BACKGROUND Mesenchymal stem cells(MSCs)modulated by various exogenous signals have been applied extensively in regenerative medicine research.Notably,nanosecond pulsed electric fields(nsPEFs),characterized by short du...BACKGROUND Mesenchymal stem cells(MSCs)modulated by various exogenous signals have been applied extensively in regenerative medicine research.Notably,nanosecond pulsed electric fields(nsPEFs),characterized by short duration and high strength,significantly influence cell phenotypes and regulate MSCs differentiation via multiple pathways.Consequently,we used transcriptomics to study changes in messenger RNA(mRNA),long noncoding RNA(lncRNA),microRNA(miRNA),and circular RNA expression during nsPEFs application.AIM To explore gene expression profiles and potential transcriptional regulatory mechanisms in MSCs pretreated with nsPEFs.METHODS The impact of nsPEFs on the MSCs transcriptome was investigated through whole transcriptome sequencing.MSCs were pretreated with 5-pulse nsPEFs(100 ns at 10 kV/cm,1 Hz),followed by total RNA isolation.Each transcript was normalized by fragments per kilobase per million.Fold change and difference significance were applied to screen the differentially expressed genes(DEGs).Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to elucidate gene functions,complemented by quantitative polymerase chain reaction verification.RESULTS In total,263 DEGs were discovered,with 92 upregulated and 171 downregulated.DEGs were predominantly enriched in epithelial cell proliferation,osteoblast differentiation,mesenchymal cell differentiation,nuclear division,and wound healing.Regarding cellular components,DEGs are primarily involved in condensed chromosome,chromosomal region,actin cytoskeleton,and kinetochore.From aspect of molecular functions,DEGs are mainly involved in glycosaminoglycan binding,integrin binding,nuclear steroid receptor activity,cytoskeletal motor activity,and steroid binding.Quantitative real-time polymerase chain reaction confirmed targeted transcript regulation.CONCLUSION Our systematic investigation of the wide-ranging transcriptional pattern modulated by nsPEFs revealed the differential expression of 263 mRNAs,2 miRNAs,and 65 lncRNAs.Our study demonstrates that nsPEFs may affect stem cells through several signaling pathways,which are involved in vesicular transport,calcium ion transport,cytoskeleton,and cell differentiation.展开更多
This paper studies the dynamic responses of SDOF system under pulse-dominant excitations.The purpose of the study is to prepare for scrutiny of some near-field pulse-dominantground motions and their potential to cause...This paper studies the dynamic responses of SDOF system under pulse-dominant excitations.The purpose of the study is to prepare for scrutiny of some near-field pulse-dominantground motions and their potential to cause structural damage.Extending the single pulse dynamics,we consider the effect of pulse sequences.This kind of excitation was particularly obvious in some of previous earthquakes such as Northridge (1994) and Chi-Chi (1995).Based on the duration, peak and rise and decay era of the main pulse as well as its relationship with the predecessor and successor pulses,we propose a classification for the pulse sequences.Consequent studies have been canied out for acceleration,velocity and displacement response spectra of the main pulse with either a predecessor or a successor pulse.The analysis also includes general response behaviors in different fundamental period segments and special aspects of response at certain points (e.g.,the corresponding peak points).展开更多
There is a large amount of fat in the postganglionic segment of the brachial plexus nerve.The use of short T1 inversion recovery pulse sequence may improve signal strength of the brachial plexus postganglionic segment...There is a large amount of fat in the postganglionic segment of the brachial plexus nerve.The use of short T1 inversion recovery pulse sequence may improve signal strength of the brachial plexus postganglionic segment.The present study revealed that the combination of three-dimensional fast imaging employing steady-state acquisition with phase-cycled and short T1 inversion recovery pulse sequence clearly displayed the anatomical morphology and structure of the brachial plexus nerve,together with maximum intensity projection,volume rendering and other three-dimensional reconstruction techniques.Our results suggested that this method is also suitable for providing accurate assessment and diagnosis of the site,severity and scope of brachial plexus injury.展开更多
A modified DBSCAN algorithm is presented for deinterleaving of radar pulses in modern EW environments.A main characteristic of the proposed method is that using only time of arrival of pulses,the method can sort the p...A modified DBSCAN algorithm is presented for deinterleaving of radar pulses in modern EW environments.A main characteristic of the proposed method is that using only time of arrival of pulses,the method can sort the pulses efficiently.Other PDW information such as rise time,carrier frequency,pulse width,modulation on pulse,fall time and direction of arrival are not required.To identify the valid PRIs in a set of interleaved pulses,an innovative modification of the DBSCAN algorithm is introduced which is accurate and easy to implement.The proposed method determines valid PRIs more accurately and neglects the spurious ones more efficiently as compared to the classical histogram based algorithms such as SDIF.Furthermore,without specifying any input parameter,the proposed method can deinterleave radar pulses while up to 30%jitter is present in the associated PRI.The accuracy and efficiency of the proposed method are verified by computer simulations and real data results.Experimental simulations are based on different real and operational scenarios where the presence of missing and spurious pulses are also considered.So,the simulation results can be of practical significance.展开更多
On the basis of the relationship between the Hamiltonian of spin 1/2 quantum system under control and the energy level structure and transitions, a radio frequency pulse sequence is designed using intuitive and half c...On the basis of the relationship between the Hamiltonian of spin 1/2 quantum system under control and the energy level structure and transitions, a radio frequency pulse sequence is designed using intuitive and half counter-intuitive sequences of pulse to transfer the population of the 3-qubit system coherently. The effectiveness of the designed control sequence is verified through the system simulation experiment of the evolution of state. In principle, the design method of the control pulse sequence proposed can be generalized to use in the quantum systems of higher dimension.展开更多
The study of phased array radar( PAR) pulse amplitude sequence characteristics is the key to understand the radar's working state and its beam's scanning manner.According to the principle of antenna pattern fo...The study of phased array radar( PAR) pulse amplitude sequence characteristics is the key to understand the radar's working state and its beam's scanning manner.According to the principle of antenna pattern formation and the searching and tracking modes of beams,this paper analyzes the characteristics and differences of pulse amplitude sequence when the radar beams work in searching and tracking modes respectively.Then an optimal sorting model of pulse amplitude sequence is established based on least-squares and curve-fitting methods.This method is helpful for acquiring the current working state of the radar and recognizing its instantaneous beam pointing by sorting the pulse amplitude sequence without the necessity to estimate the antenna pattern.展开更多
Pulse repetition interval(PRI)modulation recognition and pulse sequence search are significant for effective electronic support measures.In modern electromagnetic environments,different types of inter-pulse slide rada...Pulse repetition interval(PRI)modulation recognition and pulse sequence search are significant for effective electronic support measures.In modern electromagnetic environments,different types of inter-pulse slide radars are highly confusing.There are few available training samples in practical situations,which leads to a low recognition accuracy and poor search effect of the pulse sequence.In this paper,an approach based on bi-directional long short-term memory(BiLSTM)networks and the temporal correlation algorithm for PRI modulation recognition and sequence search under the small sample prerequisite is proposed.The simulation results demonstrate that the proposed algorithm can recognize unilinear,bilinear,sawtooth,and sinusoidal PRI modulation types with 91.43% accuracy and complete the pulse sequence search with 30% missing pulses and 50% spurious pulses under the small sample prerequisite.展开更多
The group correlation properties of binary sequences is studied, and a conclusion is drawn that not only the group correlation function of a binary sequence itself is ideal, but also some of its subsets, which code le...The group correlation properties of binary sequences is studied, and a conclusion is drawn that not only the group correlation function of a binary sequence itself is ideal, but also some of its subsets, which code length N is even, is ideal. Finally a general formula of the group correlation of a binary sequence set is derived.展开更多
In this framework we present a new method for measurement of the UWB impulse train based on the parallel sampling of the cascaded identical RC filters. We show that the amplitudes and time locations of p sequential im...In this framework we present a new method for measurement of the UWB impulse train based on the parallel sampling of the cascaded identical RC filters. We show that the amplitudes and time locations of p sequential impulses can be reconstructed from simultaneous measurement of the outputs from 2p cascaded identical RC filters. The parallel sampling scheme has a wide range of applications including the detection of the ultra wideband (UWB) impulses. Due to identical analog RC filters and buffer amplifiers, the parallel sampling scheme is flexible to implement in VLSI applications.展开更多
The contrast agent concentration, the time of repetition (TR) and magnetic field strength are significant parameters that influence for the accurate signal intensity (SI) in quantitative Magnetic Resonance Imaging (MR...The contrast agent concentration, the time of repetition (TR) and magnetic field strength are significant parameters that influence for the accurate signal intensity (SI) in quantitative Magnetic Resonance Imaging (MRI). Therefore, this study was conducted to investigate and refine the dependence and the optimal effect of Time of Repetition (TR) on the relationship between signal intensity and Gd-DTPA (Gadolinium-diethylene-triaminepenta-acetic acid) concentration, after applying two-dimensional (2D) Spin Echo (SE) pulse sequence under low-field MRI. In addition to that, the optimal concentration of Gd-DTPA at given sequence parameters at low-field MRI was also evaluated. A water-filled phantom was constructed for a range of Gd-DTPA concentrations (0 - 6 mmol/L) and the mean signal intensities (SIs) were assessed in the defined region of interest on T1-weighted images with different TR values (40 - 2000 ms). The generated signal-concentration curves for Gd-DTPA revealed that increasing TR was associated with the increase of the overall SIs and the maximum relationship between SI to concentration. Moreover, the required Gd-DTPA concentration to produce the maximum SI was associated to decrease with the increase of TR. In addition to this, the application of beyond 100 ms TR values in this study with relatively higher concentrations (beyond 1 - 2 mmol/L) has resulted predominantly non-linear patterns in the signal-concentration curves and it appears the saturation or decay of the SIs due to T2 effect. From these results, it can be suggested that the selection of relatively lower Gd-DTPA concentration ( mmol/L) with less than 800 ms (<800 ms) TR values can produce a better linear relationship between the concertation and SIs in T1-weighted SE low field contrast-enhanced MRI. Furthermore, this study also outlined the significance and necessity of the optimization of TR in SE sequence in low field MRI prior to a particular examination.展开更多
Ribonucleic acid(RNA)structures and dynamics play a crucial role in elucidating RNA functions and facilitating the design of drugs targeting RNA and RNA-protein complexes.However,obtaining RNA structures using convent...Ribonucleic acid(RNA)structures and dynamics play a crucial role in elucidating RNA functions and facilitating the design of drugs targeting RNA and RNA-protein complexes.However,obtaining RNA structures using conventional biophysical techniques,such as Xray crystallography and solution nuclear magnetic resonance(NMR),presents challenges due to the inherent flexibility and susceptibility to degradation of RNA.In recent years,solid-state NMR(SSNMR)has rapidly emerged as a promising alternative technique for characterizing RNA structure and dynamics.SSNMR has several distinct advantages,including flexibility in sample states,the ability to capture dynamic features of RNA in solid form,and suitability to character RNAs in various sizes.Recent decade witnessed the growth of ^(1)H-detected SSNMR methods on RNA,which targeted elucidating RNA topology and base pair dynamics in solid state.They have been applied to determine the topology of RNA segment in human immunodeficiency virus(HIV)genome and the base pair dynamics of riboswitch RNA.These advancements have expanded the utility of SSNMR techniques within the RNA research field.This review provides a comprehensive discussion of recent progress in ^(1)H-detected SSNMR investigations into RNA structure and dynamics.We focus on the established ^(1)H-detected SSNMR methods,sample preparation protocols,and the implementation of rapid data acquisition approaches.展开更多
本文主要探讨了大功率三电平应用中3.3 kV SiC MOSFET和IGBT的混合应用问题,针对外管高频、内管低频的调制策略中不同箝位管脉冲时序对三电平有源中点箝位(ANPC)拓扑换流路径、开关损耗以及开关特性等关键因素的影响开展分析,实验验证...本文主要探讨了大功率三电平应用中3.3 kV SiC MOSFET和IGBT的混合应用问题,针对外管高频、内管低频的调制策略中不同箝位管脉冲时序对三电平有源中点箝位(ANPC)拓扑换流路径、开关损耗以及开关特性等关键因素的影响开展分析,实验验证了不同箝位管脉冲时序对SiC MOSFET和IGBT的动态特性和开关损耗的影响。展开更多
It is an efficient method to model ship radiated noise as quasi-periodic random sound pulse sequences. Based on the model, this paper discusses the characteristics change of ship noise after through shallow-water wave...It is an efficient method to model ship radiated noise as quasi-periodic random sound pulse sequences. Based on the model, this paper discusses the characteristics change of ship noise after through shallow-water waveguides. Theoretical analysis and numerical simulation show that random waveguides and multi-path effects can bring much additional transmission loss for the noise line spectra, demonstrating the instability of ship radiated noise line spectra to a certain extent, and providing some theoretical support for advanced studies of ship radiated noise.展开更多
基金Supported by the National Natural Science Foundation,China,No.82272568,81902247,and 32201013Natural Science Foundation of Shandong Province,China,No.ZR2021QH275+1 种基金Natural Science Foundation of Jinan City,China,No.202225070Guangdong Basic and Applied Basic Research Foundation,China,No.2022A1515220056.
文摘BACKGROUND Mesenchymal stem cells(MSCs)modulated by various exogenous signals have been applied extensively in regenerative medicine research.Notably,nanosecond pulsed electric fields(nsPEFs),characterized by short duration and high strength,significantly influence cell phenotypes and regulate MSCs differentiation via multiple pathways.Consequently,we used transcriptomics to study changes in messenger RNA(mRNA),long noncoding RNA(lncRNA),microRNA(miRNA),and circular RNA expression during nsPEFs application.AIM To explore gene expression profiles and potential transcriptional regulatory mechanisms in MSCs pretreated with nsPEFs.METHODS The impact of nsPEFs on the MSCs transcriptome was investigated through whole transcriptome sequencing.MSCs were pretreated with 5-pulse nsPEFs(100 ns at 10 kV/cm,1 Hz),followed by total RNA isolation.Each transcript was normalized by fragments per kilobase per million.Fold change and difference significance were applied to screen the differentially expressed genes(DEGs).Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to elucidate gene functions,complemented by quantitative polymerase chain reaction verification.RESULTS In total,263 DEGs were discovered,with 92 upregulated and 171 downregulated.DEGs were predominantly enriched in epithelial cell proliferation,osteoblast differentiation,mesenchymal cell differentiation,nuclear division,and wound healing.Regarding cellular components,DEGs are primarily involved in condensed chromosome,chromosomal region,actin cytoskeleton,and kinetochore.From aspect of molecular functions,DEGs are mainly involved in glycosaminoglycan binding,integrin binding,nuclear steroid receptor activity,cytoskeletal motor activity,and steroid binding.Quantitative real-time polymerase chain reaction confirmed targeted transcript regulation.CONCLUSION Our systematic investigation of the wide-ranging transcriptional pattern modulated by nsPEFs revealed the differential expression of 263 mRNAs,2 miRNAs,and 65 lncRNAs.Our study demonstrates that nsPEFs may affect stem cells through several signaling pathways,which are involved in vesicular transport,calcium ion transport,cytoskeleton,and cell differentiation.
基金US NSF Under Grant No.CMS-0202846National Natural Science Foundation of China Under Grant No.50278092
文摘This paper studies the dynamic responses of SDOF system under pulse-dominant excitations.The purpose of the study is to prepare for scrutiny of some near-field pulse-dominantground motions and their potential to cause structural damage.Extending the single pulse dynamics,we consider the effect of pulse sequences.This kind of excitation was particularly obvious in some of previous earthquakes such as Northridge (1994) and Chi-Chi (1995).Based on the duration, peak and rise and decay era of the main pulse as well as its relationship with the predecessor and successor pulses,we propose a classification for the pulse sequences.Consequent studies have been canied out for acceleration,velocity and displacement response spectra of the main pulse with either a predecessor or a successor pulse.The analysis also includes general response behaviors in different fundamental period segments and special aspects of response at certain points (e.g.,the corresponding peak points).
文摘There is a large amount of fat in the postganglionic segment of the brachial plexus nerve.The use of short T1 inversion recovery pulse sequence may improve signal strength of the brachial plexus postganglionic segment.The present study revealed that the combination of three-dimensional fast imaging employing steady-state acquisition with phase-cycled and short T1 inversion recovery pulse sequence clearly displayed the anatomical morphology and structure of the brachial plexus nerve,together with maximum intensity projection,volume rendering and other three-dimensional reconstruction techniques.Our results suggested that this method is also suitable for providing accurate assessment and diagnosis of the site,severity and scope of brachial plexus injury.
文摘A modified DBSCAN algorithm is presented for deinterleaving of radar pulses in modern EW environments.A main characteristic of the proposed method is that using only time of arrival of pulses,the method can sort the pulses efficiently.Other PDW information such as rise time,carrier frequency,pulse width,modulation on pulse,fall time and direction of arrival are not required.To identify the valid PRIs in a set of interleaved pulses,an innovative modification of the DBSCAN algorithm is introduced which is accurate and easy to implement.The proposed method determines valid PRIs more accurately and neglects the spurious ones more efficiently as compared to the classical histogram based algorithms such as SDIF.Furthermore,without specifying any input parameter,the proposed method can deinterleave radar pulses while up to 30%jitter is present in the associated PRI.The accuracy and efficiency of the proposed method are verified by computer simulations and real data results.Experimental simulations are based on different real and operational scenarios where the presence of missing and spurious pulses are also considered.So,the simulation results can be of practical significance.
基金supported by the National Natural Science Foundation of China (60774098)the National Key Basic Research Program (2006922004)
文摘On the basis of the relationship between the Hamiltonian of spin 1/2 quantum system under control and the energy level structure and transitions, a radio frequency pulse sequence is designed using intuitive and half counter-intuitive sequences of pulse to transfer the population of the 3-qubit system coherently. The effectiveness of the designed control sequence is verified through the system simulation experiment of the evolution of state. In principle, the design method of the control pulse sequence proposed can be generalized to use in the quantum systems of higher dimension.
文摘The study of phased array radar( PAR) pulse amplitude sequence characteristics is the key to understand the radar's working state and its beam's scanning manner.According to the principle of antenna pattern formation and the searching and tracking modes of beams,this paper analyzes the characteristics and differences of pulse amplitude sequence when the radar beams work in searching and tracking modes respectively.Then an optimal sorting model of pulse amplitude sequence is established based on least-squares and curve-fitting methods.This method is helpful for acquiring the current working state of the radar and recognizing its instantaneous beam pointing by sorting the pulse amplitude sequence without the necessity to estimate the antenna pattern.
基金supported by the National Natural Science Foundation of China(61801143,61971155)the National Natural Science Foundation of Heilongjiang Province(LH2020F019).
文摘Pulse repetition interval(PRI)modulation recognition and pulse sequence search are significant for effective electronic support measures.In modern electromagnetic environments,different types of inter-pulse slide radars are highly confusing.There are few available training samples in practical situations,which leads to a low recognition accuracy and poor search effect of the pulse sequence.In this paper,an approach based on bi-directional long short-term memory(BiLSTM)networks and the temporal correlation algorithm for PRI modulation recognition and sequence search under the small sample prerequisite is proposed.The simulation results demonstrate that the proposed algorithm can recognize unilinear,bilinear,sawtooth,and sinusoidal PRI modulation types with 91.43% accuracy and complete the pulse sequence search with 30% missing pulses and 50% spurious pulses under the small sample prerequisite.
文摘The group correlation properties of binary sequences is studied, and a conclusion is drawn that not only the group correlation function of a binary sequence itself is ideal, but also some of its subsets, which code length N is even, is ideal. Finally a general formula of the group correlation of a binary sequence set is derived.
文摘In this framework we present a new method for measurement of the UWB impulse train based on the parallel sampling of the cascaded identical RC filters. We show that the amplitudes and time locations of p sequential impulses can be reconstructed from simultaneous measurement of the outputs from 2p cascaded identical RC filters. The parallel sampling scheme has a wide range of applications including the detection of the ultra wideband (UWB) impulses. Due to identical analog RC filters and buffer amplifiers, the parallel sampling scheme is flexible to implement in VLSI applications.
文摘The contrast agent concentration, the time of repetition (TR) and magnetic field strength are significant parameters that influence for the accurate signal intensity (SI) in quantitative Magnetic Resonance Imaging (MRI). Therefore, this study was conducted to investigate and refine the dependence and the optimal effect of Time of Repetition (TR) on the relationship between signal intensity and Gd-DTPA (Gadolinium-diethylene-triaminepenta-acetic acid) concentration, after applying two-dimensional (2D) Spin Echo (SE) pulse sequence under low-field MRI. In addition to that, the optimal concentration of Gd-DTPA at given sequence parameters at low-field MRI was also evaluated. A water-filled phantom was constructed for a range of Gd-DTPA concentrations (0 - 6 mmol/L) and the mean signal intensities (SIs) were assessed in the defined region of interest on T1-weighted images with different TR values (40 - 2000 ms). The generated signal-concentration curves for Gd-DTPA revealed that increasing TR was associated with the increase of the overall SIs and the maximum relationship between SI to concentration. Moreover, the required Gd-DTPA concentration to produce the maximum SI was associated to decrease with the increase of TR. In addition to this, the application of beyond 100 ms TR values in this study with relatively higher concentrations (beyond 1 - 2 mmol/L) has resulted predominantly non-linear patterns in the signal-concentration curves and it appears the saturation or decay of the SIs due to T2 effect. From these results, it can be suggested that the selection of relatively lower Gd-DTPA concentration ( mmol/L) with less than 800 ms (<800 ms) TR values can produce a better linear relationship between the concertation and SIs in T1-weighted SE low field contrast-enhanced MRI. Furthermore, this study also outlined the significance and necessity of the optimization of TR in SE sequence in low field MRI prior to a particular examination.
基金supported by the National Natural Science Foundation of China(grant number:22274050)the Shanghai Science and Technology Commission(contract number:23J21900300)the Fundamental Research Funds for the Central Universities.
文摘Ribonucleic acid(RNA)structures and dynamics play a crucial role in elucidating RNA functions and facilitating the design of drugs targeting RNA and RNA-protein complexes.However,obtaining RNA structures using conventional biophysical techniques,such as Xray crystallography and solution nuclear magnetic resonance(NMR),presents challenges due to the inherent flexibility and susceptibility to degradation of RNA.In recent years,solid-state NMR(SSNMR)has rapidly emerged as a promising alternative technique for characterizing RNA structure and dynamics.SSNMR has several distinct advantages,including flexibility in sample states,the ability to capture dynamic features of RNA in solid form,and suitability to character RNAs in various sizes.Recent decade witnessed the growth of ^(1)H-detected SSNMR methods on RNA,which targeted elucidating RNA topology and base pair dynamics in solid state.They have been applied to determine the topology of RNA segment in human immunodeficiency virus(HIV)genome and the base pair dynamics of riboswitch RNA.These advancements have expanded the utility of SSNMR techniques within the RNA research field.This review provides a comprehensive discussion of recent progress in ^(1)H-detected SSNMR investigations into RNA structure and dynamics.We focus on the established ^(1)H-detected SSNMR methods,sample preparation protocols,and the implementation of rapid data acquisition approaches.
文摘本文主要探讨了大功率三电平应用中3.3 kV SiC MOSFET和IGBT的混合应用问题,针对外管高频、内管低频的调制策略中不同箝位管脉冲时序对三电平有源中点箝位(ANPC)拓扑换流路径、开关损耗以及开关特性等关键因素的影响开展分析,实验验证了不同箝位管脉冲时序对SiC MOSFET和IGBT的动态特性和开关损耗的影响。
基金supported by the National Natural Science Foundation of China(11174314,11474301,11204345)the State Key Laboratory of Acoustics,Chinese Academy of Sciences(SKLA201502)
文摘It is an efficient method to model ship radiated noise as quasi-periodic random sound pulse sequences. Based on the model, this paper discusses the characteristics change of ship noise after through shallow-water waveguides. Theoretical analysis and numerical simulation show that random waveguides and multi-path effects can bring much additional transmission loss for the noise line spectra, demonstrating the instability of ship radiated noise line spectra to a certain extent, and providing some theoretical support for advanced studies of ship radiated noise.
