本文用胆盐羧苄青霉素及EDTA诱导侵袭性大肠埃希氏菌(Enteroinvasive E. coil下简称EIEC)成为缺壁浓染的长丝体及典型的L型菌落。通过其生物学特性观察,我们发现这株L型始终在普通等渗培养基上生长并容易恢复,并发现这些特性的改变与细...本文用胆盐羧苄青霉素及EDTA诱导侵袭性大肠埃希氏菌(Enteroinvasive E. coil下简称EIEC)成为缺壁浓染的长丝体及典型的L型菌落。通过其生物学特性观察,我们发现这株L型始终在普通等渗培养基上生长并容易恢复,并发现这些特性的改变与细胞壁缺陷程度密切相关。本文对G^-菌L型的诱导以及在临床细菌检验中的意义等问题进行了讨论。展开更多
A multiplex polymerase chain reaction （PCR） was developed to detect three pathogenic genes of enteropathogenic, enterotocigenic and enteroinvasive Escherichia coli In this study three different sets of oligonucleoti...A multiplex polymerase chain reaction （PCR） was developed to detect three pathogenic genes of enteropathogenic, enterotocigenic and enteroinvasive Escherichia coli In this study three different sets of oligonucleotide primer were simultaneously used, and in this way, specific fragments of 880, 600, 150 bp for EPEC eaeA, EIEC ipaH and ETEC ST genes were amplified, respectively. The best condition of the multiplex PCR was： after an initial heat denaturation step at 95℃for 5 min, followed by 30 cycles of denaturation at 94 ℃ for 40 s, primer annealing at 51.3℃ for 40 s and extension at 72 ℃ for 1 min, final extension at 72 ℃ for 10 min. The detection limit of the eaeA, ipaH and ST primers was 38.7423, 3.60519, 29.9448 ng·mL^-1 （4.3×10^4, 1.5×10^3, 2.6×10^4 CFU·mL^-1）, respectively. It may be a good way for the detection and identification of Diarrhea-causing E. coli.展开更多
文摘本文用胆盐羧苄青霉素及EDTA诱导侵袭性大肠埃希氏菌(Enteroinvasive E. coil下简称EIEC)成为缺壁浓染的长丝体及典型的L型菌落。通过其生物学特性观察,我们发现这株L型始终在普通等渗培养基上生长并容易恢复,并发现这些特性的改变与细胞壁缺陷程度密切相关。本文对G^-菌L型的诱导以及在临床细菌检验中的意义等问题进行了讨论。
基金Key Item of National Technology Research Project (2002BA518A06)Heilongjiang Province Department Fund (10541021)
文摘A multiplex polymerase chain reaction （PCR） was developed to detect three pathogenic genes of enteropathogenic, enterotocigenic and enteroinvasive Escherichia coli In this study three different sets of oligonucleotide primer were simultaneously used, and in this way, specific fragments of 880, 600, 150 bp for EPEC eaeA, EIEC ipaH and ETEC ST genes were amplified, respectively. The best condition of the multiplex PCR was： after an initial heat denaturation step at 95℃for 5 min, followed by 30 cycles of denaturation at 94 ℃ for 40 s, primer annealing at 51.3℃ for 40 s and extension at 72 ℃ for 1 min, final extension at 72 ℃ for 10 min. The detection limit of the eaeA, ipaH and ST primers was 38.7423, 3.60519, 29.9448 ng·mL^-1 （4.3×10^4, 1.5×10^3, 2.6×10^4 CFU·mL^-1）, respectively. It may be a good way for the detection and identification of Diarrhea-causing E. coli.
