duration of 0.5 ms. In the sham-stimulation group, the procedure was identical to the stimulation group, except the needle was retained for 1 hour and current stimulation was withdrawn. In the model group, rats were subjected to cerebral ischemia/reperfusion, but electrical stimulation was omitted. In the sham-operated group, the internal carotid artery, rather than the middle cerebral artery, was inserted with suture, and simultaneously, electrical stimulation was omitted. In the normal control group, the rats received no treatments. MAIN OUTCOME MEASURES： Nestin-positive cells were detected by immunohistochemical staining in the rat ischemic lateral cerebral ventricle at 1, 3, 7, 14, 21, and 28 days post-reperfusion. RESULTS： Morphological changes of nestin-positive cells in the ischemic lateral ventricle： in the normal control group, very few nestin-positive cells were detected in the choroid plexus, ependyma, and subependymal region of the lateral ventricle. In the model group, the number of nestin-positive cells exhibited a tendency towards a single peak, i.e., cells increased at day 1, reached peak levels by day 7, and then decreased sharply. Fastigial nucleus electrical stimulation was administered following focal cerebral ischemia/reperfusion, and results revealed that nestin-positive cell morphology was similar to horizontal cell morphology on day 7. The number of nestin-positive cells decreased after 14 days; however, the proportion of horizontal cell-like cells increased. In the sham-stimulation group, there was no change in nestin-positive cells, nestin-positive cell expression in the ischemic lateral ventricle： nestin-positive cell expression increased in the ischemic lateral cerebral ventricle, exhibiting a tendency towards unimodality; the number of cells peaked on day 7 （P 〈 0.01） and gradually decreased after 14 days （P 〈 0.01）. Following fastigial nucleus electrical stimulation, the number of nestin-positive cells increased significantly （P 〈 0.05-0.01）, reached peak levels by day 7 （P 〈 0.01）, and remained at very high levels after 14 days （P 〈 0.01）. Neurofunctional changes： neurofunctional deficits were gradually alleviated with prolonged focal cerebral ischemia/reperfusion time. At 1 hour of ischemia, and 6 hours to 7 days of reperfusion, rat neurological scores were significantly lower in the stimulation group than in the model and sham-operation groups （P 〈 0.05-0.01）. CONCLUSION： Fastigial nucleus electrical stimulation increased nestin-positive cells in the rat lateral ventricle after focal cerebral ischemia/reperfusion in a time-dependent manner and simultaneously improved neurological deficits, as well as promoted differentiation of nestin-positive cells towards a cell type with neuronal and neuroglial cellular morphology.
文摘Objective:To explore the effect of neuromuscular electrical stimulation in treatment of acute cerebral infarction with dysphagia and psychological disorder.Methods:78 cases of patients with acute cerebral infarction with dysphagia and psychological disorder were selected and randomly divided into two groups.Control group were treated with conventional drug treatment and swallowing training while neuromuscular electrical stimulation was used to additionally treat the experimental group.Profile of Mood States(Poms)and Robson self-esteem questionnaire(RSEQ-30)scores were used to evaluate the effect of neuromuscular electrical stimulation in the two groups before and after treatment.Results:In control group,both Poms and RSEQ-30 scores were not significant different before treatment.In experimental group,Poms and RSEQ-30 scores were significantly lower and higher than before treatment(P<0.05),respectively.Similarly,after treatment,Poms and RSEQ-30 scores in the experimental group were significantly lower and higher than control group(P<0.05),respectively.Conclusion:Neuromuscular electrical stimulation in treatment of acute cerebral infarction with dysphagia and psychological disorder could eliminate the patient's psychological barriers and improve their quality of life.
基金the National High-Tech R&D Program of China (863 Program),No.2007AA022Z482
文摘Following acute cerebral ischemia in rats, plasma calcitonin gene-related peptide decreased and the level of serum neuron specific enolase and the volume of the infarction increased. Square-wave and triangular-wave electrical stimulation with low or high intensities could increase the plasma calcitonin gene-related peptide, decrease the serum neuron specific enolase and reduce the infarction volume in the brain in rats with cerebral ischemia. There was no significant difference between different wave forms and intensities. The experimental findings indicate that low-frequency electrical stimulation with varying waveforms and intensities can treat acute cerebral ischemia in rats.
文摘目的:分析脑电仿生电刺激仪联合丁苯酞治疗急性脑梗死的临床研究。方法:选取2020年3月至2021年5月期间本院收治的90例急性脑梗死患者作为研究对象,按随机数字表法分为对照组和观察组,各45例。对照组患者静脉滴注阿替普酶和丁苯酞治疗;观察组在对照组的基础上联合脑电仿生电刺激仪治疗。两组均治疗14 d。对比两组临床疗效、神经功能缺损评分、日常生活活动能力指数(Brathel index,BI)、肌张力评分、运动能力评分。结果:治疗后,观察组治疗总有效率明显高于对照组(P<0.05);治疗后,观察组美国国立卫生研究院卒中量表(National institutes of health stroke,NIHSS)、改良Ashworth量表(Modified Ashworth scale,MAS)评分均明显低于对照组,Brathel指数和Fugl-Meyer运动功能评分法(Fugl-Meyer Assessment,FMA)评分均明显高于对照组(P<0.05)。结论:急性脑梗死治疗中应用脑电仿生电刺激仪联合丁苯酞治疗获得较好的临床效果,改善神经功能,利于提高患者生活质量并可促进肌张力及运动能力的恢复。
