The plasma membrane fortion isolated from primp leaves of 14 d soybean shoot showed Protein kinase (PK) and protein phosphatase (PP) activities (Fig. 1 ), and these activities declined dramaically when the leaves unde...The plasma membrane fortion isolated from primp leaves of 14 d soybean shoot showed Protein kinase (PK) and protein phosphatase (PP) activities (Fig. 1 ), and these activities declined dramaically when the leaves underwent senescence-inducing treatment (Table 1 ). 6-BA pretreatment, which showed retarding effect on soybean leaf senescence, could sustain the activities of both PKs and PPs during the senescence-inducing treatment (Table 1 ). The results of autoradiogra phy suggested that the main PKs whose activities declined during leaf senescence were calcium-dependent Protein kinases (Fig. 3). The potent and specif ic inhibitor of Protein phosphatase 1 and 2A, okadric acid, could fasten leaf senescence. These results impied thatretaining the balance of plasma membrane protein phosphorylation/ dephosphorylation was the key action of exogenous 6-BA in retarding leat senescence.展开更多
In order to understand the physiological and biochemical reasons of leafcolor transformation in Loropetalum chinense var. rubrum,three types of L. chinense var. rubrum, Zihei (black purple), Zihong(red purple)and Hong...In order to understand the physiological and biochemical reasons of leafcolor transformation in Loropetalum chinense var. rubrum,three types of L. chinense var. rubrum, Zihei (black purple), Zihong(red purple)and Hongzhe(red_ochre), and L. chinense were taken as materials. The contents of chlorophyll, carotenoid and soluble sugar, and activity of phenylalanine ammonialyase (PLA) in the leaves were measured from early March to June. The results showed that the contents of chlorophyll, carotenoid and soluble sugar increased gradually during the leafcolor changing from red to green, but there were obviously differences among types of Zihei, Zihong and Hongzhe. Anthocyanins contents in Zihe and Zihong leaves maintained high level, so their leaves still kept red when they got mature in early June. Phenylalanine ammonialyase (PLA) activity decreased, and had positive relationship with anthocyanin contents. Some measures such as shading or watering to lower air temperature could be taken to maintain leaves of L. chinense var. rubrum fresh red during summer.展开更多
目的 研究不同 p H值对东北红豆杉和南方红豆杉愈伤组织生长和紫杉醇含量的影响。方法 采用不同p H值的 B5培养基培养两种红豆杉愈伤组织 ,测定生长率、PAL 活性和紫杉醇含量。结果 不同红豆杉愈伤组织所需最适 p H值不同 ,而有利于...目的 研究不同 p H值对东北红豆杉和南方红豆杉愈伤组织生长和紫杉醇含量的影响。方法 采用不同p H值的 B5培养基培养两种红豆杉愈伤组织 ,测定生长率、PAL 活性和紫杉醇含量。结果 不同红豆杉愈伤组织所需最适 p H值不同 ,而有利于愈伤组织生长的 p H值均不利于 PAL 活性的提高和紫杉醇的积累。即 :愈伤组织生长快时 ,PAL活性将下降 ,紫杉醇含量也减少。结论 p H值明显影响红豆杉愈伤组织的生长及次级代谢水平 。展开更多
CaM BP-10 (BP-10) is anovel endogenous calmodalin (CaM )-binding Protein tha was observed in andisolated from Plant. In our prelidrimpstudies it has been shown tha BP-10specifically inhibits adrin (IAA)-in-duced coleo...CaM BP-10 (BP-10) is anovel endogenous calmodalin (CaM )-binding Protein tha was observed in andisolated from Plant. In our prelidrimpstudies it has been shown tha BP-10specifically inhibits adrin (IAA)-in-duced coleoptile elongallon and pbonsecrehon. In oIder to inveshgatewhether BP10 inhibitS Picton secrehonand cell elongaion thidgh inhibitingthc achvity of Plasrna mernbare (PM)H+ -ATPase, the effect of BP-10 on theachvity of wheat coleoptile PM H+ -AT-Pase has been studied. It was observedtha neither IAA nor BP-10 had any effect on the achvity of PM H+ -ATPaSein vitro. In vho, however, the activity0f Al-treated PM H+-ATPaSe washigher than tha of the control, and BP-10 peilicanx iwhbital atinduedachvity by 87- 8% (rig. 1) - In addi-hon, the inhibitory effect cotild be re-versed by IAA after BPIO was removedfrom the mediurn, and it could also beovercome bV the addihon of CaM.Therefore, it appears that BP-10 in-hibits cell responses to andn by affect-ing the achvity of PM H+ -ATPase andthereby inhibiting Proton secrehon. As aresult, cell elongaon is inhibited. AndCaM is also involved in this pmeess-展开更多
文摘The plasma membrane fortion isolated from primp leaves of 14 d soybean shoot showed Protein kinase (PK) and protein phosphatase (PP) activities (Fig. 1 ), and these activities declined dramaically when the leaves underwent senescence-inducing treatment (Table 1 ). 6-BA pretreatment, which showed retarding effect on soybean leaf senescence, could sustain the activities of both PKs and PPs during the senescence-inducing treatment (Table 1 ). The results of autoradiogra phy suggested that the main PKs whose activities declined during leaf senescence were calcium-dependent Protein kinases (Fig. 3). The potent and specif ic inhibitor of Protein phosphatase 1 and 2A, okadric acid, could fasten leaf senescence. These results impied thatretaining the balance of plasma membrane protein phosphorylation/ dephosphorylation was the key action of exogenous 6-BA in retarding leat senescence.
文摘In order to understand the physiological and biochemical reasons of leafcolor transformation in Loropetalum chinense var. rubrum,three types of L. chinense var. rubrum, Zihei (black purple), Zihong(red purple)and Hongzhe(red_ochre), and L. chinense were taken as materials. The contents of chlorophyll, carotenoid and soluble sugar, and activity of phenylalanine ammonialyase (PLA) in the leaves were measured from early March to June. The results showed that the contents of chlorophyll, carotenoid and soluble sugar increased gradually during the leafcolor changing from red to green, but there were obviously differences among types of Zihei, Zihong and Hongzhe. Anthocyanins contents in Zihe and Zihong leaves maintained high level, so their leaves still kept red when they got mature in early June. Phenylalanine ammonialyase (PLA) activity decreased, and had positive relationship with anthocyanin contents. Some measures such as shading or watering to lower air temperature could be taken to maintain leaves of L. chinense var. rubrum fresh red during summer.
文摘目的 研究不同 p H值对东北红豆杉和南方红豆杉愈伤组织生长和紫杉醇含量的影响。方法 采用不同p H值的 B5培养基培养两种红豆杉愈伤组织 ,测定生长率、PAL 活性和紫杉醇含量。结果 不同红豆杉愈伤组织所需最适 p H值不同 ,而有利于愈伤组织生长的 p H值均不利于 PAL 活性的提高和紫杉醇的积累。即 :愈伤组织生长快时 ,PAL活性将下降 ,紫杉醇含量也减少。结论 p H值明显影响红豆杉愈伤组织的生长及次级代谢水平 。
文摘CaM BP-10 (BP-10) is anovel endogenous calmodalin (CaM )-binding Protein tha was observed in andisolated from Plant. In our prelidrimpstudies it has been shown tha BP-10specifically inhibits adrin (IAA)-in-duced coleoptile elongallon and pbonsecrehon. In oIder to inveshgatewhether BP10 inhibitS Picton secrehonand cell elongaion thidgh inhibitingthc achvity of Plasrna mernbare (PM)H+ -ATPase, the effect of BP-10 on theachvity of wheat coleoptile PM H+ -AT-Pase has been studied. It was observedtha neither IAA nor BP-10 had any effect on the achvity of PM H+ -ATPaSein vitro. In vho, however, the activity0f Al-treated PM H+-ATPaSe washigher than tha of the control, and BP-10 peilicanx iwhbital atinduedachvity by 87- 8% (rig. 1) - In addi-hon, the inhibitory effect cotild be re-versed by IAA after BPIO was removedfrom the mediurn, and it could also beovercome bV the addihon of CaM.Therefore, it appears that BP-10 in-hibits cell responses to andn by affect-ing the achvity of PM H+ -ATPase andthereby inhibiting Proton secrehon. As aresult, cell elongaon is inhibited. AndCaM is also involved in this pmeess-
