Inflammation has been shown to play an important role in the progression of Alzheimer＇s disease （AD）. Recent epidemical study indicates that the incidence of AD in some populations is substantially influenced by th...Inflammation has been shown to play an important role in the progression of Alzheimer＇s disease （AD）. Recent epidemical study indicates that the incidence of AD in some populations is substantially influenced by the gene polymorphisms of the inflammation mediators. Meanwhile, an ensured risk factor, the ApoE ε4 allele is also reported to directly promote inflammation. Accordingly, it appears that an individual genetic background has partly determined his predisposition for AD by the extent of the inflammation response to the chronic stimulus by β-amyloid peptide （Aβ） deposits and other antigen stressor in the elderly. Hence we present a hypothesis that the inflammation genotypes may contribute to AD susceptibility. This may provide a new orientation both for future identification of individuals at risk and for personalized medication.展开更多
目的研究通心络对脑缺血再灌注损伤小鼠脑组织神经保护作用的机制。方法将1 26只成年雄性小鼠按随机数字表法分为全程给药组、预处理组及对照组,每组各42只,均建立脑缺血再灌注损伤模型。全程给药组于造模前7d每天连续给予12.5ml/kg剂...目的研究通心络对脑缺血再灌注损伤小鼠脑组织神经保护作用的机制。方法将1 26只成年雄性小鼠按随机数字表法分为全程给药组、预处理组及对照组,每组各42只,均建立脑缺血再灌注损伤模型。全程给药组于造模前7d每天连续给予12.5ml/kg剂量的通心络溶液(0.08g/ml),造模后继续给予相同剂量的通心络至各处死时间点或实验终点;预处理组造模前给药同全程给药组。对照组于造模前7d连续给予等体积的0.9%氯化钠溶液。对造模后1、3、7和14d小鼠脑缺血损伤后神经行为学改变进行改良神经功能评分,并测定造模后1d和3d脑梗死体积、脑组织含水量及细胞因子IL-6、IL-1β、TNF-αmRNA水平。结果全程给药组、预处理组造模后1、3、7、14d的神经功能评分均较对照组低,但仅全程给药组与对照组比较差异有统计学意义(P<0.05);全程给药组、预处理组造模后1、3d的脑梗死体积及脑组织含水量均较对照组小,但仅全程给药组与对照组比较差异有统计学意义(P<0.05);全程给药组造模后1、3d脑组织中IL-6和IL-1 B mRNA水平较对照组低,差异有统计学意义(均P<0.05),TNF-αmRNA水平虽较对照组低,但差异无统计学意义(P>0.05),预处理组中各时点IL-1β、IL-6和TNF-αmRNA水平均较对照组高,但差异无统计学意义(均P>0.05)。结论通心络对脑缺血再灌注损伤有神经保护作用,其作用机制可能是减轻了脑组织中的炎症反应。展开更多
基金the National Basic Research Development Program of China (No. 2006cb500706)the National Natural Science Foundation of China (No. 30700251)+1 种基金Shanghai Key Project of Basic Science Research (No. 04DZ14005)the Program for Outstanding Medical Academic Leader (No. LJ 06003).
文摘Inflammation has been shown to play an important role in the progression of Alzheimer＇s disease （AD）. Recent epidemical study indicates that the incidence of AD in some populations is substantially influenced by the gene polymorphisms of the inflammation mediators. Meanwhile, an ensured risk factor, the ApoE ε4 allele is also reported to directly promote inflammation. Accordingly, it appears that an individual genetic background has partly determined his predisposition for AD by the extent of the inflammation response to the chronic stimulus by β-amyloid peptide （Aβ） deposits and other antigen stressor in the elderly. Hence we present a hypothesis that the inflammation genotypes may contribute to AD susceptibility. This may provide a new orientation both for future identification of individuals at risk and for personalized medication.
文摘目的研究通心络对脑缺血再灌注损伤小鼠脑组织神经保护作用的机制。方法将1 26只成年雄性小鼠按随机数字表法分为全程给药组、预处理组及对照组,每组各42只,均建立脑缺血再灌注损伤模型。全程给药组于造模前7d每天连续给予12.5ml/kg剂量的通心络溶液(0.08g/ml),造模后继续给予相同剂量的通心络至各处死时间点或实验终点;预处理组造模前给药同全程给药组。对照组于造模前7d连续给予等体积的0.9%氯化钠溶液。对造模后1、3、7和14d小鼠脑缺血损伤后神经行为学改变进行改良神经功能评分,并测定造模后1d和3d脑梗死体积、脑组织含水量及细胞因子IL-6、IL-1β、TNF-αmRNA水平。结果全程给药组、预处理组造模后1、3、7、14d的神经功能评分均较对照组低,但仅全程给药组与对照组比较差异有统计学意义(P<0.05);全程给药组、预处理组造模后1、3d的脑梗死体积及脑组织含水量均较对照组小,但仅全程给药组与对照组比较差异有统计学意义(P<0.05);全程给药组造模后1、3d脑组织中IL-6和IL-1 B mRNA水平较对照组低,差异有统计学意义(均P<0.05),TNF-αmRNA水平虽较对照组低,但差异无统计学意义(P>0.05),预处理组中各时点IL-1β、IL-6和TNF-αmRNA水平均较对照组高,但差异无统计学意义(均P>0.05)。结论通心络对脑缺血再灌注损伤有神经保护作用,其作用机制可能是减轻了脑组织中的炎症反应。
