目的:探讨虎杖苷对大鼠激素性股骨头坏死(steroid-induced osteonecrosis of the femoral head,SONFH)的治疗效果及其通过调节Janus激酶2/信号转导和转录激活因子3信号通路(janus kinase 2/signal transducer and activator of transcri...目的:探讨虎杖苷对大鼠激素性股骨头坏死(steroid-induced osteonecrosis of the femoral head,SONFH)的治疗效果及其通过调节Janus激酶2/信号转导和转录激活因子3信号通路(janus kinase 2/signal transducer and activator of transcription 3 signaling pathway,JAK2/STAT3)影响骨组织保护的潜在机制。方法:5周雄性SD大鼠50只随机分为对照组、模型组、虎杖苷低剂量组(虎杖苷-L)、虎杖苷高剂量组(虎杖苷-H)及虎杖苷-H+Colivelin(JAK2/STAT3通路激活剂)组,每组10只。通过脂多糖和醋酸泼尼松龙诱导SONFH模型。治疗组在模型组基础上每日分别灌胃给予虎杖苷-L 10 mg·kg^(-1),虎杖苷-H 20 mg·kg^(-1),虎杖苷-H+Colivelin组腹腔注射Colivelin 1 mg·kg^(-1),对照组和模型组给予等体积生理盐水,持续6周。观察指标包括血清钙(calcium,Ca)、血清磷(phosphorus,P)、碱性磷酸酶(alkaline phosphatase,ALP)及转化生长因子β1(transforming growth factorβ1,TGF-β1)水平,micro-CT扫描,苏木精-伊红染色及Western blot检测JAK2/STAT3信号通路和成骨分化标志基因Runt相关转录因子2(runt-related transcription factor 2,Runx2)、骨形态发生蛋白2(bone morphogenetic protein 2,BMP2)、骨桥蛋白(osteopontin,OPN)蛋白表达。结果:与模型组相比,虎杖苷-L和虎杖苷-H组大鼠骨小梁面积百分比显著提升,空骨陷窝率显著下降(P<0.05)。Micro-CT分析表明,虎杖苷处理组骨体积分数、骨小梁数量和厚度增加,骨小梁分离度降低(P<0.05)。血清生化指标检测发现,虎杖苷-L和虎杖苷-H组大鼠血清Ca、P浓度恢复,碱性磷酸酶水平降低,转化生长因子β1水平升高(P<0.05)。Western blot分析显示,虎杖苷显著抑制了模型组中JAK2/STAT3信号通路的激活,同时促进了Runx2、BMP2、OPN等成骨分化标志基因的表达(P<0.05)。与虎杖苷-H组相比,虎杖苷-H+Colivelin组的这些改善效果有所减弱,显示JAK2/STAT3信号通路在虎杖苷作用中的重要性。结论:虎杖苷通过调节JAK2/STAT3信号通路,促进成骨分化,改善骨微观结构,对大鼠SONFH具有显著的治疗效果。展开更多
唐宗海《血证论》是中医诊治血证专书,对血证的分类条理清晰,其理论见解新颖。书中对肺系疾病辨证独到,用药效果显著。本文通过梳理分析《血证论》治疗肺系疾病的遣方用药规律,结合阴阳水火气血论,从气血同治、气水同治、气火同治、血...唐宗海《血证论》是中医诊治血证专书,对血证的分类条理清晰,其理论见解新颖。书中对肺系疾病辨证独到,用药效果显著。本文通过梳理分析《血证论》治疗肺系疾病的遣方用药规律,结合阴阳水火气血论,从气血同治、气水同治、气火同治、血火同治、气血水火同治等五个方面探究并总结唐氏治疗肺系疾病的用药规律和经验,为临床治疗提供参考。Tang Zonghai’s Blood Syndrome Theory is a special book on the diagnosis and treatment of blood syndrome in traditional Chinese medicine. The book is unique in the differentiation of pulmonary diseases and the effect of medication is remarkable. This paper explores and summarizes the medication principles and clinical experience of Tang’s approach to treating pulmonary diseases through analyzing the therapeutic strategies in Blood Syndrome Theory. Combining the theory of Yin, Yang, water, fire, Qi and blood interactions, the study investigates five therapeutic dimensions: Qi and blood co-therapy, Qi and water co-therapy, Qi and fire co-therapy, blood and water co-therapy, Qi-blood-water and fire co-therapy, and comprehensive treatment addressing Yin, Yang, water, fire, Qi and blood. These findings aim to provide valuable references for clinical practice in pulmonary disease management.展开更多
文摘唐宗海《血证论》是中医诊治血证专书,对血证的分类条理清晰,其理论见解新颖。书中对肺系疾病辨证独到,用药效果显著。本文通过梳理分析《血证论》治疗肺系疾病的遣方用药规律,结合阴阳水火气血论,从气血同治、气水同治、气火同治、血火同治、气血水火同治等五个方面探究并总结唐氏治疗肺系疾病的用药规律和经验,为临床治疗提供参考。Tang Zonghai’s Blood Syndrome Theory is a special book on the diagnosis and treatment of blood syndrome in traditional Chinese medicine. The book is unique in the differentiation of pulmonary diseases and the effect of medication is remarkable. This paper explores and summarizes the medication principles and clinical experience of Tang’s approach to treating pulmonary diseases through analyzing the therapeutic strategies in Blood Syndrome Theory. Combining the theory of Yin, Yang, water, fire, Qi and blood interactions, the study investigates five therapeutic dimensions: Qi and blood co-therapy, Qi and water co-therapy, Qi and fire co-therapy, blood and water co-therapy, Qi-blood-water and fire co-therapy, and comprehensive treatment addressing Yin, Yang, water, fire, Qi and blood. These findings aim to provide valuable references for clinical practice in pulmonary disease management.
