Repetitive DNA sequences form a large portion of eukaryote genomes. Using wheat ( Triticum )as a model, the classification, features and functions of repetitive DNA sequences in the Tritieeae grass tribe is reviewed a...Repetitive DNA sequences form a large portion of eukaryote genomes. Using wheat ( Triticum )as a model, the classification, features and functions of repetitive DNA sequences in the Tritieeae grass tribe is reviewed as well as the role of these sequences in genome differentiation, control and regulation of homologous chromosome synapsis and pairing. Transposable elements, as an important portion of dispersed repetitives,may play an essential role in gene mutation of the host. Dynamic models for change of copy number and sequences of the repetitive family are also presented after the models of Charlesworth et al. Application of repetitive DNA sequences in the study of evolution, chromosome fingerprinting and marker assisted gene transfer and breeding are described by taking wheat as an example.展开更多
A Wi-Fi fingerprinting localization approach has attracted increasing attention in recent years due to the ubiquity of Access Point( AP). However,typical fingerprinting localization methods fail to resist accidental e...A Wi-Fi fingerprinting localization approach has attracted increasing attention in recent years due to the ubiquity of Access Point( AP). However,typical fingerprinting localization methods fail to resist accidental environmental changes,such as AP movement. In order to address this problem,a robust fingerprinting indoor localization method is initiated. In the offline phase,three attributes of Received Signal Strength Indication( RSSI) —average,standard deviation and AP's response rate—are computed to prepare for the subsequent computation. In this way,the underlying location-relevant information can be captured comprehensively. Then in the online phase, a three-step voting scheme-based decision mechanism is demonstrated, detecting and eliminating the part of AP where the signals measured are severely distorted by AP 's movement. In the following localization step,in order to achieve accuracy and efficiency simultaneously,a novel fingerprinting localization algorithm is applied. Bhattacharyya distance is utilized to measure the RSSI distribution distance,thus realizing the optimization of MAximum Overlapping algorithm( MAO). Finally,experimental results are displayed,which demonstrate the effectiveness of our proposed methods in eliminating outliers and attaining relatively higher localization accuracy.展开更多
Prior group-based fingerprinting achieves better detection performance when colluders are from the same group. But in digital wholesale and needs to be identified retail, the middleman as well as customers especially ...Prior group-based fingerprinting achieves better detection performance when colluders are from the same group. But in digital wholesale and needs to be identified retail, the middleman as well as customers especially against group colluding attack. The group tracing is neglected in previous works. In this paper, we propose a novel multimedia group fingerprint to trace malicious middlemen along with customers. We explore the quality of the non degenerate linear shift register sequence （NDLSRS） which is convenient in group initialization and management. The theoretic analysis and simulation experiment results show that NDLSRS has good resistance against attacks from colluding groups/ members even after its volume expanded.展开更多
【目的】分析玫瑰及其近缘种之间的遗传多样性并构建指纹图谱,为玫瑰种质资源鉴定与开发利用奠定基础。【方法】在玫瑰的传统品种、杂交繁育品种和国内外引进品种中各选择1种,取其鲜嫩叶片进行转录组测序;基于测序得到的玫瑰转录组数据...【目的】分析玫瑰及其近缘种之间的遗传多样性并构建指纹图谱,为玫瑰种质资源鉴定与开发利用奠定基础。【方法】在玫瑰的传统品种、杂交繁育品种和国内外引进品种中各选择1种,取其鲜嫩叶片进行转录组测序;基于测序得到的玫瑰转录组数据,使用MISA在reads覆盖的基因组数据中查找玫瑰的SSR位点,并根据SSR位点两端的保守序列使用Primer 3.0设计引物。选取10种玫瑰的DNA作为试验材料,筛选设计、合成后的引物。以48份玫瑰及其近缘种的DNA作为试验材料,利用筛选出的峰值较好的引物进行TP-M13-SSR PCR,并对其扩增产物进行毛细管电泳检测,应用GeneMarker 2.2.0(SoftGenetics,USA)读取毛细管电泳数据并用Excel进行整理;使用POPGEN VERSION 1.32计算筛选引物的观测杂合度、期望杂合度、Nei’s遗传多样性指数、观测等位基因数、有效等位基因数、Shannon信息指数,并用CERVUS version 3.0计算多态性信息含量。利用Powermarker计算玫瑰及其近缘种各种质之间的遗传距离;采用NTSYSpc 2.10e计算每2个种质之间的遗传相似性系数,并绘制UPGMA聚类树状图。最后采用引物与基因型组合的方式构建玫瑰及其近缘种的指纹图谱。【结果】基于玫瑰样品转录组测序数据,使用MISA共检测出48796个SSR位点,分布于139712条Unigene中,碱基重复类型数量最多的为二核苷酸重复和三核苷酸重复,分别为20628和12828个。使用Primer 3.0以SSR位点两端的保守序列为依据初步设计并合成了144对引物;以10个玫瑰品种的DNA作为模板筛选引物,共筛选出峰值较好的28对引物。以48份玫瑰及其近缘种的DNA为试验材料,28对引物在48份试验材料中均能扩增出峰型良好、多态性高的DNA片段;28对引物的观测杂合度、期望杂合度、Nei’s遗传多样性指数、观测等位基因数、有效等位基因数、Shannon信息指数和多态性信息含量的平均值分别为0.4101,0.7505,0.7011,4.607个,3.5116个,1.3442和0.6526。大多数供试样品间的遗传距离为0.6000~0.8000;聚类分析结果显示,在遗传相似系数为0.571时,48份玫瑰及其近缘种被分为两类。运用核心引物法筛选出的4对核心引物可将48份试验材料全部区分开,并构建了其指纹图谱。【结论】开发并筛选出28对多态性较好的SSR引物,可用于后续玫瑰的遗传多样性分析、遗传图谱构建、遗传稳定性鉴定等方面。展开更多
The genetic fingerprints of Chinese wildrye ( Aneurolepidium chinensis (Trin.) Kitag) were constructed by Southern blot analysis of Ase Ⅰ_, Dra Ⅰ_, Eco RⅠ_, Hin dⅢ_, Nco Ⅰ_, Mob Ⅰ_, Rsa Ⅰ_, Pst Ⅰ_, Taq Ⅰ_ dig...The genetic fingerprints of Chinese wildrye ( Aneurolepidium chinensis (Trin.) Kitag) were constructed by Southern blot analysis of Ase Ⅰ_, Dra Ⅰ_, Eco RⅠ_, Hin dⅢ_, Nco Ⅰ_, Mob Ⅰ_, Rsa Ⅰ_, Pst Ⅰ_, Taq Ⅰ_ digested genomic DNA probed with synthesized oligonucleotide,(CT) 8,(GCTA) 4,(GGAT) 4 or (GACA) 4 .The difference between “Jisheng 4" and Chinese wildrye was shown by RFLP analysis of Dra Ⅰ_ or Rsa Ⅰ_ digested genomic DNAs probed with (GCTA) 4 .DNA fragments were obtained by PCR performed with genomic DNA of “Jisheng 4" as a template and (CT) 8,(GCTA) 4,(GGAT) 4 or (GACA) 4 as primer. Five size 2325,1455, 876,774 and 299 bp fragments were amplified when (GGAT) 4 was used, indicating satisfied results were obtained.展开更多
文摘Repetitive DNA sequences form a large portion of eukaryote genomes. Using wheat ( Triticum )as a model, the classification, features and functions of repetitive DNA sequences in the Tritieeae grass tribe is reviewed as well as the role of these sequences in genome differentiation, control and regulation of homologous chromosome synapsis and pairing. Transposable elements, as an important portion of dispersed repetitives,may play an essential role in gene mutation of the host. Dynamic models for change of copy number and sequences of the repetitive family are also presented after the models of Charlesworth et al. Application of repetitive DNA sequences in the study of evolution, chromosome fingerprinting and marker assisted gene transfer and breeding are described by taking wheat as an example.
基金Sponsored by the National High Technology Research and Development Program of China(Grant No.2014AA123103)
文摘A Wi-Fi fingerprinting localization approach has attracted increasing attention in recent years due to the ubiquity of Access Point( AP). However,typical fingerprinting localization methods fail to resist accidental environmental changes,such as AP movement. In order to address this problem,a robust fingerprinting indoor localization method is initiated. In the offline phase,three attributes of Received Signal Strength Indication( RSSI) —average,standard deviation and AP's response rate—are computed to prepare for the subsequent computation. In this way,the underlying location-relevant information can be captured comprehensively. Then in the online phase, a three-step voting scheme-based decision mechanism is demonstrated, detecting and eliminating the part of AP where the signals measured are severely distorted by AP 's movement. In the following localization step,in order to achieve accuracy and efficiency simultaneously,a novel fingerprinting localization algorithm is applied. Bhattacharyya distance is utilized to measure the RSSI distribution distance,thus realizing the optimization of MAximum Overlapping algorithm( MAO). Finally,experimental results are displayed,which demonstrate the effectiveness of our proposed methods in eliminating outliers and attaining relatively higher localization accuracy.
文摘Prior group-based fingerprinting achieves better detection performance when colluders are from the same group. But in digital wholesale and needs to be identified retail, the middleman as well as customers especially against group colluding attack. The group tracing is neglected in previous works. In this paper, we propose a novel multimedia group fingerprint to trace malicious middlemen along with customers. We explore the quality of the non degenerate linear shift register sequence （NDLSRS） which is convenient in group initialization and management. The theoretic analysis and simulation experiment results show that NDLSRS has good resistance against attacks from colluding groups/ members even after its volume expanded.
文摘【目的】分析玫瑰及其近缘种之间的遗传多样性并构建指纹图谱,为玫瑰种质资源鉴定与开发利用奠定基础。【方法】在玫瑰的传统品种、杂交繁育品种和国内外引进品种中各选择1种,取其鲜嫩叶片进行转录组测序;基于测序得到的玫瑰转录组数据,使用MISA在reads覆盖的基因组数据中查找玫瑰的SSR位点,并根据SSR位点两端的保守序列使用Primer 3.0设计引物。选取10种玫瑰的DNA作为试验材料,筛选设计、合成后的引物。以48份玫瑰及其近缘种的DNA作为试验材料,利用筛选出的峰值较好的引物进行TP-M13-SSR PCR,并对其扩增产物进行毛细管电泳检测,应用GeneMarker 2.2.0(SoftGenetics,USA)读取毛细管电泳数据并用Excel进行整理;使用POPGEN VERSION 1.32计算筛选引物的观测杂合度、期望杂合度、Nei’s遗传多样性指数、观测等位基因数、有效等位基因数、Shannon信息指数,并用CERVUS version 3.0计算多态性信息含量。利用Powermarker计算玫瑰及其近缘种各种质之间的遗传距离;采用NTSYSpc 2.10e计算每2个种质之间的遗传相似性系数,并绘制UPGMA聚类树状图。最后采用引物与基因型组合的方式构建玫瑰及其近缘种的指纹图谱。【结果】基于玫瑰样品转录组测序数据,使用MISA共检测出48796个SSR位点,分布于139712条Unigene中,碱基重复类型数量最多的为二核苷酸重复和三核苷酸重复,分别为20628和12828个。使用Primer 3.0以SSR位点两端的保守序列为依据初步设计并合成了144对引物;以10个玫瑰品种的DNA作为模板筛选引物,共筛选出峰值较好的28对引物。以48份玫瑰及其近缘种的DNA为试验材料,28对引物在48份试验材料中均能扩增出峰型良好、多态性高的DNA片段;28对引物的观测杂合度、期望杂合度、Nei’s遗传多样性指数、观测等位基因数、有效等位基因数、Shannon信息指数和多态性信息含量的平均值分别为0.4101,0.7505,0.7011,4.607个,3.5116个,1.3442和0.6526。大多数供试样品间的遗传距离为0.6000~0.8000;聚类分析结果显示,在遗传相似系数为0.571时,48份玫瑰及其近缘种被分为两类。运用核心引物法筛选出的4对核心引物可将48份试验材料全部区分开,并构建了其指纹图谱。【结论】开发并筛选出28对多态性较好的SSR引物,可用于后续玫瑰的遗传多样性分析、遗传图谱构建、遗传稳定性鉴定等方面。
文摘The genetic fingerprints of Chinese wildrye ( Aneurolepidium chinensis (Trin.) Kitag) were constructed by Southern blot analysis of Ase Ⅰ_, Dra Ⅰ_, Eco RⅠ_, Hin dⅢ_, Nco Ⅰ_, Mob Ⅰ_, Rsa Ⅰ_, Pst Ⅰ_, Taq Ⅰ_ digested genomic DNA probed with synthesized oligonucleotide,(CT) 8,(GCTA) 4,(GGAT) 4 or (GACA) 4 .The difference between “Jisheng 4" and Chinese wildrye was shown by RFLP analysis of Dra Ⅰ_ or Rsa Ⅰ_ digested genomic DNAs probed with (GCTA) 4 .DNA fragments were obtained by PCR performed with genomic DNA of “Jisheng 4" as a template and (CT) 8,(GCTA) 4,(GGAT) 4 or (GACA) 4 as primer. Five size 2325,1455, 876,774 and 299 bp fragments were amplified when (GGAT) 4 was used, indicating satisfied results were obtained.
