In this study,we examine the effects of various shapes of nanoparticles in a steady flow of hybrid nanofluids between two stretchable rotating disks.The steady flow of hybrid nanofluids with transformer oil as the bas...In this study,we examine the effects of various shapes of nanoparticles in a steady flow of hybrid nanofluids between two stretchable rotating disks.The steady flow of hybrid nanofluids with transformer oil as the base fluid and Fe_(3)O_(4)+TiO_(2)as the hybrid nanofluid is considered.Several shapes of Fe_(3)O_(4)+TiO_(2)hybrid nanofluids,including sphere,brick,blade,cylinder,and platelet,are studied.Every shape exists in the same volume of a nanoparticle.The leading equations(partial differential equations(PDEs))are transformed to the nonlinear ordinary differential equations(ODEs)with the help of similarity transformations.The system of equations takes the form of ODEs depending on the boundary conditions,whose solutions are computed numerically by the bvp4c MATLAB solver.The outputs are compared with the previous findings,and an intriguing pattern is discovered,such that the tangential velocity is increased for the rotation parameter,while it is decreased by the stretching values because of the lower disk.For the reaction rate parameter,the concentration boundary layer becomes shorter,and the activation energy component increases the rate at which mass transfers come to the higher disk but have the opposite effect on the bottom disk.The ranges of various parameters taken into account are Pr=6.2,Re=2,M=1.0,φ_(1)=φ_(2)=0.03,K=0.5,S=-0.1,Br=0.3,Sc=2.0,α_(1)=0.2,γ=0.1,E_(n)=2.0,and q=1.0,and the rotation factor K is within the range of 0 to 1.展开更多
Objective: Increasing the emergence of Metallo-β-lactamase (MBL) producing gram-negative bacteria and their dexterous horizontal transmission demands rapid and accurate detection. This study was conducted to determin...Objective: Increasing the emergence of Metallo-β-lactamase (MBL) producing gram-negative bacteria and their dexterous horizontal transmission demands rapid and accurate detection. This study was conducted to determine a suitable method to promptly detect MBL-producing gram-negative bacteria. Methods: A total of 103 gram-negative bacteria were identified from various clinical samples at a tertiary care hospital in Dhaka city. MBL producers were detected by two phenotypic methods, the Disk Potentiation Test (DPT) and the Double Disk Synergy Test (DDST) based on β-lactam chelator combinations where EDTA/SMA has been used as an inhibitor and Imipenem, Ceftazidime as substrates. Results: 103 isolates which were identified as Escherichia coli spp, Klebsiella spp, Pseudomonas spp, Acinetobacter spp, Proteus spp, Providencia spp were found to be multidrug-resistant in antibiogram test. Isolates showed complete resistance (100%) to Imipenem, Meropenem, and Amoxiclav. The highest carbapenem-resistant etiological agents were Acinetobacter spp 40 (38.8%) followed by Pseudomonas spp 27 (26.2%), Klebsiella spp 26 (25.2%), Escherichia coli 8 (7.8%), Proteus spp 1 (1%) and Providencia spp 1 (1%). DPT method detected significantly (p = 0.000009) a higher number of MBL-producers (Imipenem with 0.5 M EDTA n = 61, 59.2% & Ceftazidime with 0.5 M EDTA n = 56, 54.4%) compared to the DDST method (Imipenem -0.5 M EDTA n = 43, 41.7%, Imipenem – SMA n = 38, 36.9% & Ceftazidime -0.5 M EDTA n = 15, 14.6%). Conclusion: Pieces of evidence suggest that DPT is a more sensitive method than DDST and could be recommended for identifying MBL-producing bacteria in Bangladeshi hospitals for the proper management of patients, to reduce time constraints and treatment costs.展开更多
An analytical solution is obtained for the flow due to solid-body rotations an oscillating porous disk and of a fluid at infinity. Neglecting the induced magnetic field, the effects of the transversely applied magneti...An analytical solution is obtained for the flow due to solid-body rotations an oscillating porous disk and of a fluid at infinity. Neglecting the induced magnetic field, the effects of the transversely applied magnetic field on the flow are studied. Further, the flow confined between two disks is also discussed. It is found that an infinite number of solutions exist for the flow confined between two disks.展开更多
This study was conducted to assess the occurrence and nature of extended-spectrum beta lactamase (ESBL) producing <em>Escherichia coli</em> and <em>Klebsiella pneumoniae</em> isolates from pati...This study was conducted to assess the occurrence and nature of extended-spectrum beta lactamase (ESBL) producing <em>Escherichia coli</em> and <em>Klebsiella pneumoniae</em> isolates from patients who presented with urinary tract infection at Federal Teaching Hospital Gombe. Isolates collected were recovered on MacConkey agar at 35<span style="white-space:nowrap;">°</span>C and were identified as members of Enterobacteriaceae, and further screened for antimicrobial susceptibility and resistance by disc diffusion method. Isolates resistant to oxyimino-cephalosporins were confirmed as ESBL producers using Double Disks Synergy Test (DDST). The study shows 66% resistance to ceftriaxone (30 μg) in <em>K. pneumoniae</em>, which was the highest value recorded and a 51% resistance to cefpodoxime (10 <em>μ</em>g) in <em>E. coli</em>. The sensitivity of <em>E. coli </em>and <em>K. pneumoniae</em> isolates to cefpodoxime (10 <em>μ</em>g) were 49% and 33.9% respectively. ESBLs were detected among 40% (40/100) of <em>E. coli</em> and 54.13% (59/109) of <em>K. pneumoniae</em> isolates. Molecular characterization of ESBL encoding genes among <em>E. coli</em> isolates using multiplex-PCR showed 10% prevalence of SHV gene and 5% prevalence for CTX-M gene while TEM gene was not detected. In <em>K. pneumoniae</em> isolates, 5% prevalence was recorded for each of the three genes screened. The study revealed a co-occurrence of SHV and CTX-M in 75% of the <em>E. coli</em> and 70% of the <em>K. pneumoniae</em> isolates;the occurrence of all the three genes was seen in 10% and 5% of <em>K. pneumoniae</em> and <em>E. coli</em> respectively. Multiplex-PCR method provided an efficient and rapid detection of ESBL related genes, hence could be used in epidemiological studies among ESBL isolates. Monitoring dissemination and transmissions of ESBL producers are highly recommended for optimum patient care and preventing the spread of multidrug resistant (MDR) pathogens.展开更多
文摘In this study,we examine the effects of various shapes of nanoparticles in a steady flow of hybrid nanofluids between two stretchable rotating disks.The steady flow of hybrid nanofluids with transformer oil as the base fluid and Fe_(3)O_(4)+TiO_(2)as the hybrid nanofluid is considered.Several shapes of Fe_(3)O_(4)+TiO_(2)hybrid nanofluids,including sphere,brick,blade,cylinder,and platelet,are studied.Every shape exists in the same volume of a nanoparticle.The leading equations(partial differential equations(PDEs))are transformed to the nonlinear ordinary differential equations(ODEs)with the help of similarity transformations.The system of equations takes the form of ODEs depending on the boundary conditions,whose solutions are computed numerically by the bvp4c MATLAB solver.The outputs are compared with the previous findings,and an intriguing pattern is discovered,such that the tangential velocity is increased for the rotation parameter,while it is decreased by the stretching values because of the lower disk.For the reaction rate parameter,the concentration boundary layer becomes shorter,and the activation energy component increases the rate at which mass transfers come to the higher disk but have the opposite effect on the bottom disk.The ranges of various parameters taken into account are Pr=6.2,Re=2,M=1.0,φ_(1)=φ_(2)=0.03,K=0.5,S=-0.1,Br=0.3,Sc=2.0,α_(1)=0.2,γ=0.1,E_(n)=2.0,and q=1.0,and the rotation factor K is within the range of 0 to 1.
文摘Objective: Increasing the emergence of Metallo-β-lactamase (MBL) producing gram-negative bacteria and their dexterous horizontal transmission demands rapid and accurate detection. This study was conducted to determine a suitable method to promptly detect MBL-producing gram-negative bacteria. Methods: A total of 103 gram-negative bacteria were identified from various clinical samples at a tertiary care hospital in Dhaka city. MBL producers were detected by two phenotypic methods, the Disk Potentiation Test (DPT) and the Double Disk Synergy Test (DDST) based on β-lactam chelator combinations where EDTA/SMA has been used as an inhibitor and Imipenem, Ceftazidime as substrates. Results: 103 isolates which were identified as Escherichia coli spp, Klebsiella spp, Pseudomonas spp, Acinetobacter spp, Proteus spp, Providencia spp were found to be multidrug-resistant in antibiogram test. Isolates showed complete resistance (100%) to Imipenem, Meropenem, and Amoxiclav. The highest carbapenem-resistant etiological agents were Acinetobacter spp 40 (38.8%) followed by Pseudomonas spp 27 (26.2%), Klebsiella spp 26 (25.2%), Escherichia coli 8 (7.8%), Proteus spp 1 (1%) and Providencia spp 1 (1%). DPT method detected significantly (p = 0.000009) a higher number of MBL-producers (Imipenem with 0.5 M EDTA n = 61, 59.2% & Ceftazidime with 0.5 M EDTA n = 56, 54.4%) compared to the DDST method (Imipenem -0.5 M EDTA n = 43, 41.7%, Imipenem – SMA n = 38, 36.9% & Ceftazidime -0.5 M EDTA n = 15, 14.6%). Conclusion: Pieces of evidence suggest that DPT is a more sensitive method than DDST and could be recommended for identifying MBL-producing bacteria in Bangladeshi hospitals for the proper management of patients, to reduce time constraints and treatment costs.
文摘An analytical solution is obtained for the flow due to solid-body rotations an oscillating porous disk and of a fluid at infinity. Neglecting the induced magnetic field, the effects of the transversely applied magnetic field on the flow are studied. Further, the flow confined between two disks is also discussed. It is found that an infinite number of solutions exist for the flow confined between two disks.
文摘This study was conducted to assess the occurrence and nature of extended-spectrum beta lactamase (ESBL) producing <em>Escherichia coli</em> and <em>Klebsiella pneumoniae</em> isolates from patients who presented with urinary tract infection at Federal Teaching Hospital Gombe. Isolates collected were recovered on MacConkey agar at 35<span style="white-space:nowrap;">°</span>C and were identified as members of Enterobacteriaceae, and further screened for antimicrobial susceptibility and resistance by disc diffusion method. Isolates resistant to oxyimino-cephalosporins were confirmed as ESBL producers using Double Disks Synergy Test (DDST). The study shows 66% resistance to ceftriaxone (30 μg) in <em>K. pneumoniae</em>, which was the highest value recorded and a 51% resistance to cefpodoxime (10 <em>μ</em>g) in <em>E. coli</em>. The sensitivity of <em>E. coli </em>and <em>K. pneumoniae</em> isolates to cefpodoxime (10 <em>μ</em>g) were 49% and 33.9% respectively. ESBLs were detected among 40% (40/100) of <em>E. coli</em> and 54.13% (59/109) of <em>K. pneumoniae</em> isolates. Molecular characterization of ESBL encoding genes among <em>E. coli</em> isolates using multiplex-PCR showed 10% prevalence of SHV gene and 5% prevalence for CTX-M gene while TEM gene was not detected. In <em>K. pneumoniae</em> isolates, 5% prevalence was recorded for each of the three genes screened. The study revealed a co-occurrence of SHV and CTX-M in 75% of the <em>E. coli</em> and 70% of the <em>K. pneumoniae</em> isolates;the occurrence of all the three genes was seen in 10% and 5% of <em>K. pneumoniae</em> and <em>E. coli</em> respectively. Multiplex-PCR method provided an efficient and rapid detection of ESBL related genes, hence could be used in epidemiological studies among ESBL isolates. Monitoring dissemination and transmissions of ESBL producers are highly recommended for optimum patient care and preventing the spread of multidrug resistant (MDR) pathogens.
