Embryonic stem cells （ESCs） hold great promises for treating and studying numerous devastating diseases. The molecular basis of their potential is not completely understood. Large noncoding RNAs （lncRNAs） are an i...Embryonic stem cells （ESCs） hold great promises for treating and studying numerous devastating diseases. The molecular basis of their potential is not completely understood. Large noncoding RNAs （lncRNAs） are an important class of gene regulators that play essential roles in a variety of physiologic and pathologic processes. Dozens of lncRNAs are now identified to control ESC self-renewal and differentiation. Research on lncRNAs may provide novel insights into manipulating the cell fate or reprogramming somatic cells into induced pluripotent stem cells （iPSCs）. In this review, we summarize the recent research efforts in identifying functional lncRNAs and understanding how they act in ESCs, and discuss various future directions of this field.展开更多
ObjectiveTo investigate BCL 6 gene mutations in Chinese populations with B cell non Hodgkin's lymphoma Methods Polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and direct D...ObjectiveTo investigate BCL 6 gene mutations in Chinese populations with B cell non Hodgkin's lymphoma Methods Polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and direct DNA sequencing were used to identify mutations in the 5' noncoding region of the BCL 6 gene in a total of 40 cases of diffuse large cell lymphoma (DLCL) and follicular lymphoma (FL) Results Nine cases were found to have base substitutions The incidence of BCL 6 gene mutation and the frequency of single base changes were approximately 25 7% and (0 56-1 10)×10 2 /bp, respectively Conclusions The 5' regulatory region of the BCL 6 gene undergoes frequent somatic hypermutation during lymphomagenesis and the identification of BCL 6 gene hypermutations provides a molecular marker for confirmatory diagnosis of B NHL展开更多
基金supported by grants from Chinese Ministry of Science and Technology (Nos. 2011CBA01100 and 2012CB966700)the National Natural Science Foundation of China (No. 31221002)
文摘Embryonic stem cells （ESCs） hold great promises for treating and studying numerous devastating diseases. The molecular basis of their potential is not completely understood. Large noncoding RNAs （lncRNAs） are an important class of gene regulators that play essential roles in a variety of physiologic and pathologic processes. Dozens of lncRNAs are now identified to control ESC self-renewal and differentiation. Research on lncRNAs may provide novel insights into manipulating the cell fate or reprogramming somatic cells into induced pluripotent stem cells （iPSCs）. In this review, we summarize the recent research efforts in identifying functional lncRNAs and understanding how they act in ESCs, and discuss various future directions of this field.
基金ThisworkwassupportedbythegrantsfromtheNationalNaturalScienceFoundationofChina (No 3 9770 83 0 ) andDr ClydeFoundation
文摘ObjectiveTo investigate BCL 6 gene mutations in Chinese populations with B cell non Hodgkin's lymphoma Methods Polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and direct DNA sequencing were used to identify mutations in the 5' noncoding region of the BCL 6 gene in a total of 40 cases of diffuse large cell lymphoma (DLCL) and follicular lymphoma (FL) Results Nine cases were found to have base substitutions The incidence of BCL 6 gene mutation and the frequency of single base changes were approximately 25 7% and (0 56-1 10)×10 2 /bp, respectively Conclusions The 5' regulatory region of the BCL 6 gene undergoes frequent somatic hypermutation during lymphomagenesis and the identification of BCL 6 gene hypermutations provides a molecular marker for confirmatory diagnosis of B NHL
