N6-甲基腺嘌呤(N6-methyladenosine,m6A)是真核生物信使RNA(Messenger RNA,m RNA)上含量最多的化学修饰之一。类似于DNA和组蛋白化学修饰,m6A修饰也同样是动态可逆的,可在时间和空间上被甲基转移酶和去甲基酶调控。哺乳动物体内m6A甲基...N6-甲基腺嘌呤(N6-methyladenosine,m6A)是真核生物信使RNA(Messenger RNA,m RNA)上含量最多的化学修饰之一。类似于DNA和组蛋白化学修饰,m6A修饰也同样是动态可逆的,可在时间和空间上被甲基转移酶和去甲基酶调控。哺乳动物体内m6A甲基转移酶复合物中有一部分成分已被解析,主要有METTL3(Methyltransferase-like protein 3)、METTL14(Methyltransferase-like protein 14)和WTAP(Wilms tumor 1-associating protein)。m6A去甲基酶肥胖蛋白FTO(Fat mass and obesity associated protein)和ALKBH5(Alk B homolog 5)依赖α-酮戊二酸(α-Ketoglutaric acid,α-KG)和Fe(Ⅱ)对m6A进行氧化去甲基化反应。m6A在生物体内由m6A结合蛋白识别,并介导其行使功能。目前发现的m6A结合蛋白有YTH结构域蛋白YTHDF1(YTH domain-containing family protein 1)、YTHDF2(YTH domain-containing family protein 2)、YTHDC1(YTH domain-containing protein1)和核内HNRNPA2B1(Heterogeneous nuclear ribonucleoproteins A2B1)。本文综述了m6A的分布和相关蛋白介导的m6A功能研究,以期全面理解m6A这一RNA表观遗传新修饰在生命进程中的重要调控作用。展开更多
We found an ultraviolet （UV）-light induced formation of biphenyl and sodium benzoate from benzene and sodium carbonate. The reaction happens in the interface of benzene and aqueous solution at the room temperature. ...We found an ultraviolet （UV）-light induced formation of biphenyl and sodium benzoate from benzene and sodium carbonate. The reaction happens in the interface of benzene and aqueous solution at the room temperature. After 5 h of UV-light exposure, 11.4% of initial amount of 4.4 g （5.0 mL） benzene are converted to biphenyl and sodium benzoate, which are distributed in benzene and aqueous solution, respectively. Using density function theory （DFT） and time dependent DFT, we have investigated the mechanism of this light-induced reaction, and found that the sodium carbonate is not only a reactant for the formation of sodium benzoate, but also a catalyst for the formation of biphenyl.展开更多
The development of a simple and efficient method, which allows to fast screen compounds with high biological activity from natural plant extracts, is an attractive field in drug discovery. Our group propounded a novel...The development of a simple and efficient method, which allows to fast screen compounds with high biological activity from natural plant extracts, is an attractive field in drug discovery. Our group propounded a novel method for fast screening and structural identification of antitumor agents from natural plant extracts by coupling the DNA target molecule’s recognition with NMR techniques. By introduction of DNA target molecule into natural plant extracts, the ligand(s) in the extracts will be recognized by target molecule and therefore can be monitored by NMR techniques. Our tactics includes the following three steps: judging the existence of G-quadruplex ligand(s) in the test extracts by 1H NMR spectra; detecting the characteristic peak(s) of G-quadruplex ligand(s) using DOSY NMR methods; and subsequently identifying the structure of G-quadruplex ligand(s) by 2D NMR experiments such as HSQC and HMBC. By the combination of G-quadruplex recognition and NMR methods, we have successfully obtained several active molecules stabilizing G-quadruplex with high cytotoxicity from two kinds of natural product extracts.展开更多
Unique three-dimensionally (3D) ordered nanoporous calcite single crystals have been fabricated by exploiting the "amorphous to crystalline" strategy in combination with the colloidal crystal templating meth...Unique three-dimensionally (3D) ordered nanoporous calcite single crystals have been fabricated by exploiting the "amorphous to crystalline" strategy in combination with the colloidal crystal templating method. It was revealed that the surface-functionalized template and the vacuum-assisted filtration process were essential for the complete template replication. Such a bio-inspired strategy may suggest a general strategy for the fabrication of novel functional single-crystalline materials with 3D ordered nanopatterns.展开更多
文摘N6-甲基腺嘌呤(N6-methyladenosine,m6A)是真核生物信使RNA(Messenger RNA,m RNA)上含量最多的化学修饰之一。类似于DNA和组蛋白化学修饰,m6A修饰也同样是动态可逆的,可在时间和空间上被甲基转移酶和去甲基酶调控。哺乳动物体内m6A甲基转移酶复合物中有一部分成分已被解析,主要有METTL3(Methyltransferase-like protein 3)、METTL14(Methyltransferase-like protein 14)和WTAP(Wilms tumor 1-associating protein)。m6A去甲基酶肥胖蛋白FTO(Fat mass and obesity associated protein)和ALKBH5(Alk B homolog 5)依赖α-酮戊二酸(α-Ketoglutaric acid,α-KG)和Fe(Ⅱ)对m6A进行氧化去甲基化反应。m6A在生物体内由m6A结合蛋白识别,并介导其行使功能。目前发现的m6A结合蛋白有YTH结构域蛋白YTHDF1(YTH domain-containing family protein 1)、YTHDF2(YTH domain-containing family protein 2)、YTHDC1(YTH domain-containing protein1)和核内HNRNPA2B1(Heterogeneous nuclear ribonucleoproteins A2B1)。本文综述了m6A的分布和相关蛋白介导的m6A功能研究,以期全面理解m6A这一RNA表观遗传新修饰在生命进程中的重要调控作用。
文摘We found an ultraviolet （UV）-light induced formation of biphenyl and sodium benzoate from benzene and sodium carbonate. The reaction happens in the interface of benzene and aqueous solution at the room temperature. After 5 h of UV-light exposure, 11.4% of initial amount of 4.4 g （5.0 mL） benzene are converted to biphenyl and sodium benzoate, which are distributed in benzene and aqueous solution, respectively. Using density function theory （DFT） and time dependent DFT, we have investigated the mechanism of this light-induced reaction, and found that the sodium carbonate is not only a reactant for the formation of sodium benzoate, but also a catalyst for the formation of biphenyl.
文摘The development of a simple and efficient method, which allows to fast screen compounds with high biological activity from natural plant extracts, is an attractive field in drug discovery. Our group propounded a novel method for fast screening and structural identification of antitumor agents from natural plant extracts by coupling the DNA target molecule’s recognition with NMR techniques. By introduction of DNA target molecule into natural plant extracts, the ligand(s) in the extracts will be recognized by target molecule and therefore can be monitored by NMR techniques. Our tactics includes the following three steps: judging the existence of G-quadruplex ligand(s) in the test extracts by 1H NMR spectra; detecting the characteristic peak(s) of G-quadruplex ligand(s) using DOSY NMR methods; and subsequently identifying the structure of G-quadruplex ligand(s) by 2D NMR experiments such as HSQC and HMBC. By the combination of G-quadruplex recognition and NMR methods, we have successfully obtained several active molecules stabilizing G-quadruplex with high cytotoxicity from two kinds of natural product extracts.
文摘Unique three-dimensionally (3D) ordered nanoporous calcite single crystals have been fabricated by exploiting the "amorphous to crystalline" strategy in combination with the colloidal crystal templating method. It was revealed that the surface-functionalized template and the vacuum-assisted filtration process were essential for the complete template replication. Such a bio-inspired strategy may suggest a general strategy for the fabrication of novel functional single-crystalline materials with 3D ordered nanopatterns.
