Objective:To explore the prognosis-predictive influence of human epidermal growth factor receptor 2(HER2)-low status in breast cancer patients after neoadjuvant therapy(NAT).Methods:Consecutive patients with invasive ...Objective:To explore the prognosis-predictive influence of human epidermal growth factor receptor 2(HER2)-low status in breast cancer patients after neoadjuvant therapy(NAT).Methods:Consecutive patients with invasive breast cancer who underwent NAT and surgery from January 2009 to December 2020 at multiple centers were included.A modified CPS+EG scoring system that integrates HER2-low status,CPS+EGH_(low)was developed.Multiple scoring systems were compared via receiver operating characteristic curves with the area under curve(AUC),the Akaike information criterion,the C-index,and calibration curves.Results:A total of 2,141 patients were included:1,074,640,and 427 patients in the training,internal validation,and external validation groups,respectively.HER2-low patients had a significantly better breast cancer-specific survival(BCSS,P=0.008)and recurrence-free interval(RFI,P=0.030)compared to HER2-zero patients(P=0.038)but inferior outcomes than HER2-amplified ones(BCSS,P=0.002;RFI,P<0.001).The CPS+EGH_(low)(AUC:0.846,0.817,0.901)could stratify patients according to BCSS in training,internal validation,and external validation group,respectively,overperforming pathological stage(PS)(AUC:0.746,0.779,0.754),CPS+EG(AUC:0.771,0.752,0.748),and Neo-Bioscore(AUC:0.783,0.777,0.786,all P<0.05).Conclusions:HER2-low status showed a significant prognostic value in breast cancer patients after NAT.The CPS+EGH_(low)model significantly outperformed PS,CPS+EG,and Neo-Bioscore in clinical outcome prediction,which may guide further therapy targeting HER2-low.展开更多
Paeonia suffruticosa Andr.is an endemic shrub flower in China with 2n=10.This study used 228 cultivars from four populations,i.e.,Jiangnan,Japan,Northwest,and Zhongyuan,as materials to explore the genetic diversity le...Paeonia suffruticosa Andr.is an endemic shrub flower in China with 2n=10.This study used 228 cultivars from four populations,i.e.,Jiangnan,Japan,Northwest,and Zhongyuan,as materials to explore the genetic diversity levels among different populations of tree peony varieties.The results showed that 34 bands were amplified using five pairs of cp SSR primers,with an average of 6.8 bands per primer pair.The average number of different alleles(N_(a)),effective alleles(N_(e)),Shannon's information index(I),diversity(H),and polymorphic information content(PIC)were 3.600,2.053,0.708,0.433,and 0.388,respectively.The PIC value was between 0.250 and 0.500,indicating a moderate level of polymorphism for the five cp SSR primer pairs.The genetic diversity levels of peony cultivars varied among different populations,with the Northwest population showing relatively lower levels(I=0.590,H=0.289,and PIC=0.263).A total of 52 haplotypes were identified in the four examined populations,and the number of haplotypes per population ranged from 11 to 22.Forty-four private haplotypes were detected across populations,and the Northwest population exhibiting the highest count of private haplotypes with 17.The mean number of effective number of haplotypes(N_(eh)),haplotypic richness(R_(h)),and diversity(H)were 8.351,6.824,and 0.893,respectively.Analysis of molecular variance indicated that genetic variation within tree peony germplasm was greater than that between germplasm resources,and the main variation was found within individuals of peony germplasm.Cluster analysis,principal coordinate analysis,and genetic structure analysis classified tree peonies from different origins into two groups,indicating a certain degree of genetic differentiation among these four tree peony cultivation groups.This study provides a theoretical basis for the exploration,utilization,and conservation of peony germplasm resources,as well as for research on the breeding of excellent varieties.展开更多
[ Objective] The aim of this study was to establish the optimum cpSSR-PCR system for Jatropha curcas Linn. [ Method] cpSSR-PCR amplification system for Jatropha curcas Linn influenced by five factors including Taq DNA...[ Objective] The aim of this study was to establish the optimum cpSSR-PCR system for Jatropha curcas Linn. [ Method] cpSSR-PCR amplification system for Jatropha curcas Linn influenced by five factors including Taq DNA polymerase, Mg^2＋ , DNA template, dNTP and primer were optimized from several levels. [ Result] The optimum concentration of 20 μl reaction system was 10 × Buffer, 2.00 mmol/L Mg^2＋ , 2 U/μl Taq DNA polymerase, 0.2 mmol/L dNTP, 0.2 μmol/L primer and 35 ng/μl DNA template. [ Conclusion] The optimum annealing temperature for cpSSR-PCR reaction system is 52 ℃, and the cpSSR reaction system is steady and reproducible.展开更多
三钢6.25 m 3号焦炉采用炭化室压力稳定系统,简称CPS系统,该应用可以实现焦炉无烟装煤以及结焦过程中各炭化室的压力稳定。CPS系统依据装煤、出焦信号,自动完成相应操作,提高了焦炉生产的自动化水平。在投产前期,CPS系统经常出现问题,...三钢6.25 m 3号焦炉采用炭化室压力稳定系统,简称CPS系统,该应用可以实现焦炉无烟装煤以及结焦过程中各炭化室的压力稳定。CPS系统依据装煤、出焦信号,自动完成相应操作,提高了焦炉生产的自动化水平。在投产前期,CPS系统经常出现问题,但通过不断摸索、研究,并针对性地进行改进处理后,相关故障基本上得以消除。展开更多
The advent of Industry 5.0 marks a transformative era where Cyber-Physical Systems(CPSs)seamlessly integrate physical processes with advanced digital technologies.However,as industries become increasingly interconnect...The advent of Industry 5.0 marks a transformative era where Cyber-Physical Systems(CPSs)seamlessly integrate physical processes with advanced digital technologies.However,as industries become increasingly interconnected and reliant on smart digital technologies,the intersection of physical and cyber domains introduces novel security considerations,endangering the entire industrial ecosystem.The transition towards a more cooperative setting,including humans and machines in Industry 5.0,together with the growing intricacy and interconnection of CPSs,presents distinct and diverse security and privacy challenges.In this regard,this study provides a comprehensive review of security and privacy concerns pertaining to CPSs in the context of Industry 5.0.The review commences by providing an outline of the role of CPSs in Industry 5.0 and then proceeds to conduct a thorough review of the different security risks associated with CPSs in the context of Industry 5.0.Afterward,the study also presents the privacy implications inherent in these systems,particularly in light of the massive data collection and processing required.In addition,the paper delineates potential avenues for future research and provides countermeasures to surmount these challenges.Overall,the study underscores the imperative of adopting comprehensive security and privacy strategies within the context of Industry 5.0.展开更多
In this paper, we study stealthy cyber-attacks on actuators of cyber-physical systems(CPS), namely zero dynamics and controllable attacks. In particular, under certain assumptions, we investigate and propose condition...In this paper, we study stealthy cyber-attacks on actuators of cyber-physical systems(CPS), namely zero dynamics and controllable attacks. In particular, under certain assumptions, we investigate and propose conditions under which one can execute zero dynamics and controllable attacks in the CPS. The above conditions are derived based on the Markov parameters of the CPS and elements of the system observability matrix. Consequently, in addition to outlining the number of required actuators to be attacked, these conditions provide one with the minimum system knowledge needed to perform zero dynamics and controllable cyber-attacks. As a countermeasure against the above stealthy cyber-attacks, we develop a dynamic coding scheme that increases the minimum number of the CPS required actuators to carry out zero dynamics and controllable cyber-attacks to its maximum possible value. It is shown that if at least one secure input channel exists, the proposed dynamic coding scheme can prevent adversaries from executing the zero dynamics and controllable attacks even if they have complete knowledge of the coding system. Finally, two illustrative numerical case studies are provided to demonstrate the effectiveness and capabilities of our derived conditions and proposed methodologies.展开更多
基金supported by the National Natural Science Foundation of China(No.82072937 and 82072897)Interdisciplinary Program of Shanghai Jiao Tong University(No.YG2024QNB05)。
文摘Objective:To explore the prognosis-predictive influence of human epidermal growth factor receptor 2(HER2)-low status in breast cancer patients after neoadjuvant therapy(NAT).Methods:Consecutive patients with invasive breast cancer who underwent NAT and surgery from January 2009 to December 2020 at multiple centers were included.A modified CPS+EG scoring system that integrates HER2-low status,CPS+EGH_(low)was developed.Multiple scoring systems were compared via receiver operating characteristic curves with the area under curve(AUC),the Akaike information criterion,the C-index,and calibration curves.Results:A total of 2,141 patients were included:1,074,640,and 427 patients in the training,internal validation,and external validation groups,respectively.HER2-low patients had a significantly better breast cancer-specific survival(BCSS,P=0.008)and recurrence-free interval(RFI,P=0.030)compared to HER2-zero patients(P=0.038)but inferior outcomes than HER2-amplified ones(BCSS,P=0.002;RFI,P<0.001).The CPS+EGH_(low)(AUC:0.846,0.817,0.901)could stratify patients according to BCSS in training,internal validation,and external validation group,respectively,overperforming pathological stage(PS)(AUC:0.746,0.779,0.754),CPS+EG(AUC:0.771,0.752,0.748),and Neo-Bioscore(AUC:0.783,0.777,0.786,all P<0.05).Conclusions:HER2-low status showed a significant prognostic value in breast cancer patients after NAT.The CPS+EGH_(low)model significantly outperformed PS,CPS+EG,and Neo-Bioscore in clinical outcome prediction,which may guide further therapy targeting HER2-low.
基金supported by Innovation Scientists and Technicians Troop Construction Projects of Henan Province(Grant No.212101510003)the Central Plains Scholar Workstation Project(Grant No.224400510002)+1 种基金the Youth Science Foundation of Henan Province(Grant No.202300410136)the Experimental Development Foundation of Henan University of Science and Technology(Grant No.SY2324004)。
文摘Paeonia suffruticosa Andr.is an endemic shrub flower in China with 2n=10.This study used 228 cultivars from four populations,i.e.,Jiangnan,Japan,Northwest,and Zhongyuan,as materials to explore the genetic diversity levels among different populations of tree peony varieties.The results showed that 34 bands were amplified using five pairs of cp SSR primers,with an average of 6.8 bands per primer pair.The average number of different alleles(N_(a)),effective alleles(N_(e)),Shannon's information index(I),diversity(H),and polymorphic information content(PIC)were 3.600,2.053,0.708,0.433,and 0.388,respectively.The PIC value was between 0.250 and 0.500,indicating a moderate level of polymorphism for the five cp SSR primer pairs.The genetic diversity levels of peony cultivars varied among different populations,with the Northwest population showing relatively lower levels(I=0.590,H=0.289,and PIC=0.263).A total of 52 haplotypes were identified in the four examined populations,and the number of haplotypes per population ranged from 11 to 22.Forty-four private haplotypes were detected across populations,and the Northwest population exhibiting the highest count of private haplotypes with 17.The mean number of effective number of haplotypes(N_(eh)),haplotypic richness(R_(h)),and diversity(H)were 8.351,6.824,and 0.893,respectively.Analysis of molecular variance indicated that genetic variation within tree peony germplasm was greater than that between germplasm resources,and the main variation was found within individuals of peony germplasm.Cluster analysis,principal coordinate analysis,and genetic structure analysis classified tree peonies from different origins into two groups,indicating a certain degree of genetic differentiation among these four tree peony cultivation groups.This study provides a theoretical basis for the exploration,utilization,and conservation of peony germplasm resources,as well as for research on the breeding of excellent varieties.
基金Supported by National Scientific and Technical Supporting Project ofStudies on Superior Species Selecting and Breeding Technique ofJatropha curcasLinn(2007BAD50B01)~~
文摘[ Objective] The aim of this study was to establish the optimum cpSSR-PCR system for Jatropha curcas Linn. [ Method] cpSSR-PCR amplification system for Jatropha curcas Linn influenced by five factors including Taq DNA polymerase, Mg^2＋ , DNA template, dNTP and primer were optimized from several levels. [ Result] The optimum concentration of 20 μl reaction system was 10 × Buffer, 2.00 mmol/L Mg^2＋ , 2 U/μl Taq DNA polymerase, 0.2 mmol/L dNTP, 0.2 μmol/L primer and 35 ng/μl DNA template. [ Conclusion] The optimum annealing temperature for cpSSR-PCR reaction system is 52 ℃, and the cpSSR reaction system is steady and reproducible.
文摘三钢6.25 m 3号焦炉采用炭化室压力稳定系统,简称CPS系统,该应用可以实现焦炉无烟装煤以及结焦过程中各炭化室的压力稳定。CPS系统依据装煤、出焦信号,自动完成相应操作,提高了焦炉生产的自动化水平。在投产前期,CPS系统经常出现问题,但通过不断摸索、研究,并针对性地进行改进处理后,相关故障基本上得以消除。
文摘The advent of Industry 5.0 marks a transformative era where Cyber-Physical Systems(CPSs)seamlessly integrate physical processes with advanced digital technologies.However,as industries become increasingly interconnected and reliant on smart digital technologies,the intersection of physical and cyber domains introduces novel security considerations,endangering the entire industrial ecosystem.The transition towards a more cooperative setting,including humans and machines in Industry 5.0,together with the growing intricacy and interconnection of CPSs,presents distinct and diverse security and privacy challenges.In this regard,this study provides a comprehensive review of security and privacy concerns pertaining to CPSs in the context of Industry 5.0.The review commences by providing an outline of the role of CPSs in Industry 5.0 and then proceeds to conduct a thorough review of the different security risks associated with CPSs in the context of Industry 5.0.Afterward,the study also presents the privacy implications inherent in these systems,particularly in light of the massive data collection and processing required.In addition,the paper delineates potential avenues for future research and provides countermeasures to surmount these challenges.Overall,the study underscores the imperative of adopting comprehensive security and privacy strategies within the context of Industry 5.0.
基金the financial support received from NATO under the Emerging Security Challenges Division programthe support received from NPRP (10-0105-17017) from the Qatar National Research Fund (a member of Qatar Foundation)+1 种基金the support received from the Natural Sciences and Engineering Research Council of Canada (NSERC) and the Department of National Defence (DND) under the Discovery Grant and DND Supplemental Programssupported in part by funding from the Innovation for Defence Excellence and Security (IDEaS) program from the Department of National Defence (DND)。
文摘In this paper, we study stealthy cyber-attacks on actuators of cyber-physical systems(CPS), namely zero dynamics and controllable attacks. In particular, under certain assumptions, we investigate and propose conditions under which one can execute zero dynamics and controllable attacks in the CPS. The above conditions are derived based on the Markov parameters of the CPS and elements of the system observability matrix. Consequently, in addition to outlining the number of required actuators to be attacked, these conditions provide one with the minimum system knowledge needed to perform zero dynamics and controllable cyber-attacks. As a countermeasure against the above stealthy cyber-attacks, we develop a dynamic coding scheme that increases the minimum number of the CPS required actuators to carry out zero dynamics and controllable cyber-attacks to its maximum possible value. It is shown that if at least one secure input channel exists, the proposed dynamic coding scheme can prevent adversaries from executing the zero dynamics and controllable attacks even if they have complete knowledge of the coding system. Finally, two illustrative numerical case studies are provided to demonstrate the effectiveness and capabilities of our derived conditions and proposed methodologies.
