The COVID-19 pandemic has not only affected the world in terms of their physical health but has also been a strain on our mental well-being.Individuals who have showcased the tendency to bounce back from this situatio...The COVID-19 pandemic has not only affected the world in terms of their physical health but has also been a strain on our mental well-being.Individuals who have showcased the tendency to bounce back from this situation have been real survivors of this pandemic.With this background in mind,this research aimed to study the gender differences and age differences in stress resilience.The Stress Resiliency Profile by Thomas and Tymon(1992),Jr.was used for this purpose.An equal number of males and females(n=60;N=120)were evaluated for the study.To assess the age differences,the participants were divided into two age groups:15-22 years and 22-30 years of age.There were an equal number of individuals in each group(n=60,N=120).The subjects were assessed on the three dimensions of Stress Resiliency Profile-Necessitating,Skill Recognition and Deficiency Focusing.Results indicated significant differences in the Deficiency Focusing dimension among males and females.In the age difference evaluation,significant differences were found in the Skill Recognition dimension.The findings have been discussed within the framework of previous research.The current research findings have significant implications for the study of stress and resilience.展开更多
Stressors, such as work overload, role conflicts, lack of time, lack of self-care, poor job-related interpersonal relationships, feeling powerless to provide quality care, struggling with competing demands,death, conf...Stressors, such as work overload, role conflicts, lack of time, lack of self-care, poor job-related interpersonal relationships, feeling powerless to provide quality care, struggling with competing demands,death, conflict with doctors, peers and supervisors, and inadequate emotional preparation, can be negative, stressful, traumatic, or difficult situations or episodes of hardship for nurses. Situations or episodes of these types can also cause both physical and mental problems, such as fatigue, irritability,lack of concentration, unhappiness, depressive sensation, depersonalization, and emotional exhaustion.Despite all of these challenges, resilience enables nurses to cope with their work environment and to maintain healthy and stable psychological functioning. The aim of this review is to describe the concept of resilience, explain characteristics with which it is related and outline interventions to increase the resilience of nurses.展开更多
RNA-binding proteins(RBPs)accompany RNA from synthesis to decay,mediating every aspect of RNA metabolism and impacting diverse cellular and developmental processes in eukaryotes.Many RBPs undergo phase separation alon...RNA-binding proteins(RBPs)accompany RNA from synthesis to decay,mediating every aspect of RNA metabolism and impacting diverse cellular and developmental processes in eukaryotes.Many RBPs undergo phase separation along with their bound RNA to form and function in dynamic membraneless biomolecular condensates for spatiotemporal coordination or regulation of RNA metabolism.Increasing evidence suggests that phase-separating RBPs with RNA-binding domains and intrinsically disordered regions play important roles in plant development and stress adaptation.Here,we summarize the current knowledge about how dynamic partitioning of RBPs into condensates controls plant development and enables sensing of experimental changes to confer growth plasticity under stress conditions,with a focus on the dynamics and functional mechanisms of RBP-rich nuclear condensates and cytoplasmic granules in mediating RNA metabolism.We also discuss roles of multiple factors,such as environmental signals,protein modifications,and N6-methyladenosine RNA methylation,in modulating the phase separation behaviors of RBPs,and highlight the prospects and challenges for future research on phase-separating RBPs in crops.展开更多
Neurogenesis decline in hippocampal dentate gyrus(DG)participates in stress-induced depressive-like behaviors,but the underlying mechanism remains poorly understood.Here,we observed low-expression of NOD-like receptor...Neurogenesis decline in hippocampal dentate gyrus(DG)participates in stress-induced depressive-like behaviors,but the underlying mechanism remains poorly understood.Here,we observed low-expression of NOD-like receptor family pyrin domain containing 6(NLRP6)in hippocampus of stress-stimulated mice,being consistent with high corticosterone level.NLRP6 was found to be abundantly expressed in neural stem cells(NSCs)of DG.Both Nlrp6 knockout(Nlrp6^(-/-))and NSCconditional Nlrp6 knockout(Nlrp6CKO)mice were susceptible to stress,being more likely to develop depressive-like behaviors.Interestingly,NLRP6 was required for NSC proliferation in sustaining hippocampal neurogenesis and reinforcing stress resilience during growing up.Nlrp6 deficiency promoted esophageal cancer-related gene 4(ECRG4)expression and caused mitochondrial dysfunction.Corticosterone as a stress factor significantly down-regulated NLRP6 expression,damaged mitochondrial function and suppressed cell proliferation in NSCs,which were blocked by Nlrp6 overexpression.ECRG4 knockdown reversed corticosterone-induced NSC mitochondrial function and cell proliferation disorders.Pioglitazone,a well-known clinical drug,up-regulated NLRP6 expression to inhibit ECRG4 expression in its protection against corticosterone-induced NSC mitochondrial dysfunction and proliferation restriction.In conclusion,this study demonstrates that NLRP6 is essential to maintain mitochondrial homeostasis and proliferation in NSCs,and identifies NLRP6 as a promising therapeutic target for hippocampal neurogenesis decline linked to depression.展开更多
Modern agricultural systems are directly threatened by global climate change and the resulting freshwater crisis.A considerable challenge in the coming years will be to develop crops that can cope with the conse-quenc...Modern agricultural systems are directly threatened by global climate change and the resulting freshwater crisis.A considerable challenge in the coming years will be to develop crops that can cope with the conse-quences of declining freshwater resources and changing temperatures.One approach to meeting this chal-lenge may lie in our understanding of plant photosynthetic adaptations and water use efciency.Plants from various taxa have evolved crassulacean acid metabolism(CAM),a water-conserving adaptation of photosynthetic carbon dioxidexation that enables plants to thrive under semi-arid or seasonally drought-prone conditions.Although past research on CAM has led to a better understanding of the inner workings of plant resilience and adaptation to stress,successful introduction of this pathway into C3 or C4 plants has not been reported.The recent revolution in molecular,systems,and synthetic biology,as well as innovations in high-throughput data generation and mining,creates new opportunities to uncover the minimum genetic tool kit required to introduce CAM traits into drought-sensitive crops.Here,we pro-pose four complementary research avenues to uncover this tool kit.First,genomes and computational methods should be used to improve understanding of the nature of variations that drive CAM evolution.Second,single-cell’omics technologies offer the possibility for in-depth characterization of the mecha-nisms that trigger environmentally controlled CAM induction.Third,the rapid increase in new’omics data enables a comprehensive,multimodal exploration of CAM.Finally,the expansion of functional geno-mics methods is paving the way for integration of CAM into farming systems.展开更多
文摘The COVID-19 pandemic has not only affected the world in terms of their physical health but has also been a strain on our mental well-being.Individuals who have showcased the tendency to bounce back from this situation have been real survivors of this pandemic.With this background in mind,this research aimed to study the gender differences and age differences in stress resilience.The Stress Resiliency Profile by Thomas and Tymon(1992),Jr.was used for this purpose.An equal number of males and females(n=60;N=120)were evaluated for the study.To assess the age differences,the participants were divided into two age groups:15-22 years and 22-30 years of age.There were an equal number of individuals in each group(n=60,N=120).The subjects were assessed on the three dimensions of Stress Resiliency Profile-Necessitating,Skill Recognition and Deficiency Focusing.Results indicated significant differences in the Deficiency Focusing dimension among males and females.In the age difference evaluation,significant differences were found in the Skill Recognition dimension.The findings have been discussed within the framework of previous research.The current research findings have significant implications for the study of stress and resilience.
文摘Stressors, such as work overload, role conflicts, lack of time, lack of self-care, poor job-related interpersonal relationships, feeling powerless to provide quality care, struggling with competing demands,death, conflict with doctors, peers and supervisors, and inadequate emotional preparation, can be negative, stressful, traumatic, or difficult situations or episodes of hardship for nurses. Situations or episodes of these types can also cause both physical and mental problems, such as fatigue, irritability,lack of concentration, unhappiness, depressive sensation, depersonalization, and emotional exhaustion.Despite all of these challenges, resilience enables nurses to cope with their work environment and to maintain healthy and stable psychological functioning. The aim of this review is to describe the concept of resilience, explain characteristics with which it is related and outline interventions to increase the resilience of nurses.
基金supported by the National Research Foundation Competitive Research Programme(NRF-CRP22-2019-0001)the intramural funding from Temasek Life Sciences Laboratory。
文摘RNA-binding proteins(RBPs)accompany RNA from synthesis to decay,mediating every aspect of RNA metabolism and impacting diverse cellular and developmental processes in eukaryotes.Many RBPs undergo phase separation along with their bound RNA to form and function in dynamic membraneless biomolecular condensates for spatiotemporal coordination or regulation of RNA metabolism.Increasing evidence suggests that phase-separating RBPs with RNA-binding domains and intrinsically disordered regions play important roles in plant development and stress adaptation.Here,we summarize the current knowledge about how dynamic partitioning of RBPs into condensates controls plant development and enables sensing of experimental changes to confer growth plasticity under stress conditions,with a focus on the dynamics and functional mechanisms of RBP-rich nuclear condensates and cytoplasmic granules in mediating RNA metabolism.We also discuss roles of multiple factors,such as environmental signals,protein modifications,and N6-methyladenosine RNA methylation,in modulating the phase separation behaviors of RBPs,and highlight the prospects and challenges for future research on phase-separating RBPs in crops.
基金National Key R&D Program of China(2022YFC3500303)National Natural Science Foundation of China(81991522)partly by the Open Project of State Key Laboratory of Natural Medicine,No.SKLNMKF202204,China。
文摘Neurogenesis decline in hippocampal dentate gyrus(DG)participates in stress-induced depressive-like behaviors,but the underlying mechanism remains poorly understood.Here,we observed low-expression of NOD-like receptor family pyrin domain containing 6(NLRP6)in hippocampus of stress-stimulated mice,being consistent with high corticosterone level.NLRP6 was found to be abundantly expressed in neural stem cells(NSCs)of DG.Both Nlrp6 knockout(Nlrp6^(-/-))and NSCconditional Nlrp6 knockout(Nlrp6CKO)mice were susceptible to stress,being more likely to develop depressive-like behaviors.Interestingly,NLRP6 was required for NSC proliferation in sustaining hippocampal neurogenesis and reinforcing stress resilience during growing up.Nlrp6 deficiency promoted esophageal cancer-related gene 4(ECRG4)expression and caused mitochondrial dysfunction.Corticosterone as a stress factor significantly down-regulated NLRP6 expression,damaged mitochondrial function and suppressed cell proliferation in NSCs,which were blocked by Nlrp6 overexpression.ECRG4 knockdown reversed corticosterone-induced NSC mitochondrial function and cell proliferation disorders.Pioglitazone,a well-known clinical drug,up-regulated NLRP6 expression to inhibit ECRG4 expression in its protection against corticosterone-induced NSC mitochondrial dysfunction and proliferation restriction.In conclusion,this study demonstrates that NLRP6 is essential to maintain mitochondrial homeostasis and proliferation in NSCs,and identifies NLRP6 as a promising therapeutic target for hippocampal neurogenesis decline linked to depression.
文摘Modern agricultural systems are directly threatened by global climate change and the resulting freshwater crisis.A considerable challenge in the coming years will be to develop crops that can cope with the conse-quences of declining freshwater resources and changing temperatures.One approach to meeting this chal-lenge may lie in our understanding of plant photosynthetic adaptations and water use efciency.Plants from various taxa have evolved crassulacean acid metabolism(CAM),a water-conserving adaptation of photosynthetic carbon dioxidexation that enables plants to thrive under semi-arid or seasonally drought-prone conditions.Although past research on CAM has led to a better understanding of the inner workings of plant resilience and adaptation to stress,successful introduction of this pathway into C3 or C4 plants has not been reported.The recent revolution in molecular,systems,and synthetic biology,as well as innovations in high-throughput data generation and mining,creates new opportunities to uncover the minimum genetic tool kit required to introduce CAM traits into drought-sensitive crops.Here,we pro-pose four complementary research avenues to uncover this tool kit.First,genomes and computational methods should be used to improve understanding of the nature of variations that drive CAM evolution.Second,single-cell’omics technologies offer the possibility for in-depth characterization of the mecha-nisms that trigger environmentally controlled CAM induction.Third,the rapid increase in new’omics data enables a comprehensive,multimodal exploration of CAM.Finally,the expansion of functional geno-mics methods is paving the way for integration of CAM into farming systems.
