The X-ray fluorescence(XRF)core scanning method is widely applied in studies of sedimentary paleoenvironments due to its convenient pretreatment,nondestructive characteristics,fast execution,continuous scanning,and hi...The X-ray fluorescence(XRF)core scanning method is widely applied in studies of sedimentary paleoenvironments due to its convenient pretreatment,nondestructive characteristics,fast execution,continuous scanning,and high resolution.XRF core scanning for sediments is commonly used in the studies on the South China Sea.This study compares XRF-scanned intensities and measured inductively coupled plasma(ICP)elemental contents of core CS11 in the northeast South China Sea deep basin.The results show that the analyzed elements can be separated into three classes.Class I includes elements with high correlation coefficients,such as Ca,Sr,and Zr;Class II contains elements with average correlation coefficients,such as Fe,Mn,Ti,and Cu;and Class III comprises elements with low correlation coefficients,such as K,Ni,Zn,Rb,and Al.In the South China Sea deep basin,pore water,compaction,and grain size have weak effects on the elemental intensities and contents of short core sediments.Hence,for elements with high correlation coefficients,a linear relationship model can be established by the least-squares method,in which the converted XRF intensities are approximately equal to the measured ICP contents.Based on the established log-ratio calibration model,the resulting ln(K/Ca),ln(Ti/Ca),ln(Fe/Ca),and ln(Zr/Ca)values generally display the same variation trends as the measured curves.The elemental contents and ratios produced by the linear model via the least-squares method and the log-ratio calibration model are expected to provide high-resolution data support for future paleoenvironmental research on the South China Sea deep basin.展开更多
Analyzed and calculated are pressure changes and body deformation of the sample inside of the corer in the process of sampling of deep-sea shallow sediment with a non-piston corer for gas hydrate investigation, Two co...Analyzed and calculated are pressure changes and body deformation of the sample inside of the corer in the process of sampling of deep-sea shallow sediment with a non-piston corer for gas hydrate investigation, Two conclusions are drawn： （1） the stress increments associated with the corer through the sampling process do not affect the stabilization of the gas hydrate; （2） the body deformation of the sample is serious and the ＂incremental filling ratio＂ （IFR） is less than unit, For taking samples with in-situ pressure and structure, combining with the design theories of the pressure tight corer, we have designed a kind of piston corer, named the gas hydrate pressure tight piston corer, Several tests on the sea have been conducted. Test results indicate that the piston corer has a good ability of taking sediment samples on the seafloor and maintaining their original in-situ pressure, meeting the requirement of exploration of gas hydrate in deep-sea shallow sediment layers.展开更多
The sinkage of a moving tracked mining vehicle is greatly af fected by the combined compression-shear rheological properties of soft deep-sea sediments. For test purposes, the best sediment simulant is prepared based ...The sinkage of a moving tracked mining vehicle is greatly af fected by the combined compression-shear rheological properties of soft deep-sea sediments. For test purposes, the best sediment simulant is prepared based on soft deep-sea sediment from a C-C poly-metallic nodule mining area in the Pacific Ocean. Compressive creep tests and shear creep tests are combined to obtain compressive and shear rheological parameters to establish a combined compressive-shear rheological constitutive model and a compression-sinkage rheological constitutive model. The combined compression-shear rheological sinkage of the tracked mining vehicle at dif ferent speeds is calculated using the Recur Dyn software with a selfprogrammed subroutine to implement the combined compression-shear rheological constitutive model. The model results are compared with shear rheological sinkage and ordinary sinkage(without consideration of rheological properties). These results show that the combined compression-shear rheological constitutive model must be taken into account when calculating the sinkage of a tracked mining vehicle. The combined compression-shear rheological sinkage decrease with vehicle speed and is the largest among the three types of sinkage. The developed subroutine in the Recur Dyn software can be used to study the performance and structural optimization of moving tracked mining vehicles.展开更多
Tellurium is a sort of scattered rare element on the earth. Its concentration is very low in earth＇s crust, only 1.0 ng/g. However, it has extremely high abundance in Co-rich crusts, marine polymetallic nodules, deep...Tellurium is a sort of scattered rare element on the earth. Its concentration is very low in earth＇s crust, only 1.0 ng/g. However, it has extremely high abundance in Co-rich crusts, marine polymetallic nodules, deep-sea sediments and aerolites. To find out the origin of tellurium enrichment in deep-sea sediments, we analyzed and compared tellurium concentrations and helium isotope compositions in the magnetic parts and those in the bulk parts of deep-sea sediments. The result indicates that the helium content, 3He/4He ratio and tellurium concentration are obviously higher in the magnetic parts than those in the bulk parts. The 3He abundance varies synchronously with the tellurium concentration. 3He and Te have a distinct positive correlation with each other. It is the first time that the paper brings forward that the extreme enrichment of tellurium in deep-sea sediments, like helium isotope anomalies, probably results from the input of interplanetary dust particles （IDPs）. Similarly, the extreme enrichment of tellurium in marine polymetallic nodules and Co-rich crusts is possibly related to IDPs.展开更多
A depth profile of bacterial community structure in one deep-sea sediment core of the western Pacific ＂warm pool＂ （WP） was investigated and compared with that in a sediment sample from the eastern Pacific （EP） b...A depth profile of bacterial community structure in one deep-sea sediment core of the western Pacific ＂warm pool＂ （WP） was investigated and compared with that in a sediment sample from the eastern Pacific （EP） by phylogenetic analysis of 16S rDNA fragments. Five bacterial 16S rDNA clone libraries were constructed, and 133 clones with different restriction fragment length polymorphism （RFLP） patterns were sequenced. A phylogenetic analysis of these sequences revealed that the bacterial diversity in a sample from the WP was more abundant than that in the EP sample. The bacterial population in the sediment core of WP was composed of eight major lineages of the domain bacteria. Among them the γ-Proteobacteria was the predominant and most diverse group in each section of WP sediment core, followed by the α-Proteobacteria. The genus Colwellia belonging to γ-Proteobacteria was predominant in this sample. The shift of bacterial communities among different sections of the WP sediment core was δ-, ε-Proteobacteria, and Cytopahga-Flexibacteria-Bacteroides （CFB） group. The ratios between them in the bacterial communities all showed inversely proportional to the depth of sediment. The sequences related to sulphate reducing bacteria （SRB） were detected in every section. The bacterial community structure in this sediment core might be related to the environmental characteristics of the surface seawater of the western Pacific WP.展开更多
The traction characteristics of the grouser, cutting the simulative soil of deepsea sediment, with different tooth widths, tooth heights, and ground pressures are studied with traction characteristic test apparatus. A...The traction characteristics of the grouser, cutting the simulative soil of deepsea sediment, with different tooth widths, tooth heights, and ground pressures are studied with traction characteristic test apparatus. A traction-displacement model is obtained by combining the analysis of the cutting mechanism. The results show that the tractiondisplacement curves of grousers with different tooth widths, tooth heights, and ground pressures have the same changing trend, which matches the Wong traction model. Their sensitivity coefficient and shear modulus are slightly fluctuated. Therefore, the average values can be used as the traction model parameters. The maximum traction of the grouser with a two-side edge and a 10 mm tooth width increment changing with the tooth height and ground pressure can be determined according to the grousers with different tooth widths. By combining the traction model parameters, the traction-displacement curve of the grouser with a certain group values of tooth width, tooth height, and ground pressure can be predicted. Therefore, the slip of the mining machine can be prevented to improve the mining efficiency.展开更多
Microbial community structures in the Arctic deep-sea sedimentary ecosystem are determined by organic matter input, energy availability, and other environmental factors. However, global warming and earlier ice-cover m...Microbial community structures in the Arctic deep-sea sedimentary ecosystem are determined by organic matter input, energy availability, and other environmental factors. However, global warming and earlier ice-cover melting are affecting the microbial diversity. To characterize the Arctic deep-sea sediment microbial diversity and its relationship with environmental factors, we applied Roche 454 sequencing of 16 S r DNA amplicons from Arctic deep-sea sediment sample. Both bacterial and archaeal communities＇ richness, compositions and structures as well as taxonomic and phylogenetic affiliations of identified clades were characterized. Phylotypes relating to sulfur reduction and chemoorganotrophic lifestyle are major groups in the bacterial groups; while the archaeal community is dominated by phylotypes most closely related to the ammonia-oxidizing Thaumarchaeota（96.66%） and methanogenic Euryarchaeota（3.21%）. This study describes the microbial diversity in the Arctic deep marine sediment（〉3 500 m） near the North Pole and would lay foundation for future functional analysis on microbial metabolic processes and pathways predictions in similar environments.展开更多
A deep-sea bacterium from the Pacific Ocean identified as Tenacibaculum sp. HMG1 was found to have strong malachite green（MG） degradation activity. The MG tolerance and decolorizing activities of strain HMG1 were co...A deep-sea bacterium from the Pacific Ocean identified as Tenacibaculum sp. HMG1 was found to have strong malachite green（MG） degradation activity. The MG tolerance and decolorizing activities of strain HMG1 were confirmed by bacterial growth and high-performance liquid chromatography（HPLC） analyses. Strain HMG1 was capable of removing 98.8% of the MG in cultures within 12 h and was able to grow vigorously at 20 mg/L MG. A peroxidase gene detected in the genome of strain HMG1 was found to be involved in the MG biodegradation process. The corresponding recombinant peroxidase（r POD） demonstrated high degradative activity at 1 000 mg/L MG. Based on the common candidate intermediates, strain HMG1 was inferred to have one primary MG degradation pathway containing r POD. In addition, five other candidate intermediates of the r POD-MG degradative process were detected. The optimal conditions for MG degradation were determined and showed that strain HMG1 and the r POD enzyme could maintain high bioactivity at a low temperature（20℃）, variable p H values（6.0–9.0）, higher salinities（100 mmol/L） and other factors, such as multiple metal ions, H2O2 and EDTA.MG-tolerant strain Tenacibaculum sp. HMG1 and its peroxidase have prospective applications as environmental amendments for MG degradation during coastal remediation.展开更多
A protocol to coextract the microbial metagenomic DNA and RNA from deep-sea sediment was developed for the microbiological study of environmental samples. The obtained pure metagenomic DNA with the size larger than 23...A protocol to coextract the microbial metagenomic DNA and RNA from deep-sea sediment was developed for the microbiological study of environmental samples. The obtained pure metagenomic DNA with the size larger than 23 kb and stable RNA could be used directly for PCR and reverse transcription - PCR （ RT - PCR） respectively. The direct lysis including the treatments of SDS, proteinase and lysozyme was applied to acquiring the metagenomic DNA and RNA furthest. Prior to the lysis treatment, the glass bead and denaturing solution were added to enhance the lysis efficiency and keep the integrity of RNA respectively. Denaturing gradient gel electrophoresis （DGGE） was applied in accessing the microbial 16S rRNA diversity by PCR and RT -PCR amplification from a single extraction. The pattern obtained by this analysis revealed some differences between them, indicating the efficiency of the protocol in extracting the metagenomic DNA and total RNA from deep-sea sediment.展开更多
Hydrothermal materials in deep-sea sediments provide a robust tracer to the localized hydrothermal activity at mid-ocean ridges. Major, trace and rare earth element（REE） data for surface sediments collected from the...Hydrothermal materials in deep-sea sediments provide a robust tracer to the localized hydrothermal activity at mid-ocean ridges. Major, trace and rare earth element（REE） data for surface sediments collected from the ultraslow spreading Southwest Indian Ridge are presented to examine the existence of hydrothermal component.Biogenic carbonate oozes dominate all the sediment samples, with CaO content varying from 85.5% to 89.9% on a volatile-free basis. The leaching residue of bulk sediments by ~5% HCl is compositionally comparable to the Upper Continental Crust（UCC） in SiO_2, Al_2O_3, CaO, MgO, alkali elements（Rb, Cs） and high field strength elements（Nb, Ta, Zr, Hf, Ti）. These detritus-hosted elements are inferred to be prominently derived from the Australian continent by means of eolian dust, while the contribution of local volcaniclastics is insignificant. In addition, the residual fraction shows a clear enrichment in Fe, Mn, and Ba compared with the UCC. Combining the positive Eu anomaly of residual fraction which is opposed to the UCC but the characteristic of hydrothermal fluids and associated precipitates occurred at mid-ocean ridges, the incorporation of localized hydrothermal component can be constrained. REE mixing calculations indicate that more than half REE within the residual fraction（~55%–60%） are derived from a hydrothermal component, which is inferred to be resulted from a diffuse fluid mineralization. The low-temperature diffuse flow may be widely distributed along the slow-ultraslow spreading ridges where crustal faults and fissures abound, and probably have a great mineralization potential.展开更多
Diversity of bacteria was studied in deep-sea sediments from the Shikoku Basin in the Northwest Pacific Ocean by PCR, RFLP and sequence analysis of 16S rDNA and comparing with Genbank database. Based on the RFLP profi...Diversity of bacteria was studied in deep-sea sediments from the Shikoku Basin in the Northwest Pacific Ocean by PCR, RFLP and sequence analysis of 16S rDNA and comparing with Genbank database. Based on the RFLP profile generated, 77 clones from the 16S rDNA library were divided into 27 types. Phylogenetic analysis showed that the 27 independent clones fell into four groups: Proteobac-teria (62.96%), Chloroflexi (14.81%), Planctomycetes (14.81%) and Acidobacteria (7.41%). Among all sequenced clones, 6 were related to the sulfur or sulfate metabolism bacteria and the results also demonstrated that some bacteria in deep-sea sediments had relation to matter-energy circulation.展开更多
Zenisu deep-sea channel originated from a volcanic arc region, Izu-Ogasawara Island Arc, and vanished in the Shikoku Basin of the Philippine Sea. According to the swath bathymetry, the deep-sea channel can be divided ...Zenisu deep-sea channel originated from a volcanic arc region, Izu-Ogasawara Island Arc, and vanished in the Shikoku Basin of the Philippine Sea. According to the swath bathymetry, the deep-sea channel can be divided into three segments. They are Zenisu canyon, E-W fan channel and trough-axis channel. A lot of volcanic detritus were deposited in the Zenisu Trough via the deep-sea channel because it originated from volcanic arc settings. On the basis of the swath bathymetry, submersible and seismic reflection data, the deposits are characterized by turbidite and debrite deposits as those in the other major deep-sea channels. Erosion or few sediments were observed in the Zenisu canyon,whereas a lot of turbidites and debrites occurred in the E-W channel and trough axis channel. Cold seep communities,active fault and fluid flow were discovered along the lower slope of the Zenisu Ridge. Vertical sedimentary sequences in the Zenisu Trough consist of the four post-rift sequence units of the Shikoku Basin, among which Units A and B are two turbidite units. The development of Zenisu canyon is controlled by the N-S shear fault, the E-W fan channel is related to the E-W shear fault, and the trough-axis channel is related to the subsidence of central basin.展开更多
Manganese nodules are deposit rich in manganese and other metals which "grow" on the ocean floor. The study of manganese nodule growth rate and deep sea sediment has both scientific and economic significance...Manganese nodules are deposit rich in manganese and other metals which "grow" on the ocean floor. The study of manganese nodule growth rate and deep sea sediment has both scientific and economic significance. Natural <sup>10</sup>Be （half-life= 1.5 Ma ）is produced from spallations of atmospheric nitrogen and oxygen induced by cosmic ray in the stratosphere. After several years of residence in the atmosphere, the <sup>10</sup>Be falls on the earth or deposits in the ocean, accumulating in marine展开更多
The response of bacteria to various carbohydrates in the deep-sea sediments and the Antarctic soils was investigated using cellulose, chitin, and olive oil. It was found that the carbohydrates significantly increased ...The response of bacteria to various carbohydrates in the deep-sea sediments and the Antarctic soils was investigated using cellulose, chitin, and olive oil. It was found that the carbohydrates significantly increased the corresponding specific ectoenzyme activity （β- glucosidase, β-N-acetylglucosaminidase, lipase） in the samples from deep-sea sediments. In the case of Antarctic soil samples, the cellulose or olive oil amendments had minor or no effect on β-glucosidase or lipase activity, except the chitin which stimulated β- N-acetylglucosaminidase production. The responses of the bacteria in the deep-sea sediment sample WP02-3 and the Antarctic soil sample CC-TY2 towards the chitin amendment were further analyzed. Chitin amendments were shown to stimulate the ectoenzyme activity in all the tested sediments and the soils. The bacterial response before and after the carbohydrates amendments were compared by denaturing gradient gel electrophoresis and quantitative competitive polymerase chain reaction. Significant changes were found in the structure and density of the bacterial community in the deep sea sediments as compared to the Antarctic soil sample, where the effects were relatively lower. There was no change in the bacterial population in both studied samples in response to carbohydrates amendments. These data indicate that the bacterial communities in the oligotrophic deep-sea sediments are more dynamic than that in the Antarctic soils as they respond to the nutrient sources efficiently by regulation of ectoenzyme activity and/or changing community structure.展开更多
以Web of Science引文数据库为检索对象,对1900-2012年间全球发表的深海资源相关论文进行文献计量学分析。从年度发文量与被引用量、主要论文著者、主要发文国家与机构等角度揭示深海资源研究发展轨迹。通过高频次关键词分析得出深海资...以Web of Science引文数据库为检索对象,对1900-2012年间全球发表的深海资源相关论文进行文献计量学分析。从年度发文量与被引用量、主要论文著者、主要发文国家与机构等角度揭示深海资源研究发展轨迹。通过高频次关键词分析得出深海资源研究的5大问题是:生物物种、进化及多样性;沉积物;大洋环流与气候;有机物;生态环境、群落结构。从高被引与前沿热点分析得出深海沉积物与深海生物资源是其热点研究问题,且依然是未来深海资源研究的主要学科问题。展开更多
A pressure tight sediment sampling technology, which can be introduced into the modification of the piston corer to accommodate the investigation of gas hydrate, is put forward. In this paper, the three basic rules of...A pressure tight sediment sampling technology, which can be introduced into the modification of the piston corer to accommodate the investigation of gas hydrate, is put forward. In this paper, the three basic rules of the technology are analyzed in detail: specimen transferring rule, seal self-tightening rule and semi-active pressure holding rule. Based on these, the structure of the pressure tight piston corer is put forward and its working principle is analyzed. Finally, a pressure tight sediment sampler, to which the same technology is applied, is researched through experiments. Results show that the sampler based on the above-mentioned theory has a good ability in sampling and in -situ pressure holding.展开更多
High resolution elemental records in core MD05-2903 from the northern South China Sea were obtained from XRF scanning and grain size and mineral component analyses.The K/Ti curve reveals climate changes since MIS 3 on...High resolution elemental records in core MD05-2903 from the northern South China Sea were obtained from XRF scanning and grain size and mineral component analyses.The K/Ti curve reveals climate changes since MIS 3 on a millennial scale,representing one of the best such records so far from deep-sea sediments of the South China Sea.When compared our K/Ti record with other climate records from high latitude ice cores and from East Asia stalagmites,variations in the K/Ti record show some typical saw-shaped features of rapid climate changes in the Northern Hemisphere.Grain size analysis reveals a close relationship between variations in K/Ti and in the fine end-member grain size.XRD analysis confirms that high K/Ti layers were dominated by K-rich weathering minerals,especially illite,while low K/Ti layers contain low illite but high abundance of Ti-rich heavy minerals.Therefore,the K/Ti record reflects changes in the input and deposition of fine weathering minerals and heavy minerals in the northern South China Sea region during stadials and interstadials.These elemental changes were largely controlled by variations in rainfall,erosion,and fluvial transportation induced by East Asia Monsoon.展开更多
The South China Sea is the westernmost marginal sea in the west Pacific Ocean. On thebasis of the analyses of sedimentary components, biofossils, oxygen-carbon isotopes and theirdatings in four seafloor cores which we...The South China Sea is the westernmost marginal sea in the west Pacific Ocean. On thebasis of the analyses of sedimentary components, biofossils, oxygen-carbon isotopes and theirdatings in four seafloor cores which were collected from the deep sea plain to continentalslope in the northern South China Sea, the environmental variations were basically similarto other tropical ocean areas, and their cycle changes in about the ages of 40 ka and 20 kawere more apparent since the late Pleistocene. During the warm climates, the depositionrate on the continental slope was about 2.6 times as rapid as on the deep sea plain margin.The seafloor in the deep sea plain was below the CaCO_3 compensation depth. During thecold periods, however, the deposition rate on the deep sea plain margin was slightly quickerthan on the continental slope due to the sea level reduction on a larger scale and moresupply of the terrigenous clastics.展开更多
基金This study was jointly supported by the National Natural Science Foundation of China(Nos.41576058 and 41976192)the Project of China Geological Survey(No.DD20191010)+2 种基金the Shandong Provincial Natural Science Foundation of China(No.ZR2020MD061)the Open Foundation of the State Key Laboratory of Loess and Quaternary Geology(Nos.SKLLQG1707 and SKLLQG1805)the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDB40000000).
文摘The X-ray fluorescence(XRF)core scanning method is widely applied in studies of sedimentary paleoenvironments due to its convenient pretreatment,nondestructive characteristics,fast execution,continuous scanning,and high resolution.XRF core scanning for sediments is commonly used in the studies on the South China Sea.This study compares XRF-scanned intensities and measured inductively coupled plasma(ICP)elemental contents of core CS11 in the northeast South China Sea deep basin.The results show that the analyzed elements can be separated into three classes.Class I includes elements with high correlation coefficients,such as Ca,Sr,and Zr;Class II contains elements with average correlation coefficients,such as Fe,Mn,Ti,and Cu;and Class III comprises elements with low correlation coefficients,such as K,Ni,Zn,Rb,and Al.In the South China Sea deep basin,pore water,compaction,and grain size have weak effects on the elemental intensities and contents of short core sediments.Hence,for elements with high correlation coefficients,a linear relationship model can be established by the least-squares method,in which the converted XRF intensities are approximately equal to the measured ICP contents.Based on the established log-ratio calibration model,the resulting ln(K/Ca),ln(Ti/Ca),ln(Fe/Ca),and ln(Zr/Ca)values generally display the same variation trends as the measured curves.The elemental contents and ratios produced by the linear model via the least-squares method and the log-ratio calibration model are expected to provide high-resolution data support for future paleoenvironmental research on the South China Sea deep basin.
基金The project was financially supported bythe National Natural science Foundation of China (Grant No.50675055)
文摘Analyzed and calculated are pressure changes and body deformation of the sample inside of the corer in the process of sampling of deep-sea shallow sediment with a non-piston corer for gas hydrate investigation, Two conclusions are drawn： （1） the stress increments associated with the corer through the sampling process do not affect the stabilization of the gas hydrate; （2） the body deformation of the sample is serious and the ＂incremental filling ratio＂ （IFR） is less than unit, For taking samples with in-situ pressure and structure, combining with the design theories of the pressure tight corer, we have designed a kind of piston corer, named the gas hydrate pressure tight piston corer, Several tests on the sea have been conducted. Test results indicate that the piston corer has a good ability of taking sediment samples on the seafloor and maintaining their original in-situ pressure, meeting the requirement of exploration of gas hydrate in deep-sea shallow sediment layers.
基金Supported by the National Natural Science Foundation of China(Nos.51274251,11502226)
文摘The sinkage of a moving tracked mining vehicle is greatly af fected by the combined compression-shear rheological properties of soft deep-sea sediments. For test purposes, the best sediment simulant is prepared based on soft deep-sea sediment from a C-C poly-metallic nodule mining area in the Pacific Ocean. Compressive creep tests and shear creep tests are combined to obtain compressive and shear rheological parameters to establish a combined compressive-shear rheological constitutive model and a compression-sinkage rheological constitutive model. The combined compression-shear rheological sinkage of the tracked mining vehicle at dif ferent speeds is calculated using the Recur Dyn software with a selfprogrammed subroutine to implement the combined compression-shear rheological constitutive model. The model results are compared with shear rheological sinkage and ordinary sinkage(without consideration of rheological properties). These results show that the combined compression-shear rheological constitutive model must be taken into account when calculating the sinkage of a tracked mining vehicle. The combined compression-shear rheological sinkage decrease with vehicle speed and is the largest among the three types of sinkage. The developed subroutine in the Recur Dyn software can be used to study the performance and structural optimization of moving tracked mining vehicles.
基金This work was supported by the National Natural Science Foundation of China(No.49873002)Key Basic Research Project of Ministry of Land and Mineral Resources(No.20010209).
文摘Tellurium is a sort of scattered rare element on the earth. Its concentration is very low in earth＇s crust, only 1.0 ng/g. However, it has extremely high abundance in Co-rich crusts, marine polymetallic nodules, deep-sea sediments and aerolites. To find out the origin of tellurium enrichment in deep-sea sediments, we analyzed and compared tellurium concentrations and helium isotope compositions in the magnetic parts and those in the bulk parts of deep-sea sediments. The result indicates that the helium content, 3He/4He ratio and tellurium concentration are obviously higher in the magnetic parts than those in the bulk parts. The 3He abundance varies synchronously with the tellurium concentration. 3He and Te have a distinct positive correlation with each other. It is the first time that the paper brings forward that the extreme enrichment of tellurium in deep-sea sediments, like helium isotope anomalies, probably results from the input of interplanetary dust particles （IDPs）. Similarly, the extreme enrichment of tellurium in marine polymetallic nodules and Co-rich crusts is possibly related to IDPs.
文摘A depth profile of bacterial community structure in one deep-sea sediment core of the western Pacific ＂warm pool＂ （WP） was investigated and compared with that in a sediment sample from the eastern Pacific （EP） by phylogenetic analysis of 16S rDNA fragments. Five bacterial 16S rDNA clone libraries were constructed, and 133 clones with different restriction fragment length polymorphism （RFLP） patterns were sequenced. A phylogenetic analysis of these sequences revealed that the bacterial diversity in a sample from the WP was more abundant than that in the EP sample. The bacterial population in the sediment core of WP was composed of eight major lineages of the domain bacteria. Among them the γ-Proteobacteria was the predominant and most diverse group in each section of WP sediment core, followed by the α-Proteobacteria. The genus Colwellia belonging to γ-Proteobacteria was predominant in this sample. The shift of bacterial communities among different sections of the WP sediment core was δ-, ε-Proteobacteria, and Cytopahga-Flexibacteria-Bacteroides （CFB） group. The ratios between them in the bacterial communities all showed inversely proportional to the depth of sediment. The sequences related to sulphate reducing bacteria （SRB） were detected in every section. The bacterial community structure in this sediment core might be related to the environmental characteristics of the surface seawater of the western Pacific WP.
基金Project supported by the National Natural Science Foundation of China(No.51274251)
文摘The traction characteristics of the grouser, cutting the simulative soil of deepsea sediment, with different tooth widths, tooth heights, and ground pressures are studied with traction characteristic test apparatus. A traction-displacement model is obtained by combining the analysis of the cutting mechanism. The results show that the tractiondisplacement curves of grousers with different tooth widths, tooth heights, and ground pressures have the same changing trend, which matches the Wong traction model. Their sensitivity coefficient and shear modulus are slightly fluctuated. Therefore, the average values can be used as the traction model parameters. The maximum traction of the grouser with a two-side edge and a 10 mm tooth width increment changing with the tooth height and ground pressure can be determined according to the grousers with different tooth widths. By combining the traction model parameters, the traction-displacement curve of the grouser with a certain group values of tooth width, tooth height, and ground pressure can be predicted. Therefore, the slip of the mining machine can be prevented to improve the mining efficiency.
基金The National Natural Science Foundation of China under contract No.41121064the NSFC-Shandong Joint Fund for Marine Science Research Centers under contract No.U1406403the Science Foundation for Post Doctorate Research from the Chinese Academy of Sciences under contract No.2012M511072
文摘Microbial community structures in the Arctic deep-sea sedimentary ecosystem are determined by organic matter input, energy availability, and other environmental factors. However, global warming and earlier ice-cover melting are affecting the microbial diversity. To characterize the Arctic deep-sea sediment microbial diversity and its relationship with environmental factors, we applied Roche 454 sequencing of 16 S r DNA amplicons from Arctic deep-sea sediment sample. Both bacterial and archaeal communities＇ richness, compositions and structures as well as taxonomic and phylogenetic affiliations of identified clades were characterized. Phylotypes relating to sulfur reduction and chemoorganotrophic lifestyle are major groups in the bacterial groups; while the archaeal community is dominated by phylotypes most closely related to the ammonia-oxidizing Thaumarchaeota（96.66%） and methanogenic Euryarchaeota（3.21%）. This study describes the microbial diversity in the Arctic deep marine sediment（〉3 500 m） near the North Pole and would lay foundation for future functional analysis on microbial metabolic processes and pathways predictions in similar environments.
基金The Scientific Research Project of Xiamen Southern Oceanographic Center under contract No.17GZP007NF03the China Ocean Mineral Resources R&D Association under contract No.DY-125-22-QY-18
文摘A deep-sea bacterium from the Pacific Ocean identified as Tenacibaculum sp. HMG1 was found to have strong malachite green（MG） degradation activity. The MG tolerance and decolorizing activities of strain HMG1 were confirmed by bacterial growth and high-performance liquid chromatography（HPLC） analyses. Strain HMG1 was capable of removing 98.8% of the MG in cultures within 12 h and was able to grow vigorously at 20 mg/L MG. A peroxidase gene detected in the genome of strain HMG1 was found to be involved in the MG biodegradation process. The corresponding recombinant peroxidase（r POD） demonstrated high degradative activity at 1 000 mg/L MG. Based on the common candidate intermediates, strain HMG1 was inferred to have one primary MG degradation pathway containing r POD. In addition, five other candidate intermediates of the r POD-MG degradative process were detected. The optimal conditions for MG degradation were determined and showed that strain HMG1 and the r POD enzyme could maintain high bioactivity at a low temperature（20℃）, variable p H values（6.0–9.0）, higher salinities（100 mmol/L） and other factors, such as multiple metal ions, H2O2 and EDTA.MG-tolerant strain Tenacibaculum sp. HMG1 and its peroxidase have prospective applications as environmental amendments for MG degradation during coastal remediation.
文摘A protocol to coextract the microbial metagenomic DNA and RNA from deep-sea sediment was developed for the microbiological study of environmental samples. The obtained pure metagenomic DNA with the size larger than 23 kb and stable RNA could be used directly for PCR and reverse transcription - PCR （ RT - PCR） respectively. The direct lysis including the treatments of SDS, proteinase and lysozyme was applied to acquiring the metagenomic DNA and RNA furthest. Prior to the lysis treatment, the glass bead and denaturing solution were added to enhance the lysis efficiency and keep the integrity of RNA respectively. Denaturing gradient gel electrophoresis （DGGE） was applied in accessing the microbial 16S rRNA diversity by PCR and RT -PCR amplification from a single extraction. The pattern obtained by this analysis revealed some differences between them, indicating the efficiency of the protocol in extracting the metagenomic DNA and total RNA from deep-sea sediment.
基金The National Key Basic Research Program of China under contract Nos 2013CB429705 and 2013CB429701the National Natural Science Foundation of China under contract Nos 41176045 and 41376067+1 种基金the Scientific Research Fund of the Second Institute of Oceanographythe SOA of China under contract Nos JG1403 and JT1304
文摘Hydrothermal materials in deep-sea sediments provide a robust tracer to the localized hydrothermal activity at mid-ocean ridges. Major, trace and rare earth element（REE） data for surface sediments collected from the ultraslow spreading Southwest Indian Ridge are presented to examine the existence of hydrothermal component.Biogenic carbonate oozes dominate all the sediment samples, with CaO content varying from 85.5% to 89.9% on a volatile-free basis. The leaching residue of bulk sediments by ~5% HCl is compositionally comparable to the Upper Continental Crust（UCC） in SiO_2, Al_2O_3, CaO, MgO, alkali elements（Rb, Cs） and high field strength elements（Nb, Ta, Zr, Hf, Ti）. These detritus-hosted elements are inferred to be prominently derived from the Australian continent by means of eolian dust, while the contribution of local volcaniclastics is insignificant. In addition, the residual fraction shows a clear enrichment in Fe, Mn, and Ba compared with the UCC. Combining the positive Eu anomaly of residual fraction which is opposed to the UCC but the characteristic of hydrothermal fluids and associated precipitates occurred at mid-ocean ridges, the incorporation of localized hydrothermal component can be constrained. REE mixing calculations indicate that more than half REE within the residual fraction（~55%–60%） are derived from a hydrothermal component, which is inferred to be resulted from a diffuse fluid mineralization. The low-temperature diffuse flow may be widely distributed along the slow-ultraslow spreading ridges where crustal faults and fissures abound, and probably have a great mineralization potential.
文摘Diversity of bacteria was studied in deep-sea sediments from the Shikoku Basin in the Northwest Pacific Ocean by PCR, RFLP and sequence analysis of 16S rDNA and comparing with Genbank database. Based on the RFLP profile generated, 77 clones from the 16S rDNA library were divided into 27 types. Phylogenetic analysis showed that the 27 independent clones fell into four groups: Proteobac-teria (62.96%), Chloroflexi (14.81%), Planctomycetes (14.81%) and Acidobacteria (7.41%). Among all sequenced clones, 6 were related to the sulfur or sulfate metabolism bacteria and the results also demonstrated that some bacteria in deep-sea sediments had relation to matter-energy circulation.
文摘Zenisu deep-sea channel originated from a volcanic arc region, Izu-Ogasawara Island Arc, and vanished in the Shikoku Basin of the Philippine Sea. According to the swath bathymetry, the deep-sea channel can be divided into three segments. They are Zenisu canyon, E-W fan channel and trough-axis channel. A lot of volcanic detritus were deposited in the Zenisu Trough via the deep-sea channel because it originated from volcanic arc settings. On the basis of the swath bathymetry, submersible and seismic reflection data, the deposits are characterized by turbidite and debrite deposits as those in the other major deep-sea channels. Erosion or few sediments were observed in the Zenisu canyon,whereas a lot of turbidites and debrites occurred in the E-W channel and trough axis channel. Cold seep communities,active fault and fluid flow were discovered along the lower slope of the Zenisu Ridge. Vertical sedimentary sequences in the Zenisu Trough consist of the four post-rift sequence units of the Shikoku Basin, among which Units A and B are two turbidite units. The development of Zenisu canyon is controlled by the N-S shear fault, the E-W fan channel is related to the E-W shear fault, and the trough-axis channel is related to the subsidence of central basin.
文摘Manganese nodules are deposit rich in manganese and other metals which "grow" on the ocean floor. The study of manganese nodule growth rate and deep sea sediment has both scientific and economic significance. Natural <sup>10</sup>Be （half-life= 1.5 Ma ）is produced from spallations of atmospheric nitrogen and oxygen induced by cosmic ray in the stratosphere. After several years of residence in the atmosphere, the <sup>10</sup>Be falls on the earth or deposits in the ocean, accumulating in marine
基金supported by the China Ocean Mineral Resources R&D Association(No.DYXM-115-02-2-01)the National Natural Science Foundation of China(No.40830213)the Hi-Tech Research and Development Program(863) of China(No.2007AA091904)
文摘The response of bacteria to various carbohydrates in the deep-sea sediments and the Antarctic soils was investigated using cellulose, chitin, and olive oil. It was found that the carbohydrates significantly increased the corresponding specific ectoenzyme activity （β- glucosidase, β-N-acetylglucosaminidase, lipase） in the samples from deep-sea sediments. In the case of Antarctic soil samples, the cellulose or olive oil amendments had minor or no effect on β-glucosidase or lipase activity, except the chitin which stimulated β- N-acetylglucosaminidase production. The responses of the bacteria in the deep-sea sediment sample WP02-3 and the Antarctic soil sample CC-TY2 towards the chitin amendment were further analyzed. Chitin amendments were shown to stimulate the ectoenzyme activity in all the tested sediments and the soils. The bacterial response before and after the carbohydrates amendments were compared by denaturing gradient gel electrophoresis and quantitative competitive polymerase chain reaction. Significant changes were found in the structure and density of the bacterial community in the deep sea sediments as compared to the Antarctic soil sample, where the effects were relatively lower. There was no change in the bacterial population in both studied samples in response to carbohydrates amendments. These data indicate that the bacterial communities in the oligotrophic deep-sea sediments are more dynamic than that in the Antarctic soils as they respond to the nutrient sources efficiently by regulation of ectoenzyme activity and/or changing community structure.
文摘以Web of Science引文数据库为检索对象,对1900-2012年间全球发表的深海资源相关论文进行文献计量学分析。从年度发文量与被引用量、主要论文著者、主要发文国家与机构等角度揭示深海资源研究发展轨迹。通过高频次关键词分析得出深海资源研究的5大问题是:生物物种、进化及多样性;沉积物;大洋环流与气候;有机物;生态环境、群落结构。从高被引与前沿热点分析得出深海沉积物与深海生物资源是其热点研究问题,且依然是未来深海资源研究的主要学科问题。
基金The research program was financially supported by the Joint Program of Chinese 863 Project (Grant No. 2001AA612020-2)
文摘A pressure tight sediment sampling technology, which can be introduced into the modification of the piston corer to accommodate the investigation of gas hydrate, is put forward. In this paper, the three basic rules of the technology are analyzed in detail: specimen transferring rule, seal self-tightening rule and semi-active pressure holding rule. Based on these, the structure of the pressure tight piston corer is put forward and its working principle is analyzed. Finally, a pressure tight sediment sampler, to which the same technology is applied, is researched through experiments. Results show that the sampler based on the above-mentioned theory has a good ability in sampling and in -situ pressure holding.
基金supported by the National Natural Science Foundation of China(40806021,40830107,and 91128208)the National Basic Research Program of China(2007CB815906)
文摘High resolution elemental records in core MD05-2903 from the northern South China Sea were obtained from XRF scanning and grain size and mineral component analyses.The K/Ti curve reveals climate changes since MIS 3 on a millennial scale,representing one of the best such records so far from deep-sea sediments of the South China Sea.When compared our K/Ti record with other climate records from high latitude ice cores and from East Asia stalagmites,variations in the K/Ti record show some typical saw-shaped features of rapid climate changes in the Northern Hemisphere.Grain size analysis reveals a close relationship between variations in K/Ti and in the fine end-member grain size.XRD analysis confirms that high K/Ti layers were dominated by K-rich weathering minerals,especially illite,while low K/Ti layers contain low illite but high abundance of Ti-rich heavy minerals.Therefore,the K/Ti record reflects changes in the input and deposition of fine weathering minerals and heavy minerals in the northern South China Sea region during stadials and interstadials.These elemental changes were largely controlled by variations in rainfall,erosion,and fluvial transportation induced by East Asia Monsoon.
文摘The South China Sea is the westernmost marginal sea in the west Pacific Ocean. On thebasis of the analyses of sedimentary components, biofossils, oxygen-carbon isotopes and theirdatings in four seafloor cores which were collected from the deep sea plain to continentalslope in the northern South China Sea, the environmental variations were basically similarto other tropical ocean areas, and their cycle changes in about the ages of 40 ka and 20 kawere more apparent since the late Pleistocene. During the warm climates, the depositionrate on the continental slope was about 2.6 times as rapid as on the deep sea plain margin.The seafloor in the deep sea plain was below the CaCO_3 compensation depth. During thecold periods, however, the deposition rate on the deep sea plain margin was slightly quickerthan on the continental slope due to the sea level reduction on a larger scale and moresupply of the terrigenous clastics.
