Objective INF2 is a member of the formins family.Abnormal expression and regulation of INF2 have been associated with the progression of various tumors,but the expression and role of INF2 in hepatocellular carcinoma(H...Objective INF2 is a member of the formins family.Abnormal expression and regulation of INF2 have been associated with the progression of various tumors,but the expression and role of INF2 in hepatocellular carcinoma(HCC)remain unclear.HCC is a highly lethal malignant tumor.Given the limitations of traditional treatments,this study explored the expression level,clinical value and potential mechanism of INF2 in HCC in order to seek new therapeutic targets.Methods In this study,we used public databases to analyze the expression of INF2 in pan-cancer and HCC,as well as the impact of INF2 expression levels on HCC prognosis.Quantitative real time polymerase chain reaction(RT-qPCR),Western blot,and immunohistochemistry were used to detect the expression level of INF2 in liver cancer cells and human HCC tissues.The correlation between INF2 expression and clinical pathological features was analyzed using public databases and clinical data of human HCC samples.Subsequently,the effects of INF2 expression on the biological function and Drp1 phosphorylation of liver cancer cells were elucidated through in vitro and in vivo experiments.Finally,the predictive value and potential mechanism of INF2 in HCC were further analyzed through database and immunohistochemical experiments.Results INF2 is aberrantly high expression in HCC samples and the high expression of INF2 is correlated with overall survival,liver cirrhosis and pathological differentiation of HCC patients.The expression level of INF2 has certain diagnostic value in predicting the prognosis and pathological differentiation of HCC.In vivo and in vitro HCC models,upregulated expression of INF2 triggers the proliferation and migration of the HCC cell,while knockdown of INF2 could counteract this effect.INF2 in liver cancer cells may affect mitochondrial division by inducing Drp1 phosphorylation and mediate immune escape by up-regulating PD-L1 expression,thus promoting tumor progression.Conclusion INF2 is highly expressed in HCC and is associated with poor prognosis.High expression of INF2 may promote HCC progression by inducing Drp1 phosphorylation and up-regulation of PD-L1 expression,and targeting INF2 may be beneficial for HCC patients with high expression of INF2.展开更多
目的探讨分层缝合技术在两孔腹腔镜胆囊切除术+胆总管切开取石术中的应用价值。方法 2014年8月至2017年8月,对宁波市医疗中心李惠利医院267例胆总管结石患者施行两孔腹腔镜胆囊切除术+胆总管切开取石术,并进行前瞻性研究。267例中,110...目的探讨分层缝合技术在两孔腹腔镜胆囊切除术+胆总管切开取石术中的应用价值。方法 2014年8月至2017年8月,对宁波市医疗中心李惠利医院267例胆总管结石患者施行两孔腹腔镜胆囊切除术+胆总管切开取石术,并进行前瞻性研究。267例中,110例采用分层缝合技术,157例采用单层缝合技术。比较两组手术时间、术后住院时间和术后并发症。结果两组均顺利完成手术,无一例中转开腹。两组手术时间没有明显差异(t=-0.587,P=0.086)。分层缝合组术后住院时间和术后胆漏的发生率明显低于单层缝合组[(7.6±1.8)d vs (5.8±1.7)d,t=2.776,P<0.001;4.5%(5/110) vs 20.4%(32/157),χ~2=13.590,P<0.001]。在单层缝合组中,合并急性胆管炎患者的术后胆漏率明显高于无急性胆管炎患者[44.4%(12/27)vs 15.4%(20/130),χ~2=11.634,P=0.001];在分层缝合组中,有无重症胆管炎对患者术后胆漏的发生率不存在明显影响[11.8%(2/17) vs 3.2%(3/93),χ~2=0.848,P=0.357]。结论采用分层缝合技术在两孔腹腔镜胆囊切除术+胆总管切开取石术中是可行且安全的,其优点是减少了胆管的渗漏,缩短了住院时间。展开更多
目的探讨腹腔镜超声(laparoscopic ultrasound,LUS)在腹腔镜左半肝切除术(laparoscopic left hemi-hepatectomy,LLH)中的应用价值。方法选取2012年3月至2014年3月我院肝胆微创外科收治的66例行腹腔镜左半肝切除术患者的临床资料。根据...目的探讨腹腔镜超声(laparoscopic ultrasound,LUS)在腹腔镜左半肝切除术(laparoscopic left hemi-hepatectomy,LLH)中的应用价值。方法选取2012年3月至2014年3月我院肝胆微创外科收治的66例行腹腔镜左半肝切除术患者的临床资料。根据是否使用LUS分为两组:超声组36例,术中使用LUS;对照组30例,未使用LUS者。比较两组患者的手术时间、术中出血量、肝中静脉损伤率、术后并发症、术后住院时间等。结果与对照组比,超声组术中出血量、肝中静脉损伤率、中转开腹率减少,差异有统计学意义(P<0.05);但手术时间、术后并发症、术后住院时间差异无统计学意义(P>0.05)。结论在腹腔镜左半肝切除术中使用LUS可以有效减少术中出血量、肝中静脉损伤从而避免中转开腹,但并不会延长手术时间,不会增加术后并发症发生率,不会缩短住院时间。LUS可以为腹腔镜左半肝切除手术提供"指引",增加了手术的安全性,值得临床推广。展开更多
文摘Objective INF2 is a member of the formins family.Abnormal expression and regulation of INF2 have been associated with the progression of various tumors,but the expression and role of INF2 in hepatocellular carcinoma(HCC)remain unclear.HCC is a highly lethal malignant tumor.Given the limitations of traditional treatments,this study explored the expression level,clinical value and potential mechanism of INF2 in HCC in order to seek new therapeutic targets.Methods In this study,we used public databases to analyze the expression of INF2 in pan-cancer and HCC,as well as the impact of INF2 expression levels on HCC prognosis.Quantitative real time polymerase chain reaction(RT-qPCR),Western blot,and immunohistochemistry were used to detect the expression level of INF2 in liver cancer cells and human HCC tissues.The correlation between INF2 expression and clinical pathological features was analyzed using public databases and clinical data of human HCC samples.Subsequently,the effects of INF2 expression on the biological function and Drp1 phosphorylation of liver cancer cells were elucidated through in vitro and in vivo experiments.Finally,the predictive value and potential mechanism of INF2 in HCC were further analyzed through database and immunohistochemical experiments.Results INF2 is aberrantly high expression in HCC samples and the high expression of INF2 is correlated with overall survival,liver cirrhosis and pathological differentiation of HCC patients.The expression level of INF2 has certain diagnostic value in predicting the prognosis and pathological differentiation of HCC.In vivo and in vitro HCC models,upregulated expression of INF2 triggers the proliferation and migration of the HCC cell,while knockdown of INF2 could counteract this effect.INF2 in liver cancer cells may affect mitochondrial division by inducing Drp1 phosphorylation and mediate immune escape by up-regulating PD-L1 expression,thus promoting tumor progression.Conclusion INF2 is highly expressed in HCC and is associated with poor prognosis.High expression of INF2 may promote HCC progression by inducing Drp1 phosphorylation and up-regulation of PD-L1 expression,and targeting INF2 may be beneficial for HCC patients with high expression of INF2.
文摘目的探讨分层缝合技术在两孔腹腔镜胆囊切除术+胆总管切开取石术中的应用价值。方法 2014年8月至2017年8月,对宁波市医疗中心李惠利医院267例胆总管结石患者施行两孔腹腔镜胆囊切除术+胆总管切开取石术,并进行前瞻性研究。267例中,110例采用分层缝合技术,157例采用单层缝合技术。比较两组手术时间、术后住院时间和术后并发症。结果两组均顺利完成手术,无一例中转开腹。两组手术时间没有明显差异(t=-0.587,P=0.086)。分层缝合组术后住院时间和术后胆漏的发生率明显低于单层缝合组[(7.6±1.8)d vs (5.8±1.7)d,t=2.776,P<0.001;4.5%(5/110) vs 20.4%(32/157),χ~2=13.590,P<0.001]。在单层缝合组中,合并急性胆管炎患者的术后胆漏率明显高于无急性胆管炎患者[44.4%(12/27)vs 15.4%(20/130),χ~2=11.634,P=0.001];在分层缝合组中,有无重症胆管炎对患者术后胆漏的发生率不存在明显影响[11.8%(2/17) vs 3.2%(3/93),χ~2=0.848,P=0.357]。结论采用分层缝合技术在两孔腹腔镜胆囊切除术+胆总管切开取石术中是可行且安全的,其优点是减少了胆管的渗漏,缩短了住院时间。
文摘目的探讨腹腔镜超声(laparoscopic ultrasound,LUS)在腹腔镜左半肝切除术(laparoscopic left hemi-hepatectomy,LLH)中的应用价值。方法选取2012年3月至2014年3月我院肝胆微创外科收治的66例行腹腔镜左半肝切除术患者的临床资料。根据是否使用LUS分为两组:超声组36例,术中使用LUS;对照组30例,未使用LUS者。比较两组患者的手术时间、术中出血量、肝中静脉损伤率、术后并发症、术后住院时间等。结果与对照组比,超声组术中出血量、肝中静脉损伤率、中转开腹率减少,差异有统计学意义(P<0.05);但手术时间、术后并发症、术后住院时间差异无统计学意义(P>0.05)。结论在腹腔镜左半肝切除术中使用LUS可以有效减少术中出血量、肝中静脉损伤从而避免中转开腹,但并不会延长手术时间,不会增加术后并发症发生率,不会缩短住院时间。LUS可以为腹腔镜左半肝切除手术提供"指引",增加了手术的安全性,值得临床推广。
