于2023年春季,对厦门杏林湾水库开展了浮游生物群落生态特征的调查,并从水质变化参数与生物多样性健康评价。结果表明,浮游植物4门45种,其中绿藻门种类数最多,占总种类数的58.00%,主要优势种有长刺根管藻、梅尼小环藻、四尾栅藻等;浮游...于2023年春季,对厦门杏林湾水库开展了浮游生物群落生态特征的调查,并从水质变化参数与生物多样性健康评价。结果表明,浮游植物4门45种,其中绿藻门种类数最多,占总种类数的58.00%,主要优势种有长刺根管藻、梅尼小环藻、四尾栅藻等;浮游植物的平均密度和平均生物量分别为9.22 × 104 cells/L和41.5 μg/L。浮游动物共鉴定出4类28种,其中轮虫数量最多,约占50.00%,主要优势种有无节幼体、圆型臂尾轮虫、萼花臂尾轮虫等;浮游动物的平均密度和平均生物量分别为55.51 ind/L和0.542 mg/L。综合浮游植物和浮游动物的调查结果,根据生物多样性水质评价标准可知杏林湾水库的水质整体较差,浮游生物丰富度较低。The plankton in Xinglinwan Reservoir was investigated and health evaluation was studied from water quality change parameters and biodiversity in the spring of 2022. The results showed that there were a total of 45 species of phytoplankton belonging to 4 phyla, among which Chlorophyta accounted for 58.00% of the total phytoplankton species, the main dominant species were Rhizosolenia longiseta, Cyclotella meneghiniana, Scenedesmus quqdricauda, etc. The average density and average biomass of phytoplankton were 9.22104 cells/L and 41.5 μg/L, respectively. There were 28 species of zooplankton belonging to 4 groups, the number of rotifera was the most, accounting for50.00% of the total zooplankton species, the main dominant species were Nauplius, Brachionus rotundiformis, Brachionus calyciflorus, etc. The average density and average biomass of zooplankton were 55.51 ind/L and 0.542 mg/L, respectively. Based on the survey results of phytoplankton and zooplankton, the water quality evaluation standard of biodiversity shows that the water quality of Xinglinwan Reservoir is poor and the plankton richness is low.展开更多
为探究一种包含泛素调节性X结构域的蛋白(ubiquitin regulatory X domain-containing protein,UBXN1)在大黄鱼抗盾纤毛虫感染中的作用,以及可能涉及的免疫信号通路。本实验克隆鉴定了大黄鱼UBXN1基因,并利用在线软件对其序列特征进行生...为探究一种包含泛素调节性X结构域的蛋白(ubiquitin regulatory X domain-containing protein,UBXN1)在大黄鱼抗盾纤毛虫感染中的作用,以及可能涉及的免疫信号通路。本实验克隆鉴定了大黄鱼UBXN1基因,并利用在线软件对其序列特征进行生物信息学分析;采用实时荧光定量PCR(qRT-PCR)检测UBXN1在健康大黄鱼各组织中的表达,及盾纤毛虫感染后的诱导表达变化;并进行了UBXN1的亚细胞定位;转录组测序分析了UBXN1过表达前后的差异表达基因。结果显示,UBXN1基因cDNA全长为915 bp,编码304个氨基酸。蛋白多重序列比对和结构预测表明UBXN1是一个进化保守的蛋白,包含UBA和UBX结构域。qRT-PCR分析表明UBXN1在所检测的11种组织中均有表达,脑中表达量最高,其次是肝脏、心脏和肾脏,在肌肉中表达量最低;盾纤毛虫感染大黄鱼后,UBXN1在脾脏、脑、肝脏和肾脏中表达量早期显著升高,后期逐步恢复至正常水平。亚细胞定位分析表明,UBXN1在大黄鱼肾脏细胞质和细胞核中均有表达。在293T细胞过表达UBXN1,转录组差异表达分析筛选到12个上调基因,4个下调基因,其中RPL41/RPL39/XIST/RNA45SN4表达量显著增加,而ATP8/ND4L表达量显著减少。研究表明UBXN1在大黄鱼抗寄生虫免疫应答中发挥重要作用。本实验为进一步研究UBXN1的免疫信号通路奠定基础。展开更多
文摘于2023年春季,对厦门杏林湾水库开展了浮游生物群落生态特征的调查,并从水质变化参数与生物多样性健康评价。结果表明,浮游植物4门45种,其中绿藻门种类数最多,占总种类数的58.00%,主要优势种有长刺根管藻、梅尼小环藻、四尾栅藻等;浮游植物的平均密度和平均生物量分别为9.22 × 104 cells/L和41.5 μg/L。浮游动物共鉴定出4类28种,其中轮虫数量最多,约占50.00%,主要优势种有无节幼体、圆型臂尾轮虫、萼花臂尾轮虫等;浮游动物的平均密度和平均生物量分别为55.51 ind/L和0.542 mg/L。综合浮游植物和浮游动物的调查结果,根据生物多样性水质评价标准可知杏林湾水库的水质整体较差,浮游生物丰富度较低。The plankton in Xinglinwan Reservoir was investigated and health evaluation was studied from water quality change parameters and biodiversity in the spring of 2022. The results showed that there were a total of 45 species of phytoplankton belonging to 4 phyla, among which Chlorophyta accounted for 58.00% of the total phytoplankton species, the main dominant species were Rhizosolenia longiseta, Cyclotella meneghiniana, Scenedesmus quqdricauda, etc. The average density and average biomass of phytoplankton were 9.22104 cells/L and 41.5 μg/L, respectively. There were 28 species of zooplankton belonging to 4 groups, the number of rotifera was the most, accounting for50.00% of the total zooplankton species, the main dominant species were Nauplius, Brachionus rotundiformis, Brachionus calyciflorus, etc. The average density and average biomass of zooplankton were 55.51 ind/L and 0.542 mg/L, respectively. Based on the survey results of phytoplankton and zooplankton, the water quality evaluation standard of biodiversity shows that the water quality of Xinglinwan Reservoir is poor and the plankton richness is low.
