Three-dimensional(3D)culture systems are becoming increasingly popular due to their ability to mimic tissue-like structures more effectively than the monolayer cultures.In cancer and stem cell research,the natural cel...Three-dimensional(3D)culture systems are becoming increasingly popular due to their ability to mimic tissue-like structures more effectively than the monolayer cultures.In cancer and stem cell research,the natural cell characteristics and architectures are closely mimicked by the 3D cell models.Thus,the 3D cell cultures are promising and suitable systems for various proposes,ranging from disease modeling to drug target identification as well as potential therapeutic substances that may transform our lives.This review provides a comprehensive compendium of recent advancements in culturing cells,in particular cancer and stem cells,using 3D culture techniques.The major approaches highlighted here include cell spheroids,hydrogel embedding,bioreactors,scaffolds,and bioprinting.In addition,the progress of employing 3D cell culture systems as a platform for cancer and stem cell research was addressed,and the prominent studies of 3D cell culture systems were discussed.展开更多
目的评价基于智慧健康服务平台的糖尿病前期老年人群运动方案对老年人体重指数(BMI)、空腹血糖(FBG)、餐后2 h血糖(2 h PBG)及运动自我效能的影响。方法选取2022年12月至2023年4月于成都某社区医院筛查诊断出的糖尿病前期老年人57例为...目的评价基于智慧健康服务平台的糖尿病前期老年人群运动方案对老年人体重指数(BMI)、空腹血糖(FBG)、餐后2 h血糖(2 h PBG)及运动自我效能的影响。方法选取2022年12月至2023年4月于成都某社区医院筛查诊断出的糖尿病前期老年人57例为研究对象。按照随机数字表法将研究对象分为试验组(n=30)和对照组(n=27),对照组实施基于智慧健康服务平台的常规运动健康宣教,试验组实施基于智慧健康服务平台的运动方案。于干预前、干预第6周末、干预第12周末收集两组BMI、FBG、2 h PBG、运动自我效能评价量表(SEE)得分进行组内、组间比较。结果干预第6周末、第12周末试验组BMI、FBG、2 h PBG水平均低于对照组(P<0.05);试验组SEE得分高于对照组(P<0.05)。结论基于智慧健康服务平台支持的糖尿病前期老年人群的运动方案,能降低老年人FBG、2 h PBG及BMI,提高老年人运动自我效能,为社区慢性病人群数字化管理提供了新思路,同时为糖尿病前期老年人的运动管理提供了参考依据。展开更多
Cancer stem cells(CSCs)are a relatively rare subpopulation of tumor cell with self-renewal and tumorigenesis capabilities.CSCs are associated with cancer recurrence,progression,and chemoradiotherapy resistance.Establi...Cancer stem cells(CSCs)are a relatively rare subpopulation of tumor cell with self-renewal and tumorigenesis capabilities.CSCs are associated with cancer recurrence,progression,and chemoradiotherapy resistance.Establishing a reliable platform for CSC enrichment and study is a prerequisite for understanding the characteristics of CSCs and discovering CSC-related therapeutic strategies.Certain strategies for CSC enrichment have been used in laboratory,particularly fluorescence-activated cell sorting(FACS)and mammosphere culture.However,these methods fail to recapitulate the in vivo chemical and physical conditions in tumors,thus potentially decreasing the malignancy of CSCs in culture and yielding unreliable research results.Accumulating research suggests the promise of a biomaterial-based three-dimensional(3 D)strategy for CSC enrichment and study.This strategy has an advantage over conventional methods in simulating the tumor microenvironment,thus providing a more effective and predictive model for CSC laboratory research.In this review,we first briefly discuss the conventional methods for CSC enrichment and study.We then summarize the latest advances and challenges in biomaterial-based 3 D CSC platforms.Design strategies for materials,morphology,and chemical and physical cues are highlighted to provide direction for the future construction of platforms for CSC enrichment and study.展开更多
Advances in the field of medical sciences and medical technology,and present-day challenges,such as an aging population,rising medical expenses,and lifestyle-related diseases,have collectively catalyzed a research eco...Advances in the field of medical sciences and medical technology,and present-day challenges,such as an aging population,rising medical expenses,and lifestyle-related diseases,have collectively catalyzed a research ecosystem termed“smart wellness.”This article describes the establishment of a smart wellness service platform designed to empower individuals to create a sense of balance in their lives.Step-by-step details include service model,design,and architectural considerations.As a proof of concept,implementation details of a Health Improvement and Management Systems(HIMS)Hub,a Smart Wellness Service Platform deployed in six cities in South Korea,are presented.An on-site survey conducted in Busan Metropolitan City reveals the percentage of satisfied users to be 91.3%.Furthermore,data gathered from 27,236 physical evaluations of users from a Busan city center over the period of April 2013 to May 2018 reveal that males and females in their 50s and 60s account for the highest number of participants,while males in their 70s have a higher rate of participation than females in the same age group.展开更多
文摘Three-dimensional(3D)culture systems are becoming increasingly popular due to their ability to mimic tissue-like structures more effectively than the monolayer cultures.In cancer and stem cell research,the natural cell characteristics and architectures are closely mimicked by the 3D cell models.Thus,the 3D cell cultures are promising and suitable systems for various proposes,ranging from disease modeling to drug target identification as well as potential therapeutic substances that may transform our lives.This review provides a comprehensive compendium of recent advancements in culturing cells,in particular cancer and stem cells,using 3D culture techniques.The major approaches highlighted here include cell spheroids,hydrogel embedding,bioreactors,scaffolds,and bioprinting.In addition,the progress of employing 3D cell culture systems as a platform for cancer and stem cell research was addressed,and the prominent studies of 3D cell culture systems were discussed.
文摘目的评价基于智慧健康服务平台的糖尿病前期老年人群运动方案对老年人体重指数(BMI)、空腹血糖(FBG)、餐后2 h血糖(2 h PBG)及运动自我效能的影响。方法选取2022年12月至2023年4月于成都某社区医院筛查诊断出的糖尿病前期老年人57例为研究对象。按照随机数字表法将研究对象分为试验组(n=30)和对照组(n=27),对照组实施基于智慧健康服务平台的常规运动健康宣教,试验组实施基于智慧健康服务平台的运动方案。于干预前、干预第6周末、干预第12周末收集两组BMI、FBG、2 h PBG、运动自我效能评价量表(SEE)得分进行组内、组间比较。结果干预第6周末、第12周末试验组BMI、FBG、2 h PBG水平均低于对照组(P<0.05);试验组SEE得分高于对照组(P<0.05)。结论基于智慧健康服务平台支持的糖尿病前期老年人群的运动方案,能降低老年人FBG、2 h PBG及BMI,提高老年人运动自我效能,为社区慢性病人群数字化管理提供了新思路,同时为糖尿病前期老年人的运动管理提供了参考依据。
基金supported by grants from the National Natural Science Foundation of China(Grant Nos.11832008 and 81703012)the Program of the Postgraduate Tutor Team of Chongqing Education Commission(2018)+1 种基金the Fundamental Research Funds for the Central Universities(Grant No.2019CDXYSG0004)the Visiting Scholar Foundation of the Key Laboratory of Biorheological Science and Technology(Chongqing University),Ministry of Education(Grant No.CQKLBST-2017-005)。
文摘Cancer stem cells(CSCs)are a relatively rare subpopulation of tumor cell with self-renewal and tumorigenesis capabilities.CSCs are associated with cancer recurrence,progression,and chemoradiotherapy resistance.Establishing a reliable platform for CSC enrichment and study is a prerequisite for understanding the characteristics of CSCs and discovering CSC-related therapeutic strategies.Certain strategies for CSC enrichment have been used in laboratory,particularly fluorescence-activated cell sorting(FACS)and mammosphere culture.However,these methods fail to recapitulate the in vivo chemical and physical conditions in tumors,thus potentially decreasing the malignancy of CSCs in culture and yielding unreliable research results.Accumulating research suggests the promise of a biomaterial-based three-dimensional(3 D)strategy for CSC enrichment and study.This strategy has an advantage over conventional methods in simulating the tumor microenvironment,thus providing a more effective and predictive model for CSC laboratory research.In this review,we first briefly discuss the conventional methods for CSC enrichment and study.We then summarize the latest advances and challenges in biomaterial-based 3 D CSC platforms.Design strategies for materials,morphology,and chemical and physical cues are highlighted to provide direction for the future construction of platforms for CSC enrichment and study.
基金Korea Electric Power Corporation(Grant Number:R18XA02).
文摘Advances in the field of medical sciences and medical technology,and present-day challenges,such as an aging population,rising medical expenses,and lifestyle-related diseases,have collectively catalyzed a research ecosystem termed“smart wellness.”This article describes the establishment of a smart wellness service platform designed to empower individuals to create a sense of balance in their lives.Step-by-step details include service model,design,and architectural considerations.As a proof of concept,implementation details of a Health Improvement and Management Systems(HIMS)Hub,a Smart Wellness Service Platform deployed in six cities in South Korea,are presented.An on-site survey conducted in Busan Metropolitan City reveals the percentage of satisfied users to be 91.3%.Furthermore,data gathered from 27,236 physical evaluations of users from a Busan city center over the period of April 2013 to May 2018 reveal that males and females in their 50s and 60s account for the highest number of participants,while males in their 70s have a higher rate of participation than females in the same age group.
