BACKGROUND Ulcerative colitis(UC)is a chronic inflammatory disease affecting the colon.The most common psychological issue in UC patients is varying degrees of depre-ssion,which affects the condition and quality of li...BACKGROUND Ulcerative colitis(UC)is a chronic inflammatory disease affecting the colon.The most common psychological issue in UC patients is varying degrees of depre-ssion,which affects the condition and quality of life of UC patients and may lead to deterioration of the patient’s condition.UC drugs combined with anti-anxiety and antidepression drugs can alleviate symptoms of both depression and UC.Brain-derived neurotrophic factor(BDNF)precursor(proBDNF)/p75 neurotrophin receptor(p75NTR)/sortilin and BDNF/tropomyosin receptor kinase B(TrkB)signalling balance is essential for maintaining brain homeostasis and preventing the development of depressive behaviours.AIM To explore the mechanism by which Wuling powder regulates the proBDNF/p75NTR/sortilin and BDNF/TrkB pathways in the treatment of UC with depre-ssion.METHODS Depression was established in C57BL/6J mice via chronic restraint stress,and the UC model was induced with dextran sodium sulfate(DSS).In the treatment stage,mesalazine(MS)was the basic treatment,Wuling powder was the experimental treatment,and fluoxetine was the positive control drug for treating depression.Changes in intestinal mucosal inflammation,behaviour,and the proBDNFp75NTR/sortilin and BDNF/TrkB pathways were evaluated.RESULTS In the depression groups,Wuling powder decreased the immobility time,increased the distance travelled in the central zone and the total distance travelled,and restored balance in the proBDNF/p75NTR/sortilin and BDNF/TrkB signalling pathways.In the DSS and chronic restraint stress+DSS groups,immobility time increased,distance travelled in the central zone and total distance travelled decreased,activity of the proBDNF/p75NTR/sortilin pathway was upregulated,and activity of the BDNF/TrkB pathway was downregulated,indicating that mice with UC often have comorbid depression.Compared with those of MS alone,Wuling powder combined with MS further decreased the colon histopathological scores and the expression levels of tumor necrosis factor-alpha and interleukin-6 mRNAs.CONCLUSION This study confirmed that Wuling powder may play an antidepressant role by regulating the balance of the proBDNF/p75NTR/sortilin and BDNF/TrkB signalling pathways and further relieve intestinal inflammation in UC.展开更多
[Objectives]This study was conducted to systematically present the research and development status of Wuling Powder over the years, and comprehensively analyze the hot spots and trends of Wuling Powder in the treatmen...[Objectives]This study was conducted to systematically present the research and development status of Wuling Powder over the years, and comprehensively analyze the hot spots and trends of Wuling Powder in the treatment of diseases, so as to provide reference for scholars in this field in the future. [Methods] China National Knowledge Network(CNKI) database was used as the literature retrieval source to search the literature related to Wuling Powder in the treatment of nephropathy, and then the data visualization analysis was carried out by using the software citespace.5.7.R2. [Results] Through accurate retrieval, a total of 204 effective literatures were included. It was found that domestic research on the treatment of nephropathy by Wuling Powder was on the rise. From the analysis of knowledge graph, it was concluded that after long-term research and development, a relatively concentrated core research group had been formed in China, which mainly focused on "Wuling Powder", "chronic kidney disease" and "diabetic nephropathy". [Conclusions] The research on the treatment of nephropathy by Wuling Powder shows a good development trend, and the research content is constantly enriched, and this research is still the current and future research hotspot.展开更多
[Objectives]To compare the clinical efficacy and safety of Wuling powder and conventional Western medicine for lowering uric acid in the treatment of hyperuricemia.[Methods]Databases such as CNKI,Wanfang Data,VIP Data...[Objectives]To compare the clinical efficacy and safety of Wuling powder and conventional Western medicine for lowering uric acid in the treatment of hyperuricemia.[Methods]Databases such as CNKI,Wanfang Data,VIP Database,PubMed,Cochrane library and Embase,were retrieved,and comparative experiments of efficacy(serum uric acid level)and safety(incidence of adverse reactions)between Wuling powder(used singly or combined with other prescriptions)and conventional Western medicine in the treatment of hyperuricemia were included,and effect size was pooled with Revman 5.3.[Results]A total of 11 articles,involving 817 patients,were included,and their research quality was evaluated to be low.In terms of overall effect,Wuling powder(used singly or combined with other prescriptions)was better than the control drug in reducing serum uric acid level,but the difference was insignificant[SMD=-0.37,95%CI(-0.90,0.16)].The heterogeneity between studies was high.The incidence of adverse reactions in the experimental group was significantly lower than that in the control group.Subgroup analysis shows that Wuling powder combined with other prescriptions was better than the control drug in reducing serum uric acid level[SMD=-0.37,95%CI(-0.90,0.16),P=0.17],and the heterogeneity was reduced.[Conclusions]Wuling powder combined with other prescriptions is better than conventional Western medicine in the treatment of hyperuricemia,and has better safety.Wuling powder alone is not as effective as conventional Western medicine.It is suggested that in clinical work,doctors may need to modify the prescription of Wuling powder.The low quality of the literature supporting this conclusion suggests that this evidence should be interpreted with caution.展开更多
[Objectives]This study was conducted to investigate the renal protective effects of Wuling Powder on mice with nephrotic syndrome(NS)based on Notch pathway.[Methods]Sixty KM mice were randomly divided into normal grou...[Objectives]This study was conducted to investigate the renal protective effects of Wuling Powder on mice with nephrotic syndrome(NS)based on Notch pathway.[Methods]Sixty KM mice were randomly divided into normal group,model group,prednisone acetate positive group,high-dose Wuling Powder group,medium-dose Wuling Power group and low-dose Wuling Power group,with 10 mice in each group.Three days after prophylactic administration,a comprehensive nephropathy model was prepared by injecting 1 mg/ml doxorubicin hydrochloride solution(7.5 mg/kg)into the tail vein.After successful modeling,prednisone acetate and Wuling SAN were given high,medium and low doses for intervention for 28 d,respectively.After that,urinary protein and creatinine contents of mice in each group were detected,and pathological damage of renal tissue was observed by HE and Masson staining.The mRNA levels of Notch1,Jagged1 and Hes1 in mouse kidney tissues were detected by RT-PCR,and the expression levels of Notch1,Jagged1 and Hes1 proteins were detected by Western blot.[Results]Wuling Powder could effectively reduce the contents of urine protein(P<0.01)and Scr(P<0.01)in NS mice,and alleviate the pathological injury of kidney.Compared with the model group,the prednisone acetate group and various Wuling Powder groups could down-regulate the expressions of Notch1,Jagged1 and Hes1 mRNA in the kidney tissue of mice(P<0.01),and the expression of Notch1 protein in the renal tissue of mice decreased(P<0.01).The contents of Hes1 in the prednisone acetate group and the high-and medium-dose Wuling Powder groups significantly decreased(P<0.05).[Conclusions]Wuling Powder could protect the kidneys in mice with NS through Notch pathway.展开更多
文摘BACKGROUND Ulcerative colitis(UC)is a chronic inflammatory disease affecting the colon.The most common psychological issue in UC patients is varying degrees of depre-ssion,which affects the condition and quality of life of UC patients and may lead to deterioration of the patient’s condition.UC drugs combined with anti-anxiety and antidepression drugs can alleviate symptoms of both depression and UC.Brain-derived neurotrophic factor(BDNF)precursor(proBDNF)/p75 neurotrophin receptor(p75NTR)/sortilin and BDNF/tropomyosin receptor kinase B(TrkB)signalling balance is essential for maintaining brain homeostasis and preventing the development of depressive behaviours.AIM To explore the mechanism by which Wuling powder regulates the proBDNF/p75NTR/sortilin and BDNF/TrkB pathways in the treatment of UC with depre-ssion.METHODS Depression was established in C57BL/6J mice via chronic restraint stress,and the UC model was induced with dextran sodium sulfate(DSS).In the treatment stage,mesalazine(MS)was the basic treatment,Wuling powder was the experimental treatment,and fluoxetine was the positive control drug for treating depression.Changes in intestinal mucosal inflammation,behaviour,and the proBDNFp75NTR/sortilin and BDNF/TrkB pathways were evaluated.RESULTS In the depression groups,Wuling powder decreased the immobility time,increased the distance travelled in the central zone and the total distance travelled,and restored balance in the proBDNF/p75NTR/sortilin and BDNF/TrkB signalling pathways.In the DSS and chronic restraint stress+DSS groups,immobility time increased,distance travelled in the central zone and total distance travelled decreased,activity of the proBDNF/p75NTR/sortilin pathway was upregulated,and activity of the BDNF/TrkB pathway was downregulated,indicating that mice with UC often have comorbid depression.Compared with those of MS alone,Wuling powder combined with MS further decreased the colon histopathological scores and the expression levels of tumor necrosis factor-alpha and interleukin-6 mRNAs.CONCLUSION This study confirmed that Wuling powder may play an antidepressant role by regulating the balance of the proBDNF/p75NTR/sortilin and BDNF/TrkB signalling pathways and further relieve intestinal inflammation in UC.
基金Supported by Open Project for First-class Discipline Construction in Guangxi(2019XK120)Natural Science Research Project of Guangxi University of Chinese Medicine (2018MS008)。
文摘[Objectives]This study was conducted to systematically present the research and development status of Wuling Powder over the years, and comprehensively analyze the hot spots and trends of Wuling Powder in the treatment of diseases, so as to provide reference for scholars in this field in the future. [Methods] China National Knowledge Network(CNKI) database was used as the literature retrieval source to search the literature related to Wuling Powder in the treatment of nephropathy, and then the data visualization analysis was carried out by using the software citespace.5.7.R2. [Results] Through accurate retrieval, a total of 204 effective literatures were included. It was found that domestic research on the treatment of nephropathy by Wuling Powder was on the rise. From the analysis of knowledge graph, it was concluded that after long-term research and development, a relatively concentrated core research group had been formed in China, which mainly focused on "Wuling Powder", "chronic kidney disease" and "diabetic nephropathy". [Conclusions] The research on the treatment of nephropathy by Wuling Powder shows a good development trend, and the research content is constantly enriched, and this research is still the current and future research hotspot.
基金Supported by China Postdoctoral Science Foundation(2014M550133)Construction of Biostatistics Course Based on Mixed Teaching Platform(JYB20180724)Product Development and Cultural Dissemination of Traditional Chinese Medicine Preparations(20200107).
文摘[Objectives]To compare the clinical efficacy and safety of Wuling powder and conventional Western medicine for lowering uric acid in the treatment of hyperuricemia.[Methods]Databases such as CNKI,Wanfang Data,VIP Database,PubMed,Cochrane library and Embase,were retrieved,and comparative experiments of efficacy(serum uric acid level)and safety(incidence of adverse reactions)between Wuling powder(used singly or combined with other prescriptions)and conventional Western medicine in the treatment of hyperuricemia were included,and effect size was pooled with Revman 5.3.[Results]A total of 11 articles,involving 817 patients,were included,and their research quality was evaluated to be low.In terms of overall effect,Wuling powder(used singly or combined with other prescriptions)was better than the control drug in reducing serum uric acid level,but the difference was insignificant[SMD=-0.37,95%CI(-0.90,0.16)].The heterogeneity between studies was high.The incidence of adverse reactions in the experimental group was significantly lower than that in the control group.Subgroup analysis shows that Wuling powder combined with other prescriptions was better than the control drug in reducing serum uric acid level[SMD=-0.37,95%CI(-0.90,0.16),P=0.17],and the heterogeneity was reduced.[Conclusions]Wuling powder combined with other prescriptions is better than conventional Western medicine in the treatment of hyperuricemia,and has better safety.Wuling powder alone is not as effective as conventional Western medicine.It is suggested that in clinical work,doctors may need to modify the prescription of Wuling powder.The low quality of the literature supporting this conclusion suggests that this evidence should be interpreted with caution.
基金Supported by Natural Science Research of the Open Project of First-class Discipline Construction in Guangxi(2019XK120)Natural Science Research Project of Guangxi University of Chinese Medicine(2018MS008)College Students’Innovation and Entrepreneurship Training Program of Guangxi Autonomous Region in 2023(S202310600102).
文摘[Objectives]This study was conducted to investigate the renal protective effects of Wuling Powder on mice with nephrotic syndrome(NS)based on Notch pathway.[Methods]Sixty KM mice were randomly divided into normal group,model group,prednisone acetate positive group,high-dose Wuling Powder group,medium-dose Wuling Power group and low-dose Wuling Power group,with 10 mice in each group.Three days after prophylactic administration,a comprehensive nephropathy model was prepared by injecting 1 mg/ml doxorubicin hydrochloride solution(7.5 mg/kg)into the tail vein.After successful modeling,prednisone acetate and Wuling SAN were given high,medium and low doses for intervention for 28 d,respectively.After that,urinary protein and creatinine contents of mice in each group were detected,and pathological damage of renal tissue was observed by HE and Masson staining.The mRNA levels of Notch1,Jagged1 and Hes1 in mouse kidney tissues were detected by RT-PCR,and the expression levels of Notch1,Jagged1 and Hes1 proteins were detected by Western blot.[Results]Wuling Powder could effectively reduce the contents of urine protein(P<0.01)and Scr(P<0.01)in NS mice,and alleviate the pathological injury of kidney.Compared with the model group,the prednisone acetate group and various Wuling Powder groups could down-regulate the expressions of Notch1,Jagged1 and Hes1 mRNA in the kidney tissue of mice(P<0.01),and the expression of Notch1 protein in the renal tissue of mice decreased(P<0.01).The contents of Hes1 in the prednisone acetate group and the high-and medium-dose Wuling Powder groups significantly decreased(P<0.05).[Conclusions]Wuling Powder could protect the kidneys in mice with NS through Notch pathway.
