Objective:To evaluate the personality and psychosocial status of adult malocclusion patients through the Eysenck Personality Questionnaire(EPQ) and the Symptom Checklist 90(SCL-90). Methods:The EPQ and the SCL-90 were...Objective:To evaluate the personality and psychosocial status of adult malocclusion patients through the Eysenck Personality Questionnaire(EPQ) and the Symptom Checklist 90(SCL-90). Methods:The EPQ and the SCL-90 were administered to 348 adult respondents with Angle’s ClassⅠ,Ⅱ,Ⅲmalocclusion and normal occlusion.Personality and psychosocial traits were analyzed and compared among the groups.Results:Patients in Angle’s ClassⅠ,D andⅢgroups scored lower on the EPQ-E and higher on the EPQ-N than those in the normal occlusion group, whereas patients in the Class II group scored higher on the EPQ-P.The mean scores of ClassⅠ,ⅡandⅢgroups were significantly higher than those of the normal group on somatization, obsessive-compulsiveness,interpersonal sensitivity,depression,anxiety,and paranoid ideation. All SCL-90 scores were significantly positively correlated with EPQ-N.Psychoticiam and neuroticism scores of female respondents were higher than those of male respondents.The impact of education was greatest on the EPQ-P and the EPQ-E of adult personality,whereas the impact of deformity was greatest on the EPQ-N of adult personality.Conclusions:Personality and psychosocial status show differences in adult patients with ClassⅠ,Ⅱ,Ⅲmalocclusion and normal occlusion and can be influenced by gender,deformity,age and education.展开更多
Objective:To prepare a novel biodegradable poly(2-hydroxyethylmethacrilate)(pHEMAt hydrogel as tissue engineering scaffold.Methods:The pHEMA hydrogel was synthesized by microwaveassisted polymerization using 2-hydroxy...Objective:To prepare a novel biodegradable poly(2-hydroxyethylmethacrilate)(pHEMAt hydrogel as tissue engineering scaffold.Methods:The pHEMA hydrogel was synthesized by microwaveassisted polymerization using 2-hydroxyethyl methacrylale(IIEMA)as the raw material,potassium persulfate as the initiator,and PCI.X as the cross-linking additive.The hvdrogels was characterized with FTIR and NMR spectroscopy.The physical and chemical properties of the prepared hydrogel were evaluated,and its degradation performance was tested.The cytotoxicity of the optimum composite hydrogel was measured by an MTT assay to confirm the feasibility of its use in tissue engineering.Results:The optimum conditions under which the hydrogel was prepared by microwave-assisted polymerization are as follows:1.5 g cross-linking additive,0.3 g initiator,reaction temperature of 80°C,and microwave power of 800 W.Degradation studies showed good degradation profiles with 75%in 17 days.Additionally,the hydrogels did not elicit any cytotoxic response in in vitro cytotoxic assays.Conclusion:A biodegradable pIIEMA hydrogel was successfully prepared by microwave-assisted polymerization,as confirmed from FTIR and NMR results.The hydrogel shows promising applications in tissue engineering,and its healing ability and biocompatibilily will be evaluated in detail in the future.展开更多
There are few studies on the membrane protein Ankfyl. We have found Ankfyl is specifically expressed in neural stem/precursor cells during early development in mice （murine）. To further explore Ankfyl function in ne...There are few studies on the membrane protein Ankfyl. We have found Ankfyl is specifically expressed in neural stem/precursor cells during early development in mice （murine）. To further explore Ankfyl function in neural development, we developed a gene knockout mouse with a mixed Balb/C and C57/BL6 genetic background. Using immunofluorescence and in situ hybridization, neural defects were absent in mixed genetic Ankfyl null mice during development and in adults up to 2 months old. However, Ankfyl gene knockout mice with a pure genetic background were found to be lethal in the C57/BL6 inbred mice embryos, even after seven generations of backcrossing. Polymerase chain reaction confirmed homozygotes were unattainable as early as embryonic day 11.5. We conclude that Ankfyl protein is dispensable in neural stem/precursor ceils, but could be critical for early embryonic murine development, depending on the genetic background.展开更多
The apatite-coated chitosan microspheres were fabricated by incubating chitosan in five times simulated body fluid. The apatite deposited on the microspheres was similar to natural bone mineral, as demonstrated by sca...The apatite-coated chitosan microspheres were fabricated by incubating chitosan in five times simulated body fluid. The apatite deposited on the microspheres was similar to natural bone mineral, as demonstrated by scanning electron microscopy, X-ray diffraction spectra and Fourier transformed-infrared spectroscopy analyses. Rat bone marrow-derived mesenchymal stem cells （BMSCs） were seeded on apatite- coated microspheres to investigate the effect of this scaffold on cell proliferation and differentiation. BMSCs seeded on uncoated microspheres were served as a control. In vivo experiment was evaluated by transplanting the microspheres loaded with or without BMSCs in 5-mm cranial defects of Wistar rats. Bone regeneration was investigated via micro-CT and histological analysis. It was found that apatite-coated chitosan microspheres could significantly promote the proliferation and alkaline phosphatase activity of BMSCs compared with uncoated microspheres. Histological slices and Micro-CT images at 8 weeks revealed much better regeneration of bone in the apatite-coated microspheres loaded with BMSCs than the control. In addition, the defect filled with pure microspheres induced little new bone formation. Our findings suggest that the apatite-coated chitosan microspheres scaffold is a promising carrier of stem cells for cranial bone tissue engineering.展开更多
基金funded in part by a research grant from the Public Health Bureau of Hainan Province(No QW 2007-48)
文摘Objective:To evaluate the personality and psychosocial status of adult malocclusion patients through the Eysenck Personality Questionnaire(EPQ) and the Symptom Checklist 90(SCL-90). Methods:The EPQ and the SCL-90 were administered to 348 adult respondents with Angle’s ClassⅠ,Ⅱ,Ⅲmalocclusion and normal occlusion.Personality and psychosocial traits were analyzed and compared among the groups.Results:Patients in Angle’s ClassⅠ,D andⅢgroups scored lower on the EPQ-E and higher on the EPQ-N than those in the normal occlusion group, whereas patients in the Class II group scored higher on the EPQ-P.The mean scores of ClassⅠ,ⅡandⅢgroups were significantly higher than those of the normal group on somatization, obsessive-compulsiveness,interpersonal sensitivity,depression,anxiety,and paranoid ideation. All SCL-90 scores were significantly positively correlated with EPQ-N.Psychoticiam and neuroticism scores of female respondents were higher than those of male respondents.The impact of education was greatest on the EPQ-P and the EPQ-E of adult personality,whereas the impact of deformity was greatest on the EPQ-N of adult personality.Conclusions:Personality and psychosocial status show differences in adult patients with ClassⅠ,Ⅱ,Ⅲmalocclusion and normal occlusion and can be influenced by gender,deformity,age and education.
基金supported by the National Natural Science Foundation of Hainan Province(Grant No.812200)
文摘Objective:To prepare a novel biodegradable poly(2-hydroxyethylmethacrilate)(pHEMAt hydrogel as tissue engineering scaffold.Methods:The pHEMA hydrogel was synthesized by microwaveassisted polymerization using 2-hydroxyethyl methacrylale(IIEMA)as the raw material,potassium persulfate as the initiator,and PCI.X as the cross-linking additive.The hvdrogels was characterized with FTIR and NMR spectroscopy.The physical and chemical properties of the prepared hydrogel were evaluated,and its degradation performance was tested.The cytotoxicity of the optimum composite hydrogel was measured by an MTT assay to confirm the feasibility of its use in tissue engineering.Results:The optimum conditions under which the hydrogel was prepared by microwave-assisted polymerization are as follows:1.5 g cross-linking additive,0.3 g initiator,reaction temperature of 80°C,and microwave power of 800 W.Degradation studies showed good degradation profiles with 75%in 17 days.Additionally,the hydrogels did not elicit any cytotoxic response in in vitro cytotoxic assays.Conclusion:A biodegradable pIIEMA hydrogel was successfully prepared by microwave-assisted polymerization,as confirmed from FTIR and NMR results.The hydrogel shows promising applications in tissue engineering,and its healing ability and biocompatibilily will be evaluated in detail in the future.
基金Dr.Hui Fu was supported by the National Natural Science Foundation of China,No.81371338by Open Research Fund Program of Hubei-MOST KLOS & KLOBMEDr.Zu-neng Lu was supported by grants from Health and Family Planning Commission of Hubei Province scientific research project,No.WJ2015MA007
文摘There are few studies on the membrane protein Ankfyl. We have found Ankfyl is specifically expressed in neural stem/precursor cells during early development in mice （murine）. To further explore Ankfyl function in neural development, we developed a gene knockout mouse with a mixed Balb/C and C57/BL6 genetic background. Using immunofluorescence and in situ hybridization, neural defects were absent in mixed genetic Ankfyl null mice during development and in adults up to 2 months old. However, Ankfyl gene knockout mice with a pure genetic background were found to be lethal in the C57/BL6 inbred mice embryos, even after seven generations of backcrossing. Polymerase chain reaction confirmed homozygotes were unattainable as early as embryonic day 11.5. We conclude that Ankfyl protein is dispensable in neural stem/precursor ceils, but could be critical for early embryonic murine development, depending on the genetic background.
基金Funded by the National Natural Science Foundation of China （30700176） and the Provincial Natural Science Foundation of Hubei Province （2011CDB470）
文摘The apatite-coated chitosan microspheres were fabricated by incubating chitosan in five times simulated body fluid. The apatite deposited on the microspheres was similar to natural bone mineral, as demonstrated by scanning electron microscopy, X-ray diffraction spectra and Fourier transformed-infrared spectroscopy analyses. Rat bone marrow-derived mesenchymal stem cells （BMSCs） were seeded on apatite- coated microspheres to investigate the effect of this scaffold on cell proliferation and differentiation. BMSCs seeded on uncoated microspheres were served as a control. In vivo experiment was evaluated by transplanting the microspheres loaded with or without BMSCs in 5-mm cranial defects of Wistar rats. Bone regeneration was investigated via micro-CT and histological analysis. It was found that apatite-coated chitosan microspheres could significantly promote the proliferation and alkaline phosphatase activity of BMSCs compared with uncoated microspheres. Histological slices and Micro-CT images at 8 weeks revealed much better regeneration of bone in the apatite-coated microspheres loaded with BMSCs than the control. In addition, the defect filled with pure microspheres induced little new bone formation. Our findings suggest that the apatite-coated chitosan microspheres scaffold is a promising carrier of stem cells for cranial bone tissue engineering.
