The complex mechanism of degenerative diseases and the non-specific modulation of regenerative targets aretopics that need to be elucidated in order to advance the use of stem cells in improvement of neurodegenerative...The complex mechanism of degenerative diseases and the non-specific modulation of regenerative targets aretopics that need to be elucidated in order to advance the use of stem cells in improvement of neurodegenerative diseases.From pre-transplantation through post-transplantation, there are many changes in the conditions, both inside andoutside of the stem cells that have not been carefully considered. This has hindered development in the field of celltherapy and regeneration. This viewpoint highlights the potential implications of intracellular and extracellularalterations of stem cells in transplanted areas at risk of neurodegenerative disease.展开更多
For the convenience of people with disability and for normal people, a demand for intelligent interfaces is ever increasing and therefore related studies are actively being conducted. Recently a study is being conduct...For the convenience of people with disability and for normal people, a demand for intelligent interfaces is ever increasing and therefore related studies are actively being conducted. Recently a study is being conducted to develop an interface through face expression, movement of the body and eye movements, and further more active attempts to use electrical signals(brainwave, electrocardiogram, electromyogram) measured from the human body is also actively being progressed. In addition, the development and the usage of mobile devices and smart devices are promoting these research activities even more. The brainwave is measured by electrical activities between nerve cells in the cerebral cortex using scalp electrodes. The brainwave is mainly used for diagnosis and treatment of diseases such as epilepsy, encephalitis, brain tumors and brain damage. As a result, the brainwave measurement methods and analytical methods were developed. Interface using the brainwave will not go through language or body behavior which is the result of the information processed by the brain but will pass directly to the system providing a brain-computer interface (BCI). This is possible because a variety of the brainwave appears depending on the human’s physical and mental state. Using the brainwave with the intelligent brain-computer interface or combining it with mobile devices and smart devices, regardless of space constraints, the brainwave measurement should be possible.[4,7] In this study, in order to measure the brainwave without spatial constraint, 16 channel compact brainwave measurements system using a high-speed wireless communications were designed. It was designed with a 16 channel to classify the various brainwave patterns that appear and for estimating the location of the nerve cells that triggered the brainwave. And in order to transmit the brainwave data within the channel without loss, a high-speed wireless communication must be possible that can enable a high-speed wireless transmission more sufficient than the Bluetooth, therefore, 802.11 compliant Wi-Fi communication methods were used to transfer the data to the PC. In addition, by using an analog front-end IC having a single-chip configuration with real-time digital filters, the miniaturization of the system was implemented and in order to verify the system Eye-blocking was used to observe the changes in the EEG signal.展开更多
We previously showed that Wnt3a could stimulate human embryonic stem （hES） cell proliferation and affect cell fate determination. In the absence of feeder cell--derived factors, hES cells cultured under a feeder-fre...We previously showed that Wnt3a could stimulate human embryonic stem （hES） cell proliferation and affect cell fate determination. In the absence of feeder cell--derived factors, hES cells cultured under a feeder-free condition survived and proliferated poorly. Adding recombinant Wnt3a in the absence of feeder cell derived-factors stimulated hES cell proliferation but also differentiation. In the present study, we further extended our analysis to other Wnt ligands such as Wntl and Wnt5a. While Wntl displayed a similar effect on hES cells as Wnt3a, Wnt5a had little effect in this system. Wnt3a and Wntl enhanced proliferation of undifferentiated hES cells when feeder-derived self-renewal factors and bFGF are also present. To explore the possibility to promote the proliferation of undifferentiated hES cells by activating the Wnt signaling, we overexpressed Wnt3a or Wntl gene in immortalized human adult fibroblast （HAFi） cells that are superior in supporting long-term growth of undifferentiated hES cells than primary mouse embryonic fibroblasts. HAFi cells with or without a Wnt tmnsgene can be propagated indefinitely. Over-expression of the Wnt3a gene significantly enhanced the ability of HAFi feeder cells to support the undifferentiated growth of 3 different hES cell lines we tested. Co-expression of three commonly-used drug selection genes in Wnt3a-overpressing HAFi cells further enabled us to select rare hES clones after stable transfection or transduction. These immortalized engineered feeder cells （W3R） that co-express growth-promoting genes such as Wnt3a and three drug selection genes should empower us to efficiently make genetic modified hES cell lines for basic and translational research.展开更多
基金This work was supported by the National Research Foundation of Korea(NRF)Grant Funded by the Korea Government(MSIT)(NRF-2020R1C1C1013535).
文摘The complex mechanism of degenerative diseases and the non-specific modulation of regenerative targets aretopics that need to be elucidated in order to advance the use of stem cells in improvement of neurodegenerative diseases.From pre-transplantation through post-transplantation, there are many changes in the conditions, both inside andoutside of the stem cells that have not been carefully considered. This has hindered development in the field of celltherapy and regeneration. This viewpoint highlights the potential implications of intracellular and extracellularalterations of stem cells in transplanted areas at risk of neurodegenerative disease.
文摘For the convenience of people with disability and for normal people, a demand for intelligent interfaces is ever increasing and therefore related studies are actively being conducted. Recently a study is being conducted to develop an interface through face expression, movement of the body and eye movements, and further more active attempts to use electrical signals(brainwave, electrocardiogram, electromyogram) measured from the human body is also actively being progressed. In addition, the development and the usage of mobile devices and smart devices are promoting these research activities even more. The brainwave is measured by electrical activities between nerve cells in the cerebral cortex using scalp electrodes. The brainwave is mainly used for diagnosis and treatment of diseases such as epilepsy, encephalitis, brain tumors and brain damage. As a result, the brainwave measurement methods and analytical methods were developed. Interface using the brainwave will not go through language or body behavior which is the result of the information processed by the brain but will pass directly to the system providing a brain-computer interface (BCI). This is possible because a variety of the brainwave appears depending on the human’s physical and mental state. Using the brainwave with the intelligent brain-computer interface or combining it with mobile devices and smart devices, regardless of space constraints, the brainwave measurement should be possible.[4,7] In this study, in order to measure the brainwave without spatial constraint, 16 channel compact brainwave measurements system using a high-speed wireless communications were designed. It was designed with a 16 channel to classify the various brainwave patterns that appear and for estimating the location of the nerve cells that triggered the brainwave. And in order to transmit the brainwave data within the channel without loss, a high-speed wireless communication must be possible that can enable a high-speed wireless transmission more sufficient than the Bluetooth, therefore, 802.11 compliant Wi-Fi communication methods were used to transfer the data to the PC. In addition, by using an analog front-end IC having a single-chip configuration with real-time digital filters, the miniaturization of the system was implemented and in order to verify the system Eye-blocking was used to observe the changes in the EEG signal.
文摘We previously showed that Wnt3a could stimulate human embryonic stem （hES） cell proliferation and affect cell fate determination. In the absence of feeder cell--derived factors, hES cells cultured under a feeder-free condition survived and proliferated poorly. Adding recombinant Wnt3a in the absence of feeder cell derived-factors stimulated hES cell proliferation but also differentiation. In the present study, we further extended our analysis to other Wnt ligands such as Wntl and Wnt5a. While Wntl displayed a similar effect on hES cells as Wnt3a, Wnt5a had little effect in this system. Wnt3a and Wntl enhanced proliferation of undifferentiated hES cells when feeder-derived self-renewal factors and bFGF are also present. To explore the possibility to promote the proliferation of undifferentiated hES cells by activating the Wnt signaling, we overexpressed Wnt3a or Wntl gene in immortalized human adult fibroblast （HAFi） cells that are superior in supporting long-term growth of undifferentiated hES cells than primary mouse embryonic fibroblasts. HAFi cells with or without a Wnt tmnsgene can be propagated indefinitely. Over-expression of the Wnt3a gene significantly enhanced the ability of HAFi feeder cells to support the undifferentiated growth of 3 different hES cell lines we tested. Co-expression of three commonly-used drug selection genes in Wnt3a-overpressing HAFi cells further enabled us to select rare hES clones after stable transfection or transduction. These immortalized engineered feeder cells （W3R） that co-express growth-promoting genes such as Wnt3a and three drug selection genes should empower us to efficiently make genetic modified hES cell lines for basic and translational research.
