In order to contribute to a better understanding of the biodiversity of local chicken populations, this study focused on the description of the essential qualitative parameters in the phenotypic characterization of lo...In order to contribute to a better understanding of the biodiversity of local chicken populations, this study focused on the description of the essential qualitative parameters in the phenotypic characterization of local species. Conducted in 6 localities in the Far North Region of Cameroon (Doukoula, Yagoua, Guidiguis, Dziguilao, Maroua 3rd and Méri), a population of 240 local chickens, including 172 hens and 68 cocks were characterized in family farms. The choice of localities was made on the basis of their strong potential local chicken flocks in the region. To this end, each animal was the subject of a qualitative description based on the identification of the sex of the animal, the description of the colorations of the plumage and extremities, and the description of the types of format, plumage and crests. All observations were made with the naked eye and in daylight and then photographed. The main results show that the plumage colors are multiple and dominated by the White-Pied-Black (21.7%) and the Mille-fleur (20.8%);the wattles are dominated by the red (60%) and the pink (35.4%);the tarsi are dominated by the white (43.8%) and the black (32.08%);the white skin (92.5%) dominates over the pink skin (7.5%);the “Smooth-uniform” feather is dominant (97.08%), followed by the smooth-crested type (2.08%) and the fries type (0.82%);the medium size of the chickens is dominant (86.66%), followed by the dwarf size (9.58%) and the large size (3.75%). The results of this study demonstrate that there is a strong phenotypic diversity within the local chicken population. This diversity can serve as a basis for the development of selection, conservation and genetic potential improvement programs based on rational exploitation of the local chicken.展开更多
Weather forecasts from numerical weather prediction models play a central role in solar energy forecasting,where a cascade of physics-based models is used in a model chain approach to convert forecasts of solar irradi...Weather forecasts from numerical weather prediction models play a central role in solar energy forecasting,where a cascade of physics-based models is used in a model chain approach to convert forecasts of solar irradiance to solar power production.Ensemble simulations from such weather models aim to quantify uncertainty in the future development of the weather,and can be used to propagate this uncertainty through the model chain to generate probabilistic solar energy predictions.However,ensemble prediction systems are known to exhibit systematic errors,and thus require post-processing to obtain accurate and reliable probabilistic forecasts.The overarching aim of our study is to systematically evaluate different strategies to apply post-processing in model chain approaches with a specific focus on solar energy:not applying any post-processing at all;post-processing only the irradiance predictions before the conversion;post-processing only the solar power predictions obtained from the model chain;or applying post-processing in both steps.In a case study based on a benchmark dataset for the Jacumba solar plant in the U.S.,we develop statistical and machine learning methods for postprocessing ensemble predictions of global horizontal irradiance(GHI)and solar power generation.Further,we propose a neural-network-based model for direct solar power forecasting that bypasses the model chain.Our results indicate that postprocessing substantially improves the solar power generation forecasts,in particular when post-processing is applied to the power predictions.The machine learning methods for post-processing slightly outperform the statistical methods,and the direct forecasting approach performs comparably to the post-processing strategies.展开更多
Dryopteridaceae are the largest fern family and include nearly 20%of extant fern diversity,with 24 currently recognized genera.Recognition and delineation of genera within this family have varied greatly.The three-sub...Dryopteridaceae are the largest fern family and include nearly 20%of extant fern diversity,with 24 currently recognized genera.Recognition and delineation of genera within this family have varied greatly.The three-subfamily classification of Dryopteridaceae was based primarily on molecular phylogenetic relationships but lacked morphological evidence,and the phylogenetic relationships of the subfamilies and genera of Dryopteridaceae are only partially resolved.A comprehensive and robust phylogeny is urgently needed.The heterogeneous morphology of the current members of Dryopteridaceae makes the family and its subfamilies difficult to define by single morphological characteristics or even character combinations.We carried out phylogenetic analyses to reconstruct a highly supported phylogeny of Dryopteridaceae.Our analyses recovered 24 strongly supported clades grouped into seven major clades of Dryopteridaceae.Seven morphological characters including habit,rhizome shape,frond morphology,rachis-costae architecture,appendages on stipe base and lamina,and soral arrangement were found to be informative for identifying different major clades and clades in Dryopteridaceae.Based on phylogenetic reconstruction and morphological analysis,we presented an updated infra-familial classification of Dryopteridaceae with seven subfamilies and 24 genera including four newly proposed subfamilies(Ctenitidoideae,Lastreopsidoideae,Pleocnemioideae,and Polystichopsidoideae).Morphological character combinations of each subfamily are summarized,and a key is provided.Most genera sensu PPG I are recognized,with Stigmatopetris reclassified into Dryopteridoideae and Arthrobotrya considered a synonym of Teratophyllum.A new genus Pseudarachniodes is introduced.This revised classification will serve as a foundational framework for future investigations on taxonomy,biogeography,and diversification of the most species-rich Dryopteridaceae in ferns.展开更多
A morphology-based growth stage system should describe the growth and development of a crop and thereby help farmers and agronomists in formulating reasonable managementmeasures conducive to the development of marketa...A morphology-based growth stage system should describe the growth and development of a crop and thereby help farmers and agronomists in formulating reasonable managementmeasures conducive to the development of marketable products.However,existing growth stage systems for soybean are either based on plant growth or covered particular phases of flower or pod development,making it difficult to use for tracking the entire growth period of individual flowers and pods.Therefore,the first flower and pod,located at the base of the primary raceme in the eighth trifoliate node of the main stem,were chosen to illustrate growth dynamics during the full reproductive period.The size and fresh weight of the primary raceme in the eighth trifoliate leaf axil,the first flower and pod,the pistils in the first flower,and seeds in the first pod were examined,and the growth of these organs was depicted.Integrating the morphological characteristics and growth features of flowers and pods,as well as existing growth stage systems,the growth and development were delineated in 13 stages.In detail,we classified the flower phase based on the relative positions of floral components,inspired by the ratio of bract to flower used for staging,refined the lag phase proposed previously,retained the use of pod length to define the early pod phase,and innovatively described the late pod phase by the seed appearance.The developmental events in each stage of flower and pod were distinctive and closely connected to the corresponding morphology.Taken together,a more detailed growth stage system for describing individual flowers and pods in soybean was established.This system will serve as a valuable research tool for describing the development,gene expression,and cellular metabolism associated with the formation of flowers,pods,and seeds.展开更多
Understanding the ecogeographic mechanisms driving morphological variation is pivotal in biogeographic studies. However, patterns and determinants of such variation remain contentious, particularly in amphibians. Alth...Understanding the ecogeographic mechanisms driving morphological variation is pivotal in biogeographic studies. However, patterns and determinants of such variation remain contentious, particularly in amphibians. Although several hypotheses have been formulated and investigated in amphibians, their validity remains disputed with mixed support. Using the Sichuan spiny frog(Nanorana sichuanensis) as an indicator, we investigated the morphological variations across geographic and environmental gradients to explore the underlying ecogeographic mechanisms. We found that both the body size and limb characteristics of N.sichuanensis were not significantly related to latitude or elevation, suggesting that it did not follow Bergmann's or Allen's rules. Eye diameter decreased linearly with increasing elevation and latitude, whereas snout length increased with increasing elevation and latitude. Heat balance, endurance, seasonality, water availability, and primary productivity collectively explain body size variation. Hierarchical partitioning identified primary productivity and thermal excursion as the most influential factors, explaining significant variability in body size and other morphological features. Specifically, primary productivity accounted for 52.40% of the variation in body size, whereas thermal excursion had the greatest impact on eye diameter(36.23%) and snout length(72.17%). Based on body size and dimensionally reduced morphological features, our results identified ecogeographic patterns, assessed the validity of different hypotheses, and examined how environmental factors influence these morphological variations. More generally, our study offers comprehensive insights into the ecogeographic variation observed in mountain amphibians,provides a critical evaluation of existing ecogeographic hypotheses, and infers possible morphological adaptations in response to environmental change.展开更多
The Salmonella pathogenicity islands(SPIs) play crucial roles in the progression of Salmonella infection. In this study, we constructed an improved λ Red homologous recombination system to prepare single and triple d...The Salmonella pathogenicity islands(SPIs) play crucial roles in the progression of Salmonella infection. In this study, we constructed an improved λ Red homologous recombination system to prepare single and triple deletion mutants of 3 prominent SPIs(SPI-1, 2, and 3), aiming at the impact of deletion on morphology, carbon source metabolism, adhesion and invasion capacity, in vivo colonization, and immune efficacy in chicks. Our examination revealed that the surface of the single deletion mutants(SM6ΔSPI1, ΔSPI2, and ΔSPI3) exhibited a more rugged texture and appeared to be enveloped in a layer of transparent colloid, whereas the morphology of the triple deletion mutant(SM6ΔSPI1&2&3) remained unaltered when compared to the parent strain. The carbon metabolic spectrum of the SPI mutants underwent profound alterations, with a notable and statistically significant modification observed in 30 out of 95 carbon sources, primarily carbohydrates(17 out of 30). Furthermore, the adhesion capacity of the 4 mutants to Caco-2 cells was significantly reduced when compared to that of the parent strain. Moreover,the invasion capacity of mutants SM6ΔSPI1 and SM6ΔSPI1&2&3 exhibited a substantial decrease, while it was enhanced to varying degrees for SM6ΔSPI3 and SM6ΔSPI2. Importantly, none of the 4 mutants induced any clinical symptoms in the chicks. However, they did transiently colonize the spleen and liver. Notably, the SM6ΔSPI1&2&3mutant was rapidly cleared from both the spleen and liver within 8 days post-infection and no notable pathological changes were observed in the organs. Additionally, when challenged, the mutants immunized groups displayed a significant increase in antibody levels and alterations in the CD3+CD4+ and CD3+CD8+ subpopulations, and the levels of IL-4 and IFN-γ cytokines in the SM6ΔSPI1&2&3 immunized chicken serum surpassed those of other groups.In summary, the successful construction of the 4 SPI mutants lays the groundwork for further exploration into the pathogenic(including metabolic) mechanisms of SPIs and the development of safe and effective live attenuated Salmonella vaccines or carriers.展开更多
Assessing the threat status of species in response to global change is critical for biodiversity monitoring and conservation efforts.However,current frameworks,even the IUCN Red List,often neglect critical factors suc...Assessing the threat status of species in response to global change is critical for biodiversity monitoring and conservation efforts.However,current frameworks,even the IUCN Red List,often neglect critical factors such as genetic diversity and the impacts of climate and land-use changes,hindering effective conservation planning.To address these limitations,we developed an enhanced extinction risk assessment framework using Diploderma lizards as a model.This framework incorporates long-term field surveys,environmental data,and land-use information to predict distributional changes for 10 recently described Diploderma species on the Qinghai-Xizang Plateau,which hold ecological significance but remain underassessed in conservation assessment.By integrating the distribution data and genetically inferred effective population sizes(Ne),we conducted scenario analyses and used a rank-sum approach to calculate Risk ranking scores(RRS)for each species.This approach revealed significant discrepancies with the IUCN Red List assessments.Notably,D.yangi and D.qilin were identified as facing the highest extinction risk.Furthermore,D.vela,D.batangense,D.flaviceps,D.dymondi,D.yulongense,and D.laeviventre,currently classified as“Least Concern”,were found to warrant reclassification as“Vulnerable”due to considerable threat from projected range contractions.Exploring the relationship between morphology and RRS revealed that traits such as snout-vent length and relative tail length could serve as potential predictors of extinction risk,offering preliminary metrics for assessing species vulnerability when comprehensive data are unavailable.This study enhances the precision of extinction risk assessment frameworks and demonstrates their capacity to refine and update risk assessments,especially for lesser-known taxa.展开更多
WITHDRAWAL:Zhang,J.J.,Guo,Y.Q.,Qin,Z.Y.,Wei,C.T.,Hu,Q.H.,Vandeginste,V.,Miao,H.Y.,Yao,P.,and Zhang,P.F.,“Predicting Irreducible Water Saturation of Unconventional Reservoirs by Using NMR T2 Spectra:Methods of Morphol...WITHDRAWAL:Zhang,J.J.,Guo,Y.Q.,Qin,Z.Y.,Wei,C.T.,Hu,Q.H.,Vandeginste,V.,Miao,H.Y.,Yao,P.,and Zhang,P.F.,“Predicting Irreducible Water Saturation of Unconventional Reservoirs by Using NMR T2 Spectra:Methods of Morphological Division and Fractal Models”,Acta Geologica Sinica-English Edition(Accepted Article):https://doi.org/10.1111/1755-6724.15094.展开更多
Autophagy is a cellular catabolic process characterized by the formation of double-membrane autophagosomes.Transmission electron microscopy is the most rigorous method to clearly visualize autophagic engulfment and de...Autophagy is a cellular catabolic process characterized by the formation of double-membrane autophagosomes.Transmission electron microscopy is the most rigorous method to clearly visualize autophagic engulfment and degradation.A large number of studies have shown that autophagy is closely related to the digestion,secretion,and regeneration of gastrointestinal(GI)cells.However,the role of autophagy in GI diseases remains controversial.This article focuses on the morphological and biochemical characteristics of autophagy in GI diseases,in order to provide new ideas for their diagnosis and treatment.展开更多
In our recent investigations of diatom diversity,we studied three species,namely,Skeletonema costatum,Skeletonema subsalsum,and Skeletonema potamos.Although they have been found frequently in Changjiang(Yangtze)River ...In our recent investigations of diatom diversity,we studied three species,namely,Skeletonema costatum,Skeletonema subsalsum,and Skeletonema potamos.Although they have been found frequently in Changjiang(Yangtze)River Basin,their morphological and molecular identification is difficult in taxonomy.Therefore,to integrate morphological and molecular biological approaches,we compared systematically their morphological characters and performed phylogenetic analysis.Twelve strains of Skeletonema were collected and isolated from Shanghai and Jiangsu,China,and their morphological characteristics were examined by light microscopy(LM)and the scanning electron microscopy(SEM).Based on morphological comparison,we determined that S.potamos is easy to distinguish from the other two species.The heavily silicified areolae,undulated or cleft distal ends of terminal fultoportula processes(TFPPs),absence of basal pores of fultoportula processes(FPPs),the rootlike protrusions of FPPs,and no interlocking connection are the stable characteristics that can be used to identify S.potamos.However,there are only two features that can distinguish S.costatum from S.subsalsum,namely the location of terminal rimoportulae(TRPs)and the distal shape of TFPPs.In addition,we amplified and sequenced nine common genetic markers from the strains,from which 101 sequences were obtained,constructed phylogenetic trees based on the nine genes and evaluated that seven genes can be used to identify S.potamos,and revealed that S.subsalsum is the closest known relative of S.costatum,and only ATP synthetase beta-subunit gene(atp B)is able to distinguish them from each other,which strongly support that it is an effective molecular marker for Skeletonema.This work provided a theoretical basis for the taxonomic study of Skeletonema.展开更多
Country bean, Lablab purpureus (L.) is considered one of the most important leguminous crops, but their cultivation under drought stress condition encounters challenges. In this study, an experiment has been conducted...Country bean, Lablab purpureus (L.) is considered one of the most important leguminous crops, but their cultivation under drought stress condition encounters challenges. In this study, an experiment has been conducted among 30 genotypes under drought condition to explore morphological diversity of qualitative and quantitative, biochemical, molecular analysis. The study identified significant variations in eight traits among the genotypes examined, with phenotypic variance exceeding genotypic variance, indicating both genetic and environmental influences. High heritability and genetic advance were observed in primary, secondary, and tertiary branch lengths, suggesting these traits are likely controlled by additive gene effects, making them effective targets for selection. Principal component analysis identified three components that made a substantial contribution, accounting for approximately 73.06% of the overall quantitative variations. Among the quantitative traits, the highest coefficient of variation (CV%) has been found in number of flowers (55.05%). While number of primary branches, primary branch length, number of secondary branches, secondary branch length, number of tertiary branches, tertiary branch length has individually more than 20% of CV%. The genotypes have been grouped into three clusters based on quantitative traits. Analysis of protein reveals that the genotypes of DS28 and DS29 have higher protein content than other genotypes. Dehydrogenase responsive genotypes have been found on DS28 and DS29 from the molecular analysis. The results suggest that the genotypes DS28 and DS29 could contribute as genetic resource of high protein content and DREB responsive, and the eight quantitative traits of 30 genotypes could be used for further breeding programme.展开更多
In this study, 32 black rice genotypes spanning diverse geographies in India were evaluated at both phenotypic and DNA sequence levels to gain insights into their genetic makeup. Significant variations were observed f...In this study, 32 black rice genotypes spanning diverse geographies in India were evaluated at both phenotypic and DNA sequence levels to gain insights into their genetic makeup. Significant variations were observed for 14 agro-morphological traits, showing correlations among several yield-related traits.展开更多
In overlapping distribution areas of Sorbus pohuashanensis and S.discolor in North China(Mount Tuoliang,Mount Xiling and Mount Baihua),Sorbus indi-viduals were found with pink fruit,which have never been recorded for ...In overlapping distribution areas of Sorbus pohuashanensis and S.discolor in North China(Mount Tuoliang,Mount Xiling and Mount Baihua),Sorbus indi-viduals were found with pink fruit,which have never been recorded for the flora of China.Fourteen morphological characters combined with four chloroplast DNA markers and internal transcribed spacer(ITS)were used to analyze the origin of the Sorbus individuals with pink fruits and their relationship to S.pohuashanensis and S.discolor.PCA,SDA and one-way(taxon)ANOVA of morphological characters provided convincing evidence of the hybrid ori-gin of Sorbus individuals with pink fruits based on a novel morphological character and many intermediate characters.Haplotype analysis based on four cpDNA markers showed that either S.pohuashanensis or S.discolor were maternal parents of Sorbus individuals with pink fruits.Incongru-ence of the position of Sorbus individuals with pink fruits between cpDNA and ITS in cluster trees supported by DNA sequence comparative analysis,implying former hybridiza-tion events between S.pohuashanensis and S.discolor.Mul-tiple hybridization events between S.pohuashanensis and S.discolor might have contributed to the generation of Sorbus individuals with pink fruits.This study has provided insights into hybridization between species of the same genus in sympatric areas,which is of great significance for the study of interspecific hybridization.展开更多
Phlomoides,with 150-170 species,is the second largest and perhaps most taxonomically challenging genus within the subfamily Lamioideae(Lamiaceae).With about 60 species,China is one of three major biodiversity centers ...Phlomoides,with 150-170 species,is the second largest and perhaps most taxonomically challenging genus within the subfamily Lamioideae(Lamiaceae).With about 60 species,China is one of three major biodiversity centers of Phlomoides.Although some Phlomoides species from China have been included in previous molecular phylogenetic studies,a robust and broad phylogeny of this lineage has yet to be completed.Moreover,given the myriad new additions to the genus,the existing infrageneric classification needs to be evaluated and revised.Here,we combine molecular and morphological data to investigate relationships within Phlomoides,with a focus on Chinese species.We observed that plastid DNA sequences can resolve relationships within Phlomoides better than nuclear ribosomal internal and external transcribed spacer regions(nrITS and nrETS).Molecular phylogenetic analyses confirm the monophyly of Phlomoides,but most previously defined infrageneric groups are not monophyletic.In addition,morphological analysis demonstrates the significant taxonomic value of eight characters to the genus.Based on our molecular phylogenetic analyses and morphological data,we establish a novel section Notochaete within Phlomoides,and propose three new combinations as well as three new synonyms.This study presents the first molecular phylogenetic analyses of Phlomoides in which taxa representative of the entire genus are included,and highlights the phylogenetic and taxonomic value of several morphological characters from species of Phlomoides from China.Our study suggests that a taxonomic revision and reclassification for the entire genus is necessary in the future.展开更多
Glacier inventories serve as critical baseline data for understanding the impacts of climate change on glaciers.The present study maps the outlines of glaciers in the Chandra-Bhaga Basin(western Himalaya)for the years...Glacier inventories serve as critical baseline data for understanding the impacts of climate change on glaciers.The present study maps the outlines of glaciers in the Chandra-Bhaga Basin(western Himalaya)for the years 1993,2000,2010,and 2019 using Landsat Thematic Mapper(TM),Enhanced Thematic Mapper(ETM),and Operational Land Imager(OLI)datasets.A total of 251 glaciers,each having an area above 0.5 km^(2),were identified,which include 216 clean-ice and 35 debris-covered glaciers.Area changes are estimated for three periods:1993-2000,2000-2010,and 2010-2019.The total glacierized area was 996±62 km^(2) in 1993,which decreased to 973±70 km^(2) in 2019.The mean rate of glacier area loss was higher in the recent decade(2010-2019),at 0.036 km^(2),compared to previous decades(0.029 km^(2) in 2000-2010 and 0.025 km^(2) in 1993-2000).Supraglacial debris cover changes are also mapped over the period of 1993 and 2019.It is found that the supraglacial debris cover increased by 14.12±2.54 km^(2)(15.2%)during 1993-2019.Extensive field surveys on Chhota Shigri,Panchi II,Patsio,Hamtah,Mulkila,and Yoche Lungpa glaciers were carried out to validate the glacier outlines and supraglacial debris cover estimated using satellite datasets.Controls of various morphological parameters on retreat were also analyzed.It is observed that small,clean ice,south oriented glaciers,and glaciers with proglacial lakes are losing area at faster rates than other glaciers in the basin.展开更多
Light-sheet fluorescence microscopy(LSFM)has been widely used to image the three-dimensional(3D)structures and functions of various millimeter-size bio-specimen such as zebrafish.However,the sample adsorption and scat...Light-sheet fluorescence microscopy(LSFM)has been widely used to image the three-dimensional(3D)structures and functions of various millimeter-size bio-specimen such as zebrafish.However,the sample adsorption and scattering cause shading of the light-sheet illumination,preventing the even 3D image of thick samples.Herein,we report a continuous-rotational light-sheet microscope(CR-LSM)that enables simultaneous 3D bright-field and fluorescence imaging.With a high-accuracy rotational stage,CR-LSM records the outline projections and the fluorescent images of the sample at multiple rotation angles.Then,3D morphology and fluorescent structure were reconstructed with a developed algorithm.Using CR-LSM,zebrafish’s whole-fish contour and blood vessel structures were obtained simultaneously.展开更多
A fractal geometric boundary with natural wall features is introduced into a hybrid lattice-Boltzmann-method(LBM)multiphase model. The physical model of cavitation bubble collapse near the irregular geometric wall is ...A fractal geometric boundary with natural wall features is introduced into a hybrid lattice-Boltzmann-method(LBM)multiphase model. The physical model of cavitation bubble collapse near the irregular geometric wall is established to study the thermodynamic characteristics of the bubble collapse. Due to the lack of periodicity, symmetry, spatial uniformity and obvious correlation in the LBM simulation of the bubble collapse near the fractal wall, the morphological analysis based on Minkowski functional is introduced into the thermodynamic investigation of cavitation bubble so as to analyze and obtain the effective information. The results show that the Minkowski functional method can employed to study the temperature information in complex physical fields hierarchically and quantitatively. The high/low temperature region of the cavitation flow is explored, and thermal effect between irregular and fractal geometric wall and cavitation bubble can be revealed. It illustrates that LBM and morphological analysis complement each other, and morphological analysis can also be used as an optional and potential tool in research field of complex multiphase flows.展开更多
This study evaluated the variation in yellow root cassava (Manihot esculentus Crantz) genotypes and phenotypic relationship for selected postharvest and morphological traits. The trial was established at the Njala Agr...This study evaluated the variation in yellow root cassava (Manihot esculentus Crantz) genotypes and phenotypic relationship for selected postharvest and morphological traits. The trial was established at the Njala Agricultural Research Centre experimental site, Njala, during 2017/2018 cropping season in a randomized complete block design with three replications. Findings showed that the higher the total carotene content (TCC) in yellow flesh cassava genotypes, the longer the rate of postharvest physiological deterioration (PPD). Genotypes TR-0051-TCC/17 and TR-0012-TCC/17 recorded higher TCC (18.9 µg/g and 13.6 µg/g) and longer rate of PPD (4.29 and 3.14), respectively. Genotypes TR-0051-TCC/17, TR-0016-TCC/17, TR-0028-TCC/17, TR-0012-TCC/17 and TR-0020-TCC/17 had the highest TCC values of 18.9 µg/g, 16.09 µg/g, 14.72 µg/g, 13.6 µg/g and 11.23 µg/g with corresponding higher color chart values of 6, 6, 6, 5, and 6, respectively. This suggests the direct dependence of TCC on the root parenchyma color intensity in yellow flesh cassava genotypes. Findings also show a direct relationship between morphological and postharvest traits in yellow flesh cassava genotypes that could be exploited for the genetic improvement of cassava for increased shelf life, nutrition and related quality traits, as well as conservation and utilization of the crop.展开更多
AIM:To quantitatively measure ocular morphological parameters of guinea pig with Python technology.METHODS:Thirty-six eyeballs of eighteen 3-weekold guinea pigs were measured with keratometer and photographed to obtai...AIM:To quantitatively measure ocular morphological parameters of guinea pig with Python technology.METHODS:Thirty-six eyeballs of eighteen 3-weekold guinea pigs were measured with keratometer and photographed to obtain the horizontal,coronal,and sagittal planes respectively.The corresponding photo pixels-actual length ratio was acquired by a proportional scale.The edge coordinates were identified artificially by ginput function.Circle and conic curve fitting were applied to fit the contour of the eyeball in the sagittal,coronal and horizontal view.The curvature,curvature radius,eccentricity,tilt angle,corneal diameter,and binocular separation angle were calculated according to the geometric principles.Next,the eyeballs were removed,canny edge detection was applied to identify the contour of eyeball in vitro.The results were compared between in vivo and in vitro.RESULTS:Regarding the corneal curvature and curvature radius on the horizontal and sagittal planes,no significant differences were observed among results in vivo,in vitro,and the keratometer.The horizontal and vertical binocular separation angles were 130.6°±6.39°and 129.8°±9.58°respectively.For the corneal curvature radius and eccentricity in vivo,significant differences were observed between horizontal and vertical planes.CONCLUSION:The Graphical interface window of Python makes up the deficiency of edge detection,which requires too much definition in Matlab.There are significant differences between guinea pig and human beings,such as exotropic eye position,oblique oval eyeball,and obvious discrepancy of binoculus.This study helps evaluate objectively the ocular morphological parameters of small experimental animals in emmetropization research.展开更多
Lake is an important part of the natural ecosystem, and its morphological characteristics reflect the capacity of lake regulation and storage, the strength of material migration, and the characteristics of shoreline d...Lake is an important part of the natural ecosystem, and its morphological characteristics reflect the capacity of lake regulation and storage, the strength of material migration, and the characteristics of shoreline development. In most existing studies, remote sensing images are used to quantify the morphological characteristics of lakes. However, the extraction accuracy of lake water is greatly affected by cloud cover and vegetation cover, and the inversion accuracy of lake elevation data is poor, which cannot accurately describe the response relationship of lake landscape morphology with water level change. Therefore, this paper takes Tonle Sap Lake as the research object, which is the largest natural freshwater lake in Southeast Asia. DEM is constructed based on high-resolution measured topographic data, and morphological indicators such as lake area, lake shoreline length, perimeter area ratio, longest axis length, maximum width, shoreline development index, lake shape complexity, compactness ratio and form ratio are adopted to researching the evolution law of high water overflows and low water outbursts quantitatively, and clarifying the variation characteristics of landscape morphology with water level gradient in Tonle Sap Lake. The research results have important theoretical significance for the scientific utilization of Tonle Sap Lake water resources and the protection of the lake ecosystem.展开更多
文摘In order to contribute to a better understanding of the biodiversity of local chicken populations, this study focused on the description of the essential qualitative parameters in the phenotypic characterization of local species. Conducted in 6 localities in the Far North Region of Cameroon (Doukoula, Yagoua, Guidiguis, Dziguilao, Maroua 3rd and Méri), a population of 240 local chickens, including 172 hens and 68 cocks were characterized in family farms. The choice of localities was made on the basis of their strong potential local chicken flocks in the region. To this end, each animal was the subject of a qualitative description based on the identification of the sex of the animal, the description of the colorations of the plumage and extremities, and the description of the types of format, plumage and crests. All observations were made with the naked eye and in daylight and then photographed. The main results show that the plumage colors are multiple and dominated by the White-Pied-Black (21.7%) and the Mille-fleur (20.8%);the wattles are dominated by the red (60%) and the pink (35.4%);the tarsi are dominated by the white (43.8%) and the black (32.08%);the white skin (92.5%) dominates over the pink skin (7.5%);the “Smooth-uniform” feather is dominant (97.08%), followed by the smooth-crested type (2.08%) and the fries type (0.82%);the medium size of the chickens is dominant (86.66%), followed by the dwarf size (9.58%) and the large size (3.75%). The results of this study demonstrate that there is a strong phenotypic diversity within the local chicken population. This diversity can serve as a basis for the development of selection, conservation and genetic potential improvement programs based on rational exploitation of the local chicken.
基金the Young Investigator Group“Artificial Intelligence for Probabilistic Weather Forecasting”funded by the Vector Stiftungfunding from the Federal Ministry of Education and Research(BMBF)and the Baden-Württemberg Ministry of Science as part of the Excellence Strategy of the German Federal and State Governments。
文摘Weather forecasts from numerical weather prediction models play a central role in solar energy forecasting,where a cascade of physics-based models is used in a model chain approach to convert forecasts of solar irradiance to solar power production.Ensemble simulations from such weather models aim to quantify uncertainty in the future development of the weather,and can be used to propagate this uncertainty through the model chain to generate probabilistic solar energy predictions.However,ensemble prediction systems are known to exhibit systematic errors,and thus require post-processing to obtain accurate and reliable probabilistic forecasts.The overarching aim of our study is to systematically evaluate different strategies to apply post-processing in model chain approaches with a specific focus on solar energy:not applying any post-processing at all;post-processing only the irradiance predictions before the conversion;post-processing only the solar power predictions obtained from the model chain;or applying post-processing in both steps.In a case study based on a benchmark dataset for the Jacumba solar plant in the U.S.,we develop statistical and machine learning methods for postprocessing ensemble predictions of global horizontal irradiance(GHI)and solar power generation.Further,we propose a neural-network-based model for direct solar power forecasting that bypasses the model chain.Our results indicate that postprocessing substantially improves the solar power generation forecasts,in particular when post-processing is applied to the power predictions.The machine learning methods for post-processing slightly outperform the statistical methods,and the direct forecasting approach performs comparably to the post-processing strategies.
基金funded by the National Natural Science Foundation of China(Grant No.31970232)the National Key Basic Research Program of China(Grant No.2014CB954100).
文摘Dryopteridaceae are the largest fern family and include nearly 20%of extant fern diversity,with 24 currently recognized genera.Recognition and delineation of genera within this family have varied greatly.The three-subfamily classification of Dryopteridaceae was based primarily on molecular phylogenetic relationships but lacked morphological evidence,and the phylogenetic relationships of the subfamilies and genera of Dryopteridaceae are only partially resolved.A comprehensive and robust phylogeny is urgently needed.The heterogeneous morphology of the current members of Dryopteridaceae makes the family and its subfamilies difficult to define by single morphological characteristics or even character combinations.We carried out phylogenetic analyses to reconstruct a highly supported phylogeny of Dryopteridaceae.Our analyses recovered 24 strongly supported clades grouped into seven major clades of Dryopteridaceae.Seven morphological characters including habit,rhizome shape,frond morphology,rachis-costae architecture,appendages on stipe base and lamina,and soral arrangement were found to be informative for identifying different major clades and clades in Dryopteridaceae.Based on phylogenetic reconstruction and morphological analysis,we presented an updated infra-familial classification of Dryopteridaceae with seven subfamilies and 24 genera including four newly proposed subfamilies(Ctenitidoideae,Lastreopsidoideae,Pleocnemioideae,and Polystichopsidoideae).Morphological character combinations of each subfamily are summarized,and a key is provided.Most genera sensu PPG I are recognized,with Stigmatopetris reclassified into Dryopteridoideae and Arthrobotrya considered a synonym of Teratophyllum.A new genus Pseudarachniodes is introduced.This revised classification will serve as a foundational framework for future investigations on taxonomy,biogeography,and diversification of the most species-rich Dryopteridaceae in ferns.
基金supported by the National Key Research and Development Program of China(2023YFD2301500).
文摘A morphology-based growth stage system should describe the growth and development of a crop and thereby help farmers and agronomists in formulating reasonable managementmeasures conducive to the development of marketable products.However,existing growth stage systems for soybean are either based on plant growth or covered particular phases of flower or pod development,making it difficult to use for tracking the entire growth period of individual flowers and pods.Therefore,the first flower and pod,located at the base of the primary raceme in the eighth trifoliate node of the main stem,were chosen to illustrate growth dynamics during the full reproductive period.The size and fresh weight of the primary raceme in the eighth trifoliate leaf axil,the first flower and pod,the pistils in the first flower,and seeds in the first pod were examined,and the growth of these organs was depicted.Integrating the morphological characteristics and growth features of flowers and pods,as well as existing growth stage systems,the growth and development were delineated in 13 stages.In detail,we classified the flower phase based on the relative positions of floral components,inspired by the ratio of bract to flower used for staging,refined the lag phase proposed previously,retained the use of pod length to define the early pod phase,and innovatively described the late pod phase by the seed appearance.The developmental events in each stage of flower and pod were distinctive and closely connected to the corresponding morphology.Taken together,a more detailed growth stage system for describing individual flowers and pods in soybean was established.This system will serve as a valuable research tool for describing the development,gene expression,and cellular metabolism associated with the formation of flowers,pods,and seeds.
基金supported by the National Natural Science Foundation of China (32071544, 32271737)the Interdisciplinary Innovation Team of the Chinese Academy of Sciences (CAS) “Light of West China” Program (xbzg-zdsys-202207)。
文摘Understanding the ecogeographic mechanisms driving morphological variation is pivotal in biogeographic studies. However, patterns and determinants of such variation remain contentious, particularly in amphibians. Although several hypotheses have been formulated and investigated in amphibians, their validity remains disputed with mixed support. Using the Sichuan spiny frog(Nanorana sichuanensis) as an indicator, we investigated the morphological variations across geographic and environmental gradients to explore the underlying ecogeographic mechanisms. We found that both the body size and limb characteristics of N.sichuanensis were not significantly related to latitude or elevation, suggesting that it did not follow Bergmann's or Allen's rules. Eye diameter decreased linearly with increasing elevation and latitude, whereas snout length increased with increasing elevation and latitude. Heat balance, endurance, seasonality, water availability, and primary productivity collectively explain body size variation. Hierarchical partitioning identified primary productivity and thermal excursion as the most influential factors, explaining significant variability in body size and other morphological features. Specifically, primary productivity accounted for 52.40% of the variation in body size, whereas thermal excursion had the greatest impact on eye diameter(36.23%) and snout length(72.17%). Based on body size and dimensionally reduced morphological features, our results identified ecogeographic patterns, assessed the validity of different hypotheses, and examined how environmental factors influence these morphological variations. More generally, our study offers comprehensive insights into the ecogeographic variation observed in mountain amphibians,provides a critical evaluation of existing ecogeographic hypotheses, and infers possible morphological adaptations in response to environmental change.
基金supported by the National KeyR&DProgramof China(2022YFF0710500)the National Natural Science Foundation of China(32172853 and 32373013)the Central Public-interest Scientific Institution Basal Research Fund,China(1610302022001).
文摘The Salmonella pathogenicity islands(SPIs) play crucial roles in the progression of Salmonella infection. In this study, we constructed an improved λ Red homologous recombination system to prepare single and triple deletion mutants of 3 prominent SPIs(SPI-1, 2, and 3), aiming at the impact of deletion on morphology, carbon source metabolism, adhesion and invasion capacity, in vivo colonization, and immune efficacy in chicks. Our examination revealed that the surface of the single deletion mutants(SM6ΔSPI1, ΔSPI2, and ΔSPI3) exhibited a more rugged texture and appeared to be enveloped in a layer of transparent colloid, whereas the morphology of the triple deletion mutant(SM6ΔSPI1&2&3) remained unaltered when compared to the parent strain. The carbon metabolic spectrum of the SPI mutants underwent profound alterations, with a notable and statistically significant modification observed in 30 out of 95 carbon sources, primarily carbohydrates(17 out of 30). Furthermore, the adhesion capacity of the 4 mutants to Caco-2 cells was significantly reduced when compared to that of the parent strain. Moreover,the invasion capacity of mutants SM6ΔSPI1 and SM6ΔSPI1&2&3 exhibited a substantial decrease, while it was enhanced to varying degrees for SM6ΔSPI3 and SM6ΔSPI2. Importantly, none of the 4 mutants induced any clinical symptoms in the chicks. However, they did transiently colonize the spleen and liver. Notably, the SM6ΔSPI1&2&3mutant was rapidly cleared from both the spleen and liver within 8 days post-infection and no notable pathological changes were observed in the organs. Additionally, when challenged, the mutants immunized groups displayed a significant increase in antibody levels and alterations in the CD3+CD4+ and CD3+CD8+ subpopulations, and the levels of IL-4 and IFN-γ cytokines in the SM6ΔSPI1&2&3 immunized chicken serum surpassed those of other groups.In summary, the successful construction of the 4 SPI mutants lays the groundwork for further exploration into the pathogenic(including metabolic) mechanisms of SPIs and the development of safe and effective live attenuated Salmonella vaccines or carriers.
基金supported by the Second Tibetan Plateau Scientific Expedition and Research Program (STEP,2019QZKK0402)Science and Technology Plan Project of Xizang Autonomous Region to Y.Q. (XZ202201ZY0030G)National Natural Science Foundation of China (32201424 to Z.Y.L.and 31872233 to Y.Q.)。
文摘Assessing the threat status of species in response to global change is critical for biodiversity monitoring and conservation efforts.However,current frameworks,even the IUCN Red List,often neglect critical factors such as genetic diversity and the impacts of climate and land-use changes,hindering effective conservation planning.To address these limitations,we developed an enhanced extinction risk assessment framework using Diploderma lizards as a model.This framework incorporates long-term field surveys,environmental data,and land-use information to predict distributional changes for 10 recently described Diploderma species on the Qinghai-Xizang Plateau,which hold ecological significance but remain underassessed in conservation assessment.By integrating the distribution data and genetically inferred effective population sizes(Ne),we conducted scenario analyses and used a rank-sum approach to calculate Risk ranking scores(RRS)for each species.This approach revealed significant discrepancies with the IUCN Red List assessments.Notably,D.yangi and D.qilin were identified as facing the highest extinction risk.Furthermore,D.vela,D.batangense,D.flaviceps,D.dymondi,D.yulongense,and D.laeviventre,currently classified as“Least Concern”,were found to warrant reclassification as“Vulnerable”due to considerable threat from projected range contractions.Exploring the relationship between morphology and RRS revealed that traits such as snout-vent length and relative tail length could serve as potential predictors of extinction risk,offering preliminary metrics for assessing species vulnerability when comprehensive data are unavailable.This study enhances the precision of extinction risk assessment frameworks and demonstrates their capacity to refine and update risk assessments,especially for lesser-known taxa.
文摘WITHDRAWAL:Zhang,J.J.,Guo,Y.Q.,Qin,Z.Y.,Wei,C.T.,Hu,Q.H.,Vandeginste,V.,Miao,H.Y.,Yao,P.,and Zhang,P.F.,“Predicting Irreducible Water Saturation of Unconventional Reservoirs by Using NMR T2 Spectra:Methods of Morphological Division and Fractal Models”,Acta Geologica Sinica-English Edition(Accepted Article):https://doi.org/10.1111/1755-6724.15094.
基金Supported by the National Natural Science Foundation of China,No.81900533Science and Technology Project of Henan Science and Technology Department,No.232102520032。
文摘Autophagy is a cellular catabolic process characterized by the formation of double-membrane autophagosomes.Transmission electron microscopy is the most rigorous method to clearly visualize autophagic engulfment and degradation.A large number of studies have shown that autophagy is closely related to the digestion,secretion,and regeneration of gastrointestinal(GI)cells.However,the role of autophagy in GI diseases remains controversial.This article focuses on the morphological and biochemical characteristics of autophagy in GI diseases,in order to provide new ideas for their diagnosis and treatment.
基金Supported by the National Natural Science Foundation of China(No.32170205)the Natural Science Foundation of Shanghai(No.21ZR144730)。
文摘In our recent investigations of diatom diversity,we studied three species,namely,Skeletonema costatum,Skeletonema subsalsum,and Skeletonema potamos.Although they have been found frequently in Changjiang(Yangtze)River Basin,their morphological and molecular identification is difficult in taxonomy.Therefore,to integrate morphological and molecular biological approaches,we compared systematically their morphological characters and performed phylogenetic analysis.Twelve strains of Skeletonema were collected and isolated from Shanghai and Jiangsu,China,and their morphological characteristics were examined by light microscopy(LM)and the scanning electron microscopy(SEM).Based on morphological comparison,we determined that S.potamos is easy to distinguish from the other two species.The heavily silicified areolae,undulated or cleft distal ends of terminal fultoportula processes(TFPPs),absence of basal pores of fultoportula processes(FPPs),the rootlike protrusions of FPPs,and no interlocking connection are the stable characteristics that can be used to identify S.potamos.However,there are only two features that can distinguish S.costatum from S.subsalsum,namely the location of terminal rimoportulae(TRPs)and the distal shape of TFPPs.In addition,we amplified and sequenced nine common genetic markers from the strains,from which 101 sequences were obtained,constructed phylogenetic trees based on the nine genes and evaluated that seven genes can be used to identify S.potamos,and revealed that S.subsalsum is the closest known relative of S.costatum,and only ATP synthetase beta-subunit gene(atp B)is able to distinguish them from each other,which strongly support that it is an effective molecular marker for Skeletonema.This work provided a theoretical basis for the taxonomic study of Skeletonema.
文摘Country bean, Lablab purpureus (L.) is considered one of the most important leguminous crops, but their cultivation under drought stress condition encounters challenges. In this study, an experiment has been conducted among 30 genotypes under drought condition to explore morphological diversity of qualitative and quantitative, biochemical, molecular analysis. The study identified significant variations in eight traits among the genotypes examined, with phenotypic variance exceeding genotypic variance, indicating both genetic and environmental influences. High heritability and genetic advance were observed in primary, secondary, and tertiary branch lengths, suggesting these traits are likely controlled by additive gene effects, making them effective targets for selection. Principal component analysis identified three components that made a substantial contribution, accounting for approximately 73.06% of the overall quantitative variations. Among the quantitative traits, the highest coefficient of variation (CV%) has been found in number of flowers (55.05%). While number of primary branches, primary branch length, number of secondary branches, secondary branch length, number of tertiary branches, tertiary branch length has individually more than 20% of CV%. The genotypes have been grouped into three clusters based on quantitative traits. Analysis of protein reveals that the genotypes of DS28 and DS29 have higher protein content than other genotypes. Dehydrogenase responsive genotypes have been found on DS28 and DS29 from the molecular analysis. The results suggest that the genotypes DS28 and DS29 could contribute as genetic resource of high protein content and DREB responsive, and the eight quantitative traits of 30 genotypes could be used for further breeding programme.
文摘In this study, 32 black rice genotypes spanning diverse geographies in India were evaluated at both phenotypic and DNA sequence levels to gain insights into their genetic makeup. Significant variations were observed for 14 agro-morphological traits, showing correlations among several yield-related traits.
基金This study was supported by the National Natural Science Foundation of China,Grant/Award Number:32071779.
文摘In overlapping distribution areas of Sorbus pohuashanensis and S.discolor in North China(Mount Tuoliang,Mount Xiling and Mount Baihua),Sorbus indi-viduals were found with pink fruit,which have never been recorded for the flora of China.Fourteen morphological characters combined with four chloroplast DNA markers and internal transcribed spacer(ITS)were used to analyze the origin of the Sorbus individuals with pink fruits and their relationship to S.pohuashanensis and S.discolor.PCA,SDA and one-way(taxon)ANOVA of morphological characters provided convincing evidence of the hybrid ori-gin of Sorbus individuals with pink fruits based on a novel morphological character and many intermediate characters.Haplotype analysis based on four cpDNA markers showed that either S.pohuashanensis or S.discolor were maternal parents of Sorbus individuals with pink fruits.Incongru-ence of the position of Sorbus individuals with pink fruits between cpDNA and ITS in cluster trees supported by DNA sequence comparative analysis,implying former hybridiza-tion events between S.pohuashanensis and S.discolor.Mul-tiple hybridization events between S.pohuashanensis and S.discolor might have contributed to the generation of Sorbus individuals with pink fruits.This study has provided insights into hybridization between species of the same genus in sympatric areas,which is of great significance for the study of interspecific hybridization.
基金funded by the National Natural Science Foundation of China (No.32161143015)International Partnership Program of Chinese Academy of Sciences (070GJHZ202211FN)+5 种基金the Natural Science Foundation of Yunnan Province (202001AS070016)the"Ten Thousand Talents Program of Yunnan" (Top-notch Young Talents Project,No.YNWR-QNBJ-2018-279)the CAS Interdisciplinary Team of the"Light of West China"programYunnan Revitalization Talent Support Program"Innovation Team"project to CLXthe Iran National Science Foundation to YS (4001651)the open research project of the Germplasm Bank of Wild Species,Kunming Institute of Botany,Chinese Academy of Sciences。
文摘Phlomoides,with 150-170 species,is the second largest and perhaps most taxonomically challenging genus within the subfamily Lamioideae(Lamiaceae).With about 60 species,China is one of three major biodiversity centers of Phlomoides.Although some Phlomoides species from China have been included in previous molecular phylogenetic studies,a robust and broad phylogeny of this lineage has yet to be completed.Moreover,given the myriad new additions to the genus,the existing infrageneric classification needs to be evaluated and revised.Here,we combine molecular and morphological data to investigate relationships within Phlomoides,with a focus on Chinese species.We observed that plastid DNA sequences can resolve relationships within Phlomoides better than nuclear ribosomal internal and external transcribed spacer regions(nrITS and nrETS).Molecular phylogenetic analyses confirm the monophyly of Phlomoides,but most previously defined infrageneric groups are not monophyletic.In addition,morphological analysis demonstrates the significant taxonomic value of eight characters to the genus.Based on our molecular phylogenetic analyses and morphological data,we establish a novel section Notochaete within Phlomoides,and propose three new combinations as well as three new synonyms.This study presents the first molecular phylogenetic analyses of Phlomoides in which taxa representative of the entire genus are included,and highlights the phylogenetic and taxonomic value of several morphological characters from species of Phlomoides from China.Our study suggests that a taxonomic revision and reclassification for the entire genus is necessary in the future.
基金the Space Application Center, Ahmedabad (ISRO) for providing field support under “Integrated studies of Himalayan Cryosphere” programthe Glaciology Group, Jawaharlal Nehru University for providing necessary support for this research+1 种基金the grants from SERB (CRG/2020/004877) and MOES/16/19/2017-RDEAS projectsthe support from ISRO/RES/4/690/21-22 project
文摘Glacier inventories serve as critical baseline data for understanding the impacts of climate change on glaciers.The present study maps the outlines of glaciers in the Chandra-Bhaga Basin(western Himalaya)for the years 1993,2000,2010,and 2019 using Landsat Thematic Mapper(TM),Enhanced Thematic Mapper(ETM),and Operational Land Imager(OLI)datasets.A total of 251 glaciers,each having an area above 0.5 km^(2),were identified,which include 216 clean-ice and 35 debris-covered glaciers.Area changes are estimated for three periods:1993-2000,2000-2010,and 2010-2019.The total glacierized area was 996±62 km^(2) in 1993,which decreased to 973±70 km^(2) in 2019.The mean rate of glacier area loss was higher in the recent decade(2010-2019),at 0.036 km^(2),compared to previous decades(0.029 km^(2) in 2000-2010 and 0.025 km^(2) in 1993-2000).Supraglacial debris cover changes are also mapped over the period of 1993 and 2019.It is found that the supraglacial debris cover increased by 14.12±2.54 km^(2)(15.2%)during 1993-2019.Extensive field surveys on Chhota Shigri,Panchi II,Patsio,Hamtah,Mulkila,and Yoche Lungpa glaciers were carried out to validate the glacier outlines and supraglacial debris cover estimated using satellite datasets.Controls of various morphological parameters on retreat were also analyzed.It is observed that small,clean ice,south oriented glaciers,and glaciers with proglacial lakes are losing area at faster rates than other glaciers in the basin.
基金the National Natural Science Foundation of China(62205368)the Key Research and Development Program of Jiangsu Province(BE2020664).
文摘Light-sheet fluorescence microscopy(LSFM)has been widely used to image the three-dimensional(3D)structures and functions of various millimeter-size bio-specimen such as zebrafish.However,the sample adsorption and scattering cause shading of the light-sheet illumination,preventing the even 3D image of thick samples.Herein,we report a continuous-rotational light-sheet microscope(CR-LSM)that enables simultaneous 3D bright-field and fluorescence imaging.With a high-accuracy rotational stage,CR-LSM records the outline projections and the fluorescent images of the sample at multiple rotation angles.Then,3D morphology and fluorescent structure were reconstructed with a developed algorithm.Using CR-LSM,zebrafish’s whole-fish contour and blood vessel structures were obtained simultaneously.
基金supported by the National Natural Science Foundation of China (Grant Nos. 11874140 and 12174085)Basic Science (Natural Science) Research Project for the Universities of Jiangsu Province (Grant No. 23KJD140002)the Natural Science Foundation of Nantong (Grant No. JC2023081)。
文摘A fractal geometric boundary with natural wall features is introduced into a hybrid lattice-Boltzmann-method(LBM)multiphase model. The physical model of cavitation bubble collapse near the irregular geometric wall is established to study the thermodynamic characteristics of the bubble collapse. Due to the lack of periodicity, symmetry, spatial uniformity and obvious correlation in the LBM simulation of the bubble collapse near the fractal wall, the morphological analysis based on Minkowski functional is introduced into the thermodynamic investigation of cavitation bubble so as to analyze and obtain the effective information. The results show that the Minkowski functional method can employed to study the temperature information in complex physical fields hierarchically and quantitatively. The high/low temperature region of the cavitation flow is explored, and thermal effect between irregular and fractal geometric wall and cavitation bubble can be revealed. It illustrates that LBM and morphological analysis complement each other, and morphological analysis can also be used as an optional and potential tool in research field of complex multiphase flows.
文摘This study evaluated the variation in yellow root cassava (Manihot esculentus Crantz) genotypes and phenotypic relationship for selected postharvest and morphological traits. The trial was established at the Njala Agricultural Research Centre experimental site, Njala, during 2017/2018 cropping season in a randomized complete block design with three replications. Findings showed that the higher the total carotene content (TCC) in yellow flesh cassava genotypes, the longer the rate of postharvest physiological deterioration (PPD). Genotypes TR-0051-TCC/17 and TR-0012-TCC/17 recorded higher TCC (18.9 µg/g and 13.6 µg/g) and longer rate of PPD (4.29 and 3.14), respectively. Genotypes TR-0051-TCC/17, TR-0016-TCC/17, TR-0028-TCC/17, TR-0012-TCC/17 and TR-0020-TCC/17 had the highest TCC values of 18.9 µg/g, 16.09 µg/g, 14.72 µg/g, 13.6 µg/g and 11.23 µg/g with corresponding higher color chart values of 6, 6, 6, 5, and 6, respectively. This suggests the direct dependence of TCC on the root parenchyma color intensity in yellow flesh cassava genotypes. Findings also show a direct relationship between morphological and postharvest traits in yellow flesh cassava genotypes that could be exploited for the genetic improvement of cassava for increased shelf life, nutrition and related quality traits, as well as conservation and utilization of the crop.
基金Supported by the National Natural Science Foundation of China(No.81400428)Self-selected Projects of Shanghai Children’s Hospital(No.2020R124)Shanghai Children’s Hospital Hospital-level Project Clinical Research Cultivation Special Focus Project(No.2021YLYZ03).
文摘AIM:To quantitatively measure ocular morphological parameters of guinea pig with Python technology.METHODS:Thirty-six eyeballs of eighteen 3-weekold guinea pigs were measured with keratometer and photographed to obtain the horizontal,coronal,and sagittal planes respectively.The corresponding photo pixels-actual length ratio was acquired by a proportional scale.The edge coordinates were identified artificially by ginput function.Circle and conic curve fitting were applied to fit the contour of the eyeball in the sagittal,coronal and horizontal view.The curvature,curvature radius,eccentricity,tilt angle,corneal diameter,and binocular separation angle were calculated according to the geometric principles.Next,the eyeballs were removed,canny edge detection was applied to identify the contour of eyeball in vitro.The results were compared between in vivo and in vitro.RESULTS:Regarding the corneal curvature and curvature radius on the horizontal and sagittal planes,no significant differences were observed among results in vivo,in vitro,and the keratometer.The horizontal and vertical binocular separation angles were 130.6°±6.39°and 129.8°±9.58°respectively.For the corneal curvature radius and eccentricity in vivo,significant differences were observed between horizontal and vertical planes.CONCLUSION:The Graphical interface window of Python makes up the deficiency of edge detection,which requires too much definition in Matlab.There are significant differences between guinea pig and human beings,such as exotropic eye position,oblique oval eyeball,and obvious discrepancy of binoculus.This study helps evaluate objectively the ocular morphological parameters of small experimental animals in emmetropization research.
文摘Lake is an important part of the natural ecosystem, and its morphological characteristics reflect the capacity of lake regulation and storage, the strength of material migration, and the characteristics of shoreline development. In most existing studies, remote sensing images are used to quantify the morphological characteristics of lakes. However, the extraction accuracy of lake water is greatly affected by cloud cover and vegetation cover, and the inversion accuracy of lake elevation data is poor, which cannot accurately describe the response relationship of lake landscape morphology with water level change. Therefore, this paper takes Tonle Sap Lake as the research object, which is the largest natural freshwater lake in Southeast Asia. DEM is constructed based on high-resolution measured topographic data, and morphological indicators such as lake area, lake shoreline length, perimeter area ratio, longest axis length, maximum width, shoreline development index, lake shape complexity, compactness ratio and form ratio are adopted to researching the evolution law of high water overflows and low water outbursts quantitatively, and clarifying the variation characteristics of landscape morphology with water level gradient in Tonle Sap Lake. The research results have important theoretical significance for the scientific utilization of Tonle Sap Lake water resources and the protection of the lake ecosystem.
