BACKGROUND Type 2 diabetes mellitus is characterized by pancreaticβ-cell dysfunction and insulin resistance.Studies have suggested thatβ-cell dedifferentiation is one of the pathogeneses ofβ-cell dysfunction,but th...BACKGROUND Type 2 diabetes mellitus is characterized by pancreaticβ-cell dysfunction and insulin resistance.Studies have suggested thatβ-cell dedifferentiation is one of the pathogeneses ofβ-cell dysfunction,but the detailed mechanism is still unclear.Most studies ofβ-cell dedifferentiation rely on rodent models and human pathological specimens.The development of in vitro systems can facilitate the exploration ofβ-cell dedifferentiation.AIM To investigate the molecular mechanism ofβ-cell dedifferentiation.Hence,an in vitro model ofβ-cell dedifferentiation induced by palmitic acid and high glucose was established using the INS-1832/13 cell line.METHODS The study was further analyzed using RNA-sequencing,transmission electron microscopy,quantitative real-time polymerase chain reaction and Western blot.RESULTS Results showed that the treatment of palmitic acid and high glucose significantly up-regulatedβ-cell forbidden genes and endocrine precursor cell marker genes,and down-regulated the expression ofβ-cell specific markers.Data showed that dedifferentiated INS-1 cells up-regulated the expression of endoplasmic reticulum(ER)stressrelated genes.Moreover,the results also showed that forkhead box O1(Foxo1)inhibition potentiated genetic changes inβ-cell dedifferentiation induced by palmitic acid and high glucose.CONCLUSION ER stress is sufficient to triggerβ-cell dedifferentiation and is necessary for palmitic acid and high glucose-inducedβ-cell dedifferentiation.Foxo1 inhibition can further enhance these phenomena.展开更多
BACKGROUND Centromere protein A(CENPA)exhibits an increased expression level in primary human rectal cancer tissues,but its role has not been investigated.AIM To clarify the specific role and mechanism of CENPA in rec...BACKGROUND Centromere protein A(CENPA)exhibits an increased expression level in primary human rectal cancer tissues,but its role has not been investigated.AIM To clarify the specific role and mechanism of CENPA in rectal cancer progression.METHODS CENPA protein expression in rectal cancer tissues and cell lines were detected.CENPA was overexpressed and knocked down in SW837 and SW480 cells,and proliferation,invasion,apoptosis and epithelial-mesenchymal transition(EMT)marker protein levels were examined.O6-methylguanine DNA methyltransferase(MGMT)promoter methylation was assessed with methylation-specific poly-merase chain reaction.Co-immunoprecipitation assay verified the interaction between MGMT and protein tyrosine phosphatase nonreceptor type 4(PTPN4).SW837 cells with CENPA knockdown were injected subcutaneously into mice,and tumor growth was examined.RESULTS CENPA was upregulated in rectal cancer tissues and cell lines.CENPA overex-pression promoted proliferation,invasion and EMT,and inhibited apoptosis in rectal cancer cells.Whereas CENPA knockdown showed the opposite results.Moreover,CENPA inhibited MGMT expression by promoting DNA methyltrans-ferase 1-mediated MGMT promoter methylation.MGMT knockdown abolished the CENPA knockdown-mediated inhibition of rectal cancer cell progression.MGMT increased PTPN4 protein stability by inhibiting PTPN4 ubiquitination degradation via competing with ubiquitin-conjugating enzyme E2O for interacting with PTPN4.PTPN4 knockdown abolished the inhibitory effects of MGMT overexpression on rectal cancer cell progression.Moreover,CENPA knockdown inhibited xenograft tumor growth in vivo.CONCLUSION CENPA knockdown inhibited rectal cancer cell growth and attenuated xenograft tumor growth through regulating the MGMT/PTPN4 axis.展开更多
To construct high-performance aqueous ammonium-ion full batteries,(NH_(4))_(2)V_(6)O_(16)·1.5H_(2)O(NVO)nanoribbon cathodes were prepared by pH-regulated hydrothermal synthesis.Anodes were prepared by growing the...To construct high-performance aqueous ammonium-ion full batteries,(NH_(4))_(2)V_(6)O_(16)·1.5H_(2)O(NVO)nanoribbon cathodes were prepared by pH-regulated hydrothermal synthesis.Anodes were prepared by growing the active material polyaniline(PANI)on carbon cloth.The assembled NVO//PANI full cells exhibit a reversible capacity of 109.5 mA·h/g at a current density of 1.0 A/g and a high energy density of 23 W·h/kg.The ammonium-ion intercalation/extraction mechanism is primarily governed by the pseudocapacitance behavior.These results indicate that NVO is a potential candidate as a cathode material for aqueous ammonium-ion batteries.展开更多
在室内可见光通信中符号间干扰和噪声会严重影响系统性能,K均值(K-means)均衡方法可以抑制光无线信道的影响,但其复杂度较高,且在聚类边界处易出现误判。提出了改进聚类中心点的K-means(Improved Center K-means,IC-Kmeans)算法,通过随...在室内可见光通信中符号间干扰和噪声会严重影响系统性能,K均值(K-means)均衡方法可以抑制光无线信道的影响,但其复杂度较高,且在聚类边界处易出现误判。提出了改进聚类中心点的K-means(Improved Center K-means,IC-Kmeans)算法,通过随机生成足够长的训练序列,然后将训练序列每一簇的均值作为K-means聚类中心,避免了传统K-means反复迭代寻找聚类中心。进一步,提出了基于神经网络的IC-Kmeans(Neural Network Based IC-Kmeans,NNIC-Kmeans)算法,使用反向传播神经网络将接收端二维数据映射至三维空间,以增加不同簇之间混合数据的距离,提高了分类准确性。蒙特卡罗误码率仿真表明,IC-Kmeans均衡和传统K-means算法的误码率性能相当,但可以显著降低复杂度,特别是在信噪比较小时。同时,在室内多径信道模型下,与IC-Kmeans和传统Kmeans均衡相比,NNIC-Kmeans均衡的光正交频分复用系统误码率性能最好。展开更多
基金Supported by the Natural Science Foundation of China,No.81471081the Natural Science Foundation of Fujian Province,No.2023D009+1 种基金the Natural Science Foundation of Xiamen City,No.3502Z202373104 and No.3502Z20227162Scientific Research Foundation for Advanced Talents,Xiang’an Hospital of Xiamen University,No.PM201809170005。
文摘BACKGROUND Type 2 diabetes mellitus is characterized by pancreaticβ-cell dysfunction and insulin resistance.Studies have suggested thatβ-cell dedifferentiation is one of the pathogeneses ofβ-cell dysfunction,but the detailed mechanism is still unclear.Most studies ofβ-cell dedifferentiation rely on rodent models and human pathological specimens.The development of in vitro systems can facilitate the exploration ofβ-cell dedifferentiation.AIM To investigate the molecular mechanism ofβ-cell dedifferentiation.Hence,an in vitro model ofβ-cell dedifferentiation induced by palmitic acid and high glucose was established using the INS-1832/13 cell line.METHODS The study was further analyzed using RNA-sequencing,transmission electron microscopy,quantitative real-time polymerase chain reaction and Western blot.RESULTS Results showed that the treatment of palmitic acid and high glucose significantly up-regulatedβ-cell forbidden genes and endocrine precursor cell marker genes,and down-regulated the expression ofβ-cell specific markers.Data showed that dedifferentiated INS-1 cells up-regulated the expression of endoplasmic reticulum(ER)stressrelated genes.Moreover,the results also showed that forkhead box O1(Foxo1)inhibition potentiated genetic changes inβ-cell dedifferentiation induced by palmitic acid and high glucose.CONCLUSION ER stress is sufficient to triggerβ-cell dedifferentiation and is necessary for palmitic acid and high glucose-inducedβ-cell dedifferentiation.Foxo1 inhibition can further enhance these phenomena.
基金This study was reviewed and approved by the Ethic Committee of Medical College of Henan Vocational University of Science and Technology(Approval No.HVUYL414101416920231017001)all participants signed a written informed consent.
文摘BACKGROUND Centromere protein A(CENPA)exhibits an increased expression level in primary human rectal cancer tissues,but its role has not been investigated.AIM To clarify the specific role and mechanism of CENPA in rectal cancer progression.METHODS CENPA protein expression in rectal cancer tissues and cell lines were detected.CENPA was overexpressed and knocked down in SW837 and SW480 cells,and proliferation,invasion,apoptosis and epithelial-mesenchymal transition(EMT)marker protein levels were examined.O6-methylguanine DNA methyltransferase(MGMT)promoter methylation was assessed with methylation-specific poly-merase chain reaction.Co-immunoprecipitation assay verified the interaction between MGMT and protein tyrosine phosphatase nonreceptor type 4(PTPN4).SW837 cells with CENPA knockdown were injected subcutaneously into mice,and tumor growth was examined.RESULTS CENPA was upregulated in rectal cancer tissues and cell lines.CENPA overex-pression promoted proliferation,invasion and EMT,and inhibited apoptosis in rectal cancer cells.Whereas CENPA knockdown showed the opposite results.Moreover,CENPA inhibited MGMT expression by promoting DNA methyltrans-ferase 1-mediated MGMT promoter methylation.MGMT knockdown abolished the CENPA knockdown-mediated inhibition of rectal cancer cell progression.MGMT increased PTPN4 protein stability by inhibiting PTPN4 ubiquitination degradation via competing with ubiquitin-conjugating enzyme E2O for interacting with PTPN4.PTPN4 knockdown abolished the inhibitory effects of MGMT overexpression on rectal cancer cell progression.Moreover,CENPA knockdown inhibited xenograft tumor growth in vivo.CONCLUSION CENPA knockdown inhibited rectal cancer cell growth and attenuated xenograft tumor growth through regulating the MGMT/PTPN4 axis.
基金supported by the National Natural Science Foundation of China(Nos.52171200,52371211)the Changsha Special Project,China(No.kh2301006)。
文摘To construct high-performance aqueous ammonium-ion full batteries,(NH_(4))_(2)V_(6)O_(16)·1.5H_(2)O(NVO)nanoribbon cathodes were prepared by pH-regulated hydrothermal synthesis.Anodes were prepared by growing the active material polyaniline(PANI)on carbon cloth.The assembled NVO//PANI full cells exhibit a reversible capacity of 109.5 mA·h/g at a current density of 1.0 A/g and a high energy density of 23 W·h/kg.The ammonium-ion intercalation/extraction mechanism is primarily governed by the pseudocapacitance behavior.These results indicate that NVO is a potential candidate as a cathode material for aqueous ammonium-ion batteries.
文摘在室内可见光通信中符号间干扰和噪声会严重影响系统性能,K均值(K-means)均衡方法可以抑制光无线信道的影响,但其复杂度较高,且在聚类边界处易出现误判。提出了改进聚类中心点的K-means(Improved Center K-means,IC-Kmeans)算法,通过随机生成足够长的训练序列,然后将训练序列每一簇的均值作为K-means聚类中心,避免了传统K-means反复迭代寻找聚类中心。进一步,提出了基于神经网络的IC-Kmeans(Neural Network Based IC-Kmeans,NNIC-Kmeans)算法,使用反向传播神经网络将接收端二维数据映射至三维空间,以增加不同簇之间混合数据的距离,提高了分类准确性。蒙特卡罗误码率仿真表明,IC-Kmeans均衡和传统K-means算法的误码率性能相当,但可以显著降低复杂度,特别是在信噪比较小时。同时,在室内多径信道模型下,与IC-Kmeans和传统Kmeans均衡相比,NNIC-Kmeans均衡的光正交频分复用系统误码率性能最好。
